牦牛血低聚肽抗缺氧活性及其作用机制

研究牦牛血低聚肽的抗缺氧活性及其可能的作用机制。将75 只小鼠随机分为缺氧对照组、阳性对照组（红景天组）和牦牛血低聚肽高、中、低剂量组,连续灌胃给药30 d,末次灌胃60 min后,置于常压密闭容量瓶中直到呼吸停止,即刻取出心肌组织和脑组织并测定相关指标。结果表明：牦牛血低聚肽具有良好的抗缺氧活性,能够保护常压密闭缺氧小鼠的大脑及心脏。与对照组相比,高剂量牦牛血低聚肽干预能够极显著降低常压缺氧小鼠脑和心肌组织中丙二醛含量、H2O2含量和乳酸脱氢酶活力（P＜0.01）,显著提高常压缺氧小鼠脑和心肌组织中超氧化物歧化酶活力（P＜0.01）、谷胱甘肽过氧化物酶活力（心肌组织中P＜0.01,脑组织中P＜0.05）、过氧化氢酶活力（P＜0.01）,极显著提高常压缺氧小鼠脑和心肌组织中三磷酸腺苷含量（P＜0.01）。以上结果表明：牦牛血低聚肽能够缓解缺氧对机体导致的氧化应激损伤,作用机制可能与清除自由基、抑制脂质过氧化、维持机体抗氧化酶系活性及维持线粒体内氧化磷酸化的正常进行有关。     

厚朴抗氧化作用研究

分别用ＤＰＰＨ法,硫代巴比妥酸法及Ｎａ２Ｓ２Ｏ３滴定法测定了厚朴提取物的抗氧作用。结果表明：不同溶剂厚朴提取物对ＤＰＰＨ自由基均有清除作用,其中以乙醇提取物清除能力最强。厚朴乙醇提取物对亚油酸、猪油的脂质过氧化有良好的阻断作用,其抗氧化作用随提取物用量的增加而逐渐增强。对猪油的抗氧化作用以６０％乙醇提取的活性最强     

月季花抗氧化作用的研究

分别用 DPPH法、硫代巴比妥酸法、Na2 S2 O3- I2 滴定法测定了月季花提取物的抗氧作用。结果表明 ,不同溶剂的月季花提取物对 DPPH自由基均有一定的清除作用 ,其清除率大小依次为 95 %乙醇、水、6 0 %乙醇、乙酸乙酯提取物。随着月季花提取物浓度的增加 ,对猪油和亚油酸的氧化抑制作用增强 ,其中以 95 %乙醇提取物对猪油的抗氧化作用最强     

油茶肉质果、肉质叶提取液的保健功效

研究油茶肉质果、肉质叶提取液（EFF，EFY）的抗自由基、降血糖和抗癌作用。采用TBA比色法和小鼠红细胞氧化溶血抑制试验测定抗自由基作用；采用四氧嘧啶诱导糖尿病小鼠模型，分别用EFF和EFY灌胃7d测定降血糖作用；利用H22移植性实体瘤的方法研究抗癌作用。EFF具有显著消除体内自由基及抗脂质过氧化作用，与维生素E抗自由基及脂质过氧化效果相同；对实验性糖尿病小鼠血糖有明显的降低作用，接近中药消渴丸的效果；能明显抑制小鼠H22移植性实体瘤的生长，油茶肉质果组的抑瘤率为54．9％，油茶肉质叶组的抑瘤率为30．2％，对动物白细胞、脾指数、胸腺指数无明显影响。EFF和EFY能有效控制糖尿病小鼠的血糖水平，维持正常代谢，对正常血糖小鼠无影响；具有提高SOD活性，清除自由基的作用；对癌症小鼠有显著的抑癌效果，与已报道的20种具有显著抗癌作用的蔬菜相比，油茶肉质果排在第9位，油茶肉质叶排在第15位。     

山稔子黄酮类提取物抗自由基作用及体内抗氧化功能的研究

本实验研究山稔子黄酮类提取物的抗自由基作用及体内抗氧化功能。通过测定小鼠血清SOD、GSH—Px活性和MDA含量的变化,研究山稔子黄酮类提取物的体内抗氧化功能;并通过测定山稔子黄酮类提取物清除羟自由基率和抑制超氧自由基率的实验,证明山稔子黄酮类提取物的体外抗自由基作用。结果表明,山稔子黄酮类提取物可显著增强小鼠血清SOD、GSH。Px活性,降低MDA含量,并可有效地清除羟自由基和超氧自由基。说明山稔子黄酮类提取物具有明显的抗自由基作用及体内抗氧化功能。     

共轭亚油酸抗缺氧效果及其机制的探讨

为了探讨共轭亚油酸的抗缺氧效果，分别以浓度为1．5％，0．5％，0．1％和0％（对照）的共轭亚油酸给小鼠灌胃，连续10d，在常压缺氧条件下测定小鼠存活时间，并测定小鼠心肌和骨骼肌肌红蛋白含量，结果显示，在浓度为0．5％时，CLA能显著提高小鼠在缺氧条件下的生存时间，并显著提高小鼠心肌和骨骼肌肌红蛋白含量。     

丹参提取物耐缺氧功能及营养化学研究

目的：探讨丹参提取物对小鼠耐常压缺氧功能及其功能营养化学基础。方法：用常压缺氧小鼠模型试验研究提取物耐缺氧活性，对提取物中功能成分和营养素进行测定和比较。结果：丹参提取物能显著延长小鼠耐常压缺氧的存活时间。提取物中含有丰富的耐缺氧功能因了和相关性营养成分，其中，醇提物中丹参酮IIA1.43％、隐丹参酮0．51％、丹参酮10．1％、丹参素2．14％、原儿茶醛0．33％、多糖含量4．6％。每100g提取物中VC、VE和胡萝卜素的含量分别为0．722mg、25．9mg和0．47mg。结论：丹参提取物具有增强模型小鼠耐常压缺氧能力，其中耐缺氧功能因了和相关性营养素含量丰富。提示这些成分是其耐缺氧作用的物质基础。丹参提取物作为功能食品基料具有良好的应用基础。     

红景天超临界CO_2提取物抗氧化作用研究

对红景天超临界CO_2提取物抗氧化作用进行研究。采用二苯代苦味酰基自由基分析法、邻苯三酚自氧化法、过氧化氢检测法和过氧化值测定法,测定了红景天超临界CO_2提取物对二苯代苦味酰基自由基、超氧阴离子和羟基自由基的清除能力以及对油脂过氧化作用的影响。结果表明:红景天超临界CO_2提取物具有明显清除自由基的能力,对猪油,菜油有较强的抗氧化作用。     

决明子茶提取物中黄酮含量的测定及抗自由基活性研究

以水、不同含量乙醇为溶剂,采用超声、回流等方法提取决明子茶中的总黄酮,采用亚硝酸钠-氯化铝法,通过紫外分光光度计在494 nm处测定样品吸收,计算总黄酮含量;采用DPPH法研究提取物抗自由基活性。研究发现超声和回流提取均含有一定量的黄酮,采用水回流提取的含量最低为3.39%,55%醇超声提取的含量最高为6.10%。决明子醇提取物对DPPH自由基有较好的清除作用,55%醇回流提取物自由基清除率为71.90%。     

蕨麻提取物的抑菌作用及其稳定性研究

利用琼脂打孔扩散法研究蕨麻水提取物、60% 乙醇提取物和乙酸乙酯提取物对6 种常见食源性污染菌的抑制作用;并探讨pH 值和热处理对蕨麻60% 乙醇提取物抗菌活性的影响。结果表明：3 种提取物对6 种食源性污染菌均有抑制作用,其中60%乙醇提取物抑菌效果最好;在pH 值为7 时60%乙醇提取物对6 种食源性污染菌的抑制效果均达到最大;加热对蕨麻60%乙醇提取物抑菌效果的影响与温度和时间均有关,在121℃加热30min 时,抑菌活性有明显下降。     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the     

Chinese CTP Market

According to Printing and Printing Equipment Industries Association of China（PEIAC）＇s statistics to the plate manufucturer in China, in 2013, the actual offset plate production has reached 346 million square meters in China. Among them, the CTP production volume was 245 million square meters, up by 11% than that of last year; the total sales of the CTP plate was 239 million square meters, up by 13%.     

Preparatory Work of Print China 2015 is Going Smoothly

正Held at Guangdong Modern International Exhibition Center,Print China 2015 will cover 7exhibition halls,besides the original Hall No.3,4,5,6,7,the newly built F zone of Hall 3 will be used too.The total area will be140,000 square meters.Hall 3:Offset and large printing equipment,package printing equipment,post press