共查询到20条相似文献,搜索用时 765 毫秒
1.
Phosphatidylcholines and phosphatidylethanolamines were isolated from hepatoma 7288CTC, normal liver, and host liver of rats
fed one of the following diets: fat-free diet; fat-free diet supplemented with safflower oil, safflower oil fatty acids, or
partially hydrogenated safflower oil fatty acids; and commercial chow. Thecis andtrans octadecenoate fatty acids were isolated from the 1- and 2-positions of both phosphoglycerides and analyzed quantitatively
for chain positional isomers. Octadecenoates from hepatoma and liver phosphoglycerides of animals fed fat-free or natural
fatsupplemented diets contained almost exclusively twocis isomers: oleic and vaccenic acids. Oleic acid predominated in the 2-position octadecenoates of both phosphoglycerides from
hepatoma and liver. In contrast, vaccenic acid predominated in the 1-position of normal liver phosphatidylcholine and, to
a lesser extent, phosphatidylethanolamine. Host liver and hepatoma exhibited a shift to a higher percentage of oleic acid
at the 1-position. Dietarytrans fatty acids were incorporated predominately in the 1-position of both phosphoglycerides of hepatoma and liver. Except for
thecis Δ10 octadecenoate isomer, all of the unnatural dietarycis isomers between Δ8 and Δ14 were incorporated into the 1-position of the phospholipids, while the unnaturalcis octadecenoates at the 2-position consisted primarily of the Δ12 isomer. Hepatoma phosphoglycerides contained higher percentages
of thetrans Δ10 isomer that was nearly excluded from the 1-position of the two liver phosphoglycerides. All the othertrans octadecenoate isomers were incorporated into the 1-position of both phosphoglycerides, but the small amount oftrans fatty acids incorporated into the 2-position of liver and hepatoma phosphatidylcholine consisted of four isomers, Δ9 to Δ12,
including the Δ10 isomer. Phosphatidylethanolamine exhibited a similar distribution, except for the presence of the Δ13 and
Δ14 isomers at the 2-position. A combination of evidence suggests that the 1-position fatty acids in phosphatidylcholine and
phosphatidylethanolamine are of similar origin. The octadecenoates at the 2-position of these two phosphoglycerides appear
to be of the same origin in hepatoma but not in liver. It was also revealed that the 2-position of hepatoma phosphatidylcholine
contained much higher percentages of palmitate than liver. 相似文献
2.
Subcellular fractions of nuclei, mitochondria, endoplasmic reticulum, plasma membrane and cytosol were prepared from liver
and hepatoma 72288CTC. Marker enzyme activities, biochemical compositions and electron microscopy were used to establish purity.
Hepatoma NADH: cytochrome C reductase and 5′-nucleotidase exhibited abnormal subcellular distributions.
The lipids from the subcellular fractions were examined in detail. Mitochondria and plasma membranes were characterized by
elevated percentages of diphosphatidylglycrerol and sphingomyelin, respectively, in both tissues. All hepatoma subcellular
fractions contained dramatically elevated levels of sphingomyelin and cholesterol, two components that form preferential strong
complexes in vitro. The fatty acid composition of hepatoma sphingomyelin differed markedlg from liver and, unlike liver, did
not exhibit organelle specific compositions. Some hepatoma lipid classes contained reduced percentages of palmitate while
others contained higher levels. Hepatoma phosphatidylcholine and phosphatidylethanolamine from organelles contained lower
percentages of long chain polyunsaturated fatty acids than liver. Generally, unique fatty acid profiles exhibited by individual
phospholipid classes of liver subcellular fractions were absent or much reduced in the hepatoma. The ratios of oleate to vaccenate
were near one for most of the phospholipid classes of most liver fractions, but all hepatoma classes, with few exceptions,
contained a much higher percentage of oleate in all subcellular fractions. The hypothesis is proposed that the origin of some
acyl moieties for the biosynthesis of various hepatome lipid classes differs from liver sources. The possible changes in acyl
pools, sources and compartments for complex lipid biosynthesis could result in change in the quantities of molecular species
that could contribute to the abnormal properties of the hepatoma membranes. 相似文献
3.
The lipid composition of Yoshida ascites hepatoma cells was analyzed together with that of ascitic plasma and of livers and
blood plasma from host and normal rats. In comparison to normal livers, host livers showed no significant differences in the
content of the various lipid classes, but contained a higher percentage of palmitic acid and a lower proportion of arachidonic
acid in the major phospholipid classes. In addition, tumor growth induced a marked hypertriglyceridemia in host animals; changes
in the concentration of other plasma lipid classes were not statistically significant. The ascitic plasma contained small
amounts of lipids mainly constituted by cholesteryl esters and phospholipids. Yoshida hepatoma cells contained less phospholipids
in comparison to both host and normal liver, while the increased level of triglycerides and the decrease of free fatty acids
were not statistically significant. Hepatoma cells showed appreciable amounts of ether-linked lipids associated in part to
neutral lipids (as glyceryl ether diesters) and, in part, to ethanolamine and choline phosphoglycerides. The alkyl groups
in GEDE as well as in ethanolamine and choline phosphoglycerides were mainly constituted by C16∶0 and C18∶0 followed by C18∶1. The alk-1-enyl groups in ethanolamine and choline phosphoglycerides were C16∶0 and C18∶0 with only a minor proportion of C18∶1. In comparison to both host and normal liver, Yoshida hepatoma cells showed significant changes in the fatty acid composition
of neutral lipids and phospholipids. Some of the major changes consisted of an increase of monoenoic acids associated with
a decrease of arachidonic and docosahexaenoic acids in phosphatidylethanolamine, phosphatidylcholine, and phosphatidylinositol. 相似文献
4.
Groups of normal and hepatoma (7288CTC) bearing rats were maintained on normal chow and fat-free diets for 4 weeks. Normal liver, host liver, and hepatoma neutral lipids were examined in detail and compared. Water content, unaffected by diet was: hepatoma, 82 percent; host liver, 71 percent; and normal liver, 67 percent. The fat-free diet had no effect upon the hepatoma neutral lipids but elevated the triglyceride level in normal and host liver, shifted the triglyceride carbon number distribution to lower mol wt, and elevated the percentage of monoenoic acids in triglycerides and cholesteryl esters. Host triglyceride concentrations were ca. half, and cholesterol levels were reduced moderately relative to normal liver values. Hepatoma cholesterol levels were higher and triglyceride concentrations lower than normal and host liver values. Hepatoma triglycerides differed dramatically from liver and were characterized by increased concentrations of high mol wt species and a fivefold increase in the percentage of C-20 and C-22 fatty acids. The percentage of C-20 and C-22 fatty acids in hepatoma cholesteryl esters also increased ca. fivefold relative to liver. The data indicate that the systems that regulate triglyceride and cholesteryl ester fatty acid composition in liver do not control the compositions of these lipid classes in this hepatoma. The unchanged high level of essential fatty acids in the hepatoma lipids from the fat-free fed animals demonstrates the hepatoma's ability to absorb and conserve specific fatty acids. 相似文献
5.
Monoenoic acid fractions were isolated from phosphatidycholine, phosphatidylethanolamine, triglycerides, and cholesteryl esters of hepatoma 7288CTC, host liver, and normal liver from animals maintained on chow and fat free diets. Hexadecanoate (16:1), octadecenoate (18:1), and eisosenoate (20:1) fractions were analyzed quantitatively for their isomeric composition. The fat free diet had little or no effect relative to the chow diet on the isomeric composition of 16:1, 18:1, and 20:1 from any lipid class in either heptoma, host liver, or normal liver. Host livers were reduced in palmitoleic acid, and oleic and eicos-11-enoic acids were increased relative to normal liver. The 16:1 fraction from triglyceride of normal liver, host liver, and hepatoma contained 90, 80, and 75% palmitoleic acid, respectively. The 20:1 fraction from triglycerides of normal liver, host liver, and hepatoma contained ca. 55, 70, and 60% eicos-11-enoic acid, respectively, with the remainder consisting of eicos-13-enoic acid. The proportion of vaccenic acid in the 18:1 fraction was 60, 50, 20, and 25% for phosphatidylethanolamine, phosphatidylcholine, triglycerides, and cholesteryl esters, respectively, with oleic acid making up the balance. In contrast, all hepatoma lipid classes exhibited the same proportion of oleic (70%) and vaccenic (30%) acids. These data appear to be the first to demonstrate lipid class specificity for isomeric octadecenoic acids in normal liver and the loss of this specificity in a neoplasm. 相似文献
6.
The phospholipids from the livers of adult pigeons were separated by thin layer chromatography and the component fatty acids
analyzed by gas liquid chromatography. They consisted of 53.0% phosphatidylcholine, 26.3% phosphatidylethanolamine, 8.6% sphingomyelin,
6.3% cardiolipin and 4.8% lysophosphatidylcholine. Phosphatidylethanolamine and phosphatidylcholine were characterized by
a high concentration of long-chain polyunsaturated fatty acids with the highest percentages in the phosphatidylethanolamine.
Sphingomyelin contained up to 64.5% saturated acids. About 80% of the fatty acids present in the cardiolipin fraction consisted
of linoleic acid. The liver phospholipids had the same composition in lactating as in nonlactating pigeons, but differed in
many respects from those available in the crop-milk. 相似文献
7.
Minimal deviation hepatoma cells were cultured in a modified Swim's 77 medium supplemented with decreasing amounts of serum,
lipid-free serum, and lipid-free serum containing added palmitic or linoleic acids. Cellular phospholipids were extracted
and the class distribution determined quantitatively. The fatty acid composition of each phospholipid class was determined,
and the percentages from cells grown on each of the various media were compared. Cellular phospholipid class and fatty acid
compositions differed from media compositions, indicating that intact serum phospholipids are not incorporated into cellular
structures. Phosphatidylcholine percentages decreased as the media serum and lipid levels decreased, while phosphatidylinositol
and phosphatidylethanolamine percentages increased. Sphingomyelin of cells grown in medium containing added linoleic acids
contained a high level of a 24∶2 acid. All classes, except sphingomyelin, contained elevated levels of 18∶1 acid and decreased
levels of polyunsaturated fatty acids, relative to normal rat liver. Cells cultured on lipid-free medium did not contain increased
concentrations of 20∶3 acid, suggesting that this hepatoma cell cannot desaturate monoenoic acids. Phosphoglycerides of cells,
grown on lipid-free medium, had the highest monoene fatty acid concentration, whereas those cells grown on media containing
added linoleic acid had the lowest concentrations, suggesting that linoleate may inhibit or regulate monoenoic acid biosynthesis
in this cell. These mass data also demonstrate that monoenoic fatty acid biosynthesis in this cultured hepatoma cell responds
to dietary changes. 相似文献
8.
Randall Wood 《Lipids》1975,10(7):404-408
Triglycerides from normal liver, host liver, and hepatoma of rats maintained on chow and fat-free diets were subjected to
stereospecific analysis. Normal and host liver triglycerides from animals on the same diet did not exhibit significant differences.
Fat-free diet reduced polyunsaturated fatty acids in normal and host liver triglycerides, but had no effect upon hepatoma
triglycerides. Each position of hepatoma and liver triglyceride glycerol exhibited a characteristic fatty acid composition.
Palmitate concentrations were reduced dramatically and stearate levels were increased significantly at the 1 position of hepatoma
triglycerides, relative to the corresponding position of liver triglycerides which were affected little by diet or tumor.
Except for higher percentages of C-20 and higher fatty acids, common to all three positions, the composition of hepatoma triglycerides
at the 2 position appeared normal. The 3 position of hepatoma triglycerides contained significantly higher percentages of
stearate than liver. Data obtained previously for Ehrlich ascites cell triglycerides were in good agreement with this hepatoma.
Data from these two neoplasms suggest that the metabolic system that regulates or controls the fatty acid composition at the
1 and 3 positions of normal tissue triglycerides does not function normally in neoplasms. 相似文献
9.
The lipid composition of Morris hepatoma 5123c was analyzed together with that of liver and blood plasma from both normal
and tumor-bearing rats. The results showed that the liver of tumor-bearing rats contained higher amounts of glycerides, cholesteryl
esters, free fatty acids and phospholipids than the liver of normal rats. In the blood plasma of tumor-bearing rats, there
was an increase of free cholesterol and triglycerides; this latter difference, however, was not statistically significant.
Acyl chain changes in the liver of tumor-bearing rats consisted of an increase of palmitic and oleic acids and a decrease
of stearic and arachidonic acids in phosphatidylinositol. Morris hepatoma 5123c contained a lower amount of triglycerides
than the livers (both host and normal) and showed a significant decrease of total phospholipids when compared to the host
liver. The major acyl chain changes found in Morris hepatoma 5123c compared with both normal and host rat livers were: a)
a higher percentage of arachidonic acid together with a lower proportion of palmitic acid in cholesteryl esters; b) an increase
of stearic and arachidonic acids and a decrease of palmitic acid in triglycerides; and c) a higher level of palmitic and oleic
acids associated with a lower percentage of stearic and C22 polyunsaturated acids in phosphatidylcholine. 相似文献
10.
J. E. Kinsella 《Lipids》1972,7(3):165-170
The microsomes isolated from lactating bovine mammary tissue contained 4.3 mg lipid per milligram nitrogen. Phospholipids
comprised 83% of the lipids. The neutral lipids were composed of triglycerides (20–30%), diglycerides (5–10%), free fatty
acids (15–30%, cholesterol (35–40% and cholesterol esters (10–12%, respectively. Phosphatidylcholine was the predominant phospholipid
component (>50%), and the remainder consisted of phosphatidylethanolamine (21–13%), phosphatidylserine (4–6%), phosphatidylinositol
(8%), sphingomyelin (9%) and lysophosphatidylcholine (2%) respectively. The composition of the microsomal phospholipids was
similar to that of isolated mammary cells and tissue homogenates but quite different from milk and fat globule membrane phospholipids.
The triglycerides contained short chain fatty acids but their relative concentrations were lower than in milk triglycerides.
The various lipid fractions had a variable proportion of saturated fatty acids, i.e., triglycerides (47.7%), diglycerides
(86.7%), free fatty acids (70.6%), phosphatidylcholine (50.6%), phosphatidylethanolamine (50.8%), phosphatidylserine (35.3%),
phosphatidylinositol (40.5%) and sphingomyelin (82.3%), respectively. The molecular distribution of fatty acids in the microsomal
triglycerides and phosphatidylcholine was similar to that occurring in milk, i.e., the short chain and unsaturated fatty acids
were concentrated in the primary positions (sn1 andsn3) of the triglycerides, and the unsaturated acids were preferentially located in positionsn2 of the phosphatidylcholine. The compositional data indicate that mammary microsomes are not the direct source of the phospholipids
of the milk fat globule. 相似文献
11.
Silicic acid column chromatography was used to separate the polar lipids of goats' milk into glycolipid, phosphatidylethanolamine,
phosphatidylserine plus phosphatidylinositol, phosphatidylcholine, and sphingomyelin fractions. Each fraction was purified
by column chromatography and its fatty acid profile determined by gas liquid chromatography and mass spectrometry. The glycerophospholipids
each contained 18∶1 as the predominant fatty acid (∼45%). The sphingolipids contained a high percentage of long-chain saturated
fatty acids (C22 to C24>45%); the glycolipid fraction also contained ca. 2% 2-hydroxy fatty acids. The data represent a comprehensive cross-sectional
study of the major polar lipids found in goats' milks. 相似文献
12.
Minimal deviation hepatoma 7288C cells (HTC) were incubated in serum-supplemented and serum-free Swim’s 77 medium in the presence
of D-[1-14C] glucose for 1, 2, 4, 8, 12 and 24 hr. Glucose oxidation to CO2, incorporation into total cell mass, and incorporation into cell and medium lipids were determined. The percentage distribution
of total cell lipid radioactivity in individual neutral and polar lipid classes was followed as a function of time. Degradation
studies of individual lipid classes were performed to ascertain the percentage of radioactivity in acyl and glycerol moieties.
The percentage of D-[1-14C] glucose oxidized to14CO2, incorporated into cell matter and cell lipids was elevated in cells incubated in serum-free medium as opposed to serum-supplemented
medium. The percentage distribution of total cell lipid radioactivity into individual neutral lipid classes from both serum-free
and serum-supplemented cultures was as follows: sterols > triglycerides > free fatty acids > sterol esters. The percentage
distribution of total cell lipid radioactivity into individual polar lipid classes of serum-supplemented cultures was as follows:
phosphatidylcholine > phosphatidylinositol > sphingomyelin > phosphatidylethanolamine > phosphatidylserine. The distribution
of glucose radiolabel into individual polar lipid classes of serum-free HTC cells was different from their serum-supplemented
counterparts: sphingomyelin > phosphatidylcholine > phosphatidylinositol > phosphatidylethanolamine > phosphatidylserine.
Glycerol from glyceride classes contained a higher percentage of radioactivity than the acyl moieties, with this percentage
significantly elevated in serum-free cultures. The data indicate that, although glucose is a substrate for HTC cell lipids,
other precursors present in the culture system also contribute to the lipid constituency of this hepatoma cell line. 相似文献
13.
The lipid composition of human liver microsomes isolated from liver biopsy samples obtained at abdominal surgery has been
determined. Human liver microsomal phospholipid is composed of 49% phosphatidylcholine, 31% phosphatidylethanolamine, 14%
phosphatidylserine+phosphatidylinositol and 6% sphingomyelin, very similar to the phospholipid composition of rat liver microsomes.
The fatty acid composition of human liver microsomes is remarkable only for its content of polyunsaturated fatty acids, with
20% of the fatty acids consisting of arachidonic, docosatetraenoic, docosapentaenoic and docosahexaenoic acids. This value
contrasts with 33% in rats and 9% in rabbits. The molar cholesterol/phospholipid ratio in human liver microsomes is 0.069,
similar to the ratio in rat and rabbit microsomes. 相似文献
14.
L. J. Filer Jr. Samuel J. Fomon Thomas A. Anderson Thomas L. Nixt Dean W. Andersen 《Lipids》1974,9(8):536-540
Fatty acid composition of carcass and liver and proximate analysis of liver were studied in 14–28 day old Pitman-Moore miniature pigs, 26 sow-reared and 30 fed a semisynthetic diet in which the fat was lard. With increasing age, fat of carcass, but not of liver, became significantly more unsaturated. The percentage of palmitic acid (16∶0) and total saturated fatty acids was significantly greater and the percentage of linoleic acid (18∶2) and total unsaturated fatty acids significantly less in carcasses of male than of female pigs. No sex-related differences in proximate or fatty acid composition of the liver were noted. Carcasses of sow-reared pigs contained significantly greater percentages of myristic (14∶0), palmitoleic (16∶1), and linoleic acids and significantly lesser percentages of stearic (18∶0) and oleic (18∶1) acids than did those of pigs fed the semisynthetic diet. Diet-related differences in fatty acid composition of liver closely paralleled those of carcass, although liver contained markedly greater percentages of stearic and arachidonic (20∶4) acids and lesser percentages of palmitoleic and oleic acids than did carcass. Diet-related differences in fatty acid composition of carcass and liver are discussed in relation to the fatty acid composition of dietary fat (sow milk and lard). 相似文献
15.
The effect of dietary cyclopropene fatty acids on the concentration of octadecenoate chain positional isomers in individual
lipid classes of normal liver, host liver, and hepatoma 7288CTC has been determined. The data revealed the following: (a)
Saturated and monoene fatty acid percentages of liver phosphatidylcholines and phosphatidylethanolamines werenot affected, but the percentage of saturated fatty acids of the triglycerides and cholesteryl esters was increased while the
monoene percentages decreased. (b) Oleate to vaccenate percentage ratios, previously shown to be characteristic of individual
lipid classes, were completely disrupted. (c) Oleate concentrations of the two major liver phospholipids were elevated, and
vaccenate levels were dramatically reduced. (d) Cyclopropene fatty acids appear to inhibit monoene elongation. (e) The elevated
concentrations of oleate indicate that an alternate route of oleate biosynthesis must exist if the Δ9 desaturation is inhibited
by cyclopropene fatty acids as reported previously. (f) In contrast to liver, oleate and vaccenate concentrations in hepatoma
were not affected by the dietary cyclopropene fatty acids. 相似文献
16.
This work describes a one-step separation of rat tissue phospholipid classes by high-performance liquid chromatography (HPLC)
using a silica column and a new light-scattering detector (LSD). Complete separation of phosphatidylcholine, phosphatidylethanolamine,
diphosphatidylglycerol, phosphatidylinositol, phosphatidylserine, sphingomyelin, lysophosphatidylethanolamine, and lysophosphatidylcholine
was obtained. Direct quantification was achieved after detector calibration for each phospholipid class. The detector response
was shown to be linear within the ranges used. The LSD results agreed well with those obtained by phospholipid phosphorus
assay. The present method was applied to rat heart and rat liver phospholipid analysis. 相似文献
17.
Phosphatidylcholine. phosphatidylethanolamine, and triglycerides were isolated from minimal deviation hepatoma 7288C cells cultured as monolayers to confluency in roller flasks containing Swim's 77 medium supplemented with 5% fetal calf serum, plus 20%, 10%, or 5% bovine serum. Fatty acid distribution at each position of glycerol was determined for the 3 glycerolipid classes, and carbon number distributions of triglycerides and diglycerides derived from phosphatidylcholine and phosphatidylethanolamine were quantitated by high temperature gas liquid chromatography. Fatty acid composition was only marginally affected by the level of bovine serum in the culture medium. Percentage composition of fatty acids esterified at each position of the 3 glycerolipids was different, indicating a nonrandom distribution of acyl groups in triglycerides and the 2 diacyl phosphatides. The carbon number distribution of diglycerides derived from phosphatidylcholine and phosphatidylethanolamine was different, and neither carbon number distribution agreed with the calculated 1-random, 2-random diacyl distribution, thus indicating pairing of certain acids in the diglycerides derived from these phospholipd classes. The determined triglyceride carbon number distributions did not show complete agreement with those calculated, assuming a 1-random, 2-random, 3-random type of fatty acyl distribution, suggesting preferential pairing of some acids in this lipid class. The 1-, 2-diglycerides derived from phosphatidylcholine, phosphatidylethanolamine, and triglycerides differed, indicating either selectivity in utilization of diglyceride species in biosynthesis of these glycerolipids, or modification of glycerolipids after their initial synthesis. 相似文献
18.
To shed light on the question whether the phospholipids present in chromatin are native or are due to contamination from nuclear
membranes, we labeled the phospholipids of isolated nuclei and determined the amount of phospholipids (PL) and PL fatty acid
composition in nuclei and chromatin. The hepatocyte nuclei were isolated and radioiodinated by the lactoperoxidase method
under saturating and nonsaturating conditions, and the radioactivity associated with chromatin extracted from these nuclei
was monitored. Whereas 97% the label was recovered in the nuclear membranes, only 0.08–0.6% was found in chromatin. The PL
present in chromatin were relative to the amounts present in the entire nuclei and calculated as percentage of total, phosphatidylethanolamine
(10%), phosphatidylserine (22%), phosphatidylinositol (19%) phosphatidylcholine (14%), and sphingomyelin (35%). In sphingomyelin
of chromatin-associated PL an enrichment in polyunsaturated fatty acids was seen. The data indicated that the PL found in
isolated chromatin do not seem to be due to contamination from the nuclear membrane. 相似文献
19.
U. Stoll 《European Journal of Lipid Science and Technology》1995,97(6):229-235
The Characterization of Diglycerides from Phospholipids of Cardiac Muscle from Guinea Pig The phospholipid composition of mitochondria and microsomes from cardiac muscle of guinea pig was characterized. Diglycerides of phosphatidylcholine and phosphatidylethanolamine were prepared by reaction with phospholipase C. The separation of molecular species of both phospholipids via diglyceride acetate was achieved on kieselgel plates impregnated with silver nitrate. The diglycerides were characterized by their fatty acid pattern. Diacyl- and alk-1-enyl-acyl-species with identical fatty acids in sn2-position could be separated from each other. In plasmalogens (= alk-1-enyl-acyl form) the saturated fatty acids in sn1-position of diacylphospholipids are substituted by a vinyl ether of the same chain length as the fatty acid. The diacyl- as well as the alk-1-enyl-acyl composition was rather typical for cholineglycerophospholipids and for ethanolamineglycerophospholipids. 相似文献
20.
Anette Bysted Gunhild Hølmer Pia Lund 《Journal of the American Oil Chemists' Society》1998,75(2):225-234
The effect of trans fatty acids from partially hydrogenated soybean oil and butterfat on the formation of polyunsaturated fatty acids was investigated.
Five groups of rats were fed diets that contained 20 wt% fat. The content of linoleic acid was adjusted to 10 wt% of the dietary
fats in all diets, whereas the amount of trans fatty acids from partially hydrogenated soybean oil (PHSBO) was varied from 4.5 to 15 wt% in three of the five diets. The
fourth group received trans fatty acids from butterfat (BF), while the control group was fed palm oil without trans fatty acids. Trans fatty acids in the diet were portionally reflected in rat liver and heart phosphatidylethanolamine (PE), phosphatidylcholine
(PC), phosphatidylinositol, and phosphatidylserine. Incorporation in the sn-1 position was compensated by a decrease in saturated fatty acids. Trans fatty acids were not detected in diphosphatidylglycerol. Compared to the presence in the dietary fats, 8t- and 10t-18:1 were discriminated against in the incorporation in PE and PC from liver and heart, whereas 9t- and 12t-18:1 were preferred. The formation of 20:4n-6 was not influenced by 4.5 wt% trans fatty acids (from PHSBO) but apparently was by 10 wt% in liver. In contrast, even a content of 2.5 wt% trans fatty acids from BF reduced the formation of 20:4n-6. The inhibitory effect of trans isomers on linoleic acid conversion was reflected less in heart than in liver and less for PE than for PC. Groups with trans fatty acids showed increased 22:6n-3 and 22:5n-3 deposition in liver and heart PE and PC. 相似文献