共查询到20条相似文献，搜索用时 15 毫秒
1.
Yukun Duan Min Wu Dehong Hu Yutong Pan Fang Hu Xingang Liu Nitish Thakor Wai Hoe Ng Xin Liu Zonghai Sheng Hairong Zheng Bin Liu 《Advanced functional materials》2020,30(38)
Brain tumor targeted delivery of diagnostic contrast agents has been an elusive goal due to the presence of the blood brain barrier (BBB) and the complex brain tumor microenvironment. Herein, an ingenious design of nanoscale contrast agents coated by bioorthogonally labeled brain tumor cell membrane for targeted diagnosis of glioblastoma through multiple complementary imaging modalities is presented. Taking advantage of bioorthogonal click reactions, an endothelial integrin receptor‐targeting peptide cRGD is decorated onto the nanocomposite surface to act in synergy with brain tumor cell membrane to offer BBB‐penetrating and homotypic targeting effect in the brain tumor microenvironment. Cellular and animal experimental results validate the superior targeting outcomes achieved by cRGD‐labeled brain tumor cell membrane coating. This study offers an example of a surface modified cell membrane as a potential theranostic strategy to overcome the delivery barriers in brain tumors. 相似文献
2.
Ruodan Lan Jianxun Lv Duyang Gao Dehong Hu Chunchen Liu Jie Jia Hairong Zheng Chengbo Liu Ping Zhao Zonghai Sheng 《Advanced functional materials》2023,33(29):2300342
Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by joint inflammation. Macrophages accelerate the pathological process of RA through the continuous cell accumulation and secretion of cytokines. Therefore, multiscale visualization of macrophages is of great significance for the early diagnosis of RA. In this work, folate receptor-targeted Cptnc-4F nanoprobes, named FA-CF-NPs, are developed for dual-model molecular imaging of macrophages in RA mouse models. FA-CF-NPs exhibit complementary imaging advantages with high fluorescence sensitivity and resolution in the second near-infrared window (NIR-II) and promising photoacoustic (PA) imaging contrast with deep tissue penetration. The high affinity of the folate ligand on the surface of FA-CF-NPs to macrophages with overexpressed folate receptors ensures the specific targeting function of the nanoprobes for imaging in vivo. FA-CF-NPs are employed to achieve NIR-II fluorescence imaging to accurately localize macrophages in the joint, obtaining a high signal-to-background ratio (SBR) of 15.5. Meanwhile, PA imaging is also performed to observe macrophage distribution in the longitudinal section of the whole joint. Moreover, macrophage infiltration is detected in the joint of RA mice with the absence of RA pathologies such as synovitis and cartilage loss. The results provide an opportunity for in vivo macrophage tracking and early diagnosis of RA. 相似文献
3.
Yukun Duan Dehong Hu Bing Guo Qi Shi Min Wu Shidang Xu Kenry Xin Liu Jianwen Jiang Zonghai Sheng Hairong Zheng Bin Liu 《Advanced functional materials》2020,30(1)
The performance of current multimodal imaging contrast agents is often constrained by the tunability of nanomaterial structural design. Herein, the influence of nanostructure on the overall imaging performance of a composite nanomaterial for multimodal imaging of brain tumors is studied. Newly designed near‐infrared molecules (TC1) are encapsulated into nanocomposites with ultrasmall iron oxide nanoparticles (UIONPs), forming stable nanoagents for multimodal imaging and photothermal therapy (PTT). Through a modified nanoprecipitation method, the synthesis of nanocomposites denoted as HALF is realized, in which UIONPs are restricted to half of the nanosphere. Such a unique nanostructure that physically separates TC1 and UIONPs is found with capabilities of mitigating fluorescence quenching, preserving the good performance of photoacoustic imaging, and enhancing the magnetic resonance imaging signals. Decorated with a peptide ligand cRGD for better brain tumor targeting, HALF‐cRGD is evaluated both in vitro and in vivo as imaging contrast agents and photothermal therapeutic agents. The good imaging performance and PTT effect of HALF‐cRGD in mice models indicate that the rational design and control of nanostructures could optimize multimodal imaging performance using the same components. 相似文献
4.
Xulei Qin Haodong Chen Huaxiao Yang Haodi Wu Xin Zhao Huiyuan Wang Tony Chour Evgenios Neofytou Dan Ding Heike Daldrup‐Link Sarah C. Heilshorn Kai Li Joseph C. Wu 《Advanced functional materials》2018,28(1)
Human embryonic stem cell‐derived cardiomyocytes (hESC‐CMs) have become promising tools to repair injured hearts. To achieve optimal outcomes, advanced molecular imaging methods are essential to accurately track these transplanted cells in the heart. In this study, it is demonstrated for the first time that a class of photoacoustic nanoparticles (PANPs) incorporating semiconducting polymers (SPs) as contrast agents can be used in the photoacoustic imaging (PAI) of transplanted hESC‐CMs in living mouse hearts. This is achieved by virtue of two benefits of PANPs. First, strong photoacoustic (PA) signals and specific spectral features of SPs allow PAI to sensitively detect and distinguish a small number of PANP‐labeled cells (2000) from background tissues. Second, the PANPs show a high efficiency for hESC‐CM labeling without adverse effects on cell structure, function, and gene expression. Assisted by ultrasound imaging, the delivery and engraftment of hESC‐CMs in living mouse hearts can be assessed by PANP‐based PAI with high spatial resolution (≈100 µm). In summary, this study explores and validates a novel application of SPs as a PA contrast agent to track labeled cells with high sensitivity and accuracy in vivo, highlighting the advantages of integrating PAI and PANPs to advance cardiac regenerative therapies. 相似文献
5.
频率域光声成像是指使用连续波激光器输出激光光源,利用该调制后的激光信号辐射组织,在频率域对激励的光声信号进行处理并成像的方式。首先阐述光声成像和频率域光声成像的原理、发展以及研究现状,然后介绍频率域光声成像的具体实验方法和基本重建算法。为了弥补时域光声成像成本高、伤害性大、便携性差等缺点,提出了频率域光声成像的两种成像方式,给出了完整的实验系统结构图,展示其相对于传统时域光声成像不同的研究特点与方向。最后对频率域光声成像进行展望,为光声成像在国内的研究和发展提供一定的借鉴和引导。 相似文献
6.
Chao Yin Xiaozhen Li Yi Wang Yuying Liang Shan Zhou Pengchao Zhao Chun-Sing Lee Quli Fan Wei Huang 《Advanced functional materials》2021,31(37):2104650
Owing to the unique advantages of photoacoustic imaging (PAI) and photothermal therapy (PTT) conducted over the near-infrared-II (NIR-II) window, the development of high-efficiency optical agents with NIR-II light responsiveness is of great significance. Despite the diversity of optical agents developed for NIR-II PAI and PTT, most of them are based on inorganic nanomaterials and small molecular dyes, whose biosafety and photostability need to be further assessed, respectively. Organic semiconducting macromolecular dyes (OSMDs) featuring a large semiconducting backbone are becoming alternative candidates for NIR-II PAI and PTT owing to their reliable biocompatibility, durable photostability, and ideal photothermal conversion capability. This paper reviews the current progress of OSMD-based PAI and PTT in the NIR-II optical window. The three main types of OSMDs with different skeleton architectures are introduced, and their applications for NIR-II PAI (tumor imaging, stem cell tracking, and vasculature imaging) and PTT (tumor ablation) are described. Viable strategies for further improving the NIR-II PAI performance of OSMDs are discussed. Finally, some major issues faced by OSMDs in NIR-II PAI and PTT are raised, and the future development directions of OSMDs are analyzed. 相似文献
7.
8.
为了分析在不同激发波长下不同组织/目标光声信号的时域与频域特点和差异,根据光声信号产生的基本原理,采用光声信号时域和频域分析方法,设计了石墨仿体和离体组织等不同样品的多光谱光声实验。结果表明,样品的光声信号在时域与频域所展示的性能有很大的不同,不同样品的光声信号的声谱是不同的,且在不同激发波长下的声谱的峰值所对应的频率均相同,因而可以用于组织特性的描述、组分识别等用途。此研究有助于利用光声成像实现组织识别,并为进一步利用频谱分析方法研究多光谱光声成像奠定了基础。 相似文献
9.
Kai Li Dan Ding Da Huo Kan‐Yi Pu Ngo Nguyen Phuong Thao Yong Hu Zhi Li Bin Liu 《Advanced functional materials》2012,22(15):3107-3115
A facile strategy is developed to synthesize dual‐modal fluorescent‐magnetic nanoparticles (NPs) with surface folic acid by co‐encapsulation of a far‐red/near‐infrared (FR/NIR)‐emissive conjugated polymer (PFVBT) and lipid‐coated iron oxides (IOs) into a mixture of poly(lactic‐co‐glycolic‐acid)‐poly(ethylene glycol)‐folate (PLGA‐PEG‐FOL) and PLGA. The obtained NPs exhibit superparamagnetic properties and high fluorescence, which indicates that the lipid coated on IOs is effective at separating the conjugated polymer from IOs to minimize fluorescence quenching. These NPs are spherical in shape with an average diameter of ≈180 nm in water, as determined by laser light scattering. In vitro studies reveal that these dual‐modal NPs can serve as an effective fluorescent probe to achieve targeted imaging of MCF‐7 breast cancer cells without obvious cytotoxicity. In vivo fluorescence and magnetic resonance imaging results suggest that the NPs are able to preferentially accumulate in tumor tissues to allow dual‐modal detection of tumors in a living body. This demonstrates the potential of conjugated polymer based dual‐modal nanoprobes for versatile in vitro and in vivo applications in future. 相似文献
10.
研究了光声信号声速失配时所起的声折射对光声成像的影响,提出了利用与组织声速匹配的耦合液进行光声成像,并配置了几种适合于组织声速的超声耦合液.实验结果表明,进行声速匹配后重建出的图像对比度有明显地提高,背景伪迹减少,分辨率由0.50 mm提高到0.15 mm,重建图像与实物十分吻合. 相似文献
11.
Xiaoju Men Fei Wang Haobin Chen Yubin Liu Xiaoxiao Men Ye Yuan Zhe Zhang Duyang Gao Changfeng Wu Zhen Yuan 《Advanced functional materials》2020,30(24)
Phototheranostic agents in the second near‐infrared (NIR‐II) window (1000–1700 nm) are emerging as a promising theranostic platform for precision medicine due to enhanced penetration depth and minimized tissue exposure. The development of metabolizable NIR‐II nanoagents for imaging‐guided therapy are essential for noninvasive disease diagnosis and precise ablation of tumors. Herein, metabolizable highly absorbing NIR‐II conjugated polymer dots (Pdots) are reported for the first time for photoacoustic imaging guided photothermal therapy (PTT). The unique design of low‐bandgap D‐A π‐conjugated polymer (DPP‐BTzTD) together with modified nanoreprecipitation conditions allows to fabricate NIR‐II absorbing Pdots with ultrasmall (4 nm) particle size. Extensive experimental tests demonstrate that the constructed Pdots exhibit good biocompatibility, excellent photostability, bright photoacoustic signals, and high photothermal conversion efficiency (53%). In addition, upon tail‐vein intravenous injection of tumor‐bearing mice, Pdots also show high‐efficient tumor ablation capability with rapid excretion from the body. In particular, both in vitro and in vivo assays indicate that the Pdots possess remarkable PTT performance under irradiation with a 1064 nm laser with 0.5 W cm?2, which is much lower than its maximum permissible exposure limit of 1 W cm?2. This pilot study thus paves a novel avenue for the development of organic semiconducting nanoagents for future clinical translation. 相似文献
12.
Qi Wang Yeneng Dai Jingzeng Xu Jie Cai Xinrui Niu Lei Zhang Runfeng Chen Qingming Shen Wei Huang Quli Fan 《Advanced functional materials》2019,29(31)
Development of single near‐infrared (NIR) laser triggered phototheranostics for multimodal imaging guided combination therapy is highly desirable but is still a big challenge. Herein, a novel small‐molecule dye DPP‐BT is designed and synthesized, which shows strong absorption in the first NIR window (NIR‐I) and fluorescence emission in the second NIR region (NIR‐II). Such a dye not only acts as a dual‐modal contrast agent for NIR‐II fluorescence and photoacoustic (PA) imaging, but also serves as a combined therapeutic agent for photothermal therapy (PTT) and photodynamic therapy (PDT). The single NIR laser triggered all‐in‐one phototheranostic nanoparticles are constructed by encapsulating the dye DPP‐BT, chemotherapy drug DOX, and natural phase‐change materials with a folic acid functionalized amphiphile. Notably, under NIR laser irradiation, DOX can effectively release from such nanoparticles via NIR‐induced hyperthermia of DPP‐BT. By intravenous injection of such nanoparticles into Hela tumor‐bearing mice, the tumor size and location can be accurately observed via NIR‐II fluorescence/PA dual‐modal imaging. From in vitro and in vivo therapy results, such nanoparticles simultaneously present remarkable antitumor efficacy by PTT/PDT/chemo combination therapy, which is triggered by a single NIR laser. Overall, this work provides an innovative strategy to design and construct all‐in‐one nanoplatforms for clinical phototheranostics. 相似文献
13.
生物组织的光声成像技术及其在生物医学中的应用 总被引：1，自引：0，他引：1
简要介绍了光声成像技术的基本原理,采集系统和成像算法.重点阐述了光声成像技术在肿瘤的早期检测和疗效监测,脑成像和脑功能监测以及临床血管监测等生物医学领域的应用.对光声成像技术应用前景进行了展望. 相似文献
14.
为了实现光声信号的快速采集和图像重建,采用基于Labview软件平台的光声信号采集和成像系统进行光声信号采集,并调用Matlab程序对采集到的数据进行处理,最后利用滤波反投影重建算法,实现了对模拟组织样品的光声层析成像。成像系统的分辨率为0.15mm,重建图像与实物十分吻合。实验表明,该系统具有快速、方便、直观等特点,有望发展成为一种低成本的实用的临床诊断仪器。 相似文献
15.
The size and shape of coordination polymers (CPs) are often tuned by external factors including reaction temperature, reaction time, precursor ratio, auxiliary ligand, and surfactant. Here, a self‐limiting growth of uniform nanoscale CPs (NCPs) spheres with Gd3+ and Ru[4,4′‐(COOH)2 bipyridyl(bpy)]32+ ( LRu ) as precursors is reported. Sexadentate LRu and nine‐coordinating Gd3+ play key roles in the formation of the NCPs via a simple and robust self‐limiting procedure. Therefore, the formation of NCP spheres is almost unaffected in the reaction temperature of 100–160 °C for 1–6 h. Moreover, no auxiliary ligand or surfactant is required, whereas high yield and simple procedure are obtained. The red fluorescence of LRu and high longitudinal relaxivity of Gd3+ remain in the NCPs, which are therefore examined as fluorescence‐magnetic resonance (MR) dual‐modality imaging probes. The structural merits of the NCPs enable high MR contrast efficiency. Red emission avoids the auto‐fluorescence and light scattering from tissues and realizes low‐background imaging. The low toxicity and background, and high imaging efficiency of the NCPs are confirmed using HepG2 cells, zebrafish, and tumor‐bearing mice as models. 相似文献
16.
17.
该文制备了巯基聚乙二醇（PEG）修饰的金纳米棒,该纳米材料在近红外区具有良好的光吸收特性,具备作为优良光声造影剂的潜质。该文通过透射电子显微镜（TEM）、紫外 可见（UV VIS）吸收光谱等测量手段对金纳米棒进行了形貌、结构、基本光学性能及光声成像效果等表征。实验结果表明,随着材料浓度的增加,体外光声信号的响应近似呈线性增长;经由PEG修饰,金纳米棒的生物相容性得到提高;PEG修饰后的金纳米棒对小鼠大脑皮层血管的成像效果得到提升。结果表明,PEG修饰的金纳米棒材料,在光声成像造影领域具有巨大的应用前景。 相似文献
18.
Dong Cui Pengcheng Li Xu Zhen Jingchao Li Yuyan Jiang Aixi Yu Xiang Hu Kanyi Pu 《Advanced functional materials》2019,29(38)
Photoacoustic (PA) agents with biomarker‐activated signals are developed to enhance the signal‐to‐background ratios (SBRs) for in vivo imaging; however, their SBRs still heavily rely on the concentration difference of biomarkers between diseased and normal tissues. By contrast, external stimuli can provide a remote way to noninvasively control the signal generation from the PA agents and in turn enhance SBR, which are less exploited. This study reports the development of thermoresponsive semiconducting polymer brush with poly(N,N‐dimethylacrylamide)‐r‐(hydroxypropyl acrylate) (PDMA‐r‐HPA) grafts for contrast‐enhanced in vivo imaging. Such a polymer is amphiphilic and can self‐assemble into the nanoparticle (termed as SPNph1) in an aqueous medium, and has lower critical solution temperatures (LCSTs) at 48 °C. Thus, SPNph1 not only has higher photothermal conversion efficiency than the control polymer without PDMA‐r‐HPA grafts, but also can undergo phase separation to form large nanoparticles, leading to enhanced PA signals above LCST. The thermoresponsive PA property of SPNph1 enables in situ remote manipulation of PA signals by photoirradiation to further enhance the tumor SBR. Thus, this study introduces a new generation of organic PA agents with thermoresponsive signal for high‐contrast in vivo imaging. 相似文献
19.
多种光声成像方法研究 总被引：1，自引：0，他引：1
阐述了光声成像的基本原理,综述了现有的几种光声成像方法、原理及成果。介绍了国内外多个研究小组在光声成像领域所取得的一些成果,主要包括:用超声探测器记录光声信号的滤波反投影算法光声成像;超声探测器结合声透镜的傅里叶方法;用探测光做载波调制光声信号,将光声信号转化为光信号,经光学系统解调,将样品像显示在CCD摄像机上的实时光声成像等。对比了几种光声成像方法,并提出了一些光声成像的新思路。 相似文献
20.
光声成像技术在生物医学中的研究进展 总被引：1，自引：0，他引：1
简要介绍了生物医学中的光声成像技术机理,总结报道了国内外几种典型的光声成像方法和光声图像重建算法的发展历程及其最新进展,指出该技术是一种很有应用前景的医学检测方法。 相似文献