Comparative analysis of volatile constituents between herbal pair flos lonicerae-caulis lonicerae and its single herbs

Gas chromatography-mass spectrometry(GC-MS) and the chemometric resolution method(alternative moving window factor analysis,AMWFA) were used for comparative analysis of volatile constituents in herbal pair(HP) flos lonicerae-caulis lonicerae(FL-CL) and its single herbs.The temperature-programmed retention index(PTRI) was also employed for the identification of compounds.In total,44,39,and 50 volatile chemical components in volatile oil of FL,CL and HP FL-CL were separately determined qualitatively and quantitatively,accounting for 87.22%,94.54% and 90.08% total contents of volatile oil of FL,CL and HP FL-CL,respectively.The results show that there are 32 common volatile constituents between HP FL-CL and single herb FL,33 common volatile constituents between HP FL-CL and single herb CL,and 10 new constituents in the volatile oil of HP FL-CL.     

Analysis of volatile components in herbal pair herba schizonepetae-ramulus cinnamomi

Analysis of volatile components in herbal pair （HP） herba schizonepetae-ramulus cinnamomi （HS-RC）, single herb HS and RC was carried out by gas chromatography-mass spectrometry （GC-MS） data and chemometric resolution method （CRM）. The two-dimensional data obtained from GC-MS instruments were resolved into a pure chromatogram and a mass spectrum of each chemical compound by CRM. In total, 47, 61 and 51 chemical components in volatile oil of HS, RC, and HP HS-RC were respectively determined qualitatively and quantitatively, accounting for 90.52%, 88.37%, and 88.72% total contents of volatile oil of HS, RC, and HP HS-RC, respectively. The number of the volatile components of HP HS-RC is almost the addition of that of two single herbs, but their relative contents are changed.     

Analysis of volatile components in herbal pair herba schizonepetae-ramulus cinnamomi

Analysis of volatile components in herbal pair (HP) herba schizonepetae-ramulus cinnamomi (HS-RC),single herb HS and RC was carried out by gas chromatography-mass spectrometry (GC-MS) data and chemometric resolution method (CRM).The two-dimensional data obtained from GC-MS instruments were resolved into a pure chromatogram and a mass spectrum of each chemical compound by CRM.In total,47,61 and 51 chemical components in volatile oil of HS,RC,and HP HS-RC were respectively determined qualitatively and quantitatively,accounting for 90.52%,88.37%,and 88.72% total contents of volatile oil of HS,RC,and HP HS-RC,respectively.The number of the volatile components of HP HS-RC is almost the addition of that of two single herbs,but their relative contents are changed.     

互叶白千层挥发性成分的提取和分析

采用水蒸气蒸馏法从互叶白千层叶及嫩枝中提取挥发油中的油相成分,用乙醚作为溶剂从蒸馏残液中萃取挥发油的水溶性物质,利用气相色谱-质谱（GC-MS）联用分析二者的化学成分并进行比较。互叶白千层挥发性成分的油相部分经鉴定确认了27种成分,占油相部分总量的98.60%,主要成分为松油烯-4-醇（52.15%）、γ-松油烯（19.60%）、α-松油烯（5.86%）、α-松油醇（3.93%）、对伞花烃（3.52%）、1,8-桉叶油素（2.67%）;水溶性部分经鉴定确认了61种成分,占水溶性部分总量的79.15%,主要成分为松油烯-4-醇（27.42%）、α-松油醇（6.55%）、香芹酚（5.00%）、1,8-桉叶油素（4.10%）。     

Determination of constituents of essential oil from Angelica sinensis by gas chromatography-mass spectrometry

Gas chromatography-mass spectrometry （GC-MS） coupled with chemometric resolution upon twodimensional data was employed to analyze the constituents of essential oils of Angelica sinensis. Constituents in essential oils of Angelica sinensis root were identified by GC-MS with the help of subwindow factor analysis （SFA） method resolving two-dimensional original data into mass spectra and chromatograms. 76 of 97 separated constituents in essential oil of Angelica sinensis root were identified and quantified, and they account for about 91.36% of the total content. The results show that ligustilide, butylene phthalide, 2-methoxy-4-vinylphenol, carvacrol, alloocimene, 2,6,6-trimethylbicyclo-[3,1,1] hept-2-ene are the main constituents in essential oil of Angelica sinensis root.     

加速溶剂萃取/气相色谱-质谱法分析朝鲜淫羊藿挥发油成分

为探究朝鲜淫羊藿挥发油的化学成分,采用加速溶剂萃取法（ASE）提取朝鲜淫羊藿挥发油,气相色谱-质谱联用（GC/MS）分析检测,在完全随机设计优化ASE的萃取条件下,萃取收率4.0%,通过NIST98谱图库检索,确认挥发油中包含91种化合物,并用峰面积归一化法,求得各化学成分在挥发油中的质量分数.结果表明：朝鲜淫羊藿挥发...     

Chemical component analysis of volatile oil in drug pair Herba Ephedrae-Ramulus Cinnamomi by GC-MS and CRM

Active volatile components in drug pair(DP)Herba Ephedrae-Ramulus Cinnamomi(HE-RC),single drug HE and RC were analyzed by gas chromatography/mass spectrometry(GC/MS),chemometric resolution method(CRM)and overall volume integration.By means of CRM,the two-dimensional data obtained from GC-MS instruments were resolved into a pure chromatogram and a mass spectrum of each chemical compound.In total,97,62,and 78 volatile chemical components in volatile oil of HE,RC,and DP HE-RC,were respectively determined qualitatively and quantitatively,accounting for 90.08%,91.62%,and 89.76% total contents of volatile oil of HE,RC,and DP HE-RC respectively.It is further demonstrated that the numbers of volatile components of DP HE-RC are almost the sum of those of two single drugs,but some relative contents of them are changed.Some new components,such as 1,6-dimethylhepta-1,3,5-triene,tetracyclo[4.2.1.1(2,5).0(9,10)]deca-3,7-diene,globulol and(E,E)-6,10,14-trimethyl-5,9,13-pentadecatrien-2-one are found in DP HE-RC because of chemical reactions and physical changes during decoction.     

Analysis of volatile chemical components of Radix Paeoniae Rubra by gas chromatography-mass spectrometry and chemometric resolution

The volatile chemical components of Radix Paeoniae Rubra (RPR) were analyzed by gas chromatography-mass spectrometry with the method of heuristic evolving latent projections and overall volume integration. The results show that 38 volatile chemical components of RPR are determined, accounting for 95.21% of total contents of volatile chemical components of RPR. The main volatile chemical components of RPR are (Z, Z)-9,12-octadecadienoic acid, n-hexadecanoic acid, 2-hydroxy- benzaldehyde, 1-(2-hydroxy-4-methoxyphenyl)-ethanone, 6,6-dimethyl-bicyclo[3.1.1] heptane-2-methanol, 4,7-dimethyl-benzofuran, 4-(1-methylethenyl)-1-cyclohexene-1-carboxaldehyde, and cyclohexadecane.     

Component analysis of volatile oil from Illicium Verum Hook. f.

Volatile oil was extracted from Illicium Verum Hook. f. by using steam distillation. 41 kinds of compounds were separated and identified by GC/MS, and their relative contents were determined by normalization method. Among 41 identified compounds, there are 14 hydrocarbon components and 22 oxygenated hydrocarbon derivatives, and a small amount of nitrogenous compounds. The main component is anethole, accounting for 76.23%, and the other components such as anisyl acetone, anisaldehyde, p-allylanisole, p-cumic aldehyde and p-allylpen take up more than 10%. Foundation item: Science Foundation of Education for Hunan Province (No. 00C324) Biography of the first author: YAN Jian-hui, associate professor, doctorate candidate, born in 1958, majoring in applied chemistry.     

Comparative analysis of chemical components between barks and leaves of Eucommia ulmoides Oliver

The chemical components of the essential oils in the barks and leaves of Eucommia ulmoides Oliver were analyzed and compared by chromatograms and mass spectra technique, heuristic evolving latent projections (HELP), alternative moving window factor analysis (AMWFA) algorithms and normalization method based on the peak areas; the flavones in the barks and leaves of Eucommia ulmoides Oliver were separated on an ODS column by gradient elution carried out with the flow phase consisting of water, methanol and phosphoric acid (0.1%), and their contents were quantitatively determined by standard curve method and diode array detection (DAD) at 362 nm. The results show that 68 and 73 compounds respectively from essential oils of the barks and leaves of Eucommia ulmoides Oliver are identified, and there are 33 mutual compounds among 108 compounds determined. The total contents of these volatile components of the two samples possess 92.9% and 97.75% of the gross of the relevant essential oils, respectively; the contents of the rutin, quercetin and kaempferol in the barks and leaves of Eucommia ulmoides Oliver are 0.016 9, 0.003 6, 0.002 1 and 0.064 4, 0.030 2, 0.010 0 mg/g, respectively, and the determination recoveries are 95.2%–106.2%. The comparative analysis shows that for the barks and leaves of Eucommia ulmoides Oliver, there are significant differences in their components of the relevant essential oils and flavones. Foundation item: Project(20235020) supported by the National Natural Science Foundation of China