Egg Thiamine Status of Lake Ontario Salmonines 1995–2004 with Emphasis on Lake Trout

Alewives (Alosa pseudoharengus), the major prey fish for Lake Ontario, contain thiaminase. They are associated with development of a thiamine deficiency in salmonines which greatly increases the potential for developing an early mortality syndrome (EMS). To assess the possible effects of thiamine deficiency on salmonine reproduction we measured egg thiamine concentrations for five species of Lake Ontario salmonines. From this we estimated the proportion of families susceptible to EMS based on whether they were below the ED20, the egg thiamine concentration associated with 20% mortality due to EMS. The ED20s were 1.52, 2.63, and 2.99 nmol/g egg for Chinook salmon (Oncorhynchus tshawytscha), lake trout (Salvelinus namaycush), and coho salmon (Oncorhynchus kisutch), respectively. Based on the proportion of fish having egg thiamine concentrations falling below the ED20, the risk of developing EMS in Lake Ontario was highest for lake trout, followed by coho (O. kisutch), and Chinook salmon, with the least risk for rainbow trout (O. mykiss). For lake trout from western Lake Ontario, mean egg thiamine concentration showed significant annual variability during 1994 to 2003, when the proportion of lake trout at risk of developing EMS based on ED20 ranged between 77 and 100%. Variation in the annual mean egg thiamine concentration for western Lake Ontario lake trout was positively related (p < 0.001, r² = 0.94) with indices of annual adult alewife biomass. While suggesting the possible involvement of density-dependent changes in alewives, the changes are small relative to egg thiamine concentrations when alewife are not part of the diet and are of insufficient magnitude to allow for natural reproduction by lake trout.     

Predation on emergent lake trout fry in Lake Champlain

The rehabilitation of extirpated lake trout (Salvelinus namaycush) in the Great Lakes and Lake Champlain has been hindered by various biological and physiological impediments. Efforts to restore a lake trout fishery to Lake Champlain include hatchery stocking and sea lamprey control. Despite these management actions, there is little evidence of recruitment of naturally-produced fish in annual fall assessments. Spawning occurs at multiple sites lake-wide in Lake Champlain, with extremely high egg and fry densities, yet sampling for juvenile lake trout has only yielded fin-clipped fish. To investigate this recruitment bottleneck, we assessed predation pressure by epi-benthic fish on emergent fry on two spawning reefs and the subsequent survival and dispersal of fry in potential nursery areas. Epi-benthic predators were sampled with 2-h gillnet sets at two small, shallow sites in Lake Champlain throughout the 24-h cycle, with an emphasis on dusk and dawn hours. In total, we documented seven different species that had consumed fry, with consumption rates from 1 to 17 fry per stomach. Rock bass and yellow perch dominated the near-shore fish community and were the most common fry predators. Predator presence and consumption of fry was highest between 19:00 and 07:00. Predators only consumed fry when fry relative abundance was above a threshold of 1 fry trap^− 1 day^− 1. We used an otter trawl to sample for post-emergent fry adjacent to the reef, but did not capture any age-0 lake trout. Due to the observed predation pressure by multiple littoral, species on shallow spawning reefs, lake trout restoration may be more successful at deep, offshore sites.     

Evidence of a remnant self-sustaining strain of lake trout in the Lake Michigan basin

Managers have long embraced the need to maintain diversity as a requisite condition for population and community sustainability. In the case of Great Lakes lake trout, diversity has been severely compromised. The identification of new gamete sources may be beneficial to lake trout reintroduction efforts, particularly in situations where native stocks have been completely extirpated such as in Lake Michigan. Lake trout from Elk Lake, Michigan, are genetically distinct from domestic hatchery strains and historical forms of lake trout from Lake Michigan. Importantly, Elk Lake fish were genetically distinct from Marquette strain lake trout which were previously stocked into Elk Lake. Elk Lake fish were most similar to Lake Michigan basin-derived Lewis Lake (LLW) and Green Lake (GLW) hatchery strains and to historical Lake Michigan populations from the Charlevoix, Michigan area. While all individuals exhibited characteristics of lean form lake trout, the body shape of lake trout from Elk Lake, stocked lean fish from Lake Michigan and Lake Superior wild lean strains from near Isle Royale differed. Elk Lake fish were more fusiform, elongate, and streamlined with a narrower caudal peduncle compared to hatchery lean strains and wild lean forms from the Isle Royale region of Lake Superior. The lake trout population in Elk Lake is a remnant of a now extirpated native Lake Michigan population that was established either by natural colonization or stocking from historical Lake Michigan populations. Elk Lake lake trout is as genetically diverse as other strains used in Great Lakes reintroduction efforts and likely represent a viable gamete source representing genetic diversity lost from Lake Michigan.     

Contribution of lake trout stocked as fry to an adult population in Lake Superior

Lake trout Salvelinus namaycush fry treated with heated water to create thermal marks in their otoliths were stocked at Sve's Reef in Minnesota waters of Lake Superior in 1994, 1995, and 1996. These fish began to reach maturity in 2000, and were vulnerable to annual assessment gill nets set at several locations along the Minnesota shoreline. Captured fish also included fin-clipped lake trout stocked as yearlings, and naturally reproduced (wild) lake trout. Otoliths from 3106 unclipped lake trout were aged and examined for thermal marks from 2000 to 2007, of which 1152 were from the target year classes (1994–1996). Thermal marks were found in otoliths from 64 fish, or 5.6% of those in the target year classes, demonstrating that stocked fry contributed to the adult lake trout population in Minnesota waters. Although numbers of recaptured fish were too low to demonstrate statistically significant differences, higher recapture rates of marked fish at Sve's Reef in fall and spawning assessments suggest that these fish may have imprinted at the stocking location and homed back to this area to spawn. Wild lake trout populations in Lake Superior may be approaching fully rehabilitated levels, but recovery in the lower Great Lakes has progressed more slowly, and evidence of success with fry stocking could benefit those populations.     

Foraging mechanisms of age-0 lake trout (Salvelinus namaycush)

Reaction distances under various light intensities (0-19 uE/m2/s), angles of attack, swimming speeds, and percentage of overall foraging success were measured. Extensive efforts have been invested in restoring lean lake trout (Salvelinus namaycush) populations in the Laurentian Great Lakes, but successful natural recruitment of lake trout continues to be rare outside of Lake Superior and parts of Lake Huron. There is evidence of high mortality during the first several months after eggs hatch in the spring, but little is known about the foraging mechanisms of this age-0 life stage. We developed a foraging model for age-0 lake trout (S. namaycush) in response to amphipods (Hyalella azteca) and mysids (Mysis diluviana) by simulating underwater environmental conditions in the Great Lakes using a temperature-controlled chamber and spectrally matched lighting. Reaction distances under various light intensities (0–19 uE/m²/s), angles of attack, swimming speeds, and percentage of overall foraging success were measured. Intake rates under different light intensities and prey densities were also measured. Age-0 lake trout were non-responsive in the dark, but were equally responsive under all light levels tested. Age-0 lake trout also demonstrated a longer reaction distance in response to moving prey, particularly mysids, which had an escape response that reduced overall foraging success. We determined that prey intake rate (numeric or biomass) could be modeled most accurately as a function of prey density using a Michaelis–Menton equation and that even under low mysid densities (3 individuals/m²), age-0 lake trout could quickly satisfy their energetic demands in a benthic setting.     

Thiamine concentrations in lake whitefish eggs from the upper Great Lakes are related to maternal diet

Thiamine deficiency is responsible for reproductive impairment in several species of salmonines in the Great lakes, and is thought to be caused by the consumption of prey containing thiaminase, a thiamine-degrading enzyme. Because thiaminase levels are extremely high in dreissenid mussels, fish that prey on them may be susceptible to thiamine deficiency. We determined thiamine concentrations in lake whitefish Coregonus clupeaformis eggs from the upper Laurentian Great Lakes to assess the potential for thiamine deficiency and to determine if thiamine concentrations in lake whitefish eggs were related to maternal diet. Mean thiamine concentrations in lake whitefish eggs were highest in Lake Huron, intermediate in Lake Superior, and lowest in Lake Michigan. Some fish had thiamine concentrations below putative thresholds for lethal and sublethal effects in salmonines, suggesting that some larval lake whitefish may currently be at risk of at least sublethal effects of low thiamine concentrations, although thiamine thresholds are unknown for lake whitefish. Egg thiamine concentrations in lake whitefish eggs were statistically significantly related to isotopic carbon signatures, suggesting that egg thiamine levels were related to maternal diet, but low egg thiamine concentrations did not appear to be associated with a diet of dreissenids. Egg thiamine concentrations were not statistically significantly related to multifunction oxidase induction, suggesting that lower egg thiamine concentrations in lake whitefish were not related to contaminant exposure.     

Evaluation of the thiamine dose-response relationship for lake trout (Salvelinus namaycush) fry using an individual based model

Substantial natural reproduction of lake trout (Salvelinus namaycush) has not been achieved in the Great Lakes, except for Lake Superior and a few areas in Lake Huron, despite continued stocking efforts. Low thiamine levels in lake trout eggs, which can result in lethal and sublethal impacts (thiamine deficiency complex, TDC) on fry, may contribute to widespread recruitment failure in lake trout populations. We hypothesized that incorporation of sublethal impacts into dose-response curves would result in estimates of EC50s (median lethal concentrations) for fry greater than the estimates that rely only on acute mortality and that predation would exacerbate thiamine effects. To investigate the sublethal effects of TDC (prey capture success and predation mortality) on cohort growth and survival, we developed an individual-based model for lake trout fry. The model tracks daily activities, including consumption, respiration, growth, and mortality, of lake trout from hatch until fry reach a length of 33?mm when we assume fry feed naturally and thiamine effects are minimized. Model output with sublethal impacts resulted in an EC50 (7.3?nmol/g) that was greater than published studies that are limited to acute mortality (1.5?nmol/g). Furthermore, when we included interstitial and pelagic predation, the impact of sublethal effects shifted the EC50 values even higher (7.4–10?nmol/g). Simulation results indicate that low thiamine levels, in combination with moderate to high predation, can eliminate lake trout cohorts. Our simulations suggest that the sublethal effects of low thiamine can contribute to poor lake trout recruitment more than previously suspected.     

Heritage strain and diet of wild young of year and yearling lake trout in the main basin of Lake Huron

Restoration of lake trout Salvelinus namaycush stocks in Lake Huron is a fish community objective developed to promote sustainable fish communities in the lake. Between 1985 and 2004, 12.65 million lake trout were stocked into Lake Huron representing eight different genetic strains. Collections of bona fide wild fish in USGS surveys have increased in recent years and this study examined the ancestry and diet of fish collected between 2004 and 2006 to explore the ecological role they occupy in Lake Huron. Analysis of microsatellite DNA revealed that both pure strain and inter-strain hybrids were observed, and the majority of fish were classified as Seneca Lake strain or Seneca Lake hybrids. Diets of 50 wild age-0 lake trout were examined. Mysis, chironomids, and zooplankton were common prey items of wild age-0 lake trout. These results indicate that stocked fish are successfully reproducing in Lake Huron indicating a level of restoration success. However, continued changes to the benthic macroinvertebrate community, particularly declines of Mysis, may limit growth and survival of wild fish and hinder restoration efforts.     

Evidence of lake trout (Salvelinus namaycush) spawning and spawning habitat use in the Dog River,Lake Superior

Lake trout spawn primarily in lakes, and the few river-spawning populations that were known in Lake Superior were believed to be extirpated. We confirmed spawning by lake trout in the Dog River, Ontario, during 2013–2016 by the collection of and genetic identification of eggs, and we describe spawning meso- and microhabitat use by spawning fish. Between 2013 and 2016, a total of 277 lake trout eggs were collected from 39 of 137 sampling locations in the river. The majority of eggs (220) were collected at the transition between the estuary and the river channel crossing the beach. Lake trout eggs were most often located near the downstream end of pools in areas characterized by rapid changes in depth or slope, coarse substrates, and increased water velocities, where interstitial flows may occur. Depths in wadeable areas where eggs were found averaged 0.9?m (range: 0.4 to 1.3?m) and substrate sizes consisted of large gravel, cobble, and boulder; comparable to spawning characteristics noted in lakes. Water velocities averaged 0.66?m·s^?1 (range: 0.33 to 1.7?m³·s^?1) at mid-depth. This information on spawning habitat could be used to help locate other remnant river-spawning populations and to restore river-spawning lake trout and their habitat in rivers that previously supported lake trout in Lake Superior. The Dog River population offers a unique opportunity to understand the ecology of a river spawning lake trout population.     

Estimating wounding of lake trout by sea lamprey in the upper Great Lakes: Allowing for changing size-specific patterns

A primary fishery concern in the Laurentian Great Lakes is mitigating the persistent negative impact of parasitic sea lamprey (Petromyzon marinus) on native lake trout (Salvelinus namaycush). Wounds observed on surviving lake trout are commonly used by managers to assess damages associated with sea lamprey predation. We estimated the relationship between wounding rates and lake trout size, and how this varied spatially and temporally. We built upon previously published work by fitting wound rates as a logistic function of lake trout size. By using longer time series and data from three Great Lakes, our analysis harnessed substantially more contrast in host populations than previous work, and we also employed software advances for nonlinear mixed-effect models. Candidate models allowed logistic function parameters to be constant or to vary spatially, temporally, or both. Temporal effects were modeled as random walk processes. We also considered models that assumed either Poisson or negative binomial distributions for the number of wounds per fish at a given length. Models that allowed for both spatial and temporal effects in the shaping parameters and assumed a negative binomial wound distribution resulted in the best fit as indicated by Akaike's Information Criterion. Wounding rate estimates from models selected for each lake in this analysis are contrasted with those of wounding rate models currently used as components of lake trout population assessments. Although model fit was improved substantially, differences in wounding rates estimates are modest and estimates follow very similar temporal trends. However, as time series of wound data continue to grow, models that incorporate temporal variability in parameter estimation are expected to be increasingly favored. This research provides managers with an updated tool to obtain more reliable estimates of sea lamprey wounding.     

A hydrodynamics-based framework to evaluate the impact of fishways on drifting lake sturgeon larvae

Lake sturgeon (Acipenser fulvescens) have recently been a target for conservation in the Laurentian Great Lakes. While improving spawning success has been a major goal of these efforts, an often-overlooked component is the survival of the larvae after hatching, during the period of downstream drift. In a dammed river system, during this phase, larvae may need to drift past dam infrastructure. This journey past dams often results in an increase in larval mortality for a variety of reasons, including exposure to highly turbulent flow. Quantifying the aspects of turbulence related to larval mortality within fishways will inform retrofitting or future design efforts of fishways to improve larval viability. This study uses dimensional arguments to characterize the flow conditions influencing larval viability through fishways. One such condition discussed here is strain rate, which can be used as a diagnostic basis to determine candidate fishways for conservation measures. Based on Kolmogorov’s theory (1941), the strain rate present in the fishway at the pertinent scale for lake sturgeon larvae, S_η, can be estimated using the fishway’s macroscale Reynolds number Re, the relevant macroscale fishway velocity U, and the smallest fishway pool dimension l_e as S_ηl_e/U ≈ Re^1/2. This approach is illustrated in the case of the Vianney-Legendre Fishway in Québec, and determined this fishway to be potentially hazardous to drifting lake sturgeon larvae.     

Evaluation of adult and offspring thiamine deficiency in salmonine species from Lake Ontario

Thiamine Deficiency Complex (TDC) is an ongoing problem impacting salmonine health in various waterbodies, including Lake Ontario. The prevalence of TDC has been variable and explanations for differences are limited. In the current study, thiamine concentrations were measured in eggs, liver tissue, and muscle tissue sampled from brown trout (Salmo trutta), Chinook salmon (Oncorhynchus tshawytscha), coho salmon (O. kisutch), lake trout (Salvelinus namaycush), and steelhead trout (O. mykiss) that were collected from Lake Ontario and its surrounding tributaries. The occurrence of TDC was measured for each species based on TDC-induced offspring mortality rates under laboratory conditions. TDC-induced offspring mortality was observed for all species except brown trout. For affected species, egg free thiamine (Th) was consistently low compared to lake trout collected from Lake Superior that are considered thiamine replete. In addition, species with the lowest percentages of Th in their eggs were the most susceptible to TDC, suggesting that limited thiamine reserves in the form of Th may cause TDC-induced offspring mortality. Lastly, our results show that egg thiamine concentrations have yearly variation and increased for all species throughout the study. Reasons for such variation are undetermined; but, if egg thiamine concentrations continue to increase, the impacts of TDC on these salmonine species may lessen. Future monitoring is needed for determining if thiamine concentrations are increasing and the potential impacts that may have on the entire Lake Ontario fishery.     

Genetic estimates of jurisdictional and strain contributions to the northeastern Lake Michigan brown trout sportfishing harvest

The Lake Michigan brown trout (Salmo trutta) fishery is sustained by the stocking of five hatchery strains by four state natural resource agencies. In the absence of exhaustive marking programs, strain-specific measures of stocking success are lacking for brown trout in Lake Michigan. We used microsatellite-based genetic assignment testing and genetic stock identification (GSI) to determine the strain of 122 angler-caught brown trout from four northeastern Lake Michigan ports. We compared strain composition estimates for sportfishing harvest to expected proportions of each brown trout strain in Lake Michigan at the time of harvest using stocking records corrected for age-specific mortality rates. Reassignment rates of individuals from baseline strains averaged 92.1% (range: 84.1–98.0%). Assignment testing and GSI analyses consistently found Wild Rose strain brown trout represented approximately 89% of the northeastern Lake Michigan sportfishing harvest, while only comprising 43.8% of the expected stock. Of the Michigan angler harvest of Wild Rose strain brown trout, approximately half were estimated to have originated from Wisconsin hatcheries, demonstrating a propensity for lake-wide movements. Continued assessments will improve understanding of strain relative contributions to angler harvests that can direct future stocking efforts.     

美洲红点鲑鱼种养殖技术模式研究

作者通对美洲红点鲑(Salvelinus fontinalis)苗种3种不同养殖密度试验研究,300尾/m2,600尾/m2,400尾/m2,在本次试验条件下最佳养殖效果为600尾/m2,成活率90%,平均体重47.6g/尾,产量27.7kg/m2,饲料系数1.18,提出了美洲红点鲑规模化养殖条件下最佳养殖模式。     

A model to predict embryonic development and hatching in lake whitefish (Coregonus clupeaformis) under variable incubation temperatures

We developed an incremental growth-at-temperature model to predict hatch timing for lake whitefish under varying incubation temperatures. The model extends earlier approaches by incorporating a temperature-dependence for development stage at hatching. Testing using experimentally reared embryos from Lakes Huron and Simcoe demonstrates improved predictive accuracy compared to previous models for asymmetrically varying thermal regimes that mimic aspects of those encountered under real-world conditions. The simple calibration of the model to different populations and its ability to predict observed phenomena such as thermally-induced mass hatching and hatching synchronization makes it a useful tool for the evaluation of thermal impacts from industrial discharges and climate change.     

Migratory behaviour of adult fast-growing brown trout (Salmo trutta,L.) In relation to water flow in a regulated Norwegian river

The migration pattern of spawners of brown trout (Salmo trutta) in relation to water flow was analysed by radio-tracking in the regulated River Gudbrandsdalslågen, Norway. During the upstream spawning migration in the high flow period, trout (2–12 kg) were caught, tagged and released 15 km downstream of a fish ladder at the Hunderfossen waterfall. The released fish displayed a systematic and directional upstream movement to the outlet of the tunnel from the hydroelectricity plant. In autumn and winter there is a minimum water discharge of 20–2 m³/s on p.s. in the river between the dam and the outlet of the tunnel, which has a discharge of 200–300 m³/s. When the water flowing over the Hunderfossen dam decreased to 20 m³/s, the ascent of brown trout up the river stopped and fish periodically entered the power plant tunnel. To determine the flow necessary to attract fish into the spawning reach above the tunnel outlet, two experiments were undertaken using 12 and 17 radio-tagged trout. In the first experiment, 60 m³/s of water released for 24 h resulted in the migration of 50% of the trout up the river. The second experiment, releasing 60 m³/s for 24 h, followed by 30 m³/s for 24 h two days later, resulted in the migration of 60% of the trout. Only one fish ascended the river at a flow of 30 m³/s. It is recommended that a repeated release of water at 60 m³/s is made in periods of minimum water discharge to save the spawning migration. The results demonstrate the advantage of using radio-tracking in experiments dealing with fish migration in relation to water-flow management.     

Mass balance analysis and calculation of wind effects on heat fluxes and water temperature in a large lake

Modeling applications often ignore the water mass balance for hydrodynamic simulations in large lakes such as the Great Lakes. However, evaluation of the water budget can be of interest, especially when water surface calculations are important, and also as it relates to the heat budget. In this study Lake Ontario is modeled using the Environmental Fluid Dynamics Code for the heating seasons in 2008–2013, with calibration focused on 2008, for which more data are available. The model is used to test both water mass and heat budgets, and various interpolation schemes for meteorological data are tested to determine the degree of sensitivity of results to the interpolation method used. The water budget incorporates flow from major tributaries and precipitation/evaporation, and it is shown that these processes alone cannot provide a balance. Missing water quantities include direct runoff and flows from smaller, probably ungauged tributaries. In 2008 the calculated quantity is 1.61?×?10¹⁰?m³ during April to September, which is equivalent to a change in water surface level of 0.7?m and represents an average flow rate of 1018?m³/s, which is larger than the inflow of any tributary to the lake except the Niagara River. Latent and sensible heat flux calculations are highly affected by wind, and interpolation of wind data is found to impact model calculations of those quantities, though different interpolation schemes do not have a strong effect on surface water temperatures. In general, the natural neighbor interpolation method is preferred over inverse distance methods.     

Effects of transferring glacier-fed water to a clear-water mountain river on the production and food organisms of brown trout (Salmo trutta L.) in Southern Norway

The effects of transferring glacier-fed water to a clear-water river on production and food organisms of brown trout (Salmo trutta L.) were investigated in a mountain river, in southern Norway. Production in unregulated fluvial habitat was 271.5 g 100 m^?2 yr^?1 compared with 103.1 g 100 m^?2 yr^?1 in a glacier-fed reach of the river. This difference was due to low fish density and recruitment rate. The growth rate, at least for younger fish (2+ to 4+ age groups), was significantly higher in the regulated reach of the river. The main difference in food composition was the low abundance of crustaceans Eurycercus lamellatus and Gammarus lacustris in the regulated reach. Trichopterans were the main diet component in both sites.     

Contemporary diets of Lake Superior lake whitefish off the Keweenaw Peninsula and changes in condition from the 1980s to 2010s

Over the last two decades, declines in lake whitefish (Coregonus clupeaformis) recruitment and growth in many areas of the Laurentian Great Lakes have raised concerns about the status of this important species. Although Lake Superior populations have been less affected than those in other Great Lakes, these populations still face multiple threats. We characterized lake whitefish diets collected off the Keweenaw Peninsula between 2015 and 2017 and compared results to previous Lake Superior studies. We additionally estimated length-weight relationships to determine whether lake whitefish body condition (i.e., expected weight-at-length) had changed since the 1980s. Diet diversity was low, although individual specialization was moderate to high. Fish transitioned from consuming Diporeia in the spring to Mysis and fish eggs during fall and winter; sphaeriids composed 20–30% of diets across all seasons. Compared to findings for other Lake Superior regions, lake whitefish diets comprised lower percentages of high energy items (e.g., Diporeia, Mysis) and higher percentages of low energy items (e.g., sphaeriids). Expected weights in the 2000s and 2010s were lower in the 400- and 500-mm length groups but similar in larger lengths groups compared to the 1980s; condition was highest across all lengths in the 1990s. The observed decline in condition since the 1990s in the 400- and 500-mm length groups, in combination with possibly greater consumption of less energetically profitable items, suggests that lake whitefish <600 mm or preferred prey resources in this lake region may be experiencing stressors leading to condition declines, although what these stressors are remain unknown.     

Challenges to collective action in the management of the Kapenta fishery in lake Kariba

This study focused on the Kapenta fishing industry at Lake Kariba to illustrate that variations in economic situation, and in biophysical attributes of the resource users, make it difficult to institute collective conformity with fishery management regulations. The paper discusses how the Kapenta fishing companies in the Lake Kariba region have differing preferences regarding fishery resource management, and assign different priorities to various objectives of the resource management. The differences in personal objectives regarding management of the fishery resources, and the differences in the degree of control and access over the fishery, led to situations where users had varying incentive structures. The differences in incentive structures lead to fishery management regulations based on the average characteristics of users and the biophysical environment, fuelling conflicts between fishery resource managers and users. The variations of micro‐biophysical attributes of the resource, combined with the behaviour of the resource users, make it difficult to bring resource users together for developing and initiating collective action.