柞蚕蛹超氧化物歧化酶的提取及性质的研究初探

从柞蚕蛹中分离铜锌超氧化物歧化酶（Cu，Zn-SOD），并对其部分性质进行研究。用氯仿-乙醇除杂蛋白质、丙酮沉淀、热变性、DE-32柱色谱等方法，对柞蚕蛹Cu，Zn-SOD进行分离纯化。结果得Cu，Zn-SOD干粉9．5mg；比活10116．7U／mg pro．；活力回收55．9％；纯化倍数为360．7。该酶最大吸收波长258nm；pH在5～9范围内基本稳定；在40℃～ 60℃内保温30min酶活基本不变；H2O2对此酶有明显的抑制作用。柞蚕蛹含有丰富的Cu，Zn-SOD。     

马尾松毛虫蛹壳中甲壳素的提取研究

从化害为宝的角度，对马尾松毛虫“蛹壳”中的甲壳素进行了提取研究。结果表明，“虫蛹壳”中甲壳素提取的最佳条件为：（1）脱矿物质：盐酸浓度为5％，处理温度为28℃，处理时间为20h；（2）脱有机物质：NaOH浓度为6％。处理温度为70℃，处理时间为10h；（3）脱色：H2O2浓度为11％，处理时间为2．5h，处理温度为85℃。在此条件下所得甲壳素产品为白色片状固体，其水分含量为8．23％，灰分为0．99％，含氮量为6．84％。所得甲壳素产品达到食品级甲壳素标准。     

酶法从脱脂米糠中提取蛋白质

探讨了糖酶、蛋白酶及二者配合对脱脂米糠中蛋白质的提取条件及效果。糖酶中戊聚糖复合酶的效果最好，其最佳提取条件为：pH值3．5、温度50℃、加酶量0．9％、提取时间3．5h；蛋白酶中碱性蛋白酶的效果最好，最佳提取条件为：pH值8．0、温度45℃、加酶量0.9％、提取时间3．5h。戊聚糖复合酶与碱性蛋白酶配合使用，脱脂米糠中蛋白质的提取率可达到91．6％。     

几种饵料对黑广肩步甲诱杀效果研究

主要论述了不同饵料对黑广肩步甲的诱杀效果对比。通过对试验结果的统计和分析可以看出,用柞蚕蛹粉制成的毒饵诱杀步甲死亡率达90．0％,比以柞蚕幼虫作毒饵的对照区高11．11％,在α=0．05水平上差异显著。另外柞蚕蛹粉与黄瓜、西红柿组合后对步甲诱杀率分别比混配前高14．98％和15．63％,在α=0．01水平上差异显著。试验表明柞蚕蛹粉对罴广肩步甲有很强的引诱效果。     

大豆低聚糖活性炭脱色试验

以低温脱脂豆粕粉为原料，用50％乙醇作提取溶剂，提取豆粕粉中的低聚糖成分。以活性炭为脱色剂，进行脱色试验，试验结果表明：脱色的最佳工艺条件为活性炭用量1．5％，脱色温度60℃，pH值3．5，脱色时间60min，脱色率为30．92％。     

酸性醇浸渍法提取魔芋中的总生物碱

研究了用酸性醇浸渍法提取魔芋中总生物碱的方法和条件。结果表明，提取魔芋中总生物碱较佳的工艺条件是：在pH值为2～3的酸性醇溶液中，水浴温度为5512左右，提取3．5h，总生物碱的含量可达0．20％～0．28％。     

蚕蛹系列产品的开发利用

在干桑蚕未脱脂的蚕蛹中,其粗脂肪含量高达25～30％、粗蛋白含量高达55～60％、甲壳质含量为2～3％;在干柞蚕蛹中,粗脂肪含量高达27.8％,粗蛋白含量高达57％、甲壳质含量为2.8％。这些物质都各有特色,均可分别加以利用开发。特别是困难的经济效益差的制丝厂,将蚕蛹当作猪饲料、把蛹渣当作肥料,低价一卖了之,实在太可惜!这是一种严重的资源浪费! 1 整粒蚕蛹的开发利用: (1)鲜蚕蛹开发成“蚕蛹罐头”等食用品     

刺梨总黄酮的含量测定及资源利用

目的：测定刺梨中总黄酮的含量，为进一步开发利用贵州丰富的刺梨资源提供科学依据。方法：正交设计优选刺梨中总黄酮的提取条件，用芦丁作对照品，三氯化铝作显色剂，测定波长在420nm，比色法测定刺梨中总黄酮含量。结果：优选提取条件是：用65％乙醇提取4h，每次提取体积100ml，提取一次。测定刺梨中总黄酮的含量1，2，3号样分别为0．773％，0．874％，0．893％。结论：优选提取方法效率高，含量测定方法操作简便，准确，稳定性好。     

酶解脱脂虾粉制备多肽最佳工艺条件的研究

对酶法水解脱脂虾粉制备多肽的工艺进行了研究，试验以水解度(DH/%)和酸溶性肽得率(YASP／％)为指标，确定了用胰蛋白酶—木瓜蛋白酶混合酶法水解脱脂虾粉制取多肽的最佳酶解工艺条件为：胰蛋白酶—木瓜蛋白酶的酶量比为1：2(W／W)，总酶量为6000U，脱脂虾粉底物浓度为4％，pH7．0，温度60℃，反应时间6h的条件下，可使水解度和酸溶性肽得率分别达到25．91％和38．46％。     

灰树花乳饮料生产工艺的研究

将脱脂乳和灰树花为原料提取的菌汁相混合，经杀菌后接种双歧杆菌与普通乳酸菌发酵、再经调配后制成乳饮料，通过试验确定最佳工艺条件及配方：灰树花菌汁：脱脂牛乳=2：1（v／v），采用婴儿双歧杆菌为双歧发酵菌种，42℃发酵20h；混合发酵的最佳工艺条件为：普通乳酸菌添加量为3％，41℃混合发酵3h；添加海藻酸钠0．06‰、CMC0．08‰、果胶．05‰的复合稳定剂，蔗糖5％，混合发酵液40％，饮料的酸度（用柠檬酸调酸）为pH4．2，产品酸甜适中，具有灰树花独特的清香。     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the     

Chinese CTP Market

According to Printing and Printing Equipment Industries Association of China（PEIAC）＇s statistics to the plate manufucturer in China, in 2013, the actual offset plate production has reached 346 million square meters in China. Among them, the CTP production volume was 245 million square meters, up by 11% than that of last year; the total sales of the CTP plate was 239 million square meters, up by 13%.     

Preparatory Work of Print China 2015 is Going Smoothly

正Held at Guangdong Modern International Exhibition Center,Print China 2015 will cover 7exhibition halls,besides the original Hall No.3,4,5,6,7,the newly built F zone of Hall 3 will be used too.The total area will be140,000 square meters.Hall 3:Offset and large printing equipment,package printing equipment,post press