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应用响应面法优化植物乳杆菌培养基配方以及利用中和法与指数流加法优化植物乳杆菌高密度培养的发酵条件。在单因素试验基础上,进一步采用SAS软件进行中心组合设计和响应面法优化发酵培养基。优化后的培养基配方为:葡萄糖质量分数5.43%、蛋白胨质量分数0.98%、K2HPO4质量分数0.59%。利用15L全自动发酵罐,在接种量3%、pH6.5、培养温度35℃的最佳条件下,采用氨水中和发酵培养基和指数流加碳、氮源,最终发酵液中植物乳杆菌菌体浓度达到9.3×109CFU/mL。 相似文献
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以胞外多糖含量和菌丝体生物量为测定指标对鸡腿蘑深层发酵培养基的优化进行了研究。结果表明:鸡腿蘑深层发酵优化培养基的配方为玉米淀粉4.0%,黄豆饼粉0.2%,蔗糖3.0%,KH2PO40.1%,MgSO40.1%,CaSO40.05%,玉米浆0.5%。 相似文献
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本实验以Streptomyces avermitilis为实验菌株,通过均匀设计方法优化了柞蚕生产用avermectins发酵培养基.结果表明:以玉米淀粉9%、黄豆饼粉0.5%、花生饼粉0.7%、酵母粉0.8%、酵母膏0.1%、玉米浆0.3%、CoCl2*6H2O 0.003%、(NH4)2SO4 0.02%的培养基配方可使avermectins发酵产量最高. 相似文献
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为将实验室诱变选育出的高产甲醇蛋白毕赤酵母菌高效表达木聚糖酶,在100 L发酵罐中,对影响木聚糖酶发酵水平的培养基组分及其质量浓度等因素进行考查,初步确定培养基配方,并设计正交试验优化发酵培养基.结果表明:最优发酵培养基配方为H3PO4质量浓度25 g/L、K2HPO4质量浓度0.5 g/L、NH4 Cl质量浓度0.3... 相似文献
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运用遗传算法,利用莫格假丝酵母由木糖生产木糖醇的发酵培养基进行优化,用40个实验样本完成了6种培养基成分、50个浓度水平的优化任务.实验结果表明利用遗传算法可优化培养基成分含量,取得更好的发酵效果.按照优化后的培养基组成,由50g/L木糖获得了29.7g/L木糖醇,理论转化率为65.1%,比优化前提高了3.5%. 相似文献
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以玉米芯为原料,采用WO3/ZrO2固体酸进行预处理,然后发酵制备木糖醇。探讨了预处理温度、预处理时间、固体酸用量及液固比对木糖醇得率的影响。采用响应面法建立二次回归模型,并对预处理工艺进行了优化。研究结果表明固体酸预处理能有效的促进玉米芯降解,提高木糖醇的得率。当预处理温度为110.76℃、预处理时间为60.71 min、固体酸用量为4.14%时,木糖醇的得率比相同条件下未进行固体酸预处理的试样提高了25.21%。 相似文献
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Increase of xylitol productivity by cell-recycle fermentation of Candida tropicalis using submerged membrane bioreactor 总被引:5,自引:0,他引:5
Candida tropicalis, an osmophilic strain isolated from honeycomb, produced xylitol at a maximal volumetric productivity of 3.5 g l(-1) h(-1) from an initial xylose concentration of 200 g l(-1). Even at a very high xylose concentration, e.g., 350 g l(-1), this strain produced xylitol at a moderate rate of 2.07 g l(-1) h(-1). In a fed-batch fermentation of xylose and glucose, 260 g l(-1) xylose was added, and the xylitol production was 234 g l(-1) for 48 h, corresponding to a rate of 4.88 g l(-1) h(-1). To increase xylitol productivity, cells were recycled in a submerged membrane bioreactor with suction pressure and air sparging. For each recycle round in cell-recycle fermentation, the average concentration of xylitol produced, fermentation time, volumetric productivity, and product yield were 180 g l(-1), 19.5 h, 8.5 g l(-1) h(-1), and 85%, respectively. When cell-recycle fermentation was started with the cell mass concentrated twofold after batch fermentation and performed for 10 recycle rounds, we achieved a very high productivity of 12 g l(-1) h(-1). The productivity and total amount of xylitol in cell-recycle fermentation were 3.4- and 11.0-fold higher than those in batch fermentation, respectively. 相似文献