Influence of modified blanching treatments on inactivation of Salmonella during drying and storage of carrot slices

Documented outbreaks of human illness associated with consumption of minimally processed produce have increased in recent years. This study evaluated the influence of modified treatments on inactivation of Salmonella during preparation, home-type dehydration (60 degrees C, 6h) and storage of carrot slices. Inoculated (five strains, 7.8 log cfu/g) slices were subjected to the following treatments: (i) untreated control, (ii) steam blanching (88 degrees C, 10 min), (iii) water blanching (88 degrees C, 4 min), (iv) blanching in a 0.105% citric acid solution (88 degrees C, 4 min), or (v) blanching in a 0.21% citric acid solution (88 degrees C, 4 min), dried for 6h at 60 degrees C (140 degrees F), and stored for up to 30 d. Bacterial populations were reduced by 3.8-4.1, 4.6-5.1 and 4.2-4.6 log cfu/g immediately following steam, water or citric acid blanching, respectively. After 6h of dehydration, total reductions were 1.6-1.7 (control), 4.0-5.0 (steam blanched), 4.1-4.6 (water blanched) and 4.9-5.4 (blanched in citric acid solution) log cfu/g. Populations continued to decrease throughout storage, but were still detectable by direct plating at 30 d on all samples except for those blanched in 0.21% citric acid. Results suggest that blanching carrot slices, particularly blanching in 0.21% citric acid, before drying should enhance inactivation of Salmonella during home-type dehydration and storage.     

Effect of blanching and drying treatments on quality of bamboo shoot slices

The enzymes activity, texture, pectin, microstructure and colour change in bamboo shoots (Dendrocalamus latiflorus) were examined to evaluate the effect of blanching and drying treatments on quality of bamboo shoot slices (BSS). Peroxidase (POD), phenylalanine ammonia‐lyase and polyphenol oxidase in BSS will become completely inactive at 95 °C for 6‐, 9‐ and 12‐min blanching treatments, respectively. The hardness and protopectin (PP) content decreased after the blanching treatment. Meanwhile, the content of water‐soluble pectin increased initially and then decreased gradually during the blanching. After the blanching treatment, the L* and a* of BSS decreased, while b* increased, ΔE > 2. The hardness of rehydrated BSS decreased by 43.57% after freeze drying (FD), which, however, decreased more after hot air drying (HAD). The pectin of rehydrated BSS was also determined to explain the hardness change in BSS. The BSS acquired similar microstructure of fresh samples after FD, while microstructure of BSS was severely damaged after HAD. The colour of BSS changes significantly after drying treatment. However, FD can maintain better colour of BSS than HAD. The results may provide a reference for industrial production of BSS.     

不同热烫处理对马铃薯片中还原糖浓度的影响研究

以还原糖含量为评价指标,还原糖的含量通过DNS法测得,旨在研究不同热烫处理在降低马铃薯片中还原糖含量方面的作用,从而抑制切片型薯片油炸过程中致癌物质丙烯酰胺的生成。在单因素实验的基础上进行正交实验,考察热烫温度和时间两个因素在降低还原糖含量方面的作用。结果表明,热烫处理的最佳工艺条件为：热烫温度75℃、热烫时间135s。     

The effect of blanching on the mechanical and rehydration properties of dried potato slices

 Short-time blanched (2 min, 90  °C), long-time blanched (30 min, 90  °C) and non-blanched potato slices were dried in a convective air drier and their mechanical and rehydration properties were compared. Blanching increased the flexibility and strength of dried potato slices, although the effects of short and long blanching were not significantly different. Unblanched potato slices did not have larger rehydration ratios than blanched ones. After rehydration for 30 min, samples from all treatments had higher strength and flexibility than cooked potatoes. Received: 2 November 1998 / Revised version: 15 February 1999     

Starch gelatinization temperatures within potato during blanching

The progression of gelatinization within potato cylinders blanched at 65,75 and 85°C for 2, 4 and 6 min was followed by iodine staining of sections. The distance to the gelatinization front was measured on photographs of both blanched and stained potatoes. A time-temperature simulation was performed with a finite difference computer program using thermal conductivity, specific heat, phase transition, density, heat-transfer coefficient, and initial temperatures as input data. Measured distances were used to determine the simulated temperature at the gelatinization front. The computer-simulated temperatures used to determine the gelatinization temperature within blanched potato were in good agreement with published values.     

Inactivation of Salmonella during drying and storage of Roma tomatoes exposed to predrying treatments including peeling, blanching, and dipping in organic acid solutions

The objective of this study was to evaluate the influence of predrying treatments, i.e., peeling, blanching prior to inoculation, and dipping in organic acid solutions, on inactivation of Salmonella during drying (60 degrees C for 14 h) and aerobic storage (25 degrees C for 28 days) of inoculated (five-strain composite, 7.1 to 7.4 log CFU/g) Roma tomato halves. Four predrying treatments groups were established. One group received no treatment (C). In the other three groups, unpeeled-unblanched, unpeeled-blanched (steam blanched at 88 degrees C for 3 min), peeled-unblanched, and peeled-blanched tomato halves were immersed for 10 min in water (W), ascorbic acid solution (AA; 3.40%, pH 2.48), or citric acid solution (CA; 0.21%, pH 2.51). Appropriate dilutions of homogenized tomato samples were spread plated on tryptic soy agar with 0.1% pyruvate and XLT4 agar for bacterial enumeration during drying and storage. Ten minutes of immersion in W, AA, or CA reduced bacterial populations by 0.7 to 1.6 log CFU/g. After 14 h of dehydration, total log reductions in the populations of bacteria were 3.2 to 4.5 (C), 3.7 to 4.9 (W), > 5.6 to > 6.1 (AA), and 4.5 to 5.5 (CA) log CFU/g, depending on type of agar used and condition of tomato samples. During drying and storage, the order of pathogen inactivation for predrying dipping treatments was AA > CA > W > C, with AA and CA rendering bacterial populations below detectable levels ( < 1.3 log CFU/g) prior to storage and between 7 and 14 days of storage, respectively. The results also indicated that peeling and blanching of tomatoes prior to inoculation may not necessarily affect destruction of Salmonella during the drying process. Use of predrying acid dipping treatments of tomatoes, especially in AA, may improve destruction of Salmonella during the dehydration process.     

Modelling the effect of different sterilisation treatments on antioxidant activity and colour of carrot slices during storage

The effect of F₀ treatment time (min) and storage on the antioxidant activity and Hunter colour parameters (L*, a*, b*) of carrot slices was investigated. Carrot slices were sterilised for 0 (control), 3, 15 and 50 min and subsequently stored for 0, 3 and 6 months. Significant differences were observed in colour values of carrot slices with no significant difference beyond F₀ treatment of 3 min. Regression modelling was used to investigate the main effects of treatment time and storage. Treatment time and storage period was found to be significant. Predicted models were found to be significant (p < 0.05) with low standard error and high coefficients of determination (R²). This study proposes the predicted models for quality parameters of sterilised carrot slices.     

Influence of process variables on the drying of potato slices

Investigation of the effects of varying air velocity, slice thickness, and pre-treatment with sodium chloride solutions and surface active agents on drying potato slices indicated that the drying occurred entirely in the falling rate period and was controlled by the mechanism of liquid diffusion. The rate of drying, and therefore the diffusion coefficients, increased with the addition of sodium chloride and surface active agents. Diffusion coefficients were also influenced by air velocity and slice thickness, suggesting that the rate of drying of potato slices is controlled by a combination of internal and external resistances.     

Inactivation of Salmonella during drying and storage of apple slices treated with acidic or sodium metabisulfite solutions

This study was undertaken to determine whether pretreating inoculated Gala apple slices with metabisulfite or acidic solutions enhanced the inactivation of Salmonella during dehydration and storage. Apple slices inoculated with a five-strain mixture of Salmonella (7.6 log CFU/g) were pretreated, dried for 6 h at 60 degrees C, and stored aerobically at 25 degrees C for 28 days. Predrying treatments included (i) no treatment, (ii) 10 min of immersion in sterile water, (iii) 10 min of immersion in a 4.18% sodium metabisulfite solution, (iv) 10 min of immersion in a 3.40% ascorbic acid solution, and (v) 10 min of immersion in a 0.21% citric acid solution. Samples were plated on tryptic soy agar with 0.1% pyruvate (TSAP), brilliant green sulfa (BGS) agar, and xylose lysine tergitol 4 (XLT4) agar for the enumeration of bacteria. Populations were not significantly (P > 0.05) reduced by immersion in water but were reduced by 0.7 to 1.1 log CFU/g by immersion in acidic solutions. Immersion in the sodium metabisulfite solution reduced populations by 0.4, 1.3, and 5.4 log CFU/g on TSAP, BGS agar, and XLT4 agar, respectively. After 6 h of dehydration at 60 degrees C, populations on untreated and water-treated slices were reduced by 2.7 to 2.8, 2.7 to 2.9, and 4.0 to 4.2 log CFU/g as determined with TSAP, BGS agar, and XLT4 agar, respectively. In contrast, populations on slices treated with sodium metabisulfite, ascorbic acid, and citric acid were reduced after 6 h of dehydration by 4.3, 5.2, and 3.8 log CFU/g, respectively, as determined with TSAP; by 4.7, 5.5, and 3.9 log CFU/g, respectively, as determined with BGS agar; and by 5.5, 5.7, and 5.6 log CFU/g, respectively, as determined with XLT4 agar. Bacteria were still detectable by direct plating after 28 days except on slices treated with ascorbic acid. Immersion in metabisulfite or acidic solutions prior to dehydration should enhance the inactivation of Salmonella during the dehydration and storage of Gala apple slices.     

The effect of blanching on quality attributes of dehydrated carrots during long-term storage

 The effect of blanching on the retention of β-carotene and ascorbic acid, and non-enzymatic browning (NEB) during storage of dehydrated carrot slices was studied. Blanched carrots contained higher β-carotene but lower ascorbic acid than their unblanched counterpart just after drying, whereas NEB was unaffected by blanching. During storage of dehydrated carrots a decrease in β-carotene and ascorbic acid content with an increase in NEB values was observed. Blanching was helpful in limiting the loss of quality parameters irrespective of storage and packaging conditions. Received: 22 May 2000 / Revised version: 11 October 2000     

不同保鲜处理对鲜切苹果保鲜效果的影响

为研究不同保鲜剂及热处理对鲜切苹果品质的影响,将鲜切寒富苹果(Malus domestica)分别进行1.5%柠檬酸溶液、1.5%异抗坏血酸钠溶液、0.08%溶菌酶溶液和50℃的水浴中浸泡2 min处理,沥干后用0.08mm厚度CPP包装袋包装,抽真空(0.098 MPa)后密封,置于4℃冷库中贮藏,每3d测定与保鲜相关的生理生化指标。结果表明:不同保鲜剂及热处理均可适度延缓苹果切块感官品质、营养物质含量的下降、抑制微生物的繁殖,其中1.5%异抗坏血酸钠溶液浸泡处理的保鲜效果最好,能在4℃、15d贮藏期内较好地维持鲜切苹果的品质。     

Optimization of low-temperature blanching for retention of potato firmness: Effect of previous storage time on compression properties

Low-temperature blanching (LTB) of potatoes (cv. Kennebec), both without further processing and prior to cooking or freezing + cooking, significantly increased firmness retention as measured from compression parameters. The increase in firmness with respect to that of unblanched potatoes diminished in the order: blanched at 60 °C for 60 min and cooked > blanched at 60 °C for 60 min frozen and cooked > blanched at 60 °C for 60 min. Potato tubers were kept in refrigerated storage, and firmness, PME activity and dry matter (DM) content were periodically sampled over a period of 80 days. In the early stages of storage, PME activity lost 40% of its original value after 60 min at 60 °C, indicating that the contribution of starch breakdown products to the firmness of cooked and frozen cooked potatoes predominated over the effect of enzyme activity. With increasing time in storage, PME activity measured in the fresh tissue increased by 95% of its original value after 35 days; this resulted in changes in the pectic polymers which made for a firmer texture and different PME behaviour versus LTB temperature and time. A central composite rotatable design was used to study the effects of variation in levels of temperature (52.93–67.07 °C) and time (31.72–88.28 min) on compression parameters and PME activity. Stationary points showing maximum mechanical resistance had critical temperatures and times in the ranges of temperature (58–60 °C) and time (66–75 min) used for each independent variable. Results show a high correlation between PME activity and tissue firmness, suggesting that the contribution of the changes in the composition of the cell wall to the firmness of frozen cooked potatoes increased with increasing time in storage and reached a maximum in the intermediate stages of storage (35 days). Engineering stress (_u) proved to be the most appropriate compression parameter for detecting the firming effect that the PME activity produced on the frozen-cooked potato tissues as a consequence of LTB under these conditions.     

Ascorbic acid enhances destruction of Escherichia coli O157:H7 during home-type drying of apple slices

Destruction of Escherichia coli O157:H7 was evaluated on inoculated apple slices dehydrated at two temperatures with and without application of predrying treatments. Half-ring slices (0.6 cm thick) of peeled and cored Gala apples were inoculated by immersion for 30 min in a four-strain composite inoculum of E. coli O157:H7. The inoculated slices (8.7 to 9.4 log CFU/g) either received no predrying treatment (control), were soaked for 15 min in a 3.4% ascorbic acid solution, or were steam blanched for 3 min at 88 degrees C immediately prior to drying at 57.2 or 62.8 degrees C for up to 6 h. Samples were plated on tryptic soy (TSA) and sorbitol MacConkey (SMAC) agar media for direct enumeration of surviving bacterial populations. Steam blanching changed initial inoculation levels by +0.3 to -0.7 log CFU/g, while immersion in the ascorbic acid solution reduced the inoculation levels by 1.4 to 1.6 log CFU/g. Dehydration of control samples for 6 h reduced mean bacterial populations by 2.9 log CFU/g (TSA or SMAC) at 57.2 degrees C and by 3.3 (SMAC) and 3.5 (TSA) log CFU/g at 62.8 degrees C. Mean decreases from initial inoculum levels for steam-blanched slices after 6 h of drying were 2.1 (SMAC) and 2.0 (TSA) log CFU/g at 57.2 degrees C, and 3.6 (TSA or SMAC) log CFU/g at 62.8 degrees C. In contrast, initial bacterial populations on ascorbic acid-pretreated apple slices declined by 5.0 (SMAC) and 5.1 (TSA) log CFU/g after 3 h of dehydration at 57.2 degrees C, and by 7.3 (SMAC) and 6.9 (TSA) log CFU/g after 3 h at 62.8 degrees C. Reductions on slices treated with ascorbic acid were in the range of 8.0 to 8.3 log CFU/g after 6 h of drying, irrespective of drying temperature or agar medium used. The results of immersing apple slices in a 3.4% ascorbic acid solution for 15 min prior to drying indicate that a predrying treatment enhances the destruction of E. coli O157:H7 on home-dried apple products.     

不同热烫条件对胡萝卜榨汁效果的影响

通过试验了获得了热水、柠檬酸、醋酸、混合酸，蒸汽和微波6种热烫方式对胡萝卜榨汁效果影响的数据处理。其中榨汁效果主要从出汁率，可溶性固形物，pH值，颜色及稳定性5个方面进行评价。     

Kinetics of ascorbic acid degradation in potato blanching

Degradation kinetics of ascorbic acid was investigated on potato strips wrapped in aluminium foil and blanched for 5–60 min at different temperatures between 65°C and 100°C. The results confirmed that first order kinetics is adequate in describing the degradation reaction. The value of the rate constant was found to pass through a maximum at 85–90°C, indicating the inactivation of oxidative enzymes around these temperatures. Comparison of the degradation losses based on the present investigation with results on total loss of ascorbic acid during blanching reported in the literature showed that thermal degradation accounts for a considerable portion of the total loss.     

Reduction of acrylamide formation in potato slices during frying

Reduction of acrylamide formation in potato chips was investigated in relation to frying temperature and three treatments before frying. Potato slices (Tivoli variety, diameter: 37 mm, width: 2.2 mm) were fried at 150°C, 170°C and 190°C until reaching moisture contents of ∼1.7 g water/100 g (total basis). Prior to frying, potato slices were treated in one of the following ways: (i) soaked in distilled water for 0 min (control), 40 min and 90 min; (ii) blanched in hot water at six different time-temperature combinations (50°C for 30 and 70 min; 70°C for 8 and 40 min; 90°C for 2 and 9 min); (iii) immersed in citric acid solutions of different concentrations (10 and 20 g/l) for half an hour. Glucose and asparagine concentration was determined in potato slices before frying, whereas acrylamide content was determined in the resultant fried potato chips. Glucose content decreased in ∼32% in potato slices soaked 90 min in distilled water. Soaked slices showed on average a reduction of acrylamide formation of 27%, 38% and 20% at 150°C, 170°C and 190°C, respectively, when they were compared against the control. Blanching reduced on average 76% and 68% of the glucose and asparagine content compared to the control. Potato slices blanched at 50°C for 70 min surprisingly had a very low acrylamide content (28 μm/kg) even when they were fried at 190°C. Potato immersion in citric acid solutions of 10 and 20 g/l reduced acrylamide formation by almost 70% for slices fried at 150°C. For the three pre-treatments studied, acrylamide formation increased dramatically as the frying temperature increased from 150°C to 190°C.     

不同品种葡萄采后失水分析

鲜食葡萄采后果梗保绿和防止贮藏期间的干梗、果粒皱缩是衡量保鲜效果的重要指标.本文以我国近年来发展潜势较大的7个鲜食品种为试材,在室温条件下测定了果梗、果粒的失水情况,并对部分品种作了解剖学观察.研究结果表明:采后果梗失水由快到慢的顺序依次为:红地球、美人指、无核白鸡心、巨峰、意大利、秋黑、秋红葡萄;果粒失水由快到慢的顺序依次为:红地球、无核白鸡心、意大利、美人指、秋黑、秋红、巨峰葡萄.     

木瓜和玉米须提取物对双孢菇片烫漂后颜色和品质的影响

采用烫漂前浸泡和烫漂时添加两种方式,研究了木瓜和玉米须提取物对双孢菇片烫漂后颜色和品质的影响.结果表明,两种植物提取物的不同添加方式均可以在一定程度上抑制菇片的褐变和保持菇片的品质,其中浸泡前处理效果较好.木瓜和玉米须提取物最佳浸泡浓度均为2.0g/L,最大褐变抑制率分别为16.89％±4.57％、10.02％±3.24％;与清水浸泡前处理相比,维生素C含量分别提高67.30％、21.78％,总酚含量分别提高17.34％、38.29％.     

Influence of aqueous ozone, blanching and combined treatments on microbial load of red bell peppers, strawberries and watercress

The effectiveness of ozone in aqueous solution treatment on microbial inactivation was studied for three combinations microorganism/food: Listeria innocua/red bell peppers (artificially inoculated), total mesophiles/strawberries, and total coliforms/watercress, with two concentrations (0.3 and 2.0 ppm). Blanching treatments (50-60 °C) were also individually applied and in combination with ozone, for studying possible synergistic effects. In relation to ozone treatments, the highest microbial reductions were obtained for the highest concentration with the highest treatment time (3 min). Under those conditions, L. innocua/peppers, total mesophiles/strawberries and total coliforms/watercress were reduced respectively 2.8 ± 0.5, 2.3 ± 0.4 and 1.7 ± 0.4 log-cycles. However, a substantial portion of the microbial populations were reduced by water washing alone, and the presence of ozone generally added an additional reduction of 0.5-1.0 log-cycles.If ozone at the highest concentration is used, the treatment impacts on L. innocua/peppers and total mesophiles/strawberries load reductions were equivalent to a blanching at 50 °C (for the same treatment times).Combining blanching and ozone did not generate synergistic effects, and in some situations microbial reductions were lower than the ones observed when treatments were applied independently.