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1.
Changes in teat skin surface pH were studied over 12 wk in 99 lactating Holstein cows. Half the udder of each cow routinely received postmilking disinfection, and the other half served as control. Measures of pH were made on all teats at weekly intervals. Teat skin pH was affected by treatment but not week. Mean teat skin pH measures were: 7.18 (+/- 0.64) and 7.53 (+/- 0.46) for treatment and control teats. In study II, pH teat skin measures were made hourly on 16 cows, starting 2 h before milking, immediately before a milking, immediately after a milking, and for 2 h postmilking. Teat skin pH was significantly lower for treatment teats and was lower for all teats postmilking. 相似文献
2.
Galton DM 《Journal of dairy science》2004,87(1):225-231
A technology of automatically applying a postmilking teat dip via the milking machine prior to machine detachment was compared to manual postmilking teat dipping with a teat dip cup for effects on new IMI and iodine content in milk. One hundred twenty Holstein cows were experimentally challenged in a 22-wk trial with Streptococcus agalactiae and Staphylococcus aureus and 148 Holstein cows were experimentally challenged with Streptococcus uberis in another 22-wk trial. The bacterial suspensions were applied to teats of all of the cows after premilking udder preparation and immediately prior to milking machine attachment. In both trials, cows were divided among four treatments: no postmilking teat dipping; manual postmilking teat dipping with a proven efficacious iodophor teat dip; manual postmilking teat dipping with an iodophor teat dip formulated for an automatic postmilking teat dipping system; and automatically postmilking teat dipping via milking machines with an iodophor teat dip formulated for the automatic postmilking teat dipping system. The postmilking teat dipping treatments reduced new Staph. aureus IMI by 64.5, 76.5, and 88.2%; new Strep. agalactiae IMI by 61.5, 77.8, and 94.4%; and new Strep. uberis IMI by 63.5, 82.5, and 93.8%, respectively, against the treatment of no postmilking teat dipping. The treatment applying the postmilking teat dip automatically via milking machines had the lowest number of new IMI caused by the three pathogens. Teat end and teat skin condition were characterized as normal at the end of the study with no differences between treatments. There were no differences with regard to iodine content in milk between treatments. 相似文献
3.
A 1.0% iodophore teat disinfectant (Full-Bac) was evaluated in comparison with a positive control (Bovadine), a commercially available 1.0% iodophore teat disinfectant. The study was conducted under conditions of experimental challenge, following the guidelines recommended by the National Mastitis Council. The test product and a positive control were compared in 41 cows, with 82 teats receiving each product at milking, during a 10-wk study period. There were no differences between the test product and the positive control in new intramammary infections due to Staphylococcus aureus, which averaged 13.4% in each of the 2 treatment groups. Additionally, no statistical difference was seen between the test product and positive control in new intramammary infections by Streptococcus agalactiae, which averaged 8.5 and 6.1% for the Full-Bac and Bovadine groups, respectively. Teat skin and teat end condition scores were statistically evaluated at wk 1, 5, and 9 of the study, and no significant differences were observed between the treatment groups. The test teat disinfectant provided similar germicidal activity to that of the positive control teat disinfectant with no adverse effects on teat skin or teat end condition during the warm-season study period. 相似文献
4.
J.M. Melvin W. Heuwieser P.D. Virkler D.V. Nydam M. Wieland 《Journal of dairy science》2019,102(3):2657-2669
Changes in teat canal diameter as induced by machine milking are thought to be related to teat canal penetrability and susceptibility to new intramammary infections. The objective of this study was to describe the effects of machine milking on teat canal dimensions as assessed by ultrasonography and to evaluate postmilking changes of teat canal dimensions throughout an 8-h milking interval. Ultrasonographic images were taken of the left front and right hind teats of 80 Holstein cows milked 3 times per day. Imaging occurred before attachment of the milking unit after completion of premilking udder preparation (t?1), immediately after removal of the milking unit (t0), and then every hour after milking until 8 h had elapsed (t1 to t8). Teat canal length and teat canal diameter (at the proximal, middle, and distal regions) were measured. General linear mixed model analysis indicated differences in the relative change of teat canal length (compared with t?1) with least squares means (LSM) and 95% confidence interval (95% CI) of 11.1% (8.7–13.4%) after machine milking (t0). Teat canal length decreased throughout the 8-h milking interval but remained elevated relative to t?1. Teat canal diameter at the proximal and middle regions increased near the end of the milking interval, whereas that at the distal region did not change meaningfully during the 8 h of observation. Our findings suggest that the teat canal and its surrounding tissue is in a state of near-constant remodeling in cows milked 3 times daily. Future work is warranted to study the association between ultrasonographically assessed changes of teat canal dimensions after machine milking and teat canal penetrability to mastitis-causing pathogens. 相似文献
5.
Preventing bovine mastitis by a postmilking teat disinfectant containing acidified sodium chlorite 总被引:1,自引:0,他引:1
A split-herd study was performed to determine if an acidified, sodium chlorite teat disinfectant, UDDERgold Platinum Germicidal Barrier Teat Dip (UG Pt, Ecolab Inc., Redmond, WA), was effective in preventing new intramammary infections (IMI) in lactating dairy cows compared with a licensed, iodophor teat disinfectant (Iosan, Novartis Animal Health, Ltd., Whittlesford, UK), and to show that the test product was tolerated equally well by teat skin. The study lasted 114 d and covered all weather conditions. The teats of 176 cows were dipped after each milking in UG Pt and the teats of 172 cows were dipped in Iosan, the positive-control product. Routine milk samples were taken from each quarter of every cow every 4 wk. Additional samples were taken from newly calved cows joining the trial and from cows with clinical signs of mastitis. Milk samples were cultured for the presence of bacteria and the cause of clinical mastitis. Each quarter was eligible for only 1 infection during the trial. The number of clinical cases was identical in each group (n = 13) and the number of subclinical infections was slightly lower in the UG Pt group than in the Iosan group (n = 27 and 31, respectively). These rates of infection suggest that the products did not differ in their ability to prevent a new IMI. At least 203 cows were assessed for skin integrity before the start of the trial and every 28 d throughout. The UG Pt teat dip had no adverse effects on teat condition. The prevalence of hyperkeratosis did not change with time for both groups (0.90 ± 1.08 and 0.95 ± 1.06 at wk 0 vs. 0.65 ± 0.87 and 0.49 ± 0.74 at wk 16 for fore and hind teats, respectively, for UG Pt and 1.02 ± 1.25 and 1.16 ± 1.11 at wk 0 vs. 0.51 ± 0.71 and 0.45 ± 0.65 at wk 16, respectively, for Iosan); no redness of the skin was observed in either group. Application of recommended statistical methods to demonstrate noninferiority was problematic. 相似文献
6.
Bacterial populations of teat skin are associated with risk of intramammary infection and may be influenced by anatomical characteristics of teats. The objective of this study was to evaluate associations of selected anatomical characteristics of teats with bacterial counts of teat skin of cows exposed to different types of bedding. Primarily primiparous Holstein cows (n = 128) were randomly allocated to 4 pens within a single barn. Each pen contained 1 type of bedding [new sand (NES), recycled sand (RS), deep-bedded manure solids (DBMS), and shallow-bedded manure solids over foam core mattresses (SBMS)]. During a single farm visit udders (n = 112) were scored for hygiene and 1 front (n = 112) and 1 rear teat (n = 111) of each enrolled cow were scored for hyperkeratosis (HK). Teat length, teat barrel diameter, and teat apex diameter were measured and teat skin swabs were systematically collected for microbiological analysis. Linear type evaluation data for udders of each cow were retrieved for each cow. Teat position (front or rear) was associated with occurrence of clinical mastitis during the 12 mo before the farm visit and more cases occurred in front quarters. The proportion of udders that were classified as clean (score 1 or 2) was 68, 82, 54, and 95% for cows housed in pens containing NES, RS, SBMS, and DBMS, respectively. No association was found between HK score and teat position and no association was found between HK score and teat skin bacterial count. Bacterial counts of teat skin swabs from front teats of cows in pens containing RS and SBMS were significantly less than those of rear teats of cows in pens containing DBMS or NES. Teat skin bacterial counts were significantly greater for swabs obtained from teats of cows with udder hygiene scores of 3 and 4 as compared with swabs obtained from cows with cleaner udders. Of all udder conformation traits evaluated, only narrower rear teat placement was positively associated with bacterial counts on teat skin. 相似文献
7.
A teat-dip formulation containing sodium dichloro isocyanuric acid, bronopol, and quaternary ammonium was tested for efficacy against Staphylococcus aureus and Streptococcus agalactiae intramammary infections (IMI) using an experimental challenge model. Sixty-two Jersey cows from the Hill Farm Research Station (Homer, LA) were used in an 8-wk controlled infection trial to evaluate the teat dip. During the afternoon milking, Monday through Friday for 8 wk, all teats of each cow were immersed to a depth of approximately 25 mm in a challenge suspension containing approximately 5 x 10(7) cfu of Staphylococcus aureus and approximately 5 x 10(7) cfu of Streptococcus agalactiae immediately after milking machines were removed. Immediately after challenge, the distal 25 mm of two contralateral teats were dipped with the experimental teat dip; the remaining two teats served as undipped controls. The experimental teat dip reduced the number of new Staph. aureus IMI by 70.9% and reduced the number of new Strep. agalactiae IMI by 60.0%. Teat end and teat skin condition were characterized as normal and without irritation at the completion of the study. The combination of the three germicides in this experimental teat dip is unique and an effective formulation without adverse effects on condition of teat ends or teat skin. 相似文献
8.
To examine the development of teat end callosity thickness and roughness in early lactation and to quantify cow factors of interest, a system to classify teat end condition was developed. A distinction was made between rough and smooth rings around the teat orifice. In addition, a classification of the degree of callosity was developed. Kappa coefficients for the repeatability of scoring by this classification system by different workers were 0.71 for teat end callosity thickness and 0.86 for teat end callosity roughness. The teat end callosity classification system was used for a longitudinal study with 40 cows during the first 14 wk of lactation. Models were built to predict teat end callosity thickness and roughness, machine-on time, and milk yield. For the response variables, teat end callosity thickness, machine-on time, and milk yield, the consecutive measurements appeared to follow a lactation curve model with a subject-specific general slope and intercept. Teat end callosity increased rapidly the first 8 wk. Cow factors such as days in milk, parity, machine-on time, and teat end shape were associated with the degree of teat end callosity, and the probability of the callosity ring to become rough. Teat end callosity thickness did not decrease within the 14-wk trial period for most teats. Pointed or round teat ends showed more callus than inverted teat ends. Longer machine-on time resulted in a higher probability of the callosity ring to become rough. Rear teats showed less callosity than front teats in this study. 相似文献
9.
Technical success and effectiveness of teat cleaning and the management factors associated with them were evaluated in 9 automatic milking herds. In total, 616 teats cleaned with a cleaning cup and 716 teats cleaned with rotating brushes were included. Technical success and the effectiveness of teat cleaning, including the location and nature of the dirt, were evaluated visually. On average, 79.9% of teat cleanings with a cleaning cup, and 85.0% of those cleaned with brushes succeeded technically; that is, the teat was correctly positioned in the cleaning device throughout the whole cleaning process. The difference between use of teat cups and brushes was significant. However, because technical success of teat cleaning is strongly dependent on herd characteristics, these results should be interpreted with caution. Factors associated with the technical success of teat cleaning with a cleaning cup were herd, days in milk, behavior of the cow, teat color, and teat location. For rotating brushes, behavior of the cow, teat location, udder and teat structure, and days in milk were associated with technical success. Excessive udder hair and technical failure of the automatic milking machine also caused a few technically unsuccessful teat cleanings with a cleaning cup. Teats with technically successful teat cleanings were evaluated for the effectiveness of teat cleaning. From originally dirty teats, the cleaning cup had a significant advantage over the brushes in the percentage of teats that became clean or almost clean during the cleaning process (79.8 vs. 72.9%). Teat orifices were least effectively cleaned compared with the teat barrel and apex. Bedding material (peat, sawdust, or straw) on the teat was cleaned almost completely. Factors associated with the effectiveness of teat cleaning were teat cleanliness before cleaning, herd, teat cleaning method, and teat condition. The variation among herds indicates the likelihood that herd management factors can be adjusted to improve milking hygiene. There is also a need to improve the precision and effectiveness of the teat cleaning mechanisms of automatic milking systems. 相似文献
10.
Teat cup liner slips, manual milking machine adjustments, milk yields, and milking times were recorded during both morning and evening milkings for 8 d on 97 Holstein cows in The Pennsylvania State University dairy herd. Fore and rear udder heights (distance from floor to udder), udder levelness, distances between teats (before and after milking), teat lengths, teat diameters, and teat end shapes were measured on the same cows. Product-moment correlations among the morphological characteristics, linear slips, manual adjustments, milk yields, and milking times were determined. Residual correlations from a model including lactation number and DIM (linear and quadratic) were also calculated. The variation among cows in machine liner slips and manual adjustments within and across lactation number and DIM can be partially explained by udder and teat morphology. Wider teats were associated with increased linear slips and increased manual adjustments. More tilted udders (rear quarters lower than front quarters) were associated with increased liner slips and tended to be associated with increased manual adjustments. In addition, larger teat diameters and longer teats tended to be associated with increased liner slips. 相似文献
11.
12.
An experimental teat dip containing 0.5% hydrogen peroxide as the active ingredient was compared with a teat disinfectant also containing 0.5% hydrogen peroxide that is commercially available throughout North America. The study was conducted under conditions of experimental challenge with a positive control following the guidelines recommended by the National Mastitis Council. The efficacy of the test product and the positive control product were compared in 45 cows, with 89 total teats receiving each product after milking during a 10-wk study period. There was no significant difference between the experimental hydrogen peroxide product and the positive control in new intramammary infections caused by Staphylococcus aureus (27.0 and 18.0% in the treatment groups, respectively). Additionally, there was no significant difference between the experimental product (6.7%) and the positive control groups (4.5%) in new intramammary infections caused by Streptococcus agalactiae. Traditional analysis of teat skin condition changes supported improved teat skin condition with the test disinfectant. After accounting for the correlation of teats within cow, significant differences were also observed between the treatment groups for teat skin condition. The experimental hydrogen peroxide-based teat disinfectant provided efficacy similar to that of the positive control teat disinfectant, with significant improvement in teat skin condition and no adverse effects on teat end condition. 相似文献
13.
Claudia Stauffer Elke Van der Vekens Michael H. Stoffel Daniela Schweizer Rupert M. Bruckmaier 《Journal of dairy science》2021,104(8):9082-9092
Transiently increased teat wall thickness in response to machine milking has been documented by various methods, including ultrasound. However, correlative ultrasonography and histology to detect the origin of this phenomenon is lacking. The first goal of the present study was to evaluate and compare milking-related changes of the teat tissue in 2 breeds of dairy cows (11 Simmental and 3 Holstein) using B-mode ultrasonography. Additionally, the observed changes were compared with ultrasonographic findings in a Holstein cow with periparturient udder edema. Finally, corresponding histological sections of the Simmental teats were analyzed and compared with those from a lactating nonmilked Angus cow. We hypothesized that the mechanical load of both stretching by the vacuum during phases of open teat cup liner and compression by the closed liner during machine milking results in a transient congestion of blood vessels in the teat wall. The barrel of 1 front teat of each cow was scanned immediately before and after machine milking (system vacuum: 42 kPa; pulsation rate: 60 cycles/min; pulsation ratio: 65:35). Shortly after milking (33 ± 6 min), the Simmentals were slaughtered, and their scanned teat was immediately removed and processed for investigation by light microscopy. Ultrasonography after milking revealed anechoic tubular structures mainly in the inner half of the teat wall. Histological examination revealed these structures to be thick-walled veins. The left front and hind teats of the nonmilked lactating cow, collected and prepared identically to those from the Simmental cows, showed the same histological features. Ultrasonographic measurements showed that the diameter of these veins significantly increased after milking compared with matching images before milking. This effect was most pronounced in the Holstein cows. Similarly, these veins were very prominent in the periparturient cow. However, neither the milked cows, including the periparturient cow, nor the lactating nonmilked cow provided any evidence of edematous extravasation on ultrasonography or histology. These findings corroborated our hypothesis that the increase in size of thick-walled veins in the teat tissue is the main reason for the thickening of the teat walls in response to machine milking. 相似文献
14.
D.J. Reinemann B.H.P. van den Borne H. Hogeveen M. Wiedemann C.O. Paulrud 《Journal of dairy science》2021,104(6):6820-6831
The objective of this study was to compare a vacuum control system that increases milking system vacuum during the peak flow period of milking to conventional constant vacuum control technology regarding its effect on milk flowrate and milking duration. Further objectives were to study the effects of flow-controlled vacuum on milking parlor performance. An observational study was conducted on a commercial dairy farm milking from 848 to 896 cows per day over the study period using a 60-stall rotary milking parlor. The flow-controlled vacuum control system was applied for 3 wk. Milking performance and teat condition were compared with 3-wk periods prior and subsequent to the test period using conventional vacuum control. Statistical analysis was performed assuming a cross-sectional study design during each period. Flow-controlled vacuum increased peak milk flowrate by 12% and increased average milk flowrate by 4%. The decrease in individual cow milking duration was proportional to milk yield per milking. Postmilking teat condition was good during the entire study period. The occurrence of rough teat ends was slightly reduced during the flow-controlled vacuum period with no meaningful difference in the occurrence of teats with blue color, palpable rings, or petechia. The combination of reduced vacuum during the low flow period of milking and the decrease in milking duration are likely factors that are protective of teat tissues. Bioeconomic modeling of the use of flow-controlled vacuum on the performance of rotary milking parlors, using the data that were collected during the study, showed that the reduction in milking duration of individual cows allows a higher rotary parlor speed. Modeled parlor throughput increased by 5.0% to 419 cows/h, 6.8% to 407 cows/h, and 4.2% to 326 cows/h when 80%, 95%, and 99% of the cows were finished milking at the end of the rotation for a 60-stall parlor. Model results showed that increased parlor throughput resulted in increased labor efficiency, reduced labor costs for milking, and a positive benefit-cost ratio on the investment for all but the smallest herd and parlor sizes considered. 相似文献
15.
K.M. Morrill J.C. Scillieri Smith H.M. Dann H.M. Gauthier C.S. Ballard 《Journal of dairy science》2019,102(3):2507-2514
The objective of this trial was to compare a powdered 0.5% chlorhexidine acetate-based postmilking teat dip with a foamed 1% iodine-based postmilk teat dip during winter on clinical mastitis, subclinical mastitis (somatic cell count ≥200,000 cells/mL), linear score, teat skin condition, teat end score for hyperkeratosis, and risk of developing a new intramammary infection (IMI). Holstein cows (n = 331) housed in freestall and tiestall barns on one farm were blocked by pen, parity, lactation stage, and lactation performance. They were assigned randomly to a powdered chlorhexidine postmilking teat dip (PD; Derma Soft n' Dry, IBA Inc., Millbury, MA) or a foamed iodine-based postmilking teat dip (ID; FS-103, IBA Inc.). Treatments were applied for 6 wk starting January 4, 2016, for 3 milkings per day. Milk samples were collected from each quarter at the beginning and end of the trial and analyzed for aerobic culture and somatic cell count. Cows that had a clinical mastitis event during the trial were quarter sampled for aerobic culture at the time of clinical event. Teat skin condition and teat end score for hyperkeratosis were evaluated at the beginning, middle, and end of the trial based on a 3- and 5-point scale, respectively. No treatment difference was observed for linear score or teat skin condition. Teat end score was greater for ID cows compared with PD cows (2.72 vs. 2.77) at the conclusion of the trial. At the beginning of trial 102 PD quarters and 129 ID quarters had an IMI identified on aerobic culture, 402 PD and 457 ID quarters cultured negative, and 109 PD and 125 ID samples were classified as “no significant growth.” At the conclusion of the trial, 129 PD and 101 ID quarters had an IMI. Use of PD resulted in a greater risk for developing a new IMI, based on bacteriological culture, at the conclusion of the trial as compared with ID (relative risk = 1.51; confidence interval: 1.10–2.07). Additionally, use of PD resulted in a greater risk as compared with ID of coagulase-negative staphylococci (relative risk = 1.5; confidence interval: 1.10–2.25) and Staphylococccus aureus (relative risk = 2.30; confidence interval: 1.04–5.07) to be present at the conclusion of the trial. In conclusion, use of PD led to a lower teat end score, an increase in new IMI, and an increased risk of coagulase-negative staphylococci and Staph. aureus compared with ID after 6 wk of product use. 相似文献
16.
《Journal of dairy science》2023,106(9):6342-6352
The aim of this study was to perform a positive-controlled field study under natural exposure conditions to test the efficacy of a newly developed chlorine dioxide-based postmilking teat disinfectant (experimental product, EX) for noninferiority compared with an already established chlorine dioxide-based teat disinfectant (positive control product, PC). After blocking by parity, approximately 200 Holstein cows in early to mid-lactation stages from a dairy farm near Padua, Italy, were randomly assigned to one of 2 groups. Over a 13-wk period between September and December 2021, the teats of cows were dipped with the EX or the PC after each milking. Milk samples were collected from individual quarters of enrolled cows for 13 wk to determine infection status. Teat condition was assessed at wk 1, 5, and 9. Mixed logistic regression was used to analyze the effect of treatment on the incidence of new intramammary infections. For the noninferiority analysis, the upper limit of the 95% confidence interval for the difference in new intramammary infection (NIMI) rate between the 2 treatments (EX − PC) had to be to the left of the critical value d (0.035) to conclude that EX was noninferior to PC in terms of the risk of NIMI. The results showed that the incidence of new infections in the quarters treated with EX (3.1%) was not different from that in the udder quarters treated with PC (2.6%). No overall difference was found between the treatments in terms of teat condition. As the upper limit of the 95% confidence interval of the NIMI rate difference was smaller than the predefined noninferiority limit, we concluded that the EX was noninferior compared with the PC. 相似文献
17.
Teat anatomy and its relationship with quarter and udder milk flow characteristics in dairy cows 总被引:1,自引:0,他引:1
Anatomical and functional characteristics of the teat are supposed to have considerable influence on milk flow performance. In the present study, various teat and milking characteristics in 148 quarters of 38 cows were analyzed via 3 different approaches. Teat canal length, teat wall thickness, and teat diameter were measured by ultrasound. In addition, the vacuum needed to open the teat canal (VO) was determined and milk flow profiles were measured in each quarter separately.Rear teats were shorter and thicker than front teats, whereas teat canal length and teat wall thickness did not differ according to quarter position. Milk yield and peak flow rate (PFR) were higher in rear than in front quarters. Teat canal length and VO were negatively correlated with PFR and average flow rate (AFR) but no correlations were observed between milkability traits and externally measurable teat characteristics like teat length or teat diameter.Individual milkability at an udder level is a complex characteristic that is determined by the milkability at a quarter level and the distribution of quarter milk yields. The anatomical and functional characteristics of single teats can partly explain the milk flow characteristics of individual quarters. 相似文献
18.
Zdanowicz M Shelford JA Tucker CB Weary DM von Keyserlingk MA 《Journal of dairy science》2004,87(6):1694-1701
The main objectives of the experiment were: 1) to compare bacterial populations of mastitis-causing organisms on the teats of lactating dairy cattle housed on sand and sawdust bedding and, 2) to examine the relationship between bacterial counts present in the 2 bedding types with those on teat ends. Sixteen lactating Holstein cows were housed on either sand or sawdust-bedded free stalls using a crossover design with 3 wk per bedding type. Bedding samples were collected on d 0 (prior to animals lying on the bedding), 1, 2, and 6. Teat ends were sampled prior to the morning milking on d 1, 2, and 6. All samples were analyzed to determine coliform, Klebsiella spp., and Streptococcus spp. populations. There were 2 times more coliforms and 6 times more Klebsiella bacteria on teat ends of cows housed on sawdust compared with those housed on sand. In contrast, there were 10 times more Streptococcus spp. bacteria on teat ends of cows when housed on sand compared with sawdust. In both sawdust and sand bedding, coliforms, Klebsiella and Streptococcus counts increased over each experimental week, although patterns varied with bedding and bacteria type. Bacterial counts on teat ends were correlated with bacterial counts in sawdust (r = 0.47, 0.69, and 0.60 for coliforms, Klebsiella spp., and streptococci, respectively) and in sand (r = 0.35 for coliforms and r = 0.40 for Klebsiella spp.). In conclusion, coliforms and Klebsiella spp. on teat ends were more numerous when cows were housed on sawdust bedding, but Streptococcus spp. were more numerous on teat ends of cows housed on sand. 相似文献
19.
M. Wieland J.M. Melvin P.D. Virkler D.V. Nydam W. Heuwieser 《Journal of dairy science》2018,101(2):1518-1523
The objectives were to (1) develop a standard operating procedure (SOP) for ultrasound-based measurements of teat canal dimensions and (2) determine the precision of ultrasound-based measurements of teat canal dimensions in dairy cows. Teat scans (n = 64) of the right hind and left front teats from 16 cows were obtained with a portable ultrasound device before and after machine milking. Teat dimensions measured were teat canal length (TCL), teat canal diameter at the proximal end of the teat canal (TCDPROX), teat canal diameter at the midpoint between the proximal and distal ends of the teat canal (TCDMID), teat canal diameter at the distal end of the teat canal (TCDDIS), teat end diameter at the midpoint between the proximal and distal ends of the teat canal (TMD), and teat canal cross-sectional area (TCLAREA). An SOP was developed, reviewed, and modified by 3 operators. Measurements were performed by the same 3 operators using an open source software program. To assess the effect of implemented modifications and the precision of ultrasound-based measurements, concordance correlation coefficients (CCC) were calculated to determine interoperator reproducibility and intraoperator repeatability. Through modifications in the SOP, interoperator CCC increased from 0.45 to 0.86 for TCL, from 0.14 to 0.66 for TCDPROX, from 0.24 to 0.66 for TCDMID, from 0.06 to 0.56 for TCDDIS, from 0.64 to 0.91 for TMD, and from 0.17 to 0.64 for TCLAREA. Intraoperator CCC over all operators were 0.91 for TCL, 0.73 for TCDPROX, 0.80 for TCDMID, 0.69 for TCDDIS, 0.94 for TMD, and 0.75 for TCLAREA. Our results indicate that measurements of teat canal dimensions can be conducted with satisfactory precision when following an SOP. Development and strict implementation of an SOP has the potential to decrease inadvertent variability of ultrasound-based measurements among operators for ultrasonographic assessment of teat dimensions. 相似文献
20.
M. Wieland D.V. Nydam N. Älveby P. Wood P.D. Virkler 《Journal of dairy science》2018,101(12):11447-11454
Machine milking-induced changes in teat tissue condition, such as congestion and edema, have been associated with teat canal openness, penetrability, and thus susceptibility to new intramammary infections and diminished animal well-being. The primary objective of this study was to investigate the association of teat-end shape and machine milking-induced short-term changes in teat tissue condition. Our secondary objective was to study the association of udder-level milking characteristics and short-term changes. Data from 125 Holstein cows were analyzed in a longitudinal prospective cohort study. Cows were housed in freestall pens with sand bedding, fed a total mixed ration, and milked 3 times per day. Teat-end shape was classified into 3 categories: pointed, flat, and round. Udder-level milking characteristics were obtained from electronic on-farm milk meters. Short-term changes in teat tissue condition after machine milking were assessed visually. Multivariable generalized mixed model analysis showed an association between teat-end shape and short-term changes. Compared with teats with round teat-end shape, the odds ratio (95% confidence interval) for short-term changes was 1.68 (0.53–5.31) and 0.03 (0.004–0.19) in teats with pointed and flat teat-end shape, respectively. There was an association between milking characteristics and short-term changes such that higher milk flow rate during the first 15 s of milking decreased the likelihood of short-term changes. The adjusted probability of short-term changes for a milking observation of a mid-lactation cow and an average first 15-s milk flow rate of 0.5 and 1.5 kg/min was 53.0% (42.8–63.8) and 32.9% (15.2–57.3), respectively. Our results suggest that teat-end shape may be one of the risk factors that contribute to machine milking-induced short-term changes. Milking characteristics (e.g., first 15-s milk flow rate) may have the potential as a measure to indirectly monitor teat tissue changes associated with machine milking on a daily basis, though further research is needed to validate this hypothesis and to establish thresholds that could serve as on-farm guidelines. 相似文献