抗菌肽Chionodracine对痤疮丙酸杆菌抑菌及抗炎作用的研究

痤疮丙酸杆菌了参与寻常痤疮的发病和炎症过程.本文以冰鱼抗菌肽Chionodracine为研究对象,在体外和体内对其抗痤疮丙酸杆菌和抗炎作用进行了研究.Chionodracine对痤疮丙酸杆菌的最小抑菌浓度为12.5 μM;对热和pH有一定的抗性;对正常人表皮角质细胞无明显的毒性;对痤疮丙酸杆菌造成的大鼠耳廓肿胀具有较好...     

广藿香精油抗炎祛痘功效研究

采用气相色谱-质谱联用技术对广藿香精油成分进行分析。通过细胞毒性实验确定广藿香精油对人急性单核细胞(THP-1)的安全质量浓度。利用痤疮丙酸杆菌(P.acnes)刺激THP-1建立的特异性痤疮炎症细胞模型考察广藿香精油对炎症因子IL-1β的影响。通过荧光实时定量逆转录聚合酶链反应(qRT-PCR)初步探讨广藿香精油抗炎祛痘的作用机制。研究结果表明:广藿香精油在最大安全质量浓度12.5 mg/L时,对炎症细胞模型中P.acnes活性影响较小,对IL-1β的分泌具有明显的抑制作用(抑制率66.54%),可显著降低细胞内Toll样受体2(TLR2)mRNA的转录(抑制率73.73%),从而起到抑制炎症信号转导和继发性炎症介质生成的作用。     

中草药祛痘复方对痤疮致病菌和外泌酶的影响

通过药敏试验和抑菌试验考察中草药祛痘复方对痤疮丙酸杆菌(Propionibacterium acnes,PA)、金黄色葡萄球菌(Staphylococcus aureus,SA)和表皮葡萄球菌(Staphylococcus epidermidis,SE)的抑菌功效,同时考察其对3种菌的抑菌时间动力学的影响。采用脂肪酶和蛋白酶水解底物形成的透明圈,研究中草药祛痘复方对PA释放脂肪酶和蛋白酶的影响。结果表明,中草药祛痘复方明显抑制了PA、SA和SE,并呈时间和剂量依赖性关系;中草药祛痘复方抑制脂肪酶和蛋白酶的作用主要是通过抑制PA而发挥作用。     

大麻二酚的抗炎和抑菌活性研究

采用CCK-8法考察大麻二酚(CBD)对HaCat细胞和RAW264.7细胞相对细胞活性的影响.采用LPS诱导的HaCat细胞和RAW264.7细胞炎症模型考察CBD对炎症因子分泌的抑制作用.采用抑菌环和抑菌率等实验方法对CBD抑制厌氧菌痤疮丙酸杆菌(P.acnes)的效果进行测试.结果表明,CBD浓度低于20?μmo...     

痤疮丙酸杆菌对IPEC-J2细胞生长及细胞因子转录时相影响的研究

目的探讨痤疮丙酸杆菌对猪空肠上皮细胞IPEC-J2生长及细胞因子转录时相的影响。方法通过显微镜观察细胞形态变化;台盼蓝拒染检测细胞存活率;实时荧光定量PCR检测IL-8、IL-10、IL-12和ZO-1基因表达量的变化。结果高浓度痤疮丙酸杆菌与IPEC-J2细胞共培养后细胞形态发生改变,活细胞数量明显减少;荧光定量PCR检测表明,痤疮丙酸杆菌能明显上调IL-8和IL-12基因的表达,明显下调ZO-1基因的表达。IL-10基因在IPEC-J2细胞中不表达。结论痤疮丙酸杆菌在体外可引起IPEC-J2细胞炎性分子分泌增加,紧密连接功能的下降。     

祛痘功效成分的技术研究与产品应用的开发展望

<正>祛痘是当前护肤品市场上比较热门的化妆品功效宣称之一。祛痘化妆品对于患有痤疮问题的消费者而言,可作为日常护理产品,能起到一定的辅助改善作用而受到消费者的欢迎。本文通过对祛痘的作用机理和市场趋势的分析,对现有常用的祛痘功效成分进行筛选与说明,并对未来祛痘化妆品的开发提出展望。     

胸膜肺炎放线杆菌血清型特异的疫苗候选基因的筛选及痤疮丙酸杆菌异源免疫

目的筛选猪胸膜肺炎放线杆菌(APP)1型与5型特异的疫苗候选基因,并研究痤疮丙酸杆菌对猪传染性胸膜肺炎的异源免疫。方法采用cDNA代表性差异分析、大肠杆菌核糖体展示、免疫筛选和RT-PCR技术筛选APP1型和5型血清型特异的疫苗候选基因,进行克隆、测序及同源序列分析。并用与APP疫苗候选基因具有同源序列的痤疮丙酸杆菌(PA)免疫小鼠,研究其对APP感染的异源免疫。结果获得血清1型APP6条疫苗候选基因,2条为未知基因,2条与PA同源性为98%,2条与人cDNA有同源性。获得血清5型APP7条疫苗候选基因,其中2条为未知基因,2条与PA同源性分别为93%和100%。用PA全菌免疫小鼠,产生的抗血清可与1型和5型APP发生强的免疫反应,ELISA检测效价达1∶3200;脾淋巴细胞检测结果显示,免疫组CD3+、CD4+T细胞的百分率及CD4+/CD8+比值与对照组相比均升高,两组CD3+T细胞百分率差异有显著意义。以10倍LD501型和5型APP分别感染PA免疫小鼠,小鼠存活率分别为95%和90%,并且在攻毒后第15天体内APP可全部被清除。结论1型与5型APP存在不同的疫苗候选基因,痤疮丙酸杆菌与之存在共同抗原,能够产生交叉免疫应答,对血清1型和5型APP的感染具有预防作用。     

不同比例荆芥-连翘药对挥发油成分及抗痤疮致病菌研究

采用GC-MS测定不同质量比(1:1、1:2.5、2.5:1)荆芥-连翘药对及其单味饮片挥发油的化学成分,结合抗痤疮丙酸杆菌、金黄色葡萄球菌和表皮葡萄球菌的作用,以探究荆芥-连翘药对抗痤疮致病菌的配伍意义.结果表明,荆芥挥发油主要含有胡薄荷酮(41.52％)和异薄荷酮(41.62％),连翘挥发油主要含有β-蒎烯(38....     

橙皮苷分析方法研究

以橙皮苷为标准品,采用紫外分光光度法(直接法)和高效液相色谱(HPLC)测定橙皮苷含量,并对测定条件进行探讨,比较两种方法测定橙皮苷的准确度。实验结果表明,紫外分光光度法测得的橙皮苷纯度数据比高效液相色谱数据高,HPLC法精确,分光光度法简单快速,均能得到令人满意的结果。     

从柑桔皮中联产果胶和橙皮苷的新工艺

介绍了连续法从柑桔皮中提取果胶和橙皮苷的新工艺。该工艺所得果胶产品各项指标达到了国家标准，橙皮苷产品达到或超过了国外文献标准。     

Therapeutic Effect of a Newly Isolated Lytic Bacteriophage against Multi-Drug-Resistant Cutibacterium acnes Infection in Mice

Acne vulgaris, which is mostly associated with the colonization of Cutibacterium acnes (C. acnes), is a common skin inflammatory disease in teenagers. However, over the past few years, the disease has extended beyond childhood to chronically infect approximately 40% of adults. While antibiotics have been used for several decades to treat acne lesions, antibiotic resistance is a growing crisis; thus, finding a new therapeutic target is urgently needed. Studies have shown that phage therapy may be one alternative for treating multi-drug-resistant bacterial infections. In the present study, we successfully isolated a C. acnes phage named TCUCAP1 from the skin of healthy volunteers. Morphological analysis revealed that TCUCAP1 belongs to the family Siphoviridae with an icosahedral head and a non-contractile tail. Genome analysis found that TCUCAP1 is composed of 29,547 bp with a G+C content of 53.83% and 56 predicted open reading frames (ORFs). The ORFs were associated with phage structure, packing, host lysis, DNA metabolism, and additional functions. Phage treatments applied to mice with multi-drug-resistant (MDR) C.-acnes-induced skin inflammation resulted in a significant decrease in inflammatory lesions. In addition, our attempt to formulate the phage into hydroxyethyl cellulose (HEC) cream may provide new antibacterial preparations for human infections. Our results demonstrate that TCUCAP1 displays several features that make it an ideal candidate for the control of C. acnes infections.     

家蝇抗菌肽祛痘功效性评价及其产品开发

采用肉汤稀释法测得家蝇抗菌肽对痤疮丙酸杆菌(Propionibacterium acnes)的最低抑菌浓度(MIC)为0.078 mg/mL.根据GB 15979-2002《一次性使用卫生用品卫生标准》中附录C4“溶出性抗(抑)菌产品抑菌性能试验方法”,测得家蝇抗菌肽在添加质量分数为1％,2％,3％和5％时的抑菌率分别为34.21％,42.42％,99.99％和99.99％.根据QB/T1857-2004中润肤膏霜的卫生指标及感官、理化指标的试验方法及检验标准对该祛痘产品进行检测,结果表明各项指标均符合标准中的要求,且进一步的加速稳定性试验表明,产品的稳定性较好,未见分层、变色、变质和变味等现象的发生.     

橙皮甙提取纯化工艺的优化

研究以Ca(OH)2溶液做溶剂从橘皮中提取橙皮甙的工艺条件。得以桔皮为原料,,用10倍量7 g/L Ca(OH)2,提取2h,提取液调pH=5,得率2.9%以上,重结晶得精制橙皮甙,纯度可达98%,收率为75%。此方法操作简便,易控制且提取橙皮苷的效率较高。     

Micro-Current Stimulation Suppresses Inflammatory Responses in Peptidoglycan-Treated Raw 264.7 Macrophages and Propionibacterium acnes-Induced Skin Inflammation via TLR2/NF-κB Signaling Pathway

Acne is a common inflammatory disorder of the human skin and a multifactorial disease caused by the sebaceous gland and Propionibacterium acnes (P. acnes). This study aimed to evaluate the anti-inflammatory effect of micro-current stimulation (MC) on peptidoglycan (PGN)-treated raw 264.7 macrophages and P. acnes-induced skin inflammation. To specify the intensity with anti-inflammatory effects, nitric oxide (NO) production was compared according to various levels of MC. As the lowest NO production was shown at an intensity of 50 μA, subsequent experiments used this intensity. The changes of expression of the proteins related to TLR2/NF-κB signaling were examined by immunoblotting. Also, immunofluorescence analysis was performed for observing NF-κB p65 localization. All of the expression levels of proteins regarding TLR2/NF-κB signaling were decreased by the application of MC. Moreover, the application of MC to PGN-treated raw 264.7 cells showed a significant decrease in the amount of nuclear p65-protein. In the case of animal models with P. acnes-induced skin inflammation, various pro-inflammatory cytokines and mediators significantly decreased in MC-applied mice. In particular, the concentration of IL-1β in serum decreased, and the area of acne lesions, decreased from the histological analysis. We suggest for the first time that MC can be a novel treatment for acne.     

无患子水提液的微生物发酵纯化及无患子乳酸杆菌发酵液抗痤疮功效

无患子（Sapindus mukorossi Gaertn）提取液常作为一种天然活性原料添加到洗护产品中,为推进无患子提取液在日化行业的应用,采用微生物发酵法对无患子水提液纯化,以耗糖量和皂苷纯度为指标,筛选出植物乳酸杆菌（Lactobacillus plantarum）作为发酵菌株,制得了无患子乳酸杆菌发酵液（SF）。从皮脂、炎症和致病菌的过度繁殖3个方面,研究了SF的抗痤疮功效。结果显示,质量浓度为20 μg/mL的SF对SZ95细胞脂质合成率降低了26.4%;对脂多糖（LPS）诱导的RAW 264.7细胞的一氧化氮（NO）、肿瘤坏死因子α（TNF-α）、白细胞介素-6（IL-6）和活性氧（ROS） 分别降低了52.3%、57.5%、56.2%和35.9% ;采用二倍稀释法测定了SF对痤疮丙酸杆菌、糠秕马拉色菌和金黄色葡萄球菌的最小抑菌质量浓度（MIC）别为3.125、12.5和6.25 g/L;人体功效评价结果显示,受试者面部痤疮情况显著改善。     

UPLC-MS/MS 法检测祛痘类化妆品中盐酸特比萘芬含量

采用超高效液相色谱-串联质谱法(UPLC-MS/MS)建立了祛痘类化妆品中盐酸特比萘芬的检测方法。待测样品以甲醇为提取溶剂经超声提取,上清液经微孔滤膜过滤后测定。以10 mmol/L乙酸铵水溶液-乙腈为流动相,BEH-C18色谱柱分离,采用多反应离子监测(MRM)模式进行定性定量分析。所建方法中,待测组分检出限为0.02 mg/kg,定量限为0.05 mg/kg,在低、中、高3个添加水平范围内平均回收率为89.0%~117.7%,相对标准偏差为1.1%~4.7%。用于80批祛痘类化妆品样品检测,其中1批检出盐酸特比萘芬。