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1.
Peter B. Kelly Raymond Reiser Donald W. Hood 《Journal of the American Oil Chemists' Society》1958,35(5):189-192
It appears that young mullet make excellent experimental fish for dietary studies. They are hardy, easily caught, resistant
to disease, and take well to aquaria conditions. Of particular interest, they may be fed synthetic food and they resist changes
in salinity well. These experiments indicate that, on a fatfree diet, the mullet does not synthesize the large amounts of
polyunsaturated fatty acids normally found in its body fat. However the mullet, like land animals, can apparently convert
dienoic acid from cottonseed oil into small amounts of tetraenoic, pentaenoic, and hexaenoic acids. Unlike land animals, the
mullet appears to be capable of the conversion of linoleic acid to a trienoic acid. Finally, when fed a diet containing a
typical marine fat such as menhaden oil, the mullet stores it almost unchanged. 相似文献
2.
Teruyoshi Yanagita Mikinobu Satoh Hisashi Nomura Noriyuki Enomoto Michihiro Sugano 《Lipids》1987,22(8):572-577
Effects of di-(2-ethylhexyl)adipate (DOA) and di-(2-ethylhexyl)phthalate (DEHP), plasticizers for polyvinylchloride products,
on concentrations and compositions of hepatic phospholipids were studied in rats. When administered to rats at a 2% level
for 2 wk, both DOA and DEHP caused a hepatomegaly, an increase in hepatic phospholipids and a decrease in the ratio of phosphatidylcholine
(PC) to phosphatidylethanolamine (PE). In the comparable study with mice, the alkyl moiety of DOA was found to be responsible
for these alterations. DOA and DEHP specifically altered fatty acid compositions of PC and PE: there was an increase in oleic
and palmitic acids and a decrease in stearic and docosahexaenoic acids in PC and an increase in arachidonic acid at the expense
of docosahexaenoic acid in PE. In addition, DOA caused an increase in the trienoic and tetraenoic molecular species in PC
and an increase in the 1-palmitoyl-2-arachidonyl (16∶0//20∶4) species in PE. Thus, the effects of DOA on the lipid dynamics
resembled those observed with DEHP, although the magnitude was slightly moderated. 相似文献
3.
The fatty acids of liver lipids from rats raised on a fat free diet from the 30th to the 90th day after birth were analyzed
with special regard to the detection of positional isomers of mono-, di-, tri-, and tetraenoic fatty acids. The methyl esters
obtained after transesterification of total lipids were separated by argentation chromatography into five fractions: I saturated,
II monoenoic, III dienoic, IV dienoic nonmethylene interrupted, V triand tetraenoic fatty acid esters. After hydroxylation
of the double bonds with osmium tetroxide, the analysis of the poly-O-trimethylsilyl derivatives by gas liquid chromatography
on S.C.O.T. columns combined with mass spectrometry revealed the presence of 19 monoenoic, 15 dienoic, and 9 trienoic as well
as 3 tetraenoic fatty acid isomers including the normally occurring representatives of the (n−3), (n−6), (n−7), and (n−9)
fatty acid families. The majority of the identified isomers can be coordinated to one of these families like 7–16∶1; 11–20∶1;
6,9–18∶2; 8,11–20∶2; 5,11–20∶2; 5,8,11–20∶3; 7,10,13–22∶3 to the (n−9) family, 11–18∶1; 13–20∶1; 5,11–18∶2; 7,13–20∶2; 6,11–18∶2;
6,9–16∶2; 8, 11–18∶2; 10,13–20∶2; 5,8,11–18∶3; 7,10,13–20∶3; 4,7,10,13–20∶4 to the (n−7) family and 11,14–20∶2; 5,11,14–20∶3;
6,9,12–18∶3; 8,11,14–20∶3; 5,8,11,14–20∶4; 7,10,13,16–22∶4 to the (n−6) family. All these naturally occuring isomers can be
placed into a network of desaturation and chain elongation steps which allows certain conclusions about the substrate specificity
of the Δ6-, Δ5-and Δ4-desaturase systems. The great number of isomers found in the (n−7) family indicates that the members
of this family are actively metabolized in partial essential fatty acid deficiency. 相似文献
4.
The molecular species composition of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylserine (PS) from
white muscle, liver, retina and brain of cod (Gadus morhua) were determined by high-performance liquid chromatography of the respective 1,2-diacylglycerol 3,5-dinitrobenzoyl derivatives.
A minimum of 69 diacyl species was identified. In muscle and liver saturated fatty acid/polyunsaturated fatty acid (PUFA)
and monounsaturated fatty acid/PUFA molecular species were predominant, particularly 16∶0/20∶5 and 16∶0/22∶6 in PC, 16∶0/22∶6
and 18∶1/22∶6 in PE and 18∶0/22∶6 and 18∶1/22∶6 in PS. Didocosahexaenoyl species were major components of PC, PE and PS from
retina, comprising 29.3, 71.8 and 59.7% of the respective totals. Didocosahexaenoyl species were also abundant in PE and PS
from brain, accounting for 13.8 and 24.0% of the totals, respectively. DiPUFA species were important in muscle, totalling
21.2% in PC and 38.3% in PE. PC from all tissues had the largest amounts of species containing only saturated or monounsaturated
fatty acids, accounting for 59.8% of PC from brain, including 12.8% of 18∶1/24∶1 plus 24∶1/18∶1. 相似文献
5.
The uptake and distribution of three common dietary polyunsaturated fatty acids was studied using Chinese hamster lung fibroblasts
(V79 cells). Treatment of V79 cells with arachidonic (20∶4), eicosapentaenoic (20∶5) and dihomogammalinolenic (20.3) acids
for 24 hr produced a marked uptake of 20∶3 and 20∶4, both of which were assimilated to a considerably greater degree than
20∶5. All polyunsaturated fatty acids were incorporated primarily into phospholipids; however, there were considerable differences
in their distribution into individual phospholipid species. Although 20∶4 was incorporated primarily into phosphatidylcholine,
20∶3 entered largely into phosphatidylethanolamine and phosphatidylglycerol, and 20∶5 was distributed about equally between
phosphatidylcholine, phosphatidylethanolamine and phosphatidylinositol. A marked conversion of 20∶3 to 20∶4 was found after
24 hr and, in several phospholipids, there was as much derived-radiolabeled 20∶4 as there was radiolabeled 20∶3. There was
little evidence of 20∶4 and 20∶5 metabolism. V79 cells undergo substantial changes in phospholipid fatty acid composition
following supplementation with these polyunsaturated fatty acids; however, these fatty acids are assimilated to different
degrees and their distribution among cellular phospholipids is distinct, suggesting incorporation via independent mechanisms. 相似文献
6.
Molecular architecture and biophysical properties of phospholipids during thermal adaptation in fish: An experimental and model study 总被引:1,自引:0,他引:1
Elfrieda Fodor Richard H. Jones Csaba Buda Klara Kitajka Indranil Dey Tibor Farkas 《Lipids》1995,30(12):1119-1126
Phospholipids from livers of carps (Cyprinus carpio L.) adapted to winter (5°C) and summer (25°C) temperatures were isolated, and the fatty acid composition of total phospholipids,
as well as molecular species composition of diacyl phosphatidylcholines and ethanolamines, were determined. Order parameter
of 5-doxyl stearic acid and steady-state fluorescence anisotropy of different anthroyloxy fatty acids—[2-, 12(N-9-anthroyloxy)stearic acid and 16(N-9-anthroyloxy)palmitic acid—embedded in native and synthetic (16∶0/16∶0, 16∶0/22∶6, 18∶0/22∶6, 18∶1/22∶6, 20∶4/20∶4, 22∶6/22∶6
phosphatidylcholines and 16∶0/18∶1, 18∶1/22∶6 phosphatidylethanolamines) phospholipid vesicles was also determined between
−30 and 30°C and 5 and 30°C, respectively. There is an accumulation of 1-monoenoic, 2-polyenoic diacyl phosphatidylcholine
and ethanolamine with a concomitant reduction of 1-stearoyl,2-docosahexaenoyl species in the cold-adapted state. Despite a
30% accumulation of long-chain polyunsaturated fatty acids in phospholipids in cold, there is only a 5°C downshift in the
solid-gel to liquid-crystalline phase transition temperature (−8 vs. −13°C). Vesicles from total phospholipids of cold-adapted
fish proved to be more disordered in all segments than from the warmadapted ones when assayed using 2,12-(N-9-anthroyloxy)stearic and 16-(N-9-anthroyloxy)palmitic acid. Vesicles made from purified phosphatidylcholines showed the same pattern, but they were more
disordered than the corresponding total phospholipids. This could be modelled using mixed phospholipid vesicles made of synthetic
16∶0/22∶6 phosphatidylcholine (75%) and either 18∶1/22∶6 phosphatidylethanolamine (25%) vs. 16∶0/18∶1 phosphatidylethanolamine
(25%) and comparison of the anisotropy parameters of 100% 16∶0/22∶6 and 100% 18∶1/22∶6 phosphatidylcholine vesicles. Mixing
either 16∶0/18∶1 (25%) or 18∶1/22∶6 (25%) phosphatidylethanolamines to 18∶0/22∶6 (75%) phosphatidylcholine shifted down or
up, respectively, the transition temperature of vesicles compared to 100% 18∶0/22∶6 vesicles assayed by electron spin resonance
spectroscopy using 5-doxylstearic acid. It is concluded that it is not the gross amount of long-chain polyunsaturated fatty
acids in phospholipids, but rather their specific combination withcis Δ9 monounsaturated fatty acids in the positionsn-1, especially in phosphatidylethanolamines, that is important in determining the physical properties of biomembranes in relation
to adaptational temperature. 相似文献
7.
The present study examined the effects of two atherogenic factors, animal protein and cholesterol, on the distribution of
fatty acids and the molecular species of major liver phospholipids in mice. Weanling mice were fed a semisynthetic diet supplemented
with either casein or soy protein (20%, w/w) in the presence or absence of 0.5% cholesterol for 4 wk. Results from mouse liver
showed that animal protein and, more so, dietary cholesterol modified the fatty acid profiles of the phospholipids. Animal
protein had no significant effect on the concentration of lipids, but it altered the relative distribution and fatty acid
profiles of the phospholipids, phosphatidylcholine and phosphatidylethanolamine. Dietary cholesterol, on the other hand, significantly
increased the concentration of liver lipids, but it did not alter the relative distribution of phosphatidylcholine and phosphatidylethanolamine.
In cholesterol-fed mice, the proportions of molecular species containing 18∶2n−6 were increased, whereas those containing
20∶4n−6 were decreased, indicating that dietary cholesterol suppressed linoleic acid metabolism. Since cholesterol feeding
selectively decreased the ratio of 18∶0/20∶4n−6 in phosphatidylcholine, whereas it increased the 18∶0/18∶2n−6 ratio in phosphatidylethanolamine,
this finding suggests that dietary cholesterol may affect the incorporation of fatty acids but not the rate of synthesis of
phosphatidylcholine and phosphatidylethanolamine. 相似文献
8.
Domart Claude Miyauchi D. T. Sumerwell W. N. 《Journal of the American Oil Chemists' Society》1955,32(9):481-483
Summary In two series of experiments, marine-animal-oil fatty acids were fractionated with urea using methanol as solvent.
In the first series, menhaden-oil fatty acids were fractionated at 1°C. Almost all the saturated and monoenoic fatty acids
were removed at mole ratios of 12∶1 to 13∶1. At higher ratios increasing amounts of the less stable dienoic fatty acids were
precipitated. By the use of the appropriate ratio, fractions having iodine values above 300 were prepared.
In the second series, fatty acids from the oils of menhaden, herring, tuna, seal, salmon eggs, and salmon heads and viscera
were fractionated at a mole ratio of urea to fatty acid of 9.2∶1. At 25° and 1° the complexes were composed almost entirely
of saturated and monoenoic fatty acids, but as the temperature was lowered to −30°, the content of dienoic fatty acids in
the precipitates increased.
Presented at the Regional American Chemical Society Meeting, Richland, Wash., June 11–12, 1954.
One of the laboratories of the Branch of Commercial Fisheries, Fish and Wildlife Service, U. S. Department of the Interior. 相似文献
9.
The fatty acid composition of individual phospholipids in subcellular fractions of sheep platelets and the asymmetrical distribution
of phosphatidylethanolamine (PE) fatty acyl chains across the plasma membrane were examined. The main fatty acids of total
lipid extracts were oleic (18∶1; 32–41%), linoleic (18∶2, 10–17%), stearic (18∶0; 13–15%), palmitic (16∶0; 11–15%) and arachidonic
(20∶4; 8–12%) acids, with a saturated/unsaturated ratio of about 0.4. Each phospholipid class had a distinct fatty acid pattern.
Sphingomyelin (SM) showed the highest degree of saturation (50%), with large proportions of behenic (22∶0), 18∶0 and 16∶0
acids. The main fatty acid in PE, phosphatidylserine (PS) and phosphatidylcholine (PC) was 18∶1n−9. Our findings suggest that
fatty acids are asymmetrically distributed between thecholineversus the non-choline phospholipids, and also between plasma membranes and intracellular membranes. The transbilayer distribution
of PE fatty acids in plasma membranes from non-stimulated sheep platelets was investigated using trinitrobenzenesulfonic acid
(TNBS). A significant degree of asymmetry was found, which is a new observation in a non-polar cell. The PE molecules from
the inner monolayer contained higher amounts of 18∶2 and significantly less 18∶1 and 20∶5 than those found in the outer monolayer,
although no major differences were detected in the transbilayer distribution of total unsaturatedversus saturated PE acyl chains. 相似文献
10.
Cod (mean start weight of 26 g) were fed three diets for 15 months, each based on a dry pellet coated at a level of 9g/100
g with soybean oil, capelin oil or sardine oil. The fatty acid compositions of neutral lipids and four glycerophospholipids
of white muscle, liver, gills and heart were determined. The fatty acid composition of dietary lipids influenced the composition
of neutral lipids in all organs. Linoleic acid (18∶2n−6) from soybean oil was selectively incorporated into phosphatidylcholine
of the four tissues. Similar levels of 20∶5n−3 and 22∶6n−3 in phosphatidylcholine and phosphatidylethanolamine were found
in all organs from cod fed capelin oil and sardine oil in spite of highly differentiated feed fatty acid levels. The polyunsaturated
fatty acid (PUFA) composition of phosphatidylinositol was least influenced by dietary lipids. The preferred monoenic fatty
acid in phospholipids of cod was 18∶1n−9, independent of dietary intake, whereas the longer chain monoenoic acids seemed to
be preferentially catabolized. The results suggest that 20∶4n−6 as well as 20∶5n−3 and 22∶6n−3 fatty acids are essential for
cod. 相似文献
11.
Chromatographic resolution of phospholipids from the flesh of the South African pilchard(Sardina ocellata Jenyns) revealed the presence of cardiolipins, cephalins, inositol phosphatides, cerebrosides, sphingomyelins, lecithins, lysoleci-thins
and plasmalogens. Column and paper Chromatographic techniques were used to identify ethanolamine, serine, cho-line, sphinogsine
and inositol in pilchard phos-pholipid hydrolysates. The fatty acids of pilchard phospholipids com-prised large amt of C22 hexaenoic, C20 pentaenoic and C16 saturated acids, with smaller amt of C18 dienoic, C18 monoenoic and C18 saturated acids. 相似文献
12.
Minimal deviation hepatoma cells were cultured in a modified Swim's 77 medium supplemented with decreasing amounts of serum,
lipid-free serum, and lipid-free serum containing added palmitic or linoleic acids. Cellular phospholipids were extracted
and the class distribution determined quantitatively. The fatty acid composition of each phospholipid class was determined,
and the percentages from cells grown on each of the various media were compared. Cellular phospholipid class and fatty acid
compositions differed from media compositions, indicating that intact serum phospholipids are not incorporated into cellular
structures. Phosphatidylcholine percentages decreased as the media serum and lipid levels decreased, while phosphatidylinositol
and phosphatidylethanolamine percentages increased. Sphingomyelin of cells grown in medium containing added linoleic acids
contained a high level of a 24∶2 acid. All classes, except sphingomyelin, contained elevated levels of 18∶1 acid and decreased
levels of polyunsaturated fatty acids, relative to normal rat liver. Cells cultured on lipid-free medium did not contain increased
concentrations of 20∶3 acid, suggesting that this hepatoma cell cannot desaturate monoenoic acids. Phosphoglycerides of cells,
grown on lipid-free medium, had the highest monoene fatty acid concentration, whereas those cells grown on media containing
added linoleic acid had the lowest concentrations, suggesting that linoleate may inhibit or regulate monoenoic acid biosynthesis
in this cell. These mass data also demonstrate that monoenoic fatty acid biosynthesis in this cultured hepatoma cell responds
to dietary changes. 相似文献
13.
Synthesis of phosphatidylcholine with (n−3) fatty acids by phospholipase A 2 in microemulsion 总被引:3,自引:0,他引:3
An Na Caj Eriksson Sven-Göran Eriksson Eva Österberg Krister Holmberg 《Journal of the American Oil Chemists' Society》1990,67(11):766-770
Phosphatidylcholine containing a long chain polyunsaturated acyl group at the 2-position has been prepared by phospholipase
A 2 catalyzed esterification of lysophosphatidylcholine with polyunsaturated fatty acids EPA (C20∶5) or DHA (C22∶6). Preliminary
studies showed that the other fatty acids, such as lauric acid (C12), palmitic acid (C16), stearic acid (C18) and linoleic
acid (C18∶2), were also incorporated. To our knowledge, phospholipase A 2 catalyzed condensation reactions have not been reported
in the literature before. The reactions were performed in sodiumbis(2-ethylhexyl)-sulfosuccinate-based microemulsions containing small amounts of water. Synthesis of the same phospholipid by
transesterification of phosphatidylcholine with the polyunsaturated acids in microemulsion failed; however, enzymatic hydrolysis
to lysophosphatidylcholine was facile, quantitative conversion from phosphatidylcholine being attained after 16 hr reaction
time.
An additional observation was that, unlike enzymatic hydrolysis of phospholipids, the condensation reaction catalyzed by phospholipase
A 2 was totally independent of the presence of calcium. 相似文献
14.
M. F. Pediconi L. E. Politi C. B. Bouzat E. B. De Los Santos F. J. Barrantes 《Lipids》1992,27(9):669-675
Phospholipid and neutral lipid composition was studied in the course of myogenic differentiation of the clonal cell line BC3H-1.
Total phospholipid content increased during differentiation, predominantly in the major classes of choline and ethanolamine
glycerophospholipids. The contents of other lipids, such as triacylglycerols, diminished more than 50% during this period.
The content and distribution of fatty acids also underwent marked differentiation-dependent changes. The polyunsaturated (tetrapenta-
and hexaenoic) fatty acid species of several phospholipid classes diminished during differentiation, especially those in choline,
serine and inositol glycerophospholipids. Most noticeable were the changes in phosphatidylserine; long-chain fatty acids having
20 to 22 carbon atoms and 4 to 6 double bonds decreased from about 30 to about 10 mol%. Although increased levels of saturation
in other phospholipid fatty acyl chains appear to accompany the myogenic changes of BC3H-1 cells, some unsaturated fatty acids,
such as oleic acid (18∶1), increased by as much as 80% during the same period, suggesting the activation of a Δ9 desaturase.
Sphingomyelin contained only saturated and monoenoic fatty acids and exhibited a four- to five-fold decrease in its content
of monoenoic acyl groups. Diacylglycerols became enriched in arachidonate and docosahexaenoate. The amount of cholesterol
and its esters increased slightly during differentiation of BC3H-1 cells. The data show that several metabolic pathways change
during myogenic differentiation of the BC3H-1 clonal cell line, particularlyde novo biosynthetic pathways, elongation/desaturation reactions, and acyl chain turnover. As a consequence of this, the lipid composition
of the myoblast form of the BC3H-1 cell, in which the nicotinic acetylcholine receptor and other cell surface receptors are
expressed, is thus different from that of the nondifferentiated cell. 相似文献
15.
The incorporation of3H-inositol into the molecular species of rat liver monophosphoinositides was studied as a function of time. At early time
intervals following intraperitoneal injection of the tracer, the specific activity of the monoenoic and dienoic species exceeded
that of the total and tetraenoic inoisitides by 7- and 14-fold. From 90 min to 9 hr, the specific activity of the unfractionated
phosphatide remained nearly constant while the pronounced decrease in activity [of the monoenoic and dienoic subfractions
was concomitant with an increase in the radioactivity of the tetraenoic species, as found previously with32P- and14C-glycerol. The present results suggest that the entire glycerophosphorylinositol backbone originally associated with the
monoenoic and dienoic monophosphoinositides is converted into tetraenoic species with remarkable conservation of label in
vivo. 相似文献
16.
Membrane lipid metabolism and phospholipase activity in insect Spodoptera frugiperda 9 ovarian cells
Although there is increasing use of insect ovarian Sf9 cells for the production of recombinant proteins, namely, via the baculovirus
vector expression system, little is known about the lipids in the cell membrane and whether endogenous phospholipases are
present for regulation of the cell membrane lipids. In this study, analysis of membrane lipids of Sf9 cells indicated the
presence of phosphatidylethanolamine (PE) (diacyltype) and phosphatidylcholine as major phospholipids, followed by phosphatidylserine
and phosphatidylinositol (PI), and only trace amounts of ethanolamine plasmalogen. These phospholipids contain high proportions
of monoenoic fatty acids, e.g., 16∶1 and 18∶1, which comprise more than 70% of the total fatty acids although small amounts
of polyunsaturated fatty acids such as 18∶2 and 20∶4 are also present. When Sf9 cells were incubated in a culture medium containing
[14C]oleic acid and [14C]arachidonic acid, a large portion of the labels were incorporated into membrane phospholipids. Using [14C]arachidonoyl-phospholipids as substrates for incubation with cell homogenate and subcellular fractions, results indicate
the presence of a Ca2+-independent phospholipase A (PLA2) in the Sf9 cell cytosol fraction. This PLA2 shows a high preference for hydrolysis of PE and is active at a pH range of 7–9. Unlike the brain cells which contain active
phospholipase C (PLC) specific for phosphatidylinositol, only limited amount of diacylglycerol (DAG) was released from [14C]arachidonoyl-PE in the Sf9 cells. Taken together, this study demonstrates active metabolism of membrane phospholipids in
Sf9 cells, most likely mediated by acyltransferases and PLA2. Furthermore, despite the absence of PLC for PI, limited amount of DAG could be generated through hydrolysis of PE. 相似文献
17.
We have designed a rapid method for the separation of polyunsaturated fatty acids (PUFA, ≥trienes) from non-PUFA, and for
estimation of total amounts of PUFA in lipids of aquatic organisms. Lipids from thirty-one species, including marine and fresh
water fishes, shell fishes, marine algae, and other aquatic animals, and from terrestrial organisms, were transesterified
with sodium methoxide in methanol. The resulting fatty acid methyl esters were separated by thin-layer chromatography on commercially
available plain silica gel plates with a developing solvent ofn-hexane/ethyl ether/acetic acid (95∶5∶1, by vol). All of the methyl esters from aquatic organisms tested separated into two
spots, whereas those from terrestrial sources, except for linseed oil, showed a single unresolved spot. The upper and lower
spots were scraped separately from the plate, and their fatty acid compositions were determined by gas-liquid chromatography.
The lower spot was composed of PUFA having more than two double bonds, whereas components of the upper spot were saturated,
monoenoic, and the greater part of the dienoic fatty acids. When the spots on the silica gel plate were stained with Coomassie
brilliant blue, the amounts of PUFA in aquatic organisms could be estimated satisfactorily using a scanning densitometer.
Presented in Japanese at the general Meeting of JSSF held in Mie University, Tsu-city, Japan, October 1994. 相似文献
18.
Lipids were extracted from the roots and shoots of four species of theCapsicum (pepper) genus and separated into three fractions: triglycerides; free fatty acids, mono- and diglycerides; and phospholipids.
The component fatty acids were determined by subjecting the methyl esters to gasliquid chromatography. The predominate fatty
acids obtained were palmitic (16∶0) and linoleic (18∶2), with lesser amounts of linolenic (18∶3), stearic (18∶1), and oleic
(18∶0). Differences existed in the neutral lipid fractions which might be of value from taxonomic interests; however, the
phospholipids from each of the species and plant parts did not differ so greatly. A comparison of the amount of unsaturated
fatty acids in the phospholipid fractions indicates that differences exist which might be of value in determining the relative
sensitivity of the several species to chilling temperatures. 相似文献
19.
Georges Zwingelstein Gerard Brichon Roger Meister Chantal Maury Ion Gresser 《Lipids》1987,22(10):736-743
Suckling mice were injected daily from birth for 10 days with potent preparations of mouse interferon α/β. Interferon treatment
resulted in a markedly lower concentration of polyunsaturated fatty acids (20∶4ω6 and 22∶6ω3) in the two principal liver phospholipids,
phosphatidylcholine and phosphatidylethanolamine, than in livers of control-treated mice. This effect appeared to correlate
with a low level of synthesis of polyunsaturated phospholipids in the livers of interferon-treated mice. Thus, in control
mice, synthesis of species of polyunsaturated phospholipids increased markedly in the first 10 days of life, whereas in 10-day-old
interferontreated mice, the level of synthesis of species of polyunsaturated phospholipids was comparable to that in newborn
mice. In parallel, a marked increase in the diacylglycerol content without change of its renewal was observed in the livers
of interferon-treated mice. We suggest that interferon treatment results in an inhibition of one of the processes that leads
to activation of the enzymatic systems responsible for the synthesis of species of polyunsaturated phosphatidylcholine and
phosphatidylethanolamine in the liver of suckling mice. It seems likely that these results are related to the inhibition of
liver cell maturation and the marked cell necrosis that are observed in interferon-treated suckling mice. 相似文献
20.
A simple, high-performance liquid chromatographic method is described for the separation of tetraenoic, trienoic and dienoic
conjugated fatty acids on a Zorbax ODS reversed-phase column using acetonitrile/tetrahydrofuran (95∶5, vol/vol) at a flow
rate of 1.2 mL/min as mobile phase. Also described is the separation of the isomeric conjugated fatty acids with acetonitrile/water/tetrahydrofuran
(90∶90∶1, by vol) as mobile phase. The simultaneous detection and identification of the separated geometrical isomers in the
eluant was accomplished using photodiode array detection. 相似文献