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14-3-3 proteins form a family of highly conserved proteins which are present in all eukaryotic organisms investigated, often in multiple isoforms, up to 13 in some plants. They interact with more than 200 different, mostly phosphorylated proteins. The molecular consequences of 14-3-3 binding are diverse: this binding may result in stabilization of the active or inactive phosphorylated form of the protein, to a conformational alteration leading to activation or inhibition, to a different subcellular localization, to the interaction with other proteins or to shielding of binding sites. The binding partners, and hence the 14-3-3 proteins, are involved in almost every cellular process and 14-3-3 proteins have been linked to several diseases, such as cancer, Alzheimer's disease, the neurological Miller-Dieker and spinocerebellar ataxia type 1 diseases and bovine spongiform encephalopathy (BSE). The yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe both have two genes encoding 14-3-3 proteins, BMH1 and BMH2 and rad24 and rad25, respectively. In these yeasts, 14-3-3 proteins are essential in most laboratory strains. As in higher eukaryotes, yeast 14-3-3 proteins bind to numerous proteins involved in a variety of cellular processes. Recent genome-wide studies on yeast strains with impaired 14-3-3 function support the participation of 14-3-3 proteins in numerous yeast cellular processes. Given the high evolutionary conservation of the 14-3-3 proteins, the experimental accessibility and relative simplicity of yeasts make them excellent model organisms for elucidating the function of the 14-3-3 protein family. 相似文献
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E. Mazzag L. Nagymajtenyi E. Huszta I. Desi R. Macholz 《Molecular nutrition & food research》1991,35(3):309-316
The toxic effects of the γ-hexachlorocyclohexane metabolite γ-pentachlorocyclohexene (γ-PCCH) were studied by acute and subacute (6 weeks) experiments. The investigations included cerebral convulsibility with chemoshock (Tetrazolium), reactivity with hot plate method, the learning ability with learning tests, and peripheral nervous activity (EMG). Nociceptive reaction time was not influenced, the learning process (6 weeks) was inhibited by γ-PCCH. The conduction velocity of the peripheral nerve was decreased. At the end of the 6th week liver enlargement was found. 相似文献
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Yeast 14-3-3 proteins. 总被引:2,自引:0,他引:2
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《中国眼镜科技杂志》2020,(7)
正论坛、公益、科普,微信、微博、抖音……横向观察社会各界在爱眼日期间的主题活动,我们能看出围绕着"普遍的眼健康"这一主题,医院、学校、眼镜企业、行业及协(商)会等是如何开展与自身职能相关、自己最为擅长的活动的。而纵览历年爱眼日的主题,回顾企业和单位每年活动的变化,我们也能动态且辩证地感受到,随着社会生活、行业企业的发展,爱眼日的主题有了显著的变化。 相似文献
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Bun-Ichiro Ono Nobuya Ishii Kazuhide Naito Shin-Ichi Miyoshi Sumio Shinoda Sumiyo Yamamoto Shinji Ohmori 《Yeast (Chichester, England)》1993,9(4):389-397
Purification of Saccharomyces cerevisiae cystathionine γ-lyase (γ-CTLase) was hampered by the presence of a protein migrating very close to it in various types of column chromatography. The enzyme and the contaminant were nevertheless separated by polyacrylamide gel electrophoresis. N-terminal amino acid sequence analysis indicated that they are coded for by CYS3(CYI1) and MET17(MET25), respectively, leading to the conclusion that CYS3 is the structural gene for γ-CTLase and that the contaminant is O-acetylserine/O-acetylhomoserine sulfhydrylase (OAS/OAH SHLase). Based on these findings, we purified γ-CTLase by the following strategy: (1) extraction of OAS/OAH SHLase from a CYS3-disrupted strain; (2) preparation of antiserum against it; (3) identification of a strain devoid of the OAS/OAH SHLase protein using this antiserum; and (4) extraction of γ-CTLase from this strain. Purified γ-CTLase had cystathionine γ-synthase (γ-CTSase) activity if O-succinylhomoserine, but not O-acetylhomoserine, was used as substrate. From this notion we discuss the evolutional relationship between S. cerevisiae γ-CTLase and Escherichia coli γ-CTSase. 相似文献
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Montserrat Pinent Alberto E. Espinel Marco Antonio Delgado Isabel Baiges Cinta Bladé Lluís Arola 《Food chemistry》2011
Isoflavones are widely consumed and they have been attributed beneficial effects. We have explored how genistein, daidzein and equol affect the adipocyte functions of glucose uptake and the secretion of inflammatory molecules in 3T3-L1 adipocytes inflamed with TNF-α. 相似文献
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空中涂鸦3D物体缤纷呈现在经过一年多的技术研发之后,英国WobbleWorks玩具公司最近生产出世界上第一款具有3D打印功能的3Doodler笔。笔的长度180毫米,直径24毫米,重量200克左右,使用万能电源,工作条件为110V或240V。如同3D打印机一样,它也用ABS或PLA塑料当作“墨水”。每只3D涂鸦笔都配备一包这样的“墨水”,里面有10只300毫米长的塑料,每只可创作大约3.4米的3D作品。ABS是最常见的塑料之一,用于很多塑料制品。“生物塑料”PLA来自玉米,可降解并且熔点比ABS要低。从笔头喷出的塑料虽然可接触,但由于笔尖处温度高达270℃,因此使用时一定不要触碰笔尖。 相似文献
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Enzymatic hydrolysis of proteins and fractionation of hydrolysates is a route of diversifying their functional properties. Chymotryptic hydrolysis of different sulphur-rich gliadins (α/β- and γ-types), major wheat storage proteins, was studied. The peptides formed in the course of digestion were characterised by polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate (SDS-PAGE) and reversed-phase high performance liquid chromatography (RP-HPLC). With reference to previous work, a general scheme of degradation was assessed for γ-gliadins. Limited hydrolysis released two types of polypeptides, comprising respectively the repetitive and the non-repetitive moieties of the protein. In spite of strong sequence homologies between the two groups of sulphur-rich gliadins, it was not possible to prepare similar peptide fractions from α/β-gliadins. They were more resistant to hydrolysis and the region where the two domains merge appeared inaccessible to chymotrypsin. Restricted accessibility of cleavage sites was attributed to the less expanded conformation of α/β-type than γ-type gliadins. A first step of scaling-up was performed. This offers opportunities to prepare functional peptides from wheat storage proteins. 相似文献
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Ty3/gypsy retrotransposons are a widespread group of eukaryote mobile genetic elements. They are similar in structure to, and may be ancestors of, the vertebrate retroviruses. Here we describe the first Ty3/gypsy retrotransposons from the pathogenic yeasts Candida albicans and Candida dubliniensis, which we refer to as Tca3 and Tcd3, respectively. Tca3 was first identified in a variety of strains as an element lacking a large part of its coding region. Comparative analyses between C. albicans and C. dubliniensis allowed us to identify the closely related full-length Tcd3 element, and, subsequently, the full-length Tca3 elements. The full-length versions of Tca3 and Tcd3 are broadly similar in structure to other Ty3/gypsy elements, but have several features of special interest, e.g. both elements appear to have a novel mechanism for priming minus-strand DNA synthesis, probably involving conserved secondary structures adjacent to the 5' LTRs. Also, while closely related to each other, the two elements appear to be fairly distantly related to other known Ty3/gypsy-like elements. Finally, the occurrence of the internally deleted forms of Tca3 in many strains raises interesting questions concerning the evolution of these transposable elements in Candida and the evolution of Candida itself. The sequences reported in this paper have been assigned GenBank Accession Nos AF499463, AF499464 and AF510498. 相似文献