活性炭吸附废水中铬黑T的研究

以铬黑T模拟废水,采用活性炭作为吸附剂,利用SEM对活性炭进行了表征。研究了吸附时间、废水浓度、废水pH值及吸附温度等因素对吸附效果的影响。根据这些因素的影响规律设计正交试验。结果表明佳工艺条件为:废水溶液浓度为0.03 mg/mL,吸附时间为70 min,溶液pH值为4.00,吸附温度为35℃。并根据这些因素的影响规律对活性炭吸附法处理铬黑T废水的吸附脱色机理进行了探讨。     

铬黑T指示液抗聚性研究

对作用于铬黑T的不同阻聚剂进行筛选试验和性能分析。结合可见吸收光谱和容量分析,说明不同阻聚剂对铬黑T指示液的作用结果:无水乙醇-醋酸乙烯酯体系集阻聚、解聚、抗氧化、助溶、增敏于一体,30%醋酸乙烯酯作用下的铬黑T指示液显色灵敏、保存时间长,其阻聚能力明显优于丙酮、丙三醇和三乙醇胺。     

铬黑T作为络合滴定指示剂稳定性的研究

用络合滴定法和分光光度法对铬黑T在三乙醇胺和无水乙醇溶液中的稳定性进行了研究,认为铬黑T在三乙醇胺和无水乙醇溶液中作为络合滴定指示剂至少在一年中有效。     

利用活性炭处理铬黑T实验室废液的试验研究

选用活性炭对低浓度铬黑T实验室模拟废液进行脱色处理,通过活性炭用量、活性炭浓度,搅拌时间、pH值等变量,进行脱色率的试验.通过设计正交实验选出的最佳脱色条件为:活性炭用量1000mg/L,搅拌时间100min,铬黑T质量浓度15mg/L,pH为2.试验证明在此条件下进行脱色,脱色率都达到90%以上.     

固态铬黑T指示剂应用条件的研究

铬黑T (EBT),作为指示剂,在配位滴定中被广泛应用。液态铬黑T指示剂存在稳定性不高的问题,而固态铬黑T指示剂因用量不易控制,平行测定的重现性较差。以自来水中总硬度的测定为研究体系,通过与液体指示剂进行对比,探索适宜的固态铬黑T指示剂应用的条件和方法。     

电絮凝处理模拟铬黑T染料废水

电絮凝(EC)具有成本低、操作简单、无需添加药剂、污泥少以及无二次污染等优点。采用电絮凝技术处理模拟铬黑T(EBT)染料废水，研究了不同电解质及其浓度、电流密度、初始pH以及溶液初始浓度对染料废水的脱色率和化学需氧量(COD)去除率的影响。结果表明，该技术对染料废水的色度和COD的去除均有良好的去除效果，在纯Al板为电极，染料废水浓度为100 mg/L，极板间距为15 mm,NaCl浓度为0.75 g/L，电流密度为10 mA/cm²，溶液初始pH为6的实验条件下，电解20 min，脱色率可达97.5%,COD去除率为61.3%。电絮凝过程中不仅可以产生有较强吸附作用的絮体，还可以产生能够破坏染料分子中发色基团的强氧化性物质，从而降低废水的色度和COD含量。因此，电絮凝技术在染料废水的处理方面有一定的应用前景。     

高铁酸钾降解铬黑T和铬蓝黑R的研究

采用高铁酸钾对水溶性铬黑T和铬蓝黑R废水进行氧化脱色研究,考察高铁酸钾用量、反应温度和染料质量浓度对脱色率的影响。结果表明,高铁酸钾能够有效去除染料废水的色度,高铁酸钾用量和反应温度均存在最佳值。铬黑T和铬蓝黑R的质量浓度低于100mg/L时,脱色率均可达到87%以上。     

超声降解铬黑T及亚甲蓝的实验研究

尝试采用超声波技术对铬黑T及亚甲基蓝两种模拟染料废水进行降解实验。以脱色率为指标,分别考察pH值、超声功率强度、超声时间和温度等因素对两种模拟染料废水脱色作用的影响。结果表明:超声波技术对铬黑T及亚甲基蓝两种模拟染料废水具有一定的脱色能力,随着pH值的增大,脱色能力减弱。常温下,在pH=1.79附近、超声时间120min,超声功率0.7kw时,亚甲基蓝模拟染料废水的脱色率为27.60%,铬黑T模拟染料废水的脱色率为20.33%。进一步地采用超声波与水合二氧化锰联用技术,可以极大提高有机染料的降解效率,水合二氧化锰投加量为40mg/L时,亚甲基蓝模拟染料废水的脱色率达到92.48%。     

铬黑T分光光度法测定微量Ni（Ⅱ）

本文研究了铬黑T（EBT）与镍（）的显色反应条件,建立了新的测定微量Ni（）的较高灵敏度分光光度法。实验结果表明,pH10.0 H2B4O7-KCl-NaOH缓冲介质中,镍（）与EBT发生灵敏的显色反应,生成紫罗兰色的配合物。配合物最大吸收波长为532 nm,表观摩尔吸光系数ε为4.01×104L.mol-1.cm-1,Ni2＋的质量浓度在0~30μg/25mL范围内遵循比尔定律。结果表明,本方法用于微量镍（）的测定,结果令人满意。     

聚硅酸铝铁絮凝剂对铬黑T废水的脱色处理

本文研究了聚硅酸铝铁絮凝剂的制备及应用性能,确定了制备和絮凝处理的最佳条件,该絮凝剂用于铬黑T废水处理,脱色率达到97%以上,絮凝效果明显优于聚硅酸铝和聚硅酸铁,是一种性能更优的无机高分子复合絮凝剂。     

孔雀石绿与蛋白质相互作用的电化学行为的研究

在pH 4.7的NaAc-HAc缓冲液中,孔雀石绿(MG)能与牛血清白蛋白相互作用形成复合物,使孔雀石绿在-0.72 V(vsSCE)处有一极谱还原峰峰电流下降,在最佳条件下,峰电流的下降值同牛血清白蛋白(BSA)的浓度在0.5～20 mg/L范围内呈线性关系,其线性回归方程为Δip(nA)=-4.78+41cBSA(mg/L),相关系数r=0.996(n=10),检出限为0.4 mg/L.可将该方法应用于血清样品的测定,结果满意.     

壳聚糖富集分离镉黑T褪色光度法测定痕量锰的研究

常温下,在pH=8的硼酸-氯化钾-氢氧化钠介质中,Mn(Ⅶ)能显著的氧化铬黑T褪色,且褪色程度随Mn(Ⅶ)量变化呈线性关系,据此建立了测定微量锰的新方法。实验测定波长为620 nm,表观摩尔吸光系数为1.31×105L/(mol.cm),该方法的线性测定范围为0～10μg/25 mL,相关系数为r=0.999 7。方法简便、快速、准确,精密度和灵敏度高;采用壳聚糖富集分离和EDTA掩蔽,方法在用于测定钢样中锰的含量时,结果非常满意。     

DNA与几种表面活性剂的作用

本文介绍了阳离子表面活性剂,两性离子表面活性剂,非离子表面活性剂与DNA的相互作用.通过共振光散射(RLS),荧光显微镜,浊度法等方法测定表面活性剂与DNA之间的作用.试验发现:阳离子型表面活性剂分子可能通过静电作用和疏水作用,以DNA分子为模板在其表面形成了聚集,这种聚集增强了体系的RLS响应.当pH值低时,两性表面...     

有机氯农药与牛血清白蛋白作用机理研究

利用荧光光谱法研究了5种有机氯农药（OCPs） δ-BHC、α-氯丹、艾氏剂、o,p＇-DDT、HCB和牛血清白蛋白（BSA）的相互作用机理.280 nm波长下,OCPs可使BSA色氨酸残基发生荧光猝灭现象.通过光谱数据,绘制了Stern-Volmer曲线,测定了平衡常数和25,37℃下OCPs-BSA的结合常数.结果显示,5种OCPs猝灭蛋白质的过程均为熵驱动的静态猝灭过程,通过较弱的疏水作用力与蛋白质相互结合,结合点位数近似为1,这种结合作用会随着温度的升高而增强.     

Studies on the interaction of CdTe QDs with bovine serum albumin

BACKGROUND: Quantum dots (QDs) have attracted much attention in biological and medical applications. In particular, the interaction of QDs with bovine serum albumin (BSA) is crucial, and has been systematically investigated by various spectroscopic techniques under the physiological conditions. RESULTS: The effects of ionic strength and pH on the interaction of CdTe QDs with BSA were studied by changing NaCl concentration and pH in mixed solution and making fluorescence spectroscopic measurements. The Stern‐Volmer quenching constant (K_a) of different ionic strength and pH were calculated, and information on the structural features of BSA were discussed by means of circular dichroism (CD) spectrum. CONCLUSION: Both fluorescence (FL) and circular dichroism (CD) results indicated that hydrophobic and electrostatic interactions play a major role in the binding reaction, and the nature of quenching is static, resulting in forming QDs‐BSA complexes. Copyright © 2012 Society of Chemical Industry     

Electrochemical characterization of poly(eriochrome black T) modified glassy carbon electrode and its application to simultaneous determination of dopamine, ascorbic acid and uric acid

A polymerized film of eriochrome black T (EBT) was prepared on the surface of a glassy carbon (GC) electrode in alkaline solution by cyclic voltammetry (CV). The redox response of the poly(EBT) film at the GC electrode appeared in a couple of redox peak in 0.1 M hydrochloride and the pH dependent peak potential was −55.1 mV/pH which was close to the Nernst behavior. The poly(EBT) film-coated GC electrode exhibited excellent electrocatalytic activity towards the oxidations of dopamine (DA), ascorbic acid (AA) and uric acid (UA) in 0.05 mM phosphate buffer solution (pH 4.0) and lowered the overpotential for oxidation of DA. The polymer film modified GC electrode conspicuously enhanced the redox currents of DA, AA and UA, and could sensitively and separately determine DA at its low concentration (0.1 μM) in the presence of 4000 and 700 times higher concentrations of AA and UA, respectively. The separations of anodic peak potentials of DA-AA and UA-DA reached 210 mV and 170 mV, respectively, by cyclic voltammetry. Using differential pulse voltammetry, the calibration curves for DA, AA and UA were obtained over the range of 0.1-200 μM, 0.15-1 mM and 10-130 μM, respectively. With good selectivity and sensitivity, the present method provides a simple method for selective detection of DA, AA and UA in biological samples.     

荜茇宁与牛血清白蛋白相互作用的研究

用荧光光谱及紫外光谱法研究生理条件下荜茇宁与生物大分子牛血清白蛋白(BSA)的相互作用.实验结果表明,荜茇宁与BSA形成基念复合物从而猝火BSA的内源性荧光,猝灭原因主要为静态猝灭和非辐射能量转移.根据Forster 偶极-偶极非辐射能量转移理论,确定荜茇宁与BSA有较强的相互作用,可以被蛋白质所储存和运输.荜茇宁与B...     

对硝基氯苯与牛血清白蛋白的相互作用研究

在模拟动物体生理条件下,用荧光光谱、三维荧光光谱、同步荧光光谱、紫外-可见吸收光谱等方法研究了在不同温度下,对硝基氯苯(PCNB)与牛血清白蛋白(BSA)结合反应的光谱行为。试验发现,PCNB对BSA有较强的荧光猝灭作用。用Stern-Volmer和Lineweaver-Burk方程及热力学方程分别处理试验数据,发现BSA与PCNB发生反应生成了新的复合物,属于静态荧光猝灭,得到了它们相互作用的生成常数KLB(1.557×104L/mol)、热力学参数(ΔHθ=-89.962kJ/mol,ΔSθ=-217.98J/K,ΔGθ=-23.93kJ/mol)和结合位点数(1.080)等。位点竞争实验结果显示PCNB与BSA的作用位置主要在BSA的SiteⅠ(Sub-domainⅡA)位。证明二者主要靠氢键和范德华力结合,同时用三维荧光光谱及同步荧光光谱法探讨了PCNB对BSA构象的影响,为研究PCNB的毒性和生物学效应提供了重要信息。     

Competitive interaction of polyanions and anionic dye with bovine serum albumin

The binding of anionic dye, p-(2-amino-6-sulfonyl-8-naphthylazo)benzene sulfonic acid disodium salt (ASANA) to bovine serum albumin (BSA) at pH 7.5 has been studied by spectrophotometric techniques. The values of the dissociation constants were obtained with the use of the Benesi-Hildebrand equation for ASANA. Competitive binding of polyanions, sodium poly(styrene sulfonate) (PSSNa), potassium poly(vinyl sulfonate) (PVSK), poly(acrylic acid) (PAA), and poly(methacrylic acid) (PMAA) and anionic dye to BSA was evaluated through the variations in the different spectra of BSA-dye-polymer systems.     

Fluorescence study of interaction between an anionic conjugated polyelectrolyte and bovine serum albumin

The interaction between an anionic conjugated polyelectrolyte, poly[9,9-bis(3′-butyrate)fluoren-2,7-yl] sodium (BBS-PF), and bovine serum albumin was investigated by fluorescence spectroscopy. The emission of BBS-PF was effectively quenched by BSA with a quenching constant K _SV of 3.1 × 10⁷ L/mol when BSA was at nanomolar concentrations, but the emission increased when the concentration of BSA was at micromolar level. The excitation band of BBS-PF blue-shifted when the emission was quenched where the negatively charged BSA induced the aggregation of BBS-PF, yet the excitation band of BBS-PF red-shifted when the emission increased where the BSA acted as a surfactant and formed hydrophobic interaction with BBS-PF. BBS-PF could also quench BSA through energy transfer by resonance with a quenching constant K _SV of 1.1 × 10⁶ L/mol. The emission band changes of BSA reflected the conformation transitions of BSA from class II to class I and the binding of BBS-PF with BSA made the BSA more folded.