Effect of source of dietary fat on pig performance,carcass characteristics and carcass fat content,distribution and fatty acid composition

Seventy gilts were used to compare the effect of including 10% tallow (T), high-oleic sunflower oil (HOSF), sunflower oil (SFO), linseed oil (LO), a fat blend (FB), or an oil blend (OB) in finishing diets vs. feeding a semi-synthetic diet with no added fat (NF) on pig performance, carcass traits and carcass fatty acid (FA) composition. Carcasses from SFO-fed gilts had greater fat and lower lean compositions than carcasses from T-fed gilts. Gilts fed NF had greater loin fat than FB-fed gilts, and greater flare fat, loin intermuscular fat and fat:lean than T-fed gilts. Bellies from NF-fed gilts had lower lean and higher intermuscular fat and fat:lean than other diets except HOSF. Fat source had minor effects on animal performance, carcass characteristics and carcass fat content and distribution, whereas feeding NF resulted in carcasses and major cuts with higher fat content. Diets rich in polyunsaturated FA (PUFA) did not reduce fat deposition in separable fat depots with respect to monounsaturated FA (MUFA) and saturated FA (SFA). Carcasses from gilts fed NF had a high degree of saturation (40.6% SFA) followed by carcasses of T- and FB-fed gilts. Feeding HOSF, SFO and LO enriched diets elevated the percentages of MUFA (56.7%), n−6 (30.0%) and n−3 (16.6%) PUFA, respectively, whereas carcasses from gilts fed OB had greater percentages of n−3 FA (14.8% n−3, 0.9% EPA, 1.0% DPA, 3.1% DHA) than gilts fed FB (6.72% n−3, 0.1% EPA, 0.4% DPA, 0.1% DHA).     

肌内脂肪与背最长肌肉质、脂肪酸组成的关系

研究肌内脂肪（IMF）含量与肉质以及脂肪酸含量和组成比例之间的关系。以北京黑猪背最长肌（n=20）为实验材料,测定肌内脂肪含量、肉质以及脂肪酸含量和组成。结果显示,肌内脂肪含量与滴水损失呈显著正相关（p<0.05）,与蒸煮损失、剪切力、肉色的相关性不显著（p>0.05）。随着肌内脂肪含量升高,除长链多不饱和脂肪酸(C_20:3n6、C_20:4n6)以外的大多数脂肪酸含量增加,相对比例也上升;多不饱和脂肪酸的含量增加,但是相对比例下降;长链多不饱和脂肪酸的含量和相对比例都降低。随着肌内脂肪含量的升高,饱和脂肪酸（SFA）和单不饱和脂肪酸（MUFA）的含量升高,回归方程斜率分别为197.3和248.31,多不饱和脂肪酸（PUFA）增加的幅度不大,回归方程的斜率为11.43。结果提示:肌内脂肪含量较低时（本实验平均值2.86%）,对肉质的影响不显著。随着肌内脂肪含量升高,总脂肪酸含量（TFA）增加,主要归因于SFA和MUFA含量的增加。PUFA的含量相对稳定,受肌内脂肪含量变化的影响较小。      

Changes in texture, colour and fatty acid composition of male and female pig shoulder fat due to different dietary fat sources

Two experiments with 72 slaughter pigs in each were conducted. Entire males and females were individually fed restricted. Palm kernel-, soybean- and fish-oil were used in varying combinations, giving different dietary fat levels (29-80 g/kg) and iodine values ranging from 50 to 131. Shoulder fat was analysed for fatty acid composition (inner and outer layer), firmness and colour. A clear dose-response relationship was seen between fatty acids in diets and in shoulder fat. Interestingly, the very long chain n-3 fatty acids seemed to be deposited more efficiently when additional fat was included in the diet. Both high and low dietary iodine values changed towards less extreme iodine values in fat. Low-fat diets enhanced de novo synthesis of fatty acids. Males revealed a higher percentage of PUFA and a lower percentage of C18:1 and MUFA. Fat firmness, but not colour, was influenced by sex and dietary fat source.     

Dietary CLA alters intramuscular fat and fatty acid composition of pig skeletal muscle and subcutaneous adipose tissue

The present study was conducted to determinate the responsiveness of different levels of dietary conjugated linoleic acid (CLA) on intramuscular fat (IMF) and fatty acid composition of skeletal muscle and fat in pigs fed from 59.5 to 133.5 kg. Forty female Large White × (Large White × Landrace) pigs were used. Four levels (0%, 0.5%, 1% and 2%) of a commercial enriched CLA oil supplementation (60% of CLA isomers, 30% cis-9, trans-11 and 30% trans-10, cis-12) were fed to pigs. Carcass, ham, foreleg and loin weights were recorded. Dietary CLA enrichment increased the loin weight (P < 0.01) and the combined weights of hams + forelegs + loins (P < 0.02). IMF content in Longissimus dorsi was also increased by dietary CLA treatment (P < 0.001) and a linear response was observed. Dietary CLA increased saturated fatty acids (SFA) and decreased monounsaturated fatty acids (MUFA) in muscle and adipose tissue (P < 0.001). Feeding 1% CLA to finishing swine increases IMF in heavy pigs slaughtered at an average weight of 133.5 kg.     

Protein,fat and fatty acid composition of Celosia species

The seeds of 13 lines of Celosia referable to four species were analysed for protein, fat and fatty acid composition. The protein contents varied from 101 to 170 g kg^?1 and fat contents from 56 to 109 g kg^?1. The fatty acid composition was of the simple palmitic-oleic-linoleic type.     

坚果中脂肪含量及脂肪酸组成分析

目的分析16种市售坚果中脂肪含量及脂肪酸组成,比较不同品种坚果间脂肪含量、饱和脂肪酸和不饱和脂肪酸组成的差异。方法采用气相色谱分析法测定坚果中37种脂肪酸的含量,用最小显著性差异法(least-significant difference,LSD)法进行多重比较。结果坚果的平均脂肪含量为(55.6±0.86)g/100 g,变化范围为40.2~71.3 g/100 g,碧根果脂肪含量显著高于其他品种坚果脂肪含量(P0.05);坚果中的不饱和脂肪酸含量为72.77~95.47 g/100 g脂肪,平均值为(86.68±0.12)g/100 g脂肪,显著高于饱和脂肪酸4.53~22.19 g/100 g脂肪,平均值为(11.31±0.16)g/100 g脂肪(P0.05),东北榛子中单不饱和脂肪酸总含量最高,为84.34 g/100 g脂肪,而纸皮核桃中的多不饱和脂肪酸总含量最高,为74.17 g/100 g;坚果中不含短链脂肪酸和中链脂肪酸。结论坚果中不饱和脂肪酸含量较多,富含ω-6和ω-3脂肪酸,营养价值较高。     

Effect of a high dose of CLA in finishing pig diets on fat deposition and fatty acid composition in intramuscular fat and other fat depots

Sixteen gilts were fed a control (4% of sunflower oil) or an experimental diet (4% conjugated linoleic acid (CLA) oil). CLA had no effect on intramuscular fat (IMF) content neither in longissimus thoracis (LT) nor in semimembranosus (SM) muscles but increased liver weight, reduced perirenal fat and tended to reduce backfat between the last 3th–4th lumbar vertebrae. Despite the fact that 9c,11t and 10t,12c CLA isomers were included in the same proportion in the diet, the 9c,11t and 9c,11c were the isomers more deposited in all tissues. Addition of CLA in the diet affected fatty acid composition in a tissue specific manner, increasing percentages of SFA in all tissues, reducing percentages of MUFA in LT and LT subcutaneous fat, and of PUFA in LT subcutaneous fat, liver and SM. The FA modification by dietary CLA in LT IMF was reflected in the different lipid fractions, SFA and MUFA mainly in the neutral lipid fraction, and PUFA in the polar fraction.     

A relationship between bovine fat colour and fatty acid composition

Subcutaneous adipose tissue was obtained from pasture-grazed (n = 13) and short-term (70 days) grain-fed (n = 13) cattle. The yellow colour of the adipose tissue was assessed by Minolta b(?) value readings and by determination of total carotenoids and the two measurements gave a correlation coefficient of 0·79 (P < 0·01). The fatty acid composition of the samples varied with fat colour. As the b(?) value and the carotenoid content of the fat increased, there was a significant increase in the total percentage of cis mono-unsaturated fatty acids and a decrease in saturated fatty acids (P < 0·01). Consequently, the ratio of cis mono-unsaturated to saturated fatty acids was found to be higher in those samples exhibiting a greater yellow colour.     

Genotype with nutrition interaction on fatty acid composition of intramuscular fat and the relationship with flavour of pig meat

Forty-one autumn-born Friesian bull calves were allocated to two production systems (Extensive='E'and Intensive='I'). In the E-system, animals were loose-housed and fed a roughage-based diet from October to May, followed by a grazing period from May to October. Ten animals were slaughtered directly from pasture in October [360 kg body weight (BW)] and 11 after a 10-week finishing period in tie-stalls (460 kg). The E-bulls were compared with intensively-fed tie-stall-housed young bulls (I) slaughtered at comparable weights (360 kg, n=11 and 460 kg, n=9). The myofibril fragmentation index (MFI) was measured 24 h post mortem in semitendinosus (ST), longissimus dorsi (LD), and supraspinatus (SU) muscles, and meat quality characteristics and sensory evaluation of LD were performed on aged meat. Intramuscular fat content was lower (P<0.001) in all three muscles of E- compared with I-bulls. MFI of ST and LD was lower in E-bulls compared with I-bulls, but only at 360 kg. In contrast, MFI of SU was higher in E- compared with I-bulls at 360 kg. In E- compared with I-bulls, shear force value of ST was higher (P<0.003) at 360 kg, but not at 460 kg. Panel scores for tenderness, taste and juiciness were all lower (P<0.006 to 0.001) and remarks for off-flavour higher in E- compared with I-bulls, the effects being most pronounced at 360 kg. A 10-week finishing period improved all meat and eating quality characteristics of E-bulls. In LD, the correlation between MFI and tenderness was 0.79 (P<0.001), which indicates a potential of MFI as an early predictor of tenderness.     

不同部位猪脂的总脂肪酸组成及Sn-2位脂肪酸成分分析

选择11例猪的4个部位(猪尾巴、猪脖、奶腩和猪板),用乙酸甲酯萃取脂肪,采用DIKMA CP-SIL88FS(60 m×0.25 mm id×0.20μm)毛细管柱气相色谱对各部位猪脂总脂肪酸及Sn-2位脂肪酸组成进行了测定,并采用SPSS 13.0软件对数据进行统计分析。结果表明:猪尾巴油、猪脖油和奶腩油的总脂肪酸及Sn-2位脂肪酸组成和分布与猪板油相比均存在显著性差异,且不饱和脂肪酸和单不饱和脂肪酸含量均高于猪板油。     

Oxidation of muscle phospholipids in relation to their fatty acid composition with emphasis on volatile compounds†

The impact of moderate changes of fatty acid (FA) composition of phospholipids (PL) on their oxidative stability and on volatile profiles remains largely unknown. PL of breast muscle of turkeys fed a diet containing 6% tallow, rapeseed oil or soya oil were purified and prepared as liposomes. After 24 h of incubation at 25 °C with iron/ascorbate, oxidation was quantified by measurement of thiobarbituric acid reactive substances (TBA‐RS) and volatile compounds. TBA‐RS level was the lowest (21.4 nmole eq MDA mg⁻¹ PL) in PL from tallow‐fed animals but was not significantly different (P > 0.05) in soya oil (30.9 nmole eq MDA mg⁻¹ PL) and rapeseed oil (30.3 nmole eq MDA mg⁻¹ PL) batches. ANOVA did not clearly distinguish between the three groups according to the quantities of individual volatiles except for Z,E‐2,4‐heptadienal. In contrast, principal component analysis (PCA) performed on standardised quantities of volatile compounds distinguished unambiguously the three groups. Axis 1 was positively correlated with volatile compounds arising from oxidation of n‐6 fatty acids, and negatively with compounds of n‐9 origin. Axis 2 was highly positively correlated with compounds from n‐3 origin. Only a few compounds from each origin (n‐6, n‐3, n‐9) had an atypical behaviour. A weak modification of the FA composition of PL led to concomitant modifications of the quantities of volatile compounds generated through oxidation, which were emphasised by multivariate analysis (PCA). © 1999 Society of Chemical Industry     

Seasonal variation in fatty acid and triacylglycerol composition of bovine milk fat

The aim of this study was to assess the effects of seasonal variation on the changes of the fatty acid (FA) and triacylglycerol (TAG) composition of bovine milk fat (MF) in a nonseasonal milking system. Weekly milk samples were collected from 14 dairy factories and pooled per week as representative samples of the average Dutch bovine milk. The sample collection started in May 2017 and finished in April 2018, resulting in a total of 52 samples, corresponding to each week of the year. The samples were analyzed for MF content (%) and FA and TAG composition using gas chromatography with flame-ionization detection. The increased intake of C18:3 cis-9,12,15 through grass feeding in spring and summer was associated with major changes in MF FA composition, including reduced proportions of de novo synthesized FA and presence of several rumen biohydrogenation products and conjugated linoleic acid isomers in MF. These changes in seasonal FA composition had an effect on TAG seasonal variation. The TAG seasonal variation showed that all TAG groups were significantly different between months. The low molecular weight and the medium molecular weight TAG groups increased in winter and decreased in summer, whereas the high molecular weight TAG groups increased in summer and decreased in winter. Based on pooled monthly samples, MALDI-TOF-mass spectrometry allowed the analysis of even- and odd-chain TAG species in MF based on their total carbon number and number of double bonds. These analyses indicated saturated TAG species to be greatest in winter, whereas monounsaturated, polyunsaturated, and odd-chain TAG species were greatest in summer. Our study showed that TAG seasonal variation in a nonseasonal milking system is influenced by the variation in FA composition throughout the seasons.     

Variation in fatty acid composition of ewes' colostrum and mature milk fat

Temporal content variations of approximately 70 C4–C24 fatty acids (FA) in colostrum and milk of ewes consuming winter diet were determined by gas chromatography. The content of monounsaturated, polyunsaturated and straight-chain saturated FA 14:0–16:0 in colostrum was higher whereas the content of 4:0–12:0, 18:0 and branched-chain saturated FA 15:0–17:0 was lower than that in mature milk. The effect of ewe breed on the FA profiles was not significant. The composition of FA changed most significantly 1–2 days after lambing in agreement with the time schedule of colostrum formation. Nevertheless, the content of FA further successively changed up to approximately the 6th day of lactation, whereas further changes up to the 60th day of lactation were smaller. This is consistent with the recommendation that ewes' milk is suitable for human consumption after six-to-eight days of lactation. The higher contents of palmitic and myristic acids in colostrum compared with mature milk suggest that FA composition in colostrum matches the changing needs of the growing lambs.     

Digestibility of silages in relation to their hydroxycinnamic acid content and lignin composition

BACKGROUND: The effectiveness of the analysis of cell wall‐bound hydroxycinnamic acids and the composition of lignin to evaluate the in vivo digestibility of a silage collection with unknown botanical composition was evaluated. RESULTS: Syringyl units content and total etherified phenols showed the highest correlation coefficients with in vivo dry matter digestibility (IVDMD) (r = ? 0.792 and r = ? 0.703, respectively), while guaiacyl units and total phenols showed the highest correlation coefficients with in vivo organic matter digestibility (IVOMD) (r = ? 0.871 and r = ? 0.817, respectively). Using the above‐mentioned chemical parameters, 10 equations were also developed to predict in vivo digestibility. The prediction of IVDMD produced a high adjusted R² value (0.710) using syringyl, total lignin, etherified total phenols, esterified ferulic acid and total phenol content as predictors. The prediction of IVOMD produced a higher adjusted R² value (0.821) using guaiacyl, total phenols, total ferulic acid and etherified p‐coumaric acid content as predictors. CONCLUSION: Cell wall digestibility depends on a multiplicity of factors and it is not possible to attribute a causal effect on in vivo digestibility to any single factor. However, syringyl and guaiacyl content and etherified phenols emerge as good predictors of digestibility. Copyright © 2010 Society of Chemical Industry     

Effect of increasing amounts of a linoleic-rich dietary fat on the fat composition of four pig breeds. Part I: Backfat fatty acid evolution

Four diets prepared, respectively, with 0%, 2%, 4% and 8% of a high-linoleic added fat were administered (76 days of treatment) to a sample of 112 pigs of four breeds (Landrace, Large White, Duroc and a crossbreed Landrace × Duroc). The effects of diet and breed on the evolution of the fatty acid composition of backfat were examined by taking biopsies. Over time, a continuous increase in stearic, palmitic and oleic acids throughout the pig’s life was observed. Oleic acid showed the smallest differences among the four diets at the end of the experiment, while stearic and palmitic acid showed higher differences according to the increase in the percentage of dietary fat. Stearic acid showed the highest rate of increase over time, according to the increasing intake of linoleic acid (diets 1–4). These increases were compensated by a decrease in linoleic acid, although this decrease tended to stabilize according to a higher percentage of added fat and also, for diet 4 (8% fat), an increase in linoleic acid was observed at the end of the experiment. Among the minor fatty acids, arachidonic acid showed a clear decrease over time, although higher levels at the end of the experiment were observed for diets including 4% and 8% of added fat, compared to the other two diets including lower amounts of linoleic acid. Moreover, a significant effect was observed for the factor breed. So, Duroc pigs showed the highest rate of deposit of linoleic acid and the lowest of stearic acid, while the other three breeds showed similar rates.     

The fatty acid composition of Iraqi ewe's, goat's and cow's milk fat

The analysis of the fatty acid composition of 6 samples of the milk fat of each ewes and goats and of 3 samples of the milk fat of cows was accomplished using Hewlett-Packard gas-liquid chromatograph Model 5711 A, provided with an electronic integrator Model 3380. Separation of the methyl esters was performed on columns of 10% sailor on Chromosorb. Temperature programming between 100 degrees C and 180 degrees C was used. The milk fats were qualitatively similar but differed quantitatively. Both ewe's and goat's milk fat had a greater amount of short-chain fatty acids than cow's milk fat. Goat's milk fat contained a higher percentage of short-chain fatty acids compared to that of ewe's milk fat and of goat's milk fat reported in the literature.     

Effect of body fat distribution on the fatty acid composition of the depot fat of cattle

The influence of liveweight, total carcass fat and the amount of fat at five specific anatomical locations on the fatty acid composition of the depot fat of adult ruminants was examined. The total carcassfat and the amount offat at specific locations seem to affect the fatty acid composition significantly. No important significant correlation between liveweight and fatty acid composition was found. There was an increase in odd, even and branched chain fatty acids, and a decrease in linoleic acid, with increase in animal age.     

The relationship between the chemical composition of the carcass and the fatty acid composition of intramuscular fat and backfat of several pig breeds slaughtered at different weights

Pigs of Belgian Landrace (B), Duroc (D), Hampshire (H), and Pietrain (P) breeds were slaughtered at 90, 110 and 130 kg body weight (BW) and the carcass chemical composition and fatty acid profiles of intramuscular fat of loin and backfat were determined. The carcass of Pietrain pigs had a higher concentration of protein and less fat than of B, D and H breeds. In tissues, the PUFA:SFA ratio was lower in the heavier (130 kg BW) than in lighter pigs (90 and 110 kg BW). This feature was higher in P pigs compared with the other breeds. The backfat had a higher concentration of PUFA:SFA ratio than intramuscular fat. The PUFA n− 6:n− 3 ratio was not affected by the breed and weight of pigs, but it was lower in backfat than in longissimus muscle fat. The protein concentration in the carcass was positively related to PUFA and negatively to SFA concentration in tissues.     

Effect of sampling fat location and cooking on fatty acid composition of beef steaks

To investigate the impact of sampling fat location and cooking on fatty acid composition of beef steaks, 21 raw steaks from crossbred steers were dissected to obtain outer (OSC) and inner subcutaneous fat (ISC), seam fat, marbling, and lean muscle. Twenty-one cooked steaks were dissected to obtain OSC, ISC, seam fat, surface and inner muscle. Trans-vaccenic acid and c9, t11-CLA percentages were lower (P < 0.05) in lean muscle than subcutaneous (s.c.) fat or marbling. Monounsaturated: saturated fatty acid ratios were lower (P < 0.05) in seam fat and marbling than s.c. fat or lean muscle. Linoleic and linolenic acid levels were highest in lean muscle and longer chain n−6 and n−3 fatty acids were only detected in lean muscle. Cooking did not change fatty acid composition dramatically except that n−6: n−3 ratio in s.c. and seam fat decreased after cooking (P < 0.05).