Menstrual cycle and social behavior in vervet monkeys

We assessed the relationship between social behavior and the menstrual cycle in 11 adult female vervet monkeys (Cercopithecus aethiops sabaeus) living in an established, stable social group. The findings indicated that fluctuations in ovarian steroids are accompanied by behavioral changes in vervet monkeys. A significant increase in aggressive action, avoidance of social overtures, and retreats from threat occurred during the late luteal phase. However, the social environment can greatly affect behavior independent of the phase of the menstrual cycle. The 10 nondominant (or subordinate) individuals not only exhibited behavioral changes across their own menstrual cycles, but also were responsive to the dominant female's cycle. During the dominant female's late luteal phase, subordinate females significantly increased aggression and decreased social activity. Some of behavioral patterns in female vervet monkeys are therefore relatively independent of direct hormonal modulation and support the contention of the dominant female as the driving force for behavioral changes related to aggression and social interaction. The differential effect of hormones and social status and other environmental factors on behavior has not been critically evaluated in human studies of the premenstrual syndrome. The present study suggests that it is important to assess which behavioral patterns in women are hormonally mediated and which are dependent on the environment.     

Responses to olfactory stimuli in spotted hyenas (Crocuta crocuts): II. Discrimination of conspecific scent.

Scent marking in spotted hyenas (Crocuta crocuta) includes the deposition of anal sac secretions, or "paste," and presumably advertises territorial ownership. To test whether captive hyenas classify and discriminate individuals using odor cues in paste, the authors conducted behavioral discrimination bioassays and recorded hyena investigation of paste extracted from various conspecific donors. In Experiment 1, subjects directed most investigative behavior toward scents from unfamiliar hyenas and members of the opposite sex. In Experiment 2, male hyenas discriminated between concurrent presentations of paste from various unfamiliar females in similar reproductive states. Thus, pasted scent marks convey information about the sex, familiarity, and even identity of conspecifics. Aside from territory maintenance, scent marking may also communicate information about individual sexual status. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Food intake and the menstrual cycle: a retrospective analysis, with implications for appetite research

The biological regulation of appetite is currently an important topic in nutrition, since hyperphagia has been implicated as the prime cause of obesity. Cyclical fluctuations in food intake occur in women across the menstrual cycle, with a periovulatory nadir and a peak in the luteal phase. These alterations in food intake, in response to ovarian steroid hormone changes may be more than 2.5 MJ/day, with the mean reported changes shown in 19 separate studies of 1.0 MJ/day. Hormonal induced fluctuations in food intake could, therefore, contribute to energy imbalance and consequent weight gain. Further, in nutrition studies involving women subjects where the menstrual cycle phase is not controlled, hormonally induced changes in food selection and intake may mask the often considerably smaller changes in response to experimental variables in appetite research.     

Modulation of alloimmune response in vitro by an IgM-enriched immunoglobulin preparation (Pentaglobin)

OBJECTIVE: To describe endometrial wavelike activity, endometrial thickness, and texture in controlled ovarian hyperstimulation (COH) cycles. DESIGN: Prospective observational ultrasound study. SETTING: University hospital-based infertility clinic. PATIENT(S): Thirty-five COH cycles in 19 women with unexplained infertility. INTERVENTION(S): Transvaginal ultrasound examination was performed throughout COH cycles. Intrauterine insemination was performed after hCG administration. MAIN OUTCOME MEASURE(S): Endometrial wavelike activity, wave frequency, wave velocity, endometrial thickness, and endometrial texture. RESULT(S): Endometrial wavelike activity increased from menstruation to ovulation and decreased in the luteal phase. On day hCG+2, endometrial wave-like activity was observed in all cycles. Waves from cervix to fundus prevailed in the periovulatory phase. Endometrial wavelike activity was related significantly to endometrial thickness at the start of ovarian stimulation and in the luteal phase. Endometrial thickness increased throughout the cycle. Endometrial texture showed periovulatory a triple-line aspect. CONCLUSION(S): In COH cycles, endometrial wavelike activity is more pronounced than in spontaneous cycles. The number of follicles and endometrial wavelike activity were not correlated significantly. This is the first prospective study to provide longitudinal observational evidence that endometrial thickness increases throughout the COH cycle and that a triple line pattern develops.     

Expression of pS2, c-erbB-2, and cathepsin D during the menstrual cycle in human breast cancers

BACKGROUND: Many studies have addressed the effect of the timing of surgery for breast cancer relative to menstrual cycle phase, with conflicting results. Explanations for the possibility that survival could be altered by the appropriate timing of breast cancer surgery in humans remain speculative. METHODS: We examined the expression of three estrogen related proteins (c-erbB-2, cathepsin D, pS2) in the breast tumors from 69 premenopausal women sampled in different phases of the menstrual cycle. Data on S-phase fraction and hormone receptor expression were also analyzed. Immunohistochemical assays were used to measure the proteins of interest. S-phase fraction was determined by flow cytometry. Analyses were performed based on fraction of cells staining positive for the protein, density of stain, and a histoscore that combined both fraction of positive cells and density. RESULTS: We found no differences in c-erbB-2, cathepsin D, hormone receptor, or S-phase levels in tumors sampled in the follicular versus luteal phase, or perimenstrual versus periovulatory phase. The exception was pS2, which was expressed at greater levels during the luteal than during the follicular phase of the cycle (p < 0.01); but there was no difference in pS2 expression when the patients were classified as periovulatory versus perimenstrual. CONCLUSIONS: Our findings do not support a variation in c-erbB-2, cathepsin D, S-phase fraction, or receptor expression as an explanation for the differences in breast cancer prognosis when surgery is timed by menstrual cycle phase. The finding that pS2 (an indicator of hormone sensitivity, and possibly better prognosis) is expressed at higher levels in tumor samples during the luteal phase suggests that the biologic profile of breast tumors may vary with the menstrual cycle and that these variations deserve further study.     

Ovarian hormones and sexual behavior in Macaca nemestrina.

Determined the concentrations of the ovarian steroids, estradiol and progesterone, in the systemic plasma of 8 female pigtailed macaques and the correlations with sexual behavior and measurements of perineal swelling during the intermenstrual period. Estradiol peaked the day before maximum swelling of the perineum at the end of the 20.7-day follicular phase. Maximum titers of progesterone were recorded during the middle of the 15.1-day luteal phase. None of the female sexual behaviors changed significantly during the ovarian cycle, although intromission rate and frequency of ejaculation by 7 males were higher during the follicular phase. The pattern of ovarian hormone secretion and copulation throughout the menstrual cycle was more like that reported for women than for other mammalian species. (34 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Auditory event-related potentials, auditory digit span, and clinical symptoms in chronic schizophrenic men on neuroleptic medication

The Intervet Symposium 'Control and Regulation of Folliculogenesis' took place at the Annual Conference of the Society for the Study of Fertility in University College, Dublin in July, 1995. Five papers were presented, of which abstracts have been published. Discussion centred on monotocous species and principally addressed the question 'How is the ovulatory follicle selected and nurtured?' Many follicles start to develop simultaneously, but most become atretic. During the luteal phase of the cycle, several waves of follicles are initiated, and although one in each wave gains dominance, all ultimately atrophy. The dominant follicle in the wave produced towards the end of the luteal phase ovulates. The biochemical characteristics of dominant and subordinate follicles were contrasted, and the endocrine environment in which they waxed and waned was analysed. Interaction of autocrine and paracrine effectors, principally with FSH, LH and their receptors, ultimately determines follicular destiny.     

Cytogenetically unrelated clones in therapy-related myelodysplasia and acute myeloid leukemia: experience from the Copenhagen series updated to 180 consecutive cases

Among Callitrichids, scent secretions have been identified as carrying information regarding species, subspecies, gender, social status, individuality, hormonal status, and timing of ovulation. We propose that information regarding familiarity and reproductive status is also communicated. Seven male-female pairs of cotton-top tamarins (Saguinus oedipus oedipus) were presented with several drops of distilled water, a scent secretion from the female of that pair, scent secretions from unfamiliar, reproductively mature but noncycling females, and scent secretions from unfamiliar, reproductively cycling females. Behavioral responses from both males and females were recorded over a 10 min period. Differences in behavioral response for both males and females were significant across all four conditions. This indicates that cotton-top tamarins are capable of discriminating a familiar scent from an unfamiliar scent as well as the reproductive status of an unfamiliar female. The communication of this information may play an important role in regulating mate selection, reducing reproductive competition, and stabilizing transfers in and out of groups in the wild.     

Male-produced ultrasonic vocalizations and mating patterns in female rats.

Investigated the influence of male-produced 50-kHz vocalizations on mating behavior and choice of partner in estrous female rats. In Exp I, 17 estrous female Long-Evans rats were given a mating-choice test; 2 tethered males, one of which was devocalized, were used as incentives. Darting was directed toward the vocalizing male more frequently than toward the muted test partner, but ultrasonic vocalizations had no influence on visit rate or the amount of time that the females spent with each male. When 2 intact or 2 muted males were used as stimulus objects, the females' solicitation behavior was more evenly distributed between the 2 males. In Exp II, 16 estrous females were given a mating-choice test between 2 muted males; tape-recorded 50-kHz vocalizations or tape hiss was transmitted from a speaker located behind one of the males. Females spent equal amounts of time with the tethered males in both the vocalization and tape-hiss conditions; however, females directed more darting toward the males that were tethered in front of the speaker transmitting the ultrasonic vocalizations than toward the other muted tethered males. Findings demonstrate that the principal function of the male-produced ultrasounds is to facilitate and orient the solicitation behavior of the estrous female during mating. The vocalizations have little demonstrable effect in attracting the female to the immediate physical proximity of the male. (22 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Dominance fades with distance: An experiment on food competition in long-tailed macaques (Macaca fascicularis).

Dominant animals often can suppress the competitive behavior of subordinates by overt aggression or by their mere presence. This experiment on pairs of long-tailed macaque females explored whether this effect of dominant animals is influenced by interindividual distance. A dominant and a subordinate, but stronger, animal could compete for food by a tug-of-war on a bar. The animals were separated by 2 grids, spaced at either 30 or 100 cm. At 30 cm, 7 of 8 subordinate subjects either did not pull the bar or did not obtain a major share of the available food. In contrast, at 100 cm, all subordinate subjects obtained more food than the dominant. Thus, the dominant animal could suppress the competitive behavior of the subordinate only at the short interindividual distance, despite the fact that the dominant could not approach or attack even at the short distance and could be fully seen by the subordinate even at the large distance. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Social subordination stress, behavior, and central monoaminergic function in female cynomolgus monkeys

BACKGROUND: Social subordination in female cynomolgus monkeys is stressful and activates the hypothalamic-pituitary-adrenal axis. In a previous experiment behavioral depression was observed in a subset of subordinates. METHODS: In the experiment reported here behavioral and physiological indicators of stress were evaluated in dominant and subordinate female cynomolgus monkeys, and brain dopaminergic activity was assessed, as reflected in the prolactin response to haloperidol, a dopamine2 (D2) receptor antagonist. RESULTS: Subordinates were aggressed more, spent more time in fearful scanning of the social environment, spent less time as the recipients of the active affiliative behavior of being groomed, had more variable heart rates in response to a novel environment, and were hypercortisolemic compared to dominants. Prolactin responses to haloperidol challenge were lower in subordinates than dominants, an observation consistent with the hypothesis that subordinate females have decreased D2 receptor function. CONCLUSIONS: These observations suggest that social subordination is stressful and may alter brain dopaminergic function in primates. The neurophysiological characteristics of social subordinates may contribute to their susceptibility to depression.     

Effect of ovariectomy on the reproductive behavior of female ring doves (Streptopelia risoria).

Studied the behavior of 12 adult male and 12 adult female ring doves during the breeding period. The stages of female mating responsiveness were defined by characteristic behavioral events (e.g., approaching, nest soliciting, nest crouching, and nest building). In Exp. I, 24 females had their left (functional) ovary completely or partially removed or received sham operations. Sinistrally ovariectomized Ss lost their characteristic behavior patterns in response to courting: Their behaviors were regressed to that of the nonresponsive stage, where sleeping, perching, and other self-directed activities are the dominant behavior patterns. The characteristic responsive behavior patterns were recovered in some Ss as the right rudimentary ovary regenerated. In Exp. II with 25 female Ss, a 2-stage bilateral ovariectomy entirely abolished any chance of behavioral recovery. Results verify the role of ovarian hormones in female reproductive behavior in ring doves. (15 ref.) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Monitoring ovarian cycle and pregnancy in the giant anteater (Myrmecophaga tridactyla) by faecal progestagen and oestrogen analysis

Oestrogen and progestagen metabolites were measured in the faeces of five female giant anteaters (Myrmecophaga tridactyla), to characterise the oestrous cycle and pregnancy. Faecal samples were collected twice weekly for a minimum of 6 months, and immunoreactive progestagens and oestrogens were analysed using enzyme immunoassays (EIA). For progestagens, two antibodies that cross-reacted with 20alpha-hydroxy- or 20-oxo-progestagens were used. Both assays effectively monitored ovarian cyclicity; however, the concentrations obtained using the antibody for 20alpha-hydroxy-progestagens were higher, and the hormonal changes were more pronounced. Regular ovarian cycles were identified in three of the five females. Average (+/-SEM) length of the oestrous cycle (n=10) was 51.4+/-5.6 days. Peak concentrations of 20alpha-hydroxy-progestagens ranged from 80-660 ng/g of faeces and those of oestrogens from 20-100 ng/g. Hormone concentrations were measured during parts of two pregnancies and during four post-partum periods. The length of one gestation (from oestrous oestrogen peak until parturition) was 184 days. In the second half of gestation, progestagen concentration started to increase above luteal phase values; in the week before parturition it was approximately 20 times higher than those during the luteal phase. Concentrations of excreted oestrogens began to increase after two thirds of gestation and exceeded that of the follicular phase by approximately 2.5-fold in the week before parturition. Onset of ovarian cyclicity after parturition varied from 4-11 weeks. In conclusion, the measurement of faecal immunoreactive progestagens and oestrogens in the giant anteater indicated an ovarian cycle of approximately 7 weeks in length and provided potentially useful data for successful breeding management.     

Neonatal testosterone propionate treatment in the female gerbil: Morphological and behavioral effects.

The present experiment examined the effects of a single neonatal injection of 1 mg or 100 μg of testosterone propionate (TP) on the sexual behavior and morphology of the female Mongolian gerbil. Four groups were created: vehicle-treated males (VM), 1-mg TP-treated females (HTP), 100-μg TP-treated females (LTP), and vehicle-treated females (VF). In adulthood, tests of sexual behavior were carried out after gonadectomy and appropriate hormone replacement therapy. Results indicated that LTP, HTP, and VM animals were significantly less receptive than VF animals. In addition, VM animals displayed significantly more male sexual activity than HTP, LTP, or VF animals. Immediately after the final test for male sexual behavior, subjects were weighed, anogenital distances recorded, and scent glands measured (length and width). Results indicated that a significant degree of morphological masculinization had occurred in HTP subjects for all measures and for LTP subjects for scent gland width and anogenital distance. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

The activity of catechol-O-methyltransferase and monoamine oxidase in the uterine artery of pigs during the oestrous cycle

Activities of catechol-O-methyltransferase (COMT), total monoamine oxidase (MAO) and both types of MAO-A and MAO-B activities were examined in uterine artery on the 0-2nd, 13-14th and 16-18th days of the oestrous cycle in pigs. It was shown that activity of COMT was the lowest on the 0-2nd day, while on the 16-18th day of the oestrous cycle it increased by 52.4% (p < 0.05). Total activity of MAO was the highest at periovulatory phase, whereas on days 13-14 and 16-18 of oestrous cycle is was lower by 83.5% (p < 0.01) and 58.1% (p < 0.01) compared with its activity at periovulatory phase, and was higher on day 16-18 by 153.3% (p < 0.01) in relation to the luteal phase (13-14th day). MAO-A activity was 31.3% (p < 0.01) and MAO-B 62.5% (p < 0.05) of the total activity of MAO. Their activities were also highest at periovulatory phase, then decreased by 86.8-87.4% (p < 0.01) on 13-14th day and by 54.8-57.5% (p < 0.01) or 16-18th day of oestrous cycle. Activities of MAO-A and MAO-B were higher by 223.0-258.2% (p < 0.01) on 16-18th day in relation to the luteal phase (13-14th day). On that base we suppose that variations of COMT and MAO activities can significantly change the catecholamines content in the blood vessels of reproductive organs of pigs.     

Immunohistochemical distribution of follistatin in dominant and subordinate follicles and the corpus luteum of cattle

The study was done to quantitatively characterize the distribution of follistatin in ovarian follicles and corpora lutea at specific stages of development. Transrectal ultrasonography was used to monitor the growth of individually identified follicles from 2 days before ovulation until the day of ovariectomy on Day 3 of wave 1 (n = 8), Day 6 of wave 1 (n = 6), Day 1 of wave 2 (n = 6), or after onset of proestrus, at least 17 days postovulation (n = 7). Days of ovariectomy represent the growing, early-static, late-static, and regressing phases of the dominant follicle of wave 1, respectively. Subordinate (n = 24), preselection (n = 15), and preovulatory (n = 6) follicles and corpora lutea (n = 31) were also analyzed. Follistatin was localized using immunohistochemical labeling of paraffin sections, and relative amounts were quantitated using densitometric analysis. Follistatin was distributed in the perinuclear cytoplasm of granulosa and luteal cells but not in theca cells. Dominant follicles contained more (p < 0.05) follistatin than corresponding subordinate follicles. The amount of follistatin was maximal during the mid-growing phase of the dominant follicle and decreased thereafter (p < 0.05). Among the corpora lutea, the maximal amount was detected at mid-diestrus (Day 11 postovulation). Less than half of luteal cells displayed the stain for follistatin; positively stained luteal cells were located in close proximity to blood capillaries. Follistatin was not detectable in the corpus luteum during metestrus (Day 3 postovulation) or proestrus (Day >/= 17 postovulation). In summary, the degree of immunohistochemical expression of follistatin was phase specific for both follicles and corpora lutea. The most intense staining in follicles was associated with the period of functional dominance and in corpora lutea was seen during the phase of maximal development. Significant phase-related differences in follistatin expression provide rationale for the hypothesis that follistatin is involved in the final stages of follicle and luteal gland development in cattle.     

Intrasexual interactions among female golden hamsters (Mesocricetus auratus) over the estrous cycle.

Examined social interactions of cycling female golden hamsters paired with ovariectomized animals (N?=?96) in large enclosures. Interactions were primarily agonistic and intense over the 4-day estrous cycle, as indicated by frequent occurrences of chase and flight behavior. Dominant and subordinate social ranks were established in the majority of pairs (96%) tested, and even Ss in sexual heat were capable of attacking and dominating their rivals. Furthermore, cycling Ss exhibited significantly more aggressive acts than ovariectomized opponents 1 day prior to sexual receptivity. No differences in fighting patterns were found between Ss on the other 3 days of the estrous cycle. Additional analyses revealed clear differences in agonistic elements, including flank marking, between dominant and subordinate Ss, regardless of whether they were gonadectomized or intact. Analyses also show that dominant Ss frequently chased and bit opponents during encounters within their nesting area. Data are examined for implications on the adaptive organization of female hamster agonistic behavior and the neuroendocrine regulation of species-typical behavior over the estrous cycle. (23 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Effects of male social status on reproductive success and on behavior in mice (Mus musculus).

Differences in reproduction as well as in behavior in the presence of females were evaluated according to dominant and subordinate male rank in albino mice, in the temporary absence of each male's antagonist. Dominant males reproduced more successfully than subordinate males. Subordinate males were generally inactive, except for displacement activities, during the first 15 min they were exposed to female partners. These findings suggest that mechanisms other than male–male interference or mating order may be operating or influencing behavior and reproductive results. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Circadian changes in body temperature during the menstrual cycle of healthy adult females and patients suffering from premenstrual syndrome

The biological rhythm of females is closely related to the menstrual cycle, and this rhythm is believed to influence circadian changes in body temperature. This study investigated and compared the patterns of circadian changes in the body temperature of healthy adult females and patients suffering from premenstrual syndrome or major depression. Body temperature was measured both rectally and sublingually in healthy subjects, and only sublingually in the patients. During the luteal phase in healthy adult females, both the average and lowest nocturnal body temperatures increased, the amplitude of the circadian changes decreased, and the times of the lowest and highest temperatures within a 24-hour period were delayed by 2-3 h. In the patients, the amplitude decreased during disease periods, especially in the follicular phase, whereas in the luteal phase, circadian changes showed great variation each day, although the decrease in amplitude was not as remarkable. The results show that (i) the biological rhythm of females is intrinsically unstable in the luteal phase, although this rhythm is stable in the follicular phase; and, (ii) symptoms were often aggravated with the decreases in amplitude experienced in the luteal phase.     

The onset of the ovarian cycle following calving

The onset of ovarian activity post partum was investigated by measuring progesterone concentrations in milk samples, in two dairy herds consisting of 118 cows with an average milk yield of 8340 kg FCM. Samples were taken three times a week till 50 days post partum. In 17 cows (14.4%) anoestrus occurred. The daily milk yield in this group was 2.65 kg FCM higher than the average yield in the group returning to oestrus before day 50 post partum. In cows returning to oestrus within 50 days post partum the first rise in progesterone was detected on average 27.6 days after calving. In first calvers (31.4 +/- 10.2) and in multiparous cows in the winter period (26.9 +/- 9.4) the onset of ovarian activity was delayed compared to start of ovarian activity in the summer period. In the first cycle only 28% of the cows had a normal luteal phase (12-17 days), 36% of the cows had a shortened luteal phase (less than 6 days), and 24% of the cows had a short luteal phase (6-11 days). In 12% of the cows the luteal phase was longer than 17 days. In the second cycle 56% of the cows had a normal luteal phase while 17% had a shortened luteal phase, and 17% had a short luteal phase. Pregnancy rates after first insemination in cows with a short dioestrus (10-25 days) were higher than in cows with a prolonged dioestrus (26-50 days). On the basis of these result it might be expected that postponing the first insemination until the second or even the third cycle in high-yielding cows will have only a marginal effect on the number of open days and a large effect on the number of inseminations per pregnancy.