液相色谱-质谱法测定辣椒中的苏丹红Ⅰ

建立了液相色谱-质谱法测定辣椒中的苏丹红Ⅰ,用乙腈提取样品中的目标物,采用ACQUITY UPLCTM BEH C18柱,以乙腈:水=90:10为流动相,进行液相色谱.质谱检测.其线性范围为0.01μg/mL～10μg/mL:线性回归系数为0.9997;检出限为0.1μg/mL:回收率为87.9%～95.4%.     

液相色谱-质谱/质谱法测定乳与乳制品中的三聚氰胺

目的建立乳与乳制品中三聚氰胺的液相色谱-质谱/质谱(LC-MS/MS)测定方法。方法样品用三氯乙酸溶液和乙腈提取,经阳离子交换固相萃取柱(SPE)净化后,用液相色谱-质谱/质谱法测定和确证,外标法定量。结果在0.01～0.50μg/ml范围内呈线性相关,相关系数为0.999 2,方法测定低限(LOQ)为0.01 mg/kg。在0.01～0.10μg/ml添加浓度范围内,方法回收率为80.4%～107.4%,相对标准偏差(RSD)≤9.4%。结论本方法操作简便,灵敏度、准确度、精密度可满足乳与乳制品中三聚氰胺的检测要求。     

液相色谱-串联质谱测定乳制品中乳酸链球菌素

建立液相色谱-串联质谱法用于测定乳酸链球菌素的含量。PLRPS-S为色谱柱。样品经提取净化后,用超高效液相色谱分离后采用电喷雾串联质谱进行定性和定量检测。线性范围为0～1 000μg/L,线性相关系数r大于0.99,方法检出限为0.2 mg/kg,在加标浓度为0.2～5.0 mg/kg条件下的回收率为89.01%～104.52%,精密度为1.99%～8.77%。方法简单、灵敏、准确、精密,满足检测需求。     

高效液相色谱和高效液相色谱-离子阱质谱测定花生、玉米和大米中黄曲霉毒素方法比较

比较花生、玉米和大米中黄曲霉毒素(AFT)测定的两种分析方法。样品经70%甲醇溶液(含NaCl 4g/100mL)提取,自制免疫亲和柱净化。洗脱液一分为二,一份经正己烷、三氟乙酸衍生后用配有荧光检测器的液相色谱检测;另一份无需衍生用液相色谱-串联离子阱质谱检测。液相色谱测定的AFB1、AFB2、AFG1和AFG2的检出限(RSN=3)分别为0.5～1.5μg/kg,相关系数在0.9979～0.9999,加标回收率50.29%～126.54%;液相色谱-串联质谱测定的AFB1、AFB2、AFG1和AFG2的检出限分别为0.1～0.2μg/kg;相关系数在0.9993～0.9998,加标回收率50.38%～121.27%。结果表明液相色谱-离子阱质谱法无论在选择性还是在灵敏度上都优于高效液相色谱。     

液相色谱-质谱/质谱法测定纺织品中全氟己烷磺酸

建立高效液相色谱-质谱/质谱法测定纺织品中的全氟己烷磺酸(PFHxS)的方法。样品经甲醇超声提取,提取液经C₁₈色谱柱分离,以多反应监测模式进行定性和定量。此方法检出限为0.3μg/kg,定量限为1.5μg/kg,平均加标回收率为88.13%～93.04%,相对标准偏差为2.05%～4.37%。本方法适用于纺织品中PFHxS的快速测定。     

QuEChERS-高效液相色谱-质谱/质谱法同时测定水产品中磺胺类和氯霉素类药物

目的 建立QuEChERS-高效液相色谱-质谱/质谱法同时测定水产品中磺胺类药物和氯霉素类药物含量的分析方法。方法 样品用乙腈提取, 经QuEChERS方法去脂净化后, 用甲醇和0.02%的甲酸水溶液作为流动相进行梯度洗脱, Waters Atlantis T3色谱柱进行色谱分离, 高效液相色谱-质谱/质谱电喷雾电离多反应监测正负离子模式测定, 内标法定量。结果 23种药物标准曲线的相关系数均大于0.99, 加标平均回收率为61.4%~116.2%。结论 本方法操作简单、方便高效, 可同时测定水产品中磺胺类和氯霉素类药物, 适合于实验室多项目的快速检测。     

超高效液相色谱-串联质谱法测定茶叶中的百草枯

文章建立了一种利用超高效液相色谱-串联质谱法测定茶叶中百苹枯含量的方法.茶叶样品使用甲醇-0.1 mol/L盐酸溶液(3:7,V/V)提取,经Poly-sery MCX柱净化后用超高效液相液相色谱-串联质谱进行测定.在5～100μg/L浓度范围内,百草枯的线性良好(R2=0.9991),方法定量限为0.01 mg/kg...     

改进的食品中三聚氰胺的高效液相色谱串联质谱检测法

高效液相色谱串联质谱法是测定三聚氰胺公认的标准方法,具有灵敏度高、精密度好等特点.本研究对国家标准GB/T22388-2008中液相色谱串联质谱法进行了改进,对于液态奶、奶粉和鲜蛋基质,采用50%乙腈水溶液超声提取30min,提取液经过离心处理后,用Agela ASB-C18色谱柱(150×2.1min,5μm)分离,乙腈和10mmol/L乙酸铵作为流动相(5:95),用串联质谱在多反应监测模式下定量检测.方法定量限为0.0lmg/kg,线性范围为0.01～0.5mg/L,相关系数r2>0.999.平均回收率为65%-80%,相对标准偏差为2.159%-6.68%(n=6).     

超高效液相色谱-四极杆/静电场轨道阱高分辨质谱法快速筛查及定量分析保健品中29种非法添加物

目的 建立超高效液相色谱-四极杆/静电场轨道阱质谱法(ultra performance liquid chromatographyquadrupole/electrostatic field orbitrap high resolution mass spectrometry, UPLC-Q-Orbitrap HRMS)快速筛查及定量分析保健品中常见29种非法添加物的方法。方法 样品用甲醇进行超声提取,经去离子水稀释后用UPLC-Q-Orbitrap HRMS进行测定。色谱柱选用HSS T3(1.8μm)柱,质谱采用全扫描/数据依赖二级扫描模式,通过一级质谱和二级质谱信息建立的数据库进行高通量筛查及定量分析。对建立的方法进行方法学考察,幵对市售保健品样品进行筛查测定。结果 各组分在0.1～500 ng/mL浓度范围内具有良好的线性,各化合物的检出限为0.3～20 ng/g,回收率为68.2%～105.3%,相对标准偏差为0.4%～11.3%,能够满足日常检测的要求。结论该方法简单快捷,准确度高,可用于检测保健品中非法添加物的日常监管工作。     

UPLC-MS/MS法测定生菜中氟吡菌酰胺及其代谢物残留量

目的 建立超高效液相色谱-串联质谱法测定生菜中氟吡菌酰胺及其代谢物2-(三氟甲基)苯甲酰胺的方法。方法 采用QuEChERS技术,用乙腈对生菜样品进行萃取,C18净化剂净化后,用含0.1%甲酸的乙酸铵溶液-乙腈在BEH C18色谱柱上进行洗脱分离,质谱检测器测定。结果 氟吡菌酰胺和2-(三氟甲基)苯甲酰胺在1.0~50.0 ng/mL质量浓度范围内,质量浓度与其质谱响应强度之间呈线性关系,相关系数不小于0.9996;检出限为0.25 μg/kg,定量限为0.1 μg/kg;回收率平均值在94.6%~97.2%,相对标准偏差为1.3%~4.5%。结论 所建超高效液相色谱-串联质谱法灵敏度高、精密度好、准确度高、前处理简便,可为生菜农药残留监测及氟吡菌酰胺农药残留消解动态分析提供技术支撑。     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the     

Chinese CTP Market

According to Printing and Printing Equipment Industries Association of China（PEIAC）＇s statistics to the plate manufucturer in China, in 2013, the actual offset plate production has reached 346 million square meters in China. Among them, the CTP production volume was 245 million square meters, up by 11% than that of last year; the total sales of the CTP plate was 239 million square meters, up by 13%.     

Preparatory Work of Print China 2015 is Going Smoothly

正Held at Guangdong Modern International Exhibition Center,Print China 2015 will cover 7exhibition halls,besides the original Hall No.3,4,5,6,7,the newly built F zone of Hall 3 will be used too.The total area will be140,000 square meters.Hall 3:Offset and large printing equipment,package printing equipment,post press