共查询到19条相似文献,搜索用时 62 毫秒
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膜生物反应器(MBR)被广泛应用于污水的深度处理和回用。然而,膜表面的生物污染一直是MBR应用中的难题,至今仍未得到有效解决。研究结果表明,生物膜的形成与细胞间的群体感应(QS)有关,因此,通过干扰QS系统而阻止生物膜形成的群体淬灭(QQ)技术有望从根本上有效减缓MBR膜表面的生物污染。文中综述了微生物信号分子、QS机制以及各种控制MBR膜污染的QQ方法,为MBR膜生物污染控制技术的发展提供了相关信息。 相似文献
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膜生物反应器(membrane bioreactor,MBR)已成为一个十分有前景的废水处理工艺,该工艺具有出水水质好、占地面积小等优势.然而,由于微生物附着在膜表面而发生的生物污染现象,致使出水通量下降,限制了MBR的大规模推广应用.最近,一种新兴的、基于群体感应(quorum sensing,QS)的群体淬灭(quorum quenching,QQ)技术在延缓MBR膜污染领域的应用备受关注.QQ通过干扰群体感应系统而阻止其所依赖信号分子的基因表达,从而可有效抑制膜表面生物膜的形成.本文首先介绍了群体感应机理,并根据不同的群体淬灭物质,归纳总结了群体淬灭技术在延缓MBR膜污染的最新研究进展,最后对该领域的研究进行了展望. 相似文献
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膜生物反应器生物污染及其控制 总被引:1,自引:0,他引:1
综述了膜生物反应器(MBR)中生物污染的机理,以及影响生物污染的各种因素,包括水动力学条件、膜组件结构、膜材料及其性质、污泥特性、操作条件和环境条件等。并从膜模型设计、反冲洗、化学清洗和MBR系统的设计优化4个方面介绍了生物污染的控制措施。 相似文献
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微生物群体感应现象已经在污水处理中被验证并应用于膜污染控制,但是其在复合式膜生物反应器(HMBR中的膜污染控制研究鲜有报道。构建了HMBR污水处理系统,并在不同污泥龄(SRT=10、20、30 d)下运行HMBR设备,通过分析出水水质考察HMBR污水处理系统对污染物的去除效能;采用高通量测序检测群体感应相关菌群的群落结构,并结合跨膜压差、信号分子浓度深入解析群体感应调控HMBR膜污染的机理。实验结果表明,HMBR在SRT=30 d的工况下出水水质最好,膜组件运行周期最长,为最佳工况;HMBR内检测到多种群体感应猝灭(QQ)菌,其中从单胞菌科(Comamonas)、黄单胞菌科(Xanthomonadacea)和厌氧绳菌科(Anaerolineaceae)相对丰度较大;在长SRT下QQ菌比群体感应(QS)菌相对丰度更具优势,有助于降低HMBR内部信号分子的浓度,阻碍EPS分泌过程的正常进行,从而优化膜组件运行周期,因此通过延长SRT来抑制群体感应强度是一种有效的HMBR膜污染调控手段。 相似文献
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陶瓷膜因其具有耐高温高压、耐腐蚀、耐有机溶剂、膜通量高和使用寿命长等优点被广泛运用于水处理、食品饮料、医药、化工、电子以及环保等领域.但陶瓷膜的污染尤其是生物污染成为制约其实际运用的-个重要因素,因此陶瓷膜的抑菌改性越来越受到人们关注.本文论述了陶瓷膜生物污染过程及其控制方法,介绍了常见的无机抑菌材料和目前抑菌陶瓷膜的相关研究现状和趋势. 相似文献
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反渗透膜生物污染的研究进展 总被引:1,自引:0,他引:1
综述了近年来关于反渗透膜生物污染的研究进展。首先介绍了反渗透膜生物污染的机理,包括微生物与膜表面的吸附作用以及外界因素对微生物沉积的影响;然后阐述了如何监测和预警生物污染;并讨论了对生物污染的控制和预防方法,包括预处理、膜表面改性、膜清洗以及微生物群体感应抑制。 相似文献
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膜生物反应器污水处理过程中膜生物污染的研究进展 总被引:18,自引:2,他引:18
本文论述了用于污水处理的膜生物反应器的膜污染及其影响因素,同时,重点分析了膜生物污染的形成机理、微生物粘附和繁殖生长,并讨论了膜的生物污染现象、形成过程、危害以及防治措施。 相似文献
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Filomena Nazzaro Florinda Fratianni Raffaele Coppola 《International journal of molecular sciences》2013,14(6):12607-12619
Most infectious diseases are caused by bacteria, which proliferate within quorum sensing (QS)-mediated biofilms. Efforts to block QS in bacteria and disrupt biofilms have enabled the identification of bioactive molecules that are also produced by plants. This mini review primarily focuses on natural QS inhibitors, which display potential for treating bacterial infections and also enhance the safety of food supply. 相似文献
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Fang Chen Yuxin Gao Xiaoyi Chen Zhimin Yu Xianzhen Li 《International journal of molecular sciences》2013,14(9):17477-17500
With the emergence of antibiotic-resistant strains of bacteria, the available options for treating bacterial infections have become very limited, and the search for a novel general antibacterial therapy has received much greater attention. Quorum quenching can be used to control disease in a quorum sensing system by triggering the pathogenic phenotype. The interference with the quorum sensing system by the quorum quenching enzyme is a potential strategy for replacing traditional antibiotics because the quorum quenching strategy does not aim to kill the pathogen or limit cell growth but to shut down the expression of the pathogenic gene. Quorum quenching enzymes have been identified in quorum sensing and non-quorum sensing microbes, including lactonase, acylase, oxidoreductase and paraoxonase. Lactonase is widely conserved in a range of bacterial species and has variable substrate spectra. The existence of quorum quenching enzymes in the quorum sensing microbes can attenuate their quorum sensing, leading to blocking unnecessary gene expression and pathogenic phenotypes. In this review, we discuss the physiological function of quorum quenching enzymes in bacterial infection and elucidate the enzymatic protection in quorum sensing systems for host diseases and their application in resistance against microbial diseases. 相似文献
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Yunpeng Bai Santoshkumar N. Patil Steven D. Bowden Simon Poulter Jie Pan George P. C. Salmond Martin Welch Wilhelm T. S. Huck Chris Abell 《International journal of molecular sciences》2013,14(5):10570-10581
In this paper, we investigated the intra-species bacterial quorum sensing at the single cell level using a double droplet trapping system. Escherichia coli transformed to express the quorum sensing receptor protein, LasR, were encapsulated in microdroplets that were positioned adjacent to microdroplets containing the autoinducer, N-(3-oxododecanoyl)- l-homoserine lactone (OdDHL). Functional activation of the LasR protein by diffusion of the OdDHL across the droplet interface was measured by monitoring the expression of green fluorescent protein (GFP) from a LasR-dependent promoter. A threshold concentration of OdDHL was found to induce production of quorum-sensing associated GFP by E. coli. Additionally, we demonstrated that LasR-dependent activation of GFP expression was also initiated when the adjacent droplets contained single E. coli transformed with the OdDHL synthase gene, LasI, representing a simple quorum sensing circuit between two droplets. 相似文献
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Quorum quenching activity of indigenous quorum quenching bacteria and its potential application in mitigation of membrane biofouling
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Yanling Gu Jinhui Huang Guangming Zeng Yahui Shi Yi Hu Bi Tang Jianxin Zhou Weihua Xu Lixiu Shi 《Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986)》2018,93(5):1394-1400
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Celine Bergonzi Michael Schwab Tanushree Naik Prof. Dr. Mikael Elias 《Chembiochem : a European journal of chemical biology》2019,20(14):1848-1855
Quorum quenching lactonases are enzymes capable of hydrolyzing lactones, including N-acyl homoserine lactones (AHLs). AHLs are molecules known as signals in bacterial communication dubbed quorum sensing. Bacterial signal disruption by lactonases was previously reported to inhibit behavior regulated by quorum sensing, such as the expression of virulence factors and the formation of biofilms. Herein, we report the enzymatic and structural characterization of a novel lactonase representative from the metallo-β-lactamase superfamily, dubbed GcL. GcL is a broad spectrum and highly proficient lactonase, with kcat/KM values in the range of 104 to 106 m −1 s−1. Analysis of free GcL structures and in complex with AHL substrates of different acyl chain length, namely, C4-AHL and 3-oxo-C12-AHL, allowed their respective binding modes to be elucidated. Structures reveal three subsites in the binding crevice: 1) the small subsite where chemistry is performed on the lactone ring; 2) a hydrophobic ring that accommodates the amide group of AHLs and small acyl chains; and 3) the outer, hydrophilic subsite that extends to the protein surface. Unexpectedly, the absence of structural accommodation for long substrate acyl chains seems to relate to the broad substrate specificity of the enzyme. 相似文献