共查询到11条相似文献,搜索用时 46 毫秒
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采用双相氯胺-T法对聚乙二醇化重组人白细胞介素-6(PEG-rhIL-6)进行碘化标记,比较了双相氯胺-T法碘化标记PEG-rhIL-6在不同反应条件下的标记效果.用三氯乙酸沉淀法和γ计数探测仪测定标记物的标记率、超滤离心法对标记物进行分离纯化、三氯乙酸沉淀法鉴定标记物的放化纯度、SDS-PAGE电泳分析标记蛋白断链损伤情况.结果表明,采用该方法标记PEG-rhIL-6,在100μL、pH值7.4的反应体系中,控制反应蛋白量为25μg、浓度20g/L的氧化剂15μL、室温反应时间40-50 min,可获得较高的标记率和放化纯度,比放射性适中,标记效果比较理想. 相似文献
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Ali Akbar ASHKARRAN 《等离子体科学和技术》2013,15(4):376-381
We report studies on the growth of gold nanoparticles by a seed-mediated approach in solution. The synthetic method is adapted from one we published earlier (Ashkarran et al. Appl. Phys. A 2009, 96, 423). The synthesized gold nanoparticles were characterized by X-ray diffraction (XRD), dynamic light scattering (DLS), UV-Vis spectroscopy, optical imaging and atomic force microscopy (AFM). Optical absorption spectroscopy of the prepared samples at 15 A arc current in HAuCl 4 solution shows a surface plasmon resonance around 520 nm. It is found that sodium citrate acts as a stabilizer and surface capping agent of the colloidal nanoparticles. The intensity of the plasmonic peak of the prepared gold nanoparticles for 1 minute arc duration gradually increases due to seed mediation for up to 6 hours. The formation time of gold nanoparticles at higher seed concentrations is less than that at lower seed concentrations. 相似文献
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摘要:本文对血管内皮细胞生长因子(VEGF)的结构、功能及生理作用,VEGFR的靶向抗肿瘤新生血管生成治疗,VEGF的PET示踪剂分类,采用18F标记的VEGFR酪氨酸激酶抑制剂类合成等几个方面做了简要叙述。 相似文献
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The application of keV ion beam in life science started in China several decades ago. In 1986, researchers initially studied the mutagenic effect of ion beam, and successfully applied it to plant breeding. Nowadays, ion beam implantation technique has been extensively applied to many biological fields. This paper mainly introduces one of its important applications: genetic transformation mediated by keV ion beam. 相似文献
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《中国原子能科学研究院年报(英文版)》2001,(1)
The novel coupling method based on the hyphenation of immunoreaction with ICP-MS is developed and applied to the determination of thyroid-stimulating hormone (TSH) in human serum. In this system, TSH is firstly captured by anti-TSH monoclonal antibodies immobilized on a solid support. Biotinylated anti-TSH monoclonal antibodies and Eu3 -labelled streptavidin are then added to form the complex capturing andbody-TSH-biotinylated anti-TSH antibody-Eu3 -labeled streptavidin. After unbound reactants are washed away, Eu3 bound to the complex is extracted by 相似文献
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采用亲核反应制备~(18)F-Fallypride。将不同浓度的~(19)F-Fallypride与~(18)F-Fallypride混匀模拟比活度变化,经尾静脉注射正常小鼠(3.7 MBq)后10min行Micro PET显像,勾画纹状体为感兴趣区域,计算其摄取~(18)F-Fallypride的变化。结果表明,各组纹状体摄取~(18)F-Fallypride的量随比活度降低而降低,摄取值与19F-Fallypride的浓度对数呈S型曲线变化。~(18)F-Fallypride的比活度不低于1.35PBq/mol时,可保证~(18)F-Fallypride PET显像的准确性。结果可见,~(18)F-Fallypride比活度是多巴胺D2受体显像的重要影响因素。 相似文献
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肝细胞受体显像剂99mTc-GSA的制备及其药盒化 总被引:3,自引:2,他引:1
通过DTPA环酐,将双功能连接剂引入人血白蛋白(HSA)分子;再通过合成2-亚氨基-2-甲氧基乙基-1-硫代-β-D-半乳吡喃糖苷,与DTPA-HSA反应,引入硫代半乳糖残基,得到GSA。以氯化亚锡为还原剂,通过标记条件的摸索成功制备了标记率大于96%的标记配合物99mTc-GSA。为方便该药物的临床研究以及应用推广,进一步研制了无菌的GSA一步法冻干药盒,并对湿法以及冻干法制备的该标记配合物进行了比较。在正常小鼠以及肝损伤模型中的分布试验表明,99mTc-GSA在正常小鼠肝脏中有较高的摄取,在30min时,肝脏摄取仍大于70%ID•g-1,且具有饱和性;在肝损伤模型中肝摄取值低于正常小鼠(P=0.0324)。药盒法标记所得生物数据与湿法标记相当。所得GSA一步法冻干药盒标记简单可靠,有优良的生物性能,可提供临床进一步研究、应用。 相似文献