共查询到17条相似文献,搜索用时 62 毫秒
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通过柱色谱法从马铃薯叶的提取物中分离提纯了茄尼醇,并以RP-HPLC法测定了茄尼醇的质量分数。采用硅胶对茄尼醇进行吸附纯化,以茄尼醇的实测收率、质量分数为考察指标,通过对硅胶颗粒、洗脱剂流速、洗脱剂组分配比进行考察,确定了茄尼醇的柱色谱优化条件。结果表明,以200~300目硅胶为填料,流动相流速为2.0 mL/m in,洗脱剂以石油醚、V(石油醚)∶V(乙酸乙酯)=20∶1、15∶1、9∶1进行梯度洗脱,茄尼醇的质量分数达到85.37%,重结晶后达到97.47%。 相似文献
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硅胶柱层析法提纯大豆磷脂酰胆碱的研究 总被引:2,自引:0,他引:2
探讨了以大豆浓缩磷脂为原料,应用柱层析法提纯大豆磷脂酰胆碱(PC)的工艺过程.用TLC法考察了水、异丙醇与正己烷的体积配比,确定流动相适宜配比为正己烷:异丙醇:水(1:1:0.175,v/v/v).通过对固定相、流动相和上载量的考察,得到了柱层析最佳工艺条件:在空塔流速为0.382 cm·min-1,进样浓度为0.25 g浓缩磷脂·(mL流动相)-1的室温条件下,固定相选用100~200目粗孔2号硅胶,流动相为正己烷:异丙醇:水(1:1:0.16,v/v/v),最适上载量为0.086gPC·(g硅胶)-1.经高效液相色谱法定量分析,产品中磷脂酰胆碱纯度高达85%. 相似文献
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应用柱层析法纯化Vero细胞乙型脑炎疫苗 总被引:1,自引:3,他引:1
浓缩Vero细胞乙脑疫苗经SepharoseCL-6B柱层析可去除大部分杂蛋白,再经SphacrylS-500HR层析可进一步去除比病毒大的杂质及残余小分子量杂蛋白。经两步凝胶过滤层析纯化后,疫苗总蛋白含量减少约99%,抗原比活性提高87~165倍,抗原回收率达30%~50%,残余牛血清和残余细胞DNA均符合WHO有关规程要求,提纯疫苗的效力达到或超过中国地鼠肾灭活乙脑疫苗和日本提纯鼠脑拔苗的参考苗。 相似文献
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柱层析法纯化基因工程HBsAg中试工艺的研究 总被引:5,自引:5,他引:5
由乙肝病毒S基因转化的哺乳动物细胞培养收液,经过Butyl-S-SepharoseFF层析,DEAESepharoseFF层析和Sepharose4FF层析,可获HBsAg纯品。产品检定结果表明,PAGE和SDS-PAGE电泳纯度均合格,小牛血清残余量、细胞DNA残余量和动物免疫效力也均符合规定标准.HBsAg终收率达40%左右.在此工艺中,疏水作用柱层析的纯化效率比较高,可去除绝大部分杂蛋白和细胞DNA。 相似文献
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用球状4A(钠A)型分子筛作固定相,以无水乙醇作流动相,采用迎头法洗脱,对无水乙醇中的甲醇进行柱层析,制备了无甲醇乙醇。 相似文献
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Leslie Kleiner-Shuhler Luis Vázquez Casimir C. Akoh 《Journal of the American Oil Chemists' Society》2011,88(8):1161-1171
The objective of this study was to purify stearidonic acid (SDA, 18:4 ω-3) from modified soybean oil containing a mixture
of over 20 fatty acids (23% SDA). Interest in obtaining purified fractions of SDA arises from reported health benefits associated
with polyunsaturated fatty acids (PUFA), such as cardiovascular disease prevention. In addition, SDA may also provide improved
stability characteristics since its unsaturation index is less than longer PUFA, such as eicosapentaenoic acid (EPA, 20:5
ω-3). First, a chemical ethanolysis of modified soybean oil was performed to transform the triacylglycerols into fatty acid
ethyl esters (FAEE). Then, the FAEE were fractionated and SDA-EE was purified by argentation silica gel (10% AgNO3) open column chromatography, which allows selectivity based on degree of unsaturation. Different FAEE sample loads and mobile
phases were explored until the best purification of SDA-EE was achieved. The solvents used were hexane and hexane:acetone
mixtures (99 and 95%). Under the optimal conditions, a fraction with high SDA-EE purity (96%) was obtained with 77% yield.
Besides, it was possible to obtain another fraction enriched in α-linolenic acid-EE (37% purity and 68% yield) and γ-linolenic
acid-EE (22% purity and 61% yield). A scaled-up process resulted in 840 mg of final product composed of 97% SDA-EE with 71%
yield. 相似文献
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从辣椒素总碱(Capsaicinoids)中提取高纯度的辣椒索及二氢辣椒素单体,建立了分离工艺条件。以TLC实验筛选适当的洗脱流动相,低压硅胶柱层析分离纯化,产物用两相法重结晶,TLC及HPLC监测分离效果及产品纯度。结果表明优选的流动相为混合溶剂石油醚:丙酮=8:2(体积比),纯化分离得到辣椒素和二氢辣椒索,纯度分别从64%和29%提高到97%和96%。 相似文献
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M. A. Rincón-Cervera I. Rodríguez-García J. L. Guil-Guerrero 《Journal of the American Oil Chemists' Society》2009,86(7):605-609
Gravimetric normal-phase silver ion–silica gel column chromatography has been used for the novel application of purification
of GLA-containing triglycerides (GLA-TGs) from evening primrose seed oil (EPO). Gradient elution with increasing polarity
enabled separation of valuable TG species containing γ-linolenic acid (GLA, 18:3n-6). Enzymatic hydrolysis revealed the distribution of fatty acids (FAs) in the isolated TG species,
with GLA in the sn-2 position in different percentages, depending on the degree of unsaturation. A novelty of this work was the successful use
of the procedure to improve the purification of raw GLA species from EPO up to preparative scale, thus enabling use of this
methodology for industrial purposes. 相似文献