共查询到19条相似文献,搜索用时 63 毫秒
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采用反相高效液相色谱法-紫外检测器(RP-HPLC-UV)测定雷公藤药材中雷公藤甲素的含量,色谱柱为Kromasil C18柱(150 mm×4.6 mm,5μm),甲醇∶水溶液(45∶55)为流动相进行等度洗脱,流速1.0 mg/min,进样量20μL,紫外检测波长λ=218 nm。结果表明,每个样本分析时间约10 min,雷公藤甲素保留时间约6.103 min,雷公藤甲素与其他成分分离效果良好,标准曲线y=0.206 7x+2.123 9,r=0.993 3(n=5),线性范围0.01~0.1 mg/m L(r=0.993 3),回收率为96.00%~99.03%,RSD≤2%。本方法专属性强,准确可靠,可以用于雷公藤药材中雷公藤甲素的含量测定。 相似文献
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HPLC法测定雷公藤药材中雷公藤甲素的含量 总被引:1,自引:0,他引:1
建立了雷公藤药材中雷公藤甲素的含量测定方法。采用Gemini-C18(250 mm×4.60 mm,5μm)色谱柱;流动相:乙腈-水(25∶75);检测波长218 nm;流速1.0 mL.min-1,柱温为30℃。结果表明:雷公藤甲素在5.82~58.20μg.mL-1浓度范围内与峰面积呈良好的线性关系(r=0.9996),平均回收率为101.49%,RSD=2.02%。本方法简便、准确、重现性好,可用于雷公藤药材中雷公藤甲素的含量测定。 相似文献
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目的:建立HPLC测定海棠合剂中雷公藤甲素含量测定方法。方法:采用HPLC法,色谱柱为Diamonsil C18柱(250mm×4.6mm,5μm)柱;以乙腈(A)-水(B)为流动相,梯度洗脱(0~20分钟,20%A→50%A;20~21分钟,50%A→20%A),流速为1.0m L/min,柱温35℃,UV检测波长为220nm。结果:雷公藤甲素在进样量在0.02096~0.2096μg范围内与峰面积呈很好的线性关系,回归方程和相关系数:回归方程为A=1586.937C+2.495342,r=0.9999;平均回收率为93.5%,RSD=2.95%(n=6)。结论:该方法灵敏快速,操作简便,重现性好,可用于海棠合剂的质量控制。 相似文献
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为降低雷公藤甲素的毒副作用,制备雷公藤甲素脂质体粉雾剂(TP-LD),并进行初步评价。方法采用乙醇注入法和冷冻干燥法制备雷公藤甲素脂质体粉雾剂,并对其进行处方工艺的研究和制剂学评价。结果 雷公藤甲素脂质体包封率为(74.6±1.7)%,粒径大小为(113.03±2.4) nm; Zeta电位为(-43.60±1.2) mv;冻干复溶后TP-LD的粒径大小为(1.17±0.047)μm,流动性与亲水性均较好,满足粉雾剂给药的基本要求。结论 成功制备雷公藤甲素脂质体粉雾剂,工艺简单可行、质量稳定。 相似文献
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对市售不同产地的雷公藤饮片中雷公藤甲素和雷公藤红素进行含量测定。采用超高液相色谱法。色谱柱为Waters Acquity UPLC BEH C_(18)柱(2.1 mm×100 nm,1.7μm),流动相为乙腈-0.1%磷酸水,梯度洗脱,流速为0.5 mL/min,柱温25℃,检测波长210 nm;两种有效成分在各自的线性范围内线性关系良好,该实验所建立的方法简便,重复性好。不同产地雷公藤饮片中雷公藤甲素和雷公藤红素的含量有显著差异,表明市售雷公藤饮片的质量存在明显差别,为雷公藤饮片进一步的临床应用提供参考。 相似文献
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制备包裹白藜芦醇的柔性纳米脂质体,并优化配方,得到一个稳定性好、负载量高的体系。实验采用注入法制备柔性纳米脂质体;制备出的柔性纳米脂质体采用光子相关光谱(PCS)和激光衍射(LD)测得粒径为250nm~300nm,PDI为0.1~0.2;透射电镜观察出的脂质体形貌呈球形或椭球形;用高效液相色谱测出白藜芦醇的含量为0.7%~1.1%,采用超滤离心法测得的包封率为99.7%。体系于4℃、25℃以及40℃下分别保存2个月,粒径无明显变化。采用注入法制备柔性纳米脂质体,操作简便,制备出的脂质体性状稳定。 相似文献
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分别制备雷公藤甲素普通脂质体、β-环糊精(β-CD)包合物脂质体和羟丙基-β-环糊精(HP-β-CD)包合物脂质体,采用透析法进行体外释药实验并绘制释药曲线,对比分析3种脂质体在不同释放介质中的释药特点。研究发现,不同释放介质会对3种雷公藤甲素脂质体的体外释药曲线具有不同程度的影响,影响最大的是β-CD包合物脂质体、其次是普通脂质体,影响最小的是HP-β-CD包合物脂质体。在与人体体液更为接近的磷酸盐缓冲溶液(PBS)中,与普通脂质体相比,β-CD包合物脂质体具有明显的持续缓释作用,其累积释药率为60.0%;而HP-β-CD包合物脂质体释药初期具有缓释作用,中期释药加速,后期又趋缓,其累积释药率为90.0%,释药最为充分。 相似文献
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以β-甘油磷酸钠(GP)为羟丙基壳聚糖(HPCS)或壳聚糖季铵盐(HTCC)的温敏交联剂、氯化钙(CaCl_2)为海藻酸钠(SA)的螯合交联剂,采用共混工艺,制备以壳聚糖衍生物-GP为第1网络、海藻酸钙为第2网络的可注射温敏互穿水凝胶。利用高斯软件基于氢键理论探讨凝胶温敏相变机理。利用红外光谱仪、扫描电子显微镜、热重分析仪和流变分析仪对凝胶性能进行了表征。当ρ_(HPCS)=18.75 mg/mL,ρ_(GP)=75 mg/mL,ρ_(SA)=18.33 mg/mL和ρ(CaCl_2)=1.67 mg/mL时,凝胶在37℃温敏凝胶化时间为(85±15)s,压缩弹性模量为(54±15)kPa,损耗模量G″和储能模量G′比值为0.10±0.025。由此可知,HPCS-GP/Ca-Alg复合凝胶可在生理温度环境凝胶化且时间可控,凝胶机械强度良好,在组织工程领域中具有应用潜力。 相似文献
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Thermosensitive copolymers of poly(N‐isopropyl acrylamide)‐co‐2‐hydroxyethyl methacrylate (NIPA‐co‐HEMA) macroporous resins were synthesized in the form of beads by inverse suspension polymerization. The copolymerization was carried out in aqueous solutions of the comonomers dispersed by cyclohexane with stabilizers. A series of resins with different molar ratios of NIPA : HEMA, and different crosslinking degrees was obtained. The compositions of the copolymers were determined by elemental analysis. The results showed that the content of HEMA in a copolymer was higher than that of the corresponding feed mixture from which the copolymer was made. IR spectra also confirmed the structure of the copolymers. The porous parameters such as true densities, apparent densities, pore volumes, and porosities of the resins were measured by means of pycnometry. The determination of equilibrium swelling ratios and measurement of differential scanning calibration indicated that the resins exhibited thermosensitivity in aqueous solutions. Finally, the loading of hydroxyl groups was determined by titration. The resins have potential applications as supports in solid‐phase synthesis after being functionalized. © 2003 Wiley Periodicals, Inc. J Appl Polym Sci 91: 1792–1797, 2004 相似文献
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采用原位一步法合成了温敏改性聚 N-异丙基丙烯酰胺(PNIPAAm)/SiO2纳米复合物.
通过接触角对温敏改性的纳米SiO2进行了条件优化:反应温度73℃,反应时间6 h,单体用量与KH-570摩尔比为1:1,引发剂用量为单体用量与KH-570总质量的2.5%。通过TEM、FT-IR、XPS等手段对温敏性纳米SiO2的组成和结构进行了表征。同时,这一有机-无机纳米复合物仍然保持PNIPAAm 的温度响应性, 最低临界溶解温度(LCST)与纯PNIPAAm相似。 相似文献
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Bis[4-(phenyl-isopropyl)phenyl]-4-nitrophenylamine (BiPNPA) (iii) was firstly synthesized via nucleophilic substitution of 4-fluoronitrobenzene (ii) and bis-[4-(phenyl-isopropyl)phenyl]amine (BiPA) (i) in this study, and then compound (iii) was reduced to bis[4-(phenyl-isopropyl)phenyl]-4-aminophenylamine (BiPAmPA) (iv). At last, acrylation of BiPAmPA with acryloyl chloride was performed to obtain a novel bis[4-(phenyl-isopropyl)phenyl]-4-acrylamidophenylamine (BiPAcPA) compound (v). Then, a series of thermosensitive hydrogels were prepared by copolymerization of BiPAcPA and N-isopropyl acrylamide (NIPAAm) by UV irradiation. The effect of BiPAcPA content in the copolymeric hydrogels on the swelling ratio, mechanical properties, and drug release behaviors of the hydrogels was investigated. Results showed that the swelling ratios of the copolymeric hydrogels decreased from 8.2 (g/g) to 5.01 (g/g) when the monomer BiPAcPA content in the copolymeric hydrogel increased from 0.1 mol% to 0.9 mol%. In addition, the thermosensitive behavior obviously decreased with increasing of BiPAcPA content in the hydrogels, but the mechanical properties and crosslinking density of the hydrogel increased with increasing of BiPAcPA content; the caffeine release ratio from 46 % decreased to 29 %. The result of caffeine release also showed that the caffeine-loading amounts decreased with increasing content of BiPAcPA in the hydrogel, but their fractional release increased. In addition, the corresponding copolymers of the copolymeric hydrogels were prepared. Furthermore, the UV absorbance and photoluminescent (PL) behavior of the BiPAcPA, NIPAAm/BiPAcPA copolymeric hydrogels, and their corresponding copolymers in some solvents with different polarities were investigated. The results showed the PL intensity increased with increasing BiPAcPA amounts, especially the wavelength had obvious blue shift phenomenon in THF solvent. The wavelength of UV absorbance and PL emission of BiPAcPA all appeared in visible region. No matter what BiPAcPA monomer, NIPAAm/BiPAcPA hydrogels or NIPAAm/BiPAcPA copolymers, the wavelengths of UV absorbance and PL emission in DMSO solvent are all the same. 相似文献
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The thermosensitive material that could be transformed into gel at 37°C was prepared from chitosan (dissolved in acetic acid/sodium acetate buffer solution) and a mixture of α‐ and β‐glycerophosphate (αβ‐GP). The thermosensitive characteristics, appearance, and structure of the hydrogel were all affected by the pH, ionic strength, and CS/αβ‐GP ratio. The optimal conditions for the preparation of a transparent CS‐αβ‐GP thermosensitive hydrogel were pH 4.6, ionic strength 0.15 mol/L, and a CS/αβ‐GP ratio of 8.8/1.2 (v/v). The hydrogel was stable for at least 3 months at 4°C. We believe that hydrogen bonding interactions between the N? H (and C?O) groups of chitosan and the O? H groups of αβ‐GP play an important role during the process of sol‐to‐gel transition. The cumulative release of adriamycin from the CS‐αβ‐GP hydrogel, measured in PBS at pH 7.4, reached only 60 to 70% over 24 h, indicating that this material could be potentially used in a sustained drug delivery system. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2009 相似文献
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采用化学共沉淀结合水热处理的方法,控制一定的反应条件,制备了碳纳米管/四氧化三铁(CNTs/Fe3O4)热种子材料,然后以壳聚糖温敏凝胶为载体,将CNTs/Fe3O4复合材料分散到凝胶中,制备出微波靶向热疗用碳纳米管-四氧化三铁-壳聚糖温敏凝胶。碳纳米管复合热种子材料在凝胶体系中分布均匀,与载体不发生化学反应,25 W下辐照10 min,当CNTs/Fe3O4复合材料的质量浓度为20 mg/mL时,体系温度在初始温度基础上升高了38.7℃,在微波场中具有良好的产热能力,可以满足微波热疗的要求。 相似文献
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Soo Nam Park Hye Jin Lee Hae Soo Kim Min A. Park Hyun A. Gu 《Korean Journal of Chemical Engineering》2013,30(3):688-692
We sought to evaluate the transdermal permeation potential of quercetin-loaded ethosomes. Quercetinloaded ethosomes were prepared and characterized with regard to particle size, loading efficiency, stability, and in vitro skin permeation. The optimized formulation of ethosomes was confirmed using 2% egg phosphatidylcholine and hydrated 20% ethanol. After quercetin was applied using this formulation, the stability of the ethosomes was determined when loaded with up to 0.04% quercetin. We observed that loading efficiency was improved with increasing concentrations of quercetin. Ethosomes loaded with 0.04% quercetin showed both the greatest loading efficiency (63.9%±6.0%) and an optimal size range (132±32 nm). Ethosomes loaded with quercetin were superior in skin permeation ability (29.5±7.0 μg/cm2) compared to either ethanolic solution or liposomes. Therefore, we concluded that quercetin-loaded ethosomes increased the skin delivery of quercetin. Our results suggest that quercetin-loaded ethosomes may enhance the effect of cosmetic materials. 相似文献