Ti-Zr-Cu合金的抗菌性能和体外生物相容性

采用平板共培养法测定Ti-Zr-Cu合金表面的菌落数,通过CCK8细胞增殖检测、鬼笔环肽细胞染色观察细胞核和细胞骨架形貌、细胞凋亡和黏附能力测定,研究了Ti-Zr-Cu合金的抗菌性能和体外生物相容性。结果表明,空白样品和Ti-Zr合金表面上的菌落密集,而Ti-Zr-Cu合金表面上的菌落数极少。Ti-Zr-Cu合金对金黄色葡萄球菌和大肠杆菌的抗菌率分别达到98.28%和97.67%,表现出优异的抗菌性能。MC3T3-E1在Ti-Zr-Cu合金表面培养1、4、7 d的相对增值率分别为168.8%、109.8%和106.5%,均高于100%,表明这种合金没有细胞毒性。细胞在Ti-Zr-Cu合金表面上的附着和铺展良好,有利于贴壁细胞在其表面黏附和进一步增值,表明Ti-Zr-Cu合金具有良好的生物相容性。Ti-Zr-Cu合金对细胞凋亡没有不良影响。从Ti-Zr-Cu合金表面细胞的SEM照片可见细胞的粘附正常,也表明其具有良好的生物相容性。     

不同铜含量钴铬合金的体外生物相容性

用CCK-8法和Annexin-V/PI双标记法研究了不同铜含量(质量分数,下同)的钴铬合金和钴铬合金的体外生物相容性(包括L929细胞增殖和凋亡)的影响。结果表明,4种合金的体外生物相容性按含1%铜的钴铬合金、含2%铜的钴铬合金、钴铬合金、含3%铜的钴铬合金的顺序递减;其细胞毒性等级都为1级,具有良好的体外生物相容性。     

外科植入物用Ti-5Al-2.5Fe合金的机械性能及生物相容性

在实验室及扩大试验条件下,研究了专门为外科植入物设计的钛合金--Ti-5Al-2.5Fe合金的机械性能及生物相容性.结果表明,Ti-5Al-2.5Fe合金性能优良,价格低廉,生物相容性好,是理想的生物植入材料.     

可模锻钛合金强度高抗蚀性好

美国ATI华昌公司制成了强度与Ti-6Al-4V相当，且可热加工、冷加工的钛合金。这种被称为ATI425合金的成分为Ti-4Al-2．5V-1-5Fe-0．250，其最重要的特点是易于制造和成形。测试表明，这种合金比Ti-6Ak-4V易于制造。     

Ti-6Al-4V基类金刚石生物梯度薄膜的研究进展

Ti-6Al-4V合金作为生物医用材料已广泛应用于临床,但仍存在一些问题:耐磨、耐腐蚀及生物相容性不理想.类金刚石(DLC)具有硬度高、耐磨、生物相容性好等特点.在Ti-6Al-14V基上制备类金刚石生物梯度薄膜不仅能解决这些问题,而且膜基结合力好.本文综述了Ti-6Al-4V基类金刚石生物梯度薄膜的梯度设计、制备工艺和性能研究进展,并对其应用和未来进行了展望.     

热处理对Ti-Nb-Zr合金不同相演变的影响

钛及钛合金具有一系列优良的性能,如高比强度、良好的生物相容性、相对较高的抗疲劳性能及血液相容性,在整形、牙科及心外科作为植人材料得以应用.常用的有Ti-6Al-4V合金和Ti-6A1-7Nb合金,它们相对于人骨有较高的弹性模量,长期植入会向人体内释放有毒离子.而富含β相、模量低且不含有毒元素的Ti-Nb-Zr合金则成为良好的替代合金.     

Ti-6Al-4V合金氮离子注入后在Tyrode’s体液中的耐蚀耐磨性能

Ti-6Al-4V合金注入氮离子后,生物相容性、抗磨损性、抗疲劳性及抗腐蚀性均有所提高,其植入人体后在体液中的耐蚀耐磨性能尤为重要,目前对此研究较少。对Ti-6Al-4V合金进行氮离子注入,并在人工模拟体液Tyrode’s溶液中进行摩擦磨损和电化学试验,研究了注氮Ti-6Al-4V合金的耐蚀耐磨性能。采用扫描电镜观察腐蚀形貌,用其自带的能谱仪分析注氮层的元素组成,采用X射线衍射仪分析注氮层的结构。结果表明:Ti-6Al-4V氮离子注入后表面形成主要由TiC,Ti及少量TiO2组成的膜层,硬度提高,在Tyrode’s溶液中的腐蚀电位升高、极化电阻增大,阳极极极化电流密度降低;在Tyrode’s溶液中摩擦后的注氮Ti-6Al-4V合金的阳极极化电流密度大于未摩擦的,极化电阻减小;Ti-6Al-4V合金氮离子注入后的摩擦系数明显降低,比磨损率减少。     

拥有超精细结构和高成形性能的钛合金

日本JFE支承件公司开发出一种钛合金，其晶粒在2～3μm间，有很好的成形性和出色的疲劳极限。SP-700富β和α＋β钛合金的成分为Ti-4．5Al-3V-2Fe-2Mo。超精细微结构极大地改进了合金的疲劳极限。SP-700的临界冷轧比可达60％～70％，而Ti-6Al-4V只有20％。     

牙科用铸造Ti-15Zr-4Nb-4Ta合金

Ti,Zr,Nb,Ta等金属具有优越的生物相容性,被广泛应用到生物材料中.日本学者Yoshimitsu Okazaki等人研究了铸造Ti-15Zr-4Nb-4Ta合金、Co-Cr-Mo和Ti-6Al-7Nb合金的室温力学性能和疲劳强度,并对Ti-15Zr-4Nb-4Ta合金制作的假牙牙托进行了超过1年的临床观察.     

新型磷镁晶须的体外生物相容性和抗菌性能EI北大核心CSCD

采用RTCA法和Annexin-V/PI双标记法研究了一种新型磷镁晶须的体外生物相容性,采用共培养法研究了抗菌性能。结果表明,磷镁晶须材料的生物相容性随着晶须浓度的提高而降低,晶须浓度小于等于500μg/m L时具有较好的体外生物相容性。当晶须浓度为50μg/m L和200μg/m L时,对成骨细胞几乎没有影响。磷镁晶须的杀菌效能随着浓度的提高而提高,浓度为500μg/m L时对大肠杆菌和金黄色葡萄球菌的杀菌率分别达到96.84%和99.93%,表现出优异的抗菌性能。     

Fabrication of low-cost Ti-1Al-8V-5Fe by powder metallurgy with TiH2 and FeV80 alloy

The low-cost Ti-1Al-8V-5Fe (Ti-185) alloy with a high strength is prepared by cold-compaction-and-sintering powder metallurgy process with low-cost titanium hydride (TiH₂) powders and FeV₈₀ master alloy powders. The use of simple technique process and cheap alloying elements can lead to the cost reduction for titanium alloys. The thermal decomposition of TiH₂-1Al-8V-5Fe is analyzed by thermal gravimetric analyses and differential scanning calorimetry simultaneous thermal analyzer. The shrinkage behavior of TiH₂-1Al-8V-5Fe during the sintering process is employed by the high-sensitivity dilatometer system. The microstructure of sintered Ti-185 consists of β-phase and lamellar α-phase. The results show that the sintered Ti-185 alloys have the relative density of 97.8%, homogeneous composition, and fine grains. The yield strength and the hardness are 1461?MPa and 40.1?±?1.0 HRC (unit of Rockwell hardness), which are better than that of as-cast Ti-185.     

Development of a β-type Ti–12Mo–5Ta alloy for biomedical applications: cytocompatibility and metallurgical aspects

Ti-based biocompatible alloys are especially used for replacing failed hard tissue. Some of the most actively investigated materials for medical implants are the beta-Ti alloys, as they have a low elastic modulus (to inhibit bone resorption). They are alloyed with elements such as Nb, Ta, Zr, Mo, and Fe. We have prepared a new beta-Ti alloy that combines Ti with the non-toxic elements Ta and Mo using a vacuum arc-melting furnace and then annealed at 950 degrees C for one hour. The alloy was finally quenched in water at room temperature. The Ti-12Mo-5Ta alloy was characterised by X-ray diffraction, optical microscopy, SEM and EDS and found to have a body-centred-cubic structure (beta-type). It had a lower Young's modulus (about 74 GPa) than the classical alpha/beta Ti-6Al-4V alloy (120 GPa), while its Vickers hardness remained very high (about 303 HV). This makes it a good compromise for a use as a bone substitute. The cytocompatibility of samples of Ti-12Mo-5Ta and Ti-6Al-4V titanium alloys with various surface roughnesses was assessed in vitro using organotypic cultures of bone tissue and quantitative analyses of cell migration, proliferation and adhesion. Mechanically polished surfaces were prepared to produce unorientated residual polished grooves and cells grew to a particularly high density on the smoother Ti-12Mo-5Ta surface tested.     

Fibroblast Adhesion and Proliferation on a New β Type Ti-39Nb-13Ta-4.6Zr Alloy for Biomedical Application

Cell attachment and spreading on Ti-based alloy surfaces is a major parameter in implant technology. Ti-39Nb-13Ta-4.6Zr alloy is a new β type Ti alloy developed for biomedical application. This alloy has low modulus and high strength, which indicates that it can be used for medical purposes such as surgical implants. To evaluate the biocompatibility and effects of the surface morphology of Ti-39Nb-13Ta-4.6Zr on the cellular behaviour, the adhesion and proliferation of rat gingival fibroblasts were studied with substrates having different surface roughness and the results were also compared with commercial pure titanium and Ti-6Al-4V. The results indicate that fibroblast shows similar adhesion and proliferation on the smooth surfaces of commercial pure titanium （Cp Ti）, Ti-39Nb-13Ta-4.6Zr, and Ti-6Al-4V, suggesting that Ti-39Nb-13Ta-4.6Zr has similar biocompatibility to Cp Ti and Ti-6Al-4V. The fibroblast adhesion and spreading was lower on rough surfaces of Cp Ti, Ti-39Nb-13Ta-4.6Zr and Ti-6Al-4V than on smooth ones. Surface roughness appeared to be a dominant factor that determines the fibroblast adhesion and proliferation.     

Mechanical,forming and biological properties of Ti-Fe-Zr-Y alloys prepared by 3D printing

Biomedical Ti-Fe-Zr-Y alloys were prepared by 3D printing on pure titanium substrate. The influences of Zr on mechanical, forming, and biological properties of the alloys were investigated in detail. The results showed that with increasing the Zr addition, the surface roughness, friction coefficient and worn volume decrease at first and then increase, the lowest values obtained at 5.86 at.% Zr addition. The ultimate compression stress and specific strength gradually decrease. The studied alloys have no cytotoxicity. They can promote the early adhesion and proliferation of cells. The eutectic alloy with 5.86 at.% Zr addition has the best ability of apatite deposition, it exhibits a better comprehensive performance among the studied alloys, which is superior to the Ti_70.5Fe_29.5 and Ti-6Al-4 V alloys.     

Fibroblast Adhesion and Proliferation on a New β Type Ti-39Nb-13Ta-4.6Zr Alloy for Biomedical Application

Cell attachment and spreading on Ti-based alloy surfaces is a major parameter in implant technology. Ti39Nb-13Ta-4.6Zr alloy is a new β type Ti alloy developed for biomedical application. This alloy has low modulus and high strength, which indicates that it can be used for medical purposes such as surgical implants.To evaluate the biocompatibility and effects of the surface morphology of Ti-39Nb-13Ta-4.6Zr on the cellular behaviour, the adhesion and proliferation of rat gingival fibroblasts were studied with substrates having different surface roughness and the results were also compared with commercial pure titanium and Ti-6Al-4V. The results indicate that fibroblast shows similar adhesion and proliferation on the smooth surfaces of commercial pure titanium (Cp Ti), Ti-39Nb-13Ta-4.6Zr, and Ti-6Al-4V, suggesting that Ti-39Nb-13Ta-4.6Zr has similar biocompatibility to Cp Ti and Ti-6Al-4V. The fibroblast adhesion and spreading was lower on rough surfaces of Cp Ti, Ti-39Nb-13Ta-4.6Zr and Ti-6Al-4V than on smooth ones. Surface roughness appeared to be a dominant factor that determines the fibroblast adhesion and proliferation.     

Relationships of strength to composition and phase constitution for three aged beta titanium alloys

X-ray diffraction techniques were used to study properties of three beta titanium alloys in the alpha aged condition. The alloys studied were Beta 111 (Ti-11.5 Mo-6 Zr-4.5 Sn), Beta C (Ti-3 Al-8 V-6 Cr-4 Mo-4 Zr), and 8-8-2-3 (Ti-8 V-8 Mo-2 Fe-3 Al). The volume percentage of alpha phase present and the lattice parameters of both the alpha and beta structures were determined for different ageing treatments. Ultimate tensile strength is related to both alpha content and beta unit cell size in these alloys. However, at high strength levels, beta unit cell size is a more sensitive indicator of tensile strength than percentage of alpha phase. The effects of precipitation hardening mechanisms and alloy partitioning on strengthening are discussed.     

The sintering densification and microstructure evolutions of Ti-1Al-8V-5Fe alloy with Si additions

The cost-efficient Ti-1Al-8V-5Fe-xSi (denoted Ti-185-xSi hereafter, x = 0, 0.15, 0.3, 0.45, 0.6, 0.75) alloys are synthesized by cold compaction and sintering powder metallurgy (PM) technology using TiH₂ and high-pure FeV₈₀ powders. The sintering densification, microstructural evolutions and mechanical behavior of Ti-185-xSi alloys sintered at 1350°C are investigated. The results show that the Si element is favorable to enhance the sintered density of Ti-185 alloys, which should be limited to ≤0.3%. The amount and average size of precipitate Ti₅Si₃ increase in the Ti-185 alloys with increasing Si content. Meanwhile, the Rockwell hardness of Ti-185 alloy also displays an increasing tendency, suggesting the Si element can improve the hardness of Ti-185. The Ti-185–0.15Si alloy possesses a better comprehensive mechanical property of strength (937 ± 8 MPa) and elongation (3.5%). The high-performance Ti-185 alloy is successfully prepared using low-cost FeV₈₀ master alloy with slight Si impurity instead of costly V.     

Microstructural investigation of a rapidly solidified Ti-6Al-4V-1B-0.5Y alloy

A Ti-6Al-4V-1B-0.5Y (wt%) alloy has been prepared by consolidation of the melt-spun alloy fibres. The microstructures of the melt-spun fibre and the consolidated alloy were examined by different techniques. It was found that in the consolidated alloy, titanium boride and yttrium oxide particles have a refined particle size and a uniform distribution in the (+) matrix compared with the microstructure of the same alloy obtained by conventional ingot metallurgy. The boride phase in the consolidated alloy mainly has a needle-shaped morphology and has been identified by electron diffraction to be orthorhombic TiB with a B27 structure, while the yttrium oxide has a cuboidal morphology and has been identified as bcc Y₂O₃. Detailed TEM examination also revealed that yttrium addition has a strong influence on the TiB morphology by comparing the microstructures of Ti-6Al-4V-1B alloys with and without yttrium addition. Under similar processing conditions, the TiB phase in the consolidated alloys without yttrium addition mainly has a nearly equiaxed morphology with a finer particle size, while the TiB phase in the consolidated alloy with yttrium addition will mainly have a needle-shaped morphology. This effect of yttrium addition on the TiB morphology has been discussed in terms of heterogeneous nucleation and the reduced undercooling.     

The effects of micro arc oxidation of gamma titanium aluminide surfaces on osteoblast adhesion and differentiation

The adhesion and proliferation of human fetal osteoblasts, hFOB 1.19, on micro arc oxidized (MAO) gamma titanium aluminide (γTiAl) surfaces were examined in vitro. Cells were seeded on MAO treated γTiAl disks and incubated for 3 days at 33.5 °C and subsequently for 7 days at 39.5 °C. Samples were then analyzed by scanning electron microscopy (SEM) and alkaline phosphatase assay (ALP) to evaluate cell adhesion and differentiation, respectively. Similar Ti-6Al-4V alloy samples were used for comparison. Untreated γTiAl and Ti-6Al-4V disks to study the effect of micro arc oxidation and glass coverslips as cell growth controls were also incubated concurrently. The ALP Assay results, at 10 days post seeding, showed significant differences in cell differentiation, with P values <0.05 between MAO γTiAl and MAO Ti-6Al-4V with respect to the corresponding untreated alloys. While SEM images showed that hFOB 1.19 cells adhered and proliferated on all MAO and untreated surfaces, as well as on glass coverslips at 10 days post seeding, cell differentiation, determined by the ALP assay, was significantly higher for the MAO alloys.     

Microtwin formation in the α phase of duplex titanium alloys affected by strain rate

The effect of tensile strain rate on deformation microstructure was investigated in Ti-6-4 (Ti-6Al-4V) and SP700 (Ti-4.5Al-3V-2Mo-2Fe) of the duplex titanium alloys. Below a strain rate of 10⁻² s⁻¹, Ti-6-4 alloy had a higher ultimate tensile strength than SP700 alloy. However, the yield strength of SP700 was consistently greater than Ti-6-4 at different strain rates. The ductility of SP700 alloy associated with twin formation (especially at the slow strain rate of 10⁻⁴ s⁻¹), always exceeded that of Ti-6-4 alloy at different strain rates. It is caused by a large quantity of deformation twins took place in the α phase of SP700 due to the lower stacking fault energy by the β stabilizer of molybdenum alloying. In addition, the local deformation more was imposed on the α grains from the surrounding β-rich grains by redistributing strain as the strain rate decreased in SP700 duplex alloy.