External validity and the use of real people as subjects.

A reinforcement effect on participation in group discussion was previously demonstrated in 11 studies using college students as Ss, but was not replicated in the present study with 96 nonstudent volunteers (age range = 17-77 yr.). The difference in results is traced to differences in operant rate for participation between the students and the "real people." Results suggest that the external validity of behavioral research is not primarily a function of the population sampled. Any population is likely to be "atypical" with respect to some behavioral characteristic. Rather, it is suggested that the generalizability of research findings is more a function of the characteristics of the population to which one attempts to generalize. Reliable findings regarding behavioral processes can be accepted as valid no matter what population is sampled in the research, but it should be recognized that the particular characteristics of another population may preclude replication of the phenomenon. (27 ref.) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

New method for studying semantic encoding in children.

Used a familiarization procedure in 2 experiments investigating word encoding in 40 2nd and 40 6th graders. Previous studies using release from proactive inhibition have indicated that developmental changes on some encoding dimensions occur during this period. It is argued that the dependence of release from proactive inhibition on deliberate recall decreases its pertinence to the process of encoding. Exp I confirmed previous findings that the taxonomic classes animals and body parts are encoded by Ss in both grades. Exp II revealed that Ss in both grades also encode the evaluative connotation of words, a finding that has previously emerged only inconsistently. (5 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

A methodology for studying noninstitutionalized psychopaths.

Studies of psychopaths have been limited primarily to institutionalized populations. This article describes a methodological approach to studying noninstitutionalized psychopaths and presents data on various criteria frequently associated with the diagnosis of psychopathy. The procedure involved incorporating the characteristics of psychopathy, in a nonpejorative way, into a major Boston counterculture newspaper ad recruiting Ss for an experiment. Ss were 23 males and 5 females aged 19–47 yrs. They were assessed in several ways using the MMPI, a biographical interview, the Porteus Maze Test, a questionnaire battery (including some Eysenck Personality Inventory scales and measures of empathy, socialization, and Machiavellianism), and a delay of gratification task. Ss fulfilled the criteria for psychopathy, and the recruitment method used appears to be a fruitful one. With the present methodology, it should be possible to determine the extent to which previous results from research can be generalized to nonincarcerated psychopaths and particularly to the more successful ones. (32 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

A method for studying adherence of oral streptococci to solid surfaces

The LDH isoenzyme pattern of the uninvolved mucosa of gastric cancer patients differs as regards the LDH isoenzyme pattern from that of similar tissue of patients with benign gastric disease; the former tissue is characterised by a high M/H ratio of the LDH isoenzymes (M and H sub-units). A high M/H ratio characterises antral mucosa when the latter is compared with fundic mucosa. Mucosa showing superficial and atrophic gastritis also has a higher M/H ratio, whereas the presence of intestinal metaplasia does not appear to influence the M/H ratio. These observations are consistent with the concept that the tissue from which the cancer arose may possess a pre-malignant biochemical lesion.     

Development of a method for studying thematic content of psychotherapy sessions.

The authors developed a measure for categorizing the nature of the topic that clients and therapists discuss from moment to moment during psychotherapy. The Counseling Topic Classification System (CTCS) contains 55 topics and 8 emotional categories. It was used to code the thematic content of Donald Meichenbaum's and Hans Strupp's initial therapy sessions with a client named Richard. Our findings provided preliminary evidence that the CTCS is useful for coding the core topic of initial therapy sessions and that thematic content may be an important process and contextual variable. Methods are described for studying thematic content as a contextual variable and for presenting change process and discovery-oriented research findings so that they are more clinically meaningful. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

A framework for studying personality in the stress process.

This article presents a framework for studying personality in the stress process. The framework specifies that personality may affect both exposure and reactivity to stressful events and that both processes may explain how personality affects health and psychological outcomes. The framework also specifies that personality differences in reactivity may be due to differential choice of coping efforts and differential effectiveness of those efforts. In a 14-day daily diary study of 94 students, this framework was used to analyze the links among neuroticism, daily interpersonal conflicts, coping with conflicts, and distress. Results showed that high-neuroticism participants had greater exposure and reactivity to conflicts. Furthermore, high- and low-neuroticism participants differed both in their choice of coping efforts and in the effectiveness of those efforts, a possibility not considered in previous models of personality in the stress process. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Individual differences in the subjective effects of the first cigarette of the day: A self-report method for studying tolerance.

Some smokers are more sensitive than others to the subjective effects of cigarettes, especially the first cigarette of the day. This report explored self-reported subjective effects to the first cigarette of the day and examined the extent to which heaviness of smoking and years smoking are associated with subjective effects. In 3 independent samples (ns?=?254, 116, 86), self-reports of light-headedness from the first cigarette of the day decreased with increasing heaviness of smoking and increasing number of years smoking, suggesting that differences in responses were due to differences in chronic tolerance. Because measures of the subjective effects of drugs are useful in the study of drug response variability, this self-report item on light-headedness should be included in further research on individual differences in the subjective effects of cigarette smoking. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Informational basis for children's impressions of others.

Assessed the age at which 22 kindergartners, 22 2nd graders, and 22 5th graders used covariation information to form impressions of others when behavioral frequency was held constant or was varied. In Exp I the frequency of aggressive or helpful behavior was held constant, but behavioral consistency or nondistinctiveness was varied. Ss made single-rating and paired comparison judgments about each actor's future behaviors and personal characteristics. Results reveal that 2nd and 5th graders' comparison judgments were appropriately differentiated according to the available covariation information, whereas kindergartners' judgments were not appropriately differentiated. A 2nd experiment was conducted to determine whether 24 kindergartners' failure to use covariation information was simply the result of task demands. Ss made paired comparison judgments about aggressive behavior. Aggression frequency was either held constant (consistency vs nondistinctiveness) or was varied (relative degrees of consistency or nondistinctiveness). Results show that Ss' impressions of others did not vary according to differences in covariation information, at least when frequency was held constant. Findings undermine a task-demand interpretation of the results of Exp I and indicate that young children do use frequency information. There was no evidence that Ss used covariation information independently of differences in frequency. (28 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Comparison of two rule-assessment methodologies for studying cognitive development and knowledge structure.

Compares N. H. Anderson's (1974, 1981) functional measurement methodology and R. S. Siegler's (see record 1977-05568-001) decision tree methodology. Detailed analyses, illustrated with the balance scale paradigm, show that the decision tree methodology is unable to assess algebraic integration rules; instead, it concludes that they are nonintegration, binary decision rules. In contrast, functional measurement proved able to detect both integration and nonintegration rules. It also showed promise for measuring developmental parameters at the individual level. Similar integration–nonintegration issues appear in the choice tasks popular in Piagetian theory and in general decision theory and are discussed in similar terms. (44 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

A method for studying glyoxylic acid induced fluorescence and ultrastructure of monoamine neurons

Inasmuch as precise correlations of light- and electronmicroscopy are crucial for understanding biostructure, it seemed necessary to bring together the advantages of the glyoxylic acid (GA) method (for inducing monoamine fluorescence) and electron microscopy. A combined fluorescence and electron microscope method using GA is introduced. The brain is perfused by 2% GA in Krebs-Ringer bicarbonate buffer (pH 7.0) and this solution is followed by 4% paraformaldehyde containing 0.5% glutaraldehyde in Sorensen's phosphate buffer (pH 7.4). Sections are cut by cryostat or by vitratome and incubated in 2% GA in phosphate buffer (pH 7.0). Using fluorescence microscopy, features of interest are sketched and/or photographed. Afterwards, the same or subsequent section is processed for electron microscopy. Since axons of catecholamine-containing neurons (as well as their perikarya and terminals) are visualized by GA, the recommended procedure expands the range of studies concerning monoamine neurons that can now be carried out effectively.     

A high-risk method for studying psychosocial antecedents of chronic pain: The prospective investigation of herpes zoster.

Although patients with chronic pain (CP) are often psychologically distressed, it has been difficult to determine whether this distress is an antecedent of CP or whether it is caused by the experience of living with CP. This investigation aimed to develop a method that would allow individuals who are at risk for the development of CP to be studied before the pain has become chronic. Patients with acute herpes zoster (HZ) were assessed with demographic, medical, pain, and psychosocial measures. Pain was assessed in follow-up interviews at 6 wks and 3, 5, 8, and 12 mo after these initial assessments. There were no significant differences between Ss who developed short-term HZ pain and Ss who did not develop short-term pain for any of the measures at the initial assessment, except for 1 measure of pain intensity. Ss who developed HZ CP, however, had significantly greater pain intensity, higher state and trait anxiety, greater depression, lower life satisfaction, and greater disease conviction at the initial assessment than Ss who did not develop CP. In discriminant analyses, disease conviction, pain intensity, and state anxiety each made a unique contribution to discriminating Ss who did and did not develop CP. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

A new method for studying the selective adherence of blood lymphocytes to the microvasculature of human retina

PURPOSE: To develop a sensitive and reproducible technique for measuring the adherence of blood lymphocytes to vessel walls exposed in sections of human retina and for examining the role of lymphocyte and vascular adhesion molecules in these events. METHODS: Cryostat sections of human retina were overlaid with blood lymphocytes from healthy subjects, and experimental conditions were sought by which preferential attachment of the cells occurred to blood vessel walls in the retinal sections. Adherent lymphocytes were identified by staining with methyl green-thionine, and transected blood vessels were identified by their structure and by staining of basement membranes with periodic acid-Schiff. The adherence of enriched preparations of CD4+ (T-helper) and CD8+ (T-cytotoxic) lymphocytes, of interleukin-2 (IL-2)-activated cells, and of lymphocytes from patients with ocular Beh?et's disease was examined. The distribution of adhesion molecules on retinal vessel walls was determined by immunohistochemistry, and the contribution of leukocyte integrins to lymphocyte binding was studied by blocking experiments with monoclonal antibodies. RESULTS: The optimal selectivity of blood lymphocyte attachment to retinal vessel walls occurred when purified lymphocytes were suspended in culture medium with 10% fetal calf serum and overlaid onto retinal sections for 30 minutes at 23 degrees C with gentle agitation. Under these conditions, 92% of the lymphocytes that adhered to the section were confined to the retinal microvasculature, and CD4+ T cells were more adherent than CD8+ T cells (P < 0.01). Prior exposure of normal lymphocytes to IL-2 enhanced their binding to retinal blood vessels, and lymphocytes from patients with Beh?et's disease showed supranormal vascular adherence (P < 0.005). Many transected vessels stained positively for CD31; PECAM (mean 62%), CD54; ICAM-1 (mean 73%), CD62E; E-selectin (mean 35%), CD62P; P-selectin (mean 61%), and CD106; VCAM-1 (mean 42%). However, these vascular adhesion molecules occupied < 20% of the area of the blood vessel walls. Lymphocyte adhesion to the retinal vessels was more dependent on CD29 (the common chain of the beta 1 integrins) expression than either CD11a/CD18 or CD49d. CONCLUSIONS: This technique allows measurements to be made of lymphocyte adherence to vascular and nonvascular structures of retina ex vivo. Extension of this approach to the study of leukocyte adherence to sections of pathologic retina may be of clinical and experimental applicability in understanding mechanisms of retinal inflammation.