气调包装中气体浓度对牛肉保鲜的影响

研究了气调包装中不同气体浓度对牛肉保鲜效果的影响。运用均匀设计法,对新鲜牛肉分别进行不同水平的气调保鲜包装(O2与CO2浓度在0%～100%均匀分布),在(4±1)℃贮藏过程中,隔天对包装内的气体浓度、牛肉的菌落总数、pH值、TVB-N值、液汁流失率、红度值等指标进行测定分析,并采用重回归分析法找出气调包装中O2浓度、CO2浓度、贮藏时间与菌落总数之间的关系式。结果表明:高氧或高CO2浓度的气调包装对牛肉保鲜效果都不理想,而适当的气体组合45%O2/30%CO2/25%N2的气调包装牛肉货架期最长。     

牛肉冰温气调保鲜技术的研究

研究了以不同组合比例的CO2、O2、N2的气调包装,结合冰温（-1．5±1℃）贮藏的牛肉保鲜技术。以空气包装为对照,以牛肉的色差值、菌落总数、失水率、挥发性盐基氮、PH值为衡量指标,综合评价冰温气调保鲜牛肉的效果。研究结果表明,最佳的气调包装组分为10％O2,70％CO2和20％N2,在此条件下可以保鲜达28天;保鲜牛肉的PH值为6．09,挥发性盐基氮值为14．634mg／100g,菌落总数为3．7×10^4efu／g,色泽紫红,无异味。     

气调包装对牛肉保鲜效果的影响

冷却牛肉的嫩化及保鲜问题在一定程度上制约了冷却牛肉的普及和发展,气调包装是一种通过调节和控制食品所处环境中气体组成的保鲜方法,并在一定时间内保持相对稳定,以抑制或延缓产品的变质过程,从而达到延长保鲜期和提高保鲜效果的目的。本实验以牛肉为包装对象,通过研究5种不同透气性包装材料在气调包装(45%氧气+30%二氧化碳+25%氮气)气体比例的充气包装方式下,每天对牛肉菌落总数、酸碱值、挥发性盐基氮值、色泽、包装袋内气体含量的变化和剪切力进行测定分析。结果表明:以聚丙烯-聚乙烯-聚对苯二甲酸乙二醇酯的气调包装材料效果最佳。     

牛肉气调保鲜包装研究进展

气调包装(MAP)技术应用于牛肉保鲜是当前食品包装领域的研究热点之一。本文从MAP对牛肉品质的影响、牛肉MAP保鲜效果的影响因素、MAP与其他保鲜方法协同作用等方面进行了阐述,可为牛肉保鲜包装中包装技术及材料选择提供指导。     

不同保鲜剂对蓝莓保鲜效果的比较

为了延长蓝莓的贮藏期,本研究以蓝莓为原料,研究了丁香酚缓释保鲜、丁香提取液、乙醇熏蒸、气调保鲜、丁香酚缓释结合气调保鲜5种不同保鲜剂对其贮藏期品质的影响。结果显示,这5种保鲜剂能显著抑制蓝莓果实腐烂和质量损失率,有效缓解可溶性固形物、可滴定酸、还原糖、Vc含量的降低,抑制MDA含量的上升,同时,可使蓝莓果实保持较高的SOD活力。贮藏49 d时,经丁香酚缓释结合气调包装的蓝莓腐烂率和失重率分别为50.13%和2.71%,可溶性固形物、可滴定酸、还原糖、Vc、花色苷含量分别为7.06%、7.26 g/kg、4.14 g/100 g、0.93 g/100 g和117.48 mg/100 g,MDA含量为26.84 nmol/g,SOD活力为176.34 U/mL。与对照组相比,丁香酚缓释结合气调包装能延长蓝莓14 d贮藏期。结果表明,5种保鲜处理对蓝莓具有一定的保鲜作用,其中丁香酚缓释结合气调包装保鲜方式最好。     

气调包装对大菱鲆的冷藏保鲜效果

以大菱鲆为研究对象,通过不同气调包装（60% CO2＋5% O2＋35% N2、60% CO2＋30% O2＋10% N2、40% CO2＋30% O2＋30% N2）进行处理,测定其感官、菌落总数、pH值、挥发性盐基氮（TVB-N）值、硫代巴比妥酸（TBA）值、汁液流失率等指标变化,综合评价气调包装对冷藏大菱鲆的保鲜效果。结果表明：空气包装组大菱鲆冷藏货架期为4 d,60% CO2＋5% O2＋35% N2气调包装组的保鲜效果最佳,货架期约为8 d;气调包装中高体积分数的CO2抑菌效果较为明显,能有效延缓pH值、菌落总数与TVB-N值的升高,但较高含量的CO2会导致鱼肉汁液流失较为严重;在鱼肉脂肪氧化方面,O2较CO2作用更大,高含量的O2对脂肪氧化具有一定促进作用;60% CO2＋5% O2＋35% N2气调包装组冷藏贮存8 d,大菱鲆的菌落总数为6.69（lg（CFU/g））,TVB-N值为11.99 mg/100 g,TBA值为2.34 mg/100 g。总体结果表明,气调包装能显著延长冷藏大菱鲆货架期,具有良好的保鲜抑菌作用。     

MAP结合真空预处理延长鲜牛肉货架期

通过采用真空预处理后进行气调包装技术，对生鲜牛肉冷藏过程中一系列品质的变化进行了研究，找到了延长牛肉货架期的较佳条件．主要考察了牛肉在不同的预处理终温、预处理压力及综合优化条件下保鲜效果的优劣．结果表明，选择预处理终温为4℃，预处理压力为1200Pa，联合气调包装充入体积分数40％的O2，25％的CO2气体时，保鲜效果较佳，可使牛肉货架期延长至14d以上．     

新鲜菠菜的纳米活性气调包装研究

使用自行研制的纳米活性气调包装膜,选用新鲜采摘的菠菜,进行气调包装研究.测定保鲜膜的拉伸强度、透气率和透湿率,对菠菜包装的保鲜效果进行感官评价,研究随着分子筛含量的增加,菠菜失重率、可溶性固形物和呼吸强度的变化.结果表明:在室温下,Hβ-7保鲜膜的保鲜效果最好,气调保鲜包装的平衡气体浓度为O2含量13.4%～13.7%,CO2含量3.6%～4.2%.保鲜期可长达5 d;在保鲜柜中,Hβ-7保鲜膜的保鲜效果最好,气调保鲜包装的平衡气体浓度为O2含量19.8%～20.2%,CO2含量1.2%～1.4%,货架寿命可达10 d以上.     

聚乳酸薄膜包装袋对杨桃保鲜效果的研究

杨桃分别用聚乳酸(PLA)和聚乙烯(PE)包装袋包装,包装方式采用气调包装和真空方式两种,处理好后将样品置于20℃,相对湿度95%的环境中贮藏,以未包装杨桃为对照,测定了杨桃贮藏过程中包装袋中O2和CO2含量,失重率、褐变度、可滴定酸含量、pH、可溶性固形物、多酚氧化酶(PPO)活性、抗坏血酸等变化,研究PLA薄膜包装袋包装杨桃的保鲜效果。结果表明,在真空包装对照组中,各项数据差别很小,但其对杨桃的保鲜效果普遍优于气调包装。在气调包装对照组中,气相色谱分析显示PLA袋中CO2体积分数比PE高,O2体积分数低。11 d后,PLA气调包装袋中杨桃褐变度和PPO活性为11.4%和2.3 U/min.g,低于PE气调包装的55.2%和2.9 U/min.g;有机酸分解为0.14%,pH为4.74,酸值高于PE气调包装;同时,PLA包装中杨桃的可溶性固体物和V_C含量均高于PE包装袋中杨桃。由此说明,PLA包装确实可以有效延长杨桃的货架期。     

复合保鲜剂结合MAP对鲜切茄子贮藏品质的影响

为延长鲜切茄子的保鲜时间,研究单一气调包装(2%CO2+5%O2+93%N2)和保鲜液(1.5%壳聚糖+1%抗坏血酸+1%氯化钙+2%柠檬酸)结合气调包装对鲜切茄子品质的影响。以感官评分、褐变度、硬度、多酚氧化酶(PPO)活性和细菌总数为指标,分析鲜切茄子于(10±1)℃下贮藏8d内的品质变化。结果表明:贮藏8d后,对照组样品的L*值、硬度、PPO酶活性和细菌总数分别为75.66、7.41kg/cm2、68.77U/(min·g)和5.52lg CFU/g,而经保鲜液结合气调包装的鲜切茄子各指标分别为82.51、10.05kg/cm2、23.70U/(min·g)和2.87lg CFU/g。气调包装能够抑制鲜切茄子的褐变、萎蔫等感官品质的下降,并抑制PPO活性的增加和细菌的生长繁殖,尤其在结合保鲜液处理后效果更加明显,贮藏8d仍保持较好的品质。     

Determination of ethanol,volatile fatty acids,lactic and succinic acids in fermentation liquids by gas chromatography

A rapid and simple quantitative method was developed to determine, by gas chromatography, the concentrations in fermentation liquids of ethanol, the C₂-C₆ volatile fatty acids, and lactic and succinic acids. Aqueous samples were acidified with 250μlml^?1 metaphosphoric acid (5:1 ratio), centrifuged, and injected directly on to a column containing a porous aromatic polymer (Chromosorb 101) maintained at 200°C in a gas chromatograph fitted with a flame ionisation detector. It was unnecessary to purify samples further before injection, although distillation and ion-exchange methods were examined. Derivatisation of lactic and succinic acids before injection was not necessary, but the lowest level of detection of these two relatively non-volatile acids was about four times greater than that for the volatile fatty acids. The method described was suitable for the analysis of rumen fluid, methane digester fluid, silage extracts and other anaerobic fermentation fluids. The relative retention times are given for 23 organic acids and six other fermentation end-products.     

Effects of abomasal infusions of fatty acids and 1-carbon donors on apparent fatty acid digestibility and incorporation into milk fat in cows

Our primary objective was to determine the effects of the abomasal infusion of 16-carbon (16C) and 22-carbon (22C) fatty acids (FA) on apparent FA digestibility, plasma FA concentrations, and their incorporation into milk fat in cows. Our secondary objective was to study the effects of 1-carbon donors choline and l-serine on these variables. Five rumen-cannulated Holstein cows (214 ± 4.9 d in milk; 3.2 ± 1.1 parity) were enrolled in a 5 × 5 Latin square experiment with experimental periods lasting 6 d. Abomasal infusates consisted of (1) palmitic acid (PA; 98% 16:0 of total fat), (2) PA + choline chloride (PA+CC; 50 g/d of choline chloride), (3) PA + l-serine (PA+S; 170 g/d of l-serine), (4) behenic acid (BA; 92% 22:0 of total fat), and (5) docosahexaenoic acid algal oil (DHA; 47.5% DHA of total fat). Emulsions were formulated to provide 301 g/d of total FA and were balanced to provide a minimum of 40 and 19 g/d of 16:0 and glycerol, respectively, to match the content found in the infused algal oil. Apparent digestibility of FA was highest in DHA, intermediate in PA, and lowest in BA. Digestibility of 16C FA was lowest in BA and highest in PA. The digestibility of 22C FA was highest in DHA relative to BA (99 vs. 58%), whereas 1-carbon donors had no effect on 22C FA digestibility. Plasma 16C FA concentrations were greatest with PA treatment, and 22C FA concentrations were ~3-fold greater in DHA-treated cows relative to all other treatments. Milk fat 16:0 content was highest in PA relative to BA and DHA (e.g., 37 vs. 27% in PA and DHA), whereas the milk yield of 16:0 was higher in PA relative to DHA (i.e., 454 vs. 235 g/d). Similarly, milk 22:0 content and yield were ~10-fold higher in BA relative to all other treatments, whereas DHA treatment resulted in higher content and yield of 22:6 in milk fat relative to all other treatments (41- and 38-fold higher, respectively). Consequently, the content of FA >16C (i.e., preformed) was higher in milk fat from cows infused with BA and DHA relative to PA. De novo FA content in milk did not differ between PA, PA+CC, and PA+S (~16% of milk fat) but was higher in BA and DHA treatments (19 and 21%, respectively). We conclude that FA carbon chain length and degree of saturation affected FA digestibility and availability for absorption as well as their incorporation into milk fat. The abomasal infusion of choline chloride and l-serine did not modify these variables relative to infusing palmitic acid alone.     

Short communication: Eicosatrienoic acid and docosatrienoic acid do not promote vaccenic acid accumulation in mixed ruminal cultures

Previous research found that docosahexaenoic acid (C22:6n-3) was a component of fish oil that promotes trans-C18:1 accumulation in ruminal cultures when incubated with linoleic acid. The objective of this study was to determine if eicosatrienoic acid (C20:3n-3) and docosatrienoic acid (C22:3n-3), n-3 fatty acids in fish oil, promote accumulation of trans-C18:1, vaccenic acid (VA) in particular, using cultures of mixed ruminal microorganisms. Treatments consisted of control, control plus 5 mg of C20:3n-3 (ETA), control plus 5 mg of C22:3n-3 (DTA), control plus 15 mg of linoleic acid (LA), control plus 5 mg of C20:3n-3 and 15 mg of linoleic acid (ETALA), and control plus 5 mg of C22:3n-3 and 15 mg of linoleic acid (DTALA). Treatments were incubated in triplicate in 125-mL flasks, and 5 mL of culture contents was taken at 0 and 24 h for fatty acid analysis by gas-liquid chromatography. After 24 h of incubation, the concentrations of trans-C18:1 (0.87, 0.88, and 0.99 mg/culture), and VA (0.52, 0.56, and 0.62 mg/culture) were similar for the control, ETA, and DTA cultures, respectively. The concentrations of trans-C18:1 (5.51, 5.41, and 5.36 mg/culture), and VA (4.78, 4.62, and 4.59 mg/culture) were also similar between LA, ETALA, and DTALA cultures, respectively. These data suggest that C20:3n-3 and C22:3n-3 are not the active components in fish oil that promote VA accumulation when incubated with linoleic acid.     

奶粉脂肪酸与乳制品风味关系研究

用气质（GC—MS）联用色谱分析了11个商业奶粉样品的脂肪酸组成以及含量,每个样品均检测到了28种脂肪酸,在表现奶粉风味的4个呈味脂肪酸,也即辛酸、己酸、壬酸和葵酸中只检测到了辛酸和葵酸。辛酸和葵酸含量在进口奶粉中普遍高于国产奶粉。国产奶粉中辛酸和葵酸的含量以2号最好,3号其次。亚油酸含量在国产奶粉中普遍高于进口奶粉。     

气相色谱法同时快速测定食品中丙酸、山梨酸、苯甲酸及脱氢乙酸

通过液液萃取净化样品研究,建立了食品中丙酸、山梨酸、苯甲酸、脱氢乙酸及其盐含量气相色谱同时快速测定方法,适用于固体非酯(脂)类食品的检测。结果表明:丙酸的回收率在85.1%~91.3%之间,其余3种防腐剂的回收率均在95.2%~99.4%之间;实验室内变异系数(CV,n=6)最大值≤4.7%,4种防腐剂检出限均在0.002 g/kg以下。4种目标物在有杂质干扰时,可用不同的极性毛细管柱做进一步的确认。本方法具有适用范围广、检测效率高、重现性好、准确度高、检出限低的特点,推广应用对我国食品安全的监督检验具有重要的意义。     

花生四烯酸、二十二碳六烯酸和二十碳五烯酸 在炎症中的作用概述

心脑血管疾病、肿瘤、糖尿病、神经系统疾病、自身免疫等疾病严重危害着人类的生命和健康,并消耗着大量医疗资源。事实上,很多疾病发生和发展的背后都伴随着炎症反应,炎症是众多疾病的病理基础,甚至是导致这些疾病的诱因。炎症本身是机体的防御性反应,但过度的炎症反应和长期慢性炎症会损害机体的稳态。炎症的调节和控制由炎症介质介导,花生四烯酸(arachidonic acid,AA)、二十二碳六烯酸(docosahexaenoic acid,DHA)和二十碳五烯酸(eicosapentaenoic acid,EPA)等长链多不饱和脂肪酸(10ng-chain polyunsaturated fatty acids,LC-PUFAs)的衍生物是一类重要的调控炎症的介质。炎性细胞间的交流和细胞内信号传递与LC-PUFAs有关。AA经环氧酶和脂氧合酶合成的类二十烷酸主要起促炎作用,但有的也有抗炎作用。DHA和EPA在体内起抗炎作用,由它们合成的消退素(resolvins,Rvs)和保护素(protectin,PD)是重要的抗炎活性物质。DHA和EPA还可以干扰炎性细胞内信号传导途径来抑制炎症反应。本文从炎症与疾病的关系、LC-PUFAs的衍生物及其促炎和抗炎机制等方面综述了AA、DHA和EPA在炎症中的作用。     

Growth of Aeromonas hydrophila K144 as Affected by Organic Acids

The influence of different acids on the aerobic growth kinetics of Aeromonas hydrophila was studied in BHI broth with 0.5 and 2.0% NaCl incubated at 5 and 19°C. Growth curve data were analyzed by the Gompertz equation and a nonlinear regression program; generation and lag times were calculated from the Gompertz parameters. Type of acid, pH, NaCl level and temperature influenced lag and generation times. The organic acids (acetic, lactic, citric and tartaric) inhibited growth at higher pH values than inorganic acids (HCl and H₂SO₄). The high NaCl level interacted with type of acid and pH to restrict growth of the organism at the lower temperature of incubation. Acetic and lactic acids were effective in controlling the growth of A. hydrophila and could readily be combined with low holding temperature to render foods free of the organism.     

酸味酿造产品中三酸比例评析

酸味酿造产品中乳酸、醋酸、丁酸共存,但比例不同形成的酸味特征也不同。控制不同的环境条件,创造出不同的微生物区系,形成不同的三酸比例,才能形成不同的产品风格。该文对常见的酸味酿造产品中微生物区系的变化及三酸含量进行了分析。     

Anti-obesity effects of conjugated linoleic acid, docosahexaenoic acid, and eicosapentaenoic acid

Obesity has become a prevailing epidemic throughout the globe. Effective therapies for obesity become attracting. Food components with beneficial effects on "weight loss" have caught increasing attentions. Conjugated linoleic acid (CLA), docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA) belong to different families of polyunsaturated fatty acids (PUFA). However, they have similar effects on alleviating obesity and/or preventing from obesity. They influence the balance between energy intake and expenditure; and reduce body weight and/or fat deposition in animal models, but show little effect in healthy human subjects. They inhibit key enzymes responsible for lipid synthesis, such as fatty acid synthase and stearoyl-CoA desaturase-1, enhance lipid oxidation and thermogenesis, and prevent free fatty acids from entering adipocytes for lipogenesis. PUFA also exert suppressive effects on several key factors involved in adipocyte differentiation and fat storage. Despite their similar effects and shared mechanisms, they display differences in the regulation of lipid metabolism. Moreover, DHA and EPA exhibit "anti-obesity" effect as well as improving insulin sensitivity, while CLA induces insulin resistance and fatty liver in most cases. A deeper and more detailed investigation into the complex network of anti-obesity regulatory pathways by different PUFA will improve our understanding of the mechanisms of body weight control and reduce the prevalence of obesity.     

杂多酸催化合成辛酸蔗糖酯

以蔗糖、辛酸为原料,杂多酸为催化剂合成辛酸蔗糖酯。用L16(45)正交设计优化实验,高效液相色谱法分析反应液组成。考察了催化剂种类和用量、反应温度、原料配比、反应时间等因素对辛酸蔗糖酯产率的影响,发现以二甲基亚砜为溶剂、蔗糖与辛酸摩尔比1∶9、磷钨酸用量为蔗糖质量的2.0%、110℃反应时间6h,蔗糖转化率达60%,产物产要是二酯。动力学研究发现,蔗糖反应级数为一级,反应表观速率常数为0.0059min-1(90℃)、0.0117min-1(110℃),反应表观活化能Ea=39.57kJ/mol。