Tissue engineering has emerged as an alternative treatment to traditional grafts for skin wound healing. Three-dimensional nanofibers have been used extensively for this purpose due to their excellent biomedical-related properties. In this study, high porous 3D poly lactic acid nanofibrous scaffolds (PLA-S) were prepared by wet-electrospinning technique and seeded with rat bone-marrow stem cells (BMSCs) to characterize the biocompatibility and therapeutic efficacy of these fibers on the treating full-thickness dermal wounds. The results of in vitro andin vivo studies indicate that the 3D fibrous PLA-S can be a potential wound dressing for wound repair, particularly when seeded with BMSCs. 相似文献
Bone tissue scaffolds should have both desired mechanical stability and cell activities including biocompatibility, cell differentiation, and maturation. Also, suitable mineralization is another key factor for these materials. Hence, in current work, in order to achieve a scaffold with desired mechanical and bioactivity properties, core-shell nanofibers based on the polycaprolactone and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with different concentration of graphene oxide (GO) (0.5, 1, and 1.5 wt%) and calcium phosphate (CP) (1 and 3 wt%) were prepared to utilize as bone scaffold. Microstructure of nanofibers observed by field emission scanning electron microscope (FE-SEM) and results exhibited that the most of nanofibers had 270–500 nm diameter. Attenuated total reflectance Fourier transform infrared spectroscopy and energy dispersive X-ray evaluations verified appearance of GO and CP into the electrospun scaffolds (ES). Transmission electron microscopy analysis endorsed core-shell structure of nanofibers. X-ray diffraction study moreover determination of semicrystalline structure, verified presence of GO and CaPO4 into the nanofibers. Water contact angle demonstrates that, ES2 and ES3 situated in suitable domain of hydrophilicity. Tensile analysis determined that, ES2, ES3, and ES4 had the highest mechanical properties for use as bone scaffold. Cell viability assessment confirmed biocompatibility of scaffold during 7 days. Alkaline phosphatase and alizarin red staining exhibited maturating and differentiating of osteocytes after 21 days seeding on the scaffolds. 相似文献
Summary: Biomimetic scaffolds are appealing products for the repair of bone defects using tissue engineering strategies. The present study prepared novel biomimetic composite scaffolds with similar composite to natural bone using bioactive glass, collagen, hyaluronic acid, and phosphatidylserine. The microstructure, swelling ratio, biodegradability, and biomineralization characteristic of the composite scaffolds with and without hyaluronic acid and phosphatidylserine were compared and analyzed by SEM/EDAX, XRD, and FTIR techniques and in vitro test, and the properties can be influenced by 1‐ethyl‐3‐(3‐dimethylaminopropyl) carbodiimide (EDC)/N‐hydroxysuccinimide (NHS) crosslinking. The optimized properties of the crosslinked composite scaffolds observed in this study show the possibility of their use of bioactive and bioresorbable scaffolds in bone tissue engineering.
SEM micrographs of BG‐COL‐HYA‐PS composite scaffolds after immersion in SBF for 1 d. 相似文献
