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1.
Pilar Luna Manuela Juárez Miguel Angel de la Fuente 《European Journal of Lipid Science and Technology》2007,109(12):1160-1166
The fatty acid composition of 39 mature human milk samples from four Spanish women collected between 2 and 18 weeks during lactation was studied by gas chromatography. The conjugated linoleic acid (CLA) isomer profile was also determined by silver‐ion HPLC (Ag+‐HPLC) with three columns in series. The major fatty acid fraction in milk lipids throughout lactation was represented by the monounsaturated fatty acids, with oleic acid being the predominant compound (36–49% of total fatty acids). The saturated fatty acid fraction represented more than 35% of the total fatty acids, and polyunsaturated fatty acids ranged on average between 10 and 13%. Mean values of total CLA varied from 0.12 to 0.15% of total fatty acids. The complex mixture of CLA isomers was separated by Ag+‐HPLC. Rumenic acid (RA, cis‐9 trans‐11 C18:2) was the major isomer, representing more than 60% of total CLA. Trans‐9 trans‐11 and 7‐9 (cis‐trans + trans‐cis) C18:2 were the main CLA isomers after RA. Very small amounts of 8‐10 and 10‐12 C18:2 (cis‐trans + trans‐cis) isomers were detected, as were different proportions of cis‐11 trans‐13 and trans‐11 cis‐13 C18:2. Although most of the isomers were present in all samples, their concentrations varied considerably. 相似文献
2.
Maternal dietary conjugated linoleic acid alters hepatic triacylglycerol and tissue fatty acids in hatched chicks 总被引:1,自引:0,他引:1
The effects of feeding CLA to hens on newly hatched chick hepatic and carcass lipid content, liver TAG accumulation, and FA
incorporation in chick tissues such as liver, heart, brain, and adipose were studied. These tissues were selected owing to
their respective roles in lipid assimilation (liver), as a major oxidation site (heart), as a site enriched with long-chain
polyunsaturates for function (brain), and as a storage depot (adipose). Eggs with no, low, or high levels of CLA were produced
by feeding hens a corn-soybean meal-basal diet containing 3% (w/w) corn oil (Control), 2.5% corn oil +0.5% CLA oil (CLA1),
or 2% corn oil +1.0% CLA oil (CLA2). The egg yolk content of total CLA was 0.0, 1.0, and 2.6% for Control, CLA1, and CLA2,
respectively (P<0.05). Maternal dietary CLA resulted in a decrease in chick carcass total fat (P<0.05). Liver tissue of CLA2 chicks had the lowest fat content (P<0.05). The liver TAG content was 8.2, 5.8, and 5.1 mg/g for Control, CLA1, and CLA2 chicks, respectively (P<0.05). The chicks hatched from CLA1 and CLA2 incorporated higher levels of cis-9,trans-11 CLA in the liver, plasma, adipose, and brain than Control (P<0.05). The content of 18∶0 was higher in the liver, plasma adipose, and brain of CLA1 and CLA2 than Control (P<0.05), but no difference was observed in the 18∶0 content of heart tissue. A significant reduction in 18∶1 was observed in
the liver, plasma, adipose, heart, and brain of CLA1 and CLA2 chicks (P<0.05). DHA (22∶6n−3) was reduced in the heart and brain of CLA1 and CLA2 chicks (P<0.05). No difference was observed in carcass weight, dry matter, or ash content of chicks (P>0.05). The hatchabilities of fertile eggs were 78, 34, and 38% for Control, CLA1, and CLA2, respectively (P<0.05). The early dead chicks were higher in CLA1 and CLA2 than Control (18 and 32% compared with 9% for Control), and alive
but not hatched chicks were 15 and 19% for CLA1 and CLA2, compared with 8% for Control (P<0.05). Maternal supplementation with CLA leads to a reduction in hatchability, liver TAG, and carcass total fat in newly
hatched chicks. 相似文献
3.
Lipase-catalyzed fractionation of conjugated linoleic acid isomers 总被引:14,自引:0,他引:14
Haas MJ Kramer JK McNeill G Scott K Foglia TA Sehat N Fritsche J Mossoba MM Yurawecz MP 《Lipids》1999,34(9):979-987
The abilities of lipases produced by the fungus Geotrichum candidum to selectively fractionate mixtures of conjugated linoleic acid (CLA) isomers during esterification of mixed CLA free fatty
acids and during hydrolysis of mixed CLA methyl esters were examined. The enzymes were highly selective for cis-9,trans-11–18∶2. A commercial CLA methyl ester preparation, containing at least 12 species representing four positional CLA isomers,
was incubated in aqueous solution with either a commercial G. candidum lipase preparation (Amano GC-4) or lipase produced from a cloned high-selectivity G. candidum lipase B gene. In both instances selective hydrolysis of the cis-9,trans-11–18∶2 methyl ester occurred, with negligible hydrolysis of other CLA isomers. The content of cis-9,trans-11–18∶2 in the resulting free fatty acid fraction was between 94 (lipase B reaction) and 77% (GC-4 reaction). The commercial
CLA mixture contained only trace amounts of trans-9,cis-11–18∶2, and there was no evidence that this isomer was hydrolyzed by the enzyme. Analogous results were obtained with these
enzymes in the esterification in organic solvent of a commercial preparation of CLA free fatty acids containing at least 12
CLA isomers. In this case, G. candidum lipase B generated a methyl ester fraction that contained >98% cis-9,trans-11–18∶2. Geotrichum candidum lipases B and GC-4 also demonstrated high selectivity in the esterification of CLA with ethanol, generating ethyl ester fractions
containing 96 and 80%, respectively, of the cis-9,trans-11 isomer. In a second set of experiments, CLA synthesized from pure linoleic acid, composed essentially of two isomers,
cis-9,trans-11 and trans-10,cis-12, was utilized. This was subjected to esterification with octanol in an aqueous reaction system using Amano GC-4 lipase
as catalyst. The resulting ester fraction contained up to 97% of the cis-9,trans-11 isomer. After adjustment of the reaction conditions, a concentration of 85% trans-10,cis-12–18∶2 could be obtained in the unreacted free fatty acid fraction. These lipase-catalyzed reactions provide a means for
the preparative-scale production of high-purity cis-9,trans-11–18∶2, and a corresponding CLA fraction depleted of this isomer. 相似文献
4.
A representative number of different milk fats based on a wide range of feeding and lactation conditions as well as 123 German margarines, shortenings, cooking and dietetic fats were analyzed for a variety of trans-C18:2 isomers (exhibiting at least one trans double bond) by means of gas chromatography on a 100m Sil 88 capillary column. In milk fats contents of trans Δ9,trans Δ12,cis Δ9,trans Δ13 (+ trans Δ8,cis Δ12),trans Δ8,cis Δ13,cis Δ9,trans Δ12,trans Δ9,cis Δ12 and trans Δ11,cis Δ15 amounted to 0.09%, 0.11%, 0.11%, 0.10%, 0.07% and 0.33% on average and the content of total trans-C18:2 isomers (without cis Δ9, trans Δ11) was 0.99% in the mean. The content of conjugated linoleic acid cis Δ9,trans Δ11 amounted to 0.81% on average in 238 milk fats. In margarine among others the isomers trans Δ9,trans Δ12,cis Δ9,trans Δ13 (+ trans Δ8,cis Δ12), cis Δ9,trans Δ12 and trans Δ9,cis Δ12 were determined and quantified to 0.03%, 0.04%, 0.29% and 0.23% on average. The mean total content of trans-C18:2 isomers in margarines was 0.61%. Moreover, for all trans-C18:2 isomers the frequency distributions as well as the correlation coefficients towards the trans-C18:1 isomers trans Δ6 to trans Δ16 were derived. 相似文献
5.
The amounts of Δ9,Δ11-conjugated linoleic acid (CLA) isomers were determined in loin-associated fat samples of bulls (n=6) and steers (n=7) by capillary gas chromatography of fatty acid methyl ester (FAME) derivatives. The main CLA-isomer—18:2 c9,t11—provided approximately 0.76 ± 0.15% and 0.86 ± 0.15% of total FAME in bulls and steers, respectively. No differences (P>0.05) were observed between the CLA isomer distribution of bulls (t9,c11, 0.026 ± 0.014%; c9,c11, 0.015 ± 0.008%; and t9,t11, 0.029 ± 0.003%) and steers (t9,c11, 0.027 ± 0.014%; c9,c11, 0.015 ± 0.005%; and t9,t11, 0.030 ± 0.007%). 相似文献
6.
Frdric Destaillats Paul Angers 《European Journal of Lipid Science and Technology》2005,107(3):167-172
Chemical pathways responsible of the conjugation of linoleic acid during heat treatments such as refining (deodorization), frying or cooking processes have been investigated. For this purpose, methyl linoleate was submitted to oxidative and non‐oxidative thermal conditions. The resulting degradation products were mainly composed of geometrical and conjugated fatty acid isomers. Oxidative conditions were obtained using tert‐butyl hydroperoxide under inert atmosphere, and air. The obtained results from both thermal oxidative conditions were compared to non‐oxidative thermal treatment. Higher levels of conjugated linoleic acid were found when linoleate was heated under oxidative conditions. Two distinct mechanisms responsible for the formation of CLA isomers are proposed and discussed. Evidence of formation of 9,11‐C18:2 and 10,12‐C18:2 acids from 9,12‐C18:2 by a free‐radical chain reaction is provided. The first step consists in the formation of a free radical by abstraction of an active bis‐allylic hydrogen. By delocalization of the initial free radical, two allylic free radicals were stabilized and converted into the corresponding CLA isomers via the abstraction of a hydrogen radical from other linoleic acid or oxygenated species. Kinetic observations confirmed the significance of the bimolecular mechanism. Moreover, the proposed mechanism is supported by several pieces of information from the literature on peroxidation of linoleic acid. Under pure thermal conditions and/or for diluted samples, a second pathway to the formation of CLA from heat‐treated linoleic acid is proposed via an intramolecular rearrangement of the pentadienyl structure. This thermal [1,3]‐sigmatropic rearrangement results in a mixture of 9,11 and 10,12 CLA isomers. The formed cis/trans CLA isomers were readily rearranged by a [1,5]‐sigmatropic shift to yield trans‐8,cis‐10 and cis‐11,trans‐13 CLA isomers, respectively. 相似文献
7.
Jan Fritsche Sonja Fritsche M. B. Solomon M. M. Mossoba M. P. Yurawecz K. Morehouse Y. Ku 《European Journal of Lipid Science and Technology》2000,102(11):667-672
The amounts of 14 conjugated linoleic acid (CLA) isomers (t12t14, t11t13, t10t12, t9t11, t8t10, t7t9, t6t8; 12,14 c/t, t11c13, c11t13, t10c12, 9,11 c/t, t8c10, t7c9‐18:2) in 20 beef samples were determined by triple‐column silver‐ion high‐performance liquid chromatography (Ag+‐HPLC). Quantitation was performed using an external CLA reference standard consisting of cis9,trans11‐18:2,trans9,trans11‐18:2 and cis9,cis11‐18: 2. Linearity was checked as being r > 0.9999 between 0.02 × 10‐3 to 2 mg/ml. The determination limit (5‐fold signal/noise ratio) of the CLA reference was estimated to be 0.25, 0.50, 1.0 ng/injection for the cis/trans, trans,trans and cis,cis isomers, respectively. As expected, cis9,trans11‐18:2 was the predominant isomer (1.95 ± 0.54 mg/g fat) in beef, followed by trans7,cis9‐18:2 (0.19 ± 0.04 mg/g fat); cis,cis isomers were below the determination limit in most beef samples. Total CLA amounts determined by Ag+‐HPLC were compared to total CLAs determined by gas chromatography (GC, 100 m CPSilTM 88 column). The amounts obtained by GC were generally higher than those determined by Ag+ ‐HPLC due to co‐eluting compounds. 相似文献
8.
9.
Enzymatic enrichment of conjugated linoleic acid isomers and incorporation into triglycerides 总被引:3,自引:0,他引:3
Gerald P. McNeill Chris Rawlins Anne C. Peilow 《Journal of the American Oil Chemists' Society》1999,76(11):1265-1268
A method was developed for the enrichment of either the cis9,trans11 or the trans10,cis12 isomer of conjugated linoleic acid (CLA) from a synthetic CLA mixture consisting predominantly of these isomers in equal
amounts. Lipases were screened for their ability to selectively esterify one isomer at a significantly greater rate than the
other isomer. An immobilized lipase from Rhizomucor miehei was nonselective, but a lipase from Geotrichum candidum esterified the cis9,trans11 isomer more rapidly than the trans10,cis12 isomer. This selectivity was exploited at the kilogram scale to prepare an ester fraction with a content of 91% cis9,trans11 CLA and an unreacted free fatty acid fraction consisting of 82% trans10,cis12 CLA, based on total CLA content. The components of the reaction mixture were separated by molecular distillation. Each
enriched fraction was then incorporated into palm oil triglycerides by interesterification with the non-selective lipase from
R. miehei. Two triglyceride fats resulted, which were enriched in either cis9,trans11 CLA (26.5% cis9,trans11 and 1.7% trans10,cis12) or trans10,cis12 CLA (3.5% cis9,trans11 and 22.9% trans10,cis12). 相似文献
10.
F. Lavillonnière J. C. Martin P. Bougnoux J.-L. Sébédio 《Journal of the American Oil Chemists' Society》1998,75(3):343-352
Conjugated linoleic acid (CLA) occurs in food as a result of microbial enzymatic reactions, free radical-type oxidation, and
heat treatment. CLA is found in animal products, such as meat and dairy products, especially in cheeses. The CLA composition
of 12 different French cheeses was determined by a combination of different analytical methods: reversed-phase high-performance
liquid chromatography (RP-HPLC), gas chromatography-mass spectrometry (GC-MS), GC-Fourier transform infrared (GC-FTIR), and
silver nitrate thin-layer chromatography (AgNO3-TLC). New isomers (Δ8,10- and Δ11,13-octadecadienoic acids with all possible cis and trans configurations) that co-eluted with previously identified isomers (Δ9c,11t-; Δ9t,11c-; Δ10c,12t-; Δ10t,12c-; Δ11c,13c-; Δ9c,11c-; Δ10c,12c-; Δ9t,11t-; Δ10t12t-octadecadienoic acids) were detected. Δ9c,11t-Octadecadienoic acid was the major CLA isomer in these cheeses. All isomers were present in each product, whatever the production
process. However, CLA content in the cheeses varied from 5.3 to 15.80 mg/g of cheese fat, which depended primarily on the
origin of the milk (season, geography) and somewhat on the production process. 相似文献
11.
Effect of dietary conjugated linoleic acid (CLA) on metabolism of isotope-labeled oleic,linoleic, and CLA isomers in women 总被引:1,自引:0,他引:1
The purpose of this study was to investigate the effect of dietary CLA on accretion of 9c-18∶1, 9c, 12c-18∶2, 10t, 12c-18∶2, and 9c, 11t-18∶2 and conversion of these FA to their desaturated, elongated, and chain-shortened metabolites. The subjects were six healthy
adult women who had consumed normal diets supplemented with 6 g/d of sunflower oil or 3.9 g/d of CLA for 63 d. A mixture of
10t, 12c-18∶2-d
4, 9c, 11t-18∶2-d
6, 9c-18∶1-d
8, and 9c, 12c-18∶2-d
2, as their ethyl esters, was fed to each subject, and nine blood samples were drawn over a 48-h period. The results show that
dietary CLA supplementation had no effect on the metabolism of the deuterium-labeled FA. These metabolic results were consistent
with the general lack of a CLA diet effect on a variety of physiological responses previously reported for these women. The
2H-CLA isomers were metabolically different. The relative percent differences between the accumulation of 9c, 11t-18∶2-d
6 and 10t, 12c-18∶2-d
4 in plasma lipid classes ranged from 9 to 73%. The largest differences were a fourfold higher incorporation of 10t, 12c-18∶2-d
4 than 9c, 11t-18∶2-d
6 in 1-acyl PC and a two- to threefold higher incorporation of 9c, 11t-18∶2-d
6 than 10t, 12c-18∶2-d
4 in cholesterol esters. Compared to 9c-18∶1-d
8 and 9c, 12c-18∶2-d
2, the 10t, 12c-18∶2-d
4 and 9c, 11t-18∶2-d
6 isomers were 20–25% less well absorbed. Relative to 9c-18∶1, incorporation of the CLA isomers into 2-acyl PC and cholesterol ester was 39–84% lower and incorporation of 10t, 12c-18∶2 was 50% higher in 1-acyl PC. This pattern of selective incorporation and discrimination is similar to the pattern generally
observed for trans and cis 18∶1 positional isomers. Elongated and desaturated CLA metabolites were detected. The concentration of 6c, 10t, 12c-18∶3-d
4 in plasma TG was equal to 6.8% of the 10t, 12c-18∶2-d
4 present, and TG was the only lipid fraction that contained a CLA metabolite present at concentrations sufficient for reliable
quantification. In conclusion, no effect of dietary CLA was observed, absorption of CLA was less than that of 9c-18∶1, CLA positional isomers were metabolically different, and conversion of CLA isomers to desaturated and elongated metabolites
was low. 相似文献
12.
Jesper R. Gttsche Ellen M. Straarup 《European Journal of Lipid Science and Technology》2006,108(6):468-478
The incorporation of vaccenic acid (VA, 0.5 and 1.2%), conjugated linoleic acid (CLA, mixture of primarily c9,t11‐ and t10,c12‐CLA, 1.2%), linoleic acid (LA, 1.2%) and oleic acid (OA, 1.2%) into different tissues of mice was examined. The effects on the fatty acid composition of triacylglycerols (TAG) and phospholipids (PL) in kidney, spleen, liver and adipose tissue were investigated. VA and CLA (c9,t11‐ and t10,c12‐CLA) were primarily found in TAG, especially in kidney and adipose tissue, respectively. Conversion of VA to c9,t11‐CLA was indicated by our results, as both fatty acids were incorporated into all the analyzed tissues when a diet containing VA but not c9,t11‐CLA was fed. Most of the observed effects on the fatty acid profiles were seen in the CLA group, whereas only minor effects were observed in the VA groups compared with the OA group. Thus, CLA increased n‐3 polyunsaturated fatty acids (PUFA) in PL from kidney and spleen and lowered the ratio of n‐6/n‐3 PUFA in these tissues. Furthermore, CLA increased C22 PUFA in the PL fraction of kidney, spleen and liver, but reduced the level of arachidonic acid in PL of liver and spleen and lowered the Δ9‐desaturation indexes in all analyzed tissue TAG. 相似文献
13.
J. Fritsche R. Rickert H. Steinhart M. P. Yurawecz M. M. Mossoba N. Sehat J. A. G. Roach J. K. G. Kramer Y. Ku 《European Journal of Lipid Science and Technology》1999,101(8):272-276
The analysis, content, and daily intake of conjugated linoleic acid isomers (CLA) are presented in the following review. Modern analytical techniques such as capillary gas chromatography (GC), silverion high performance liquid chromatography (Ag+-HPLC) combined with different detection methods (flame ionisation, mass spectroscopic, ultra violet) are mandatory for the unequivocal determination of geometric and positional CLA isomers. An overview is given on the CLA contents in 139 German foods, e. g. milk/dairy products, meat/meat products, edible oils, margarines, fish, and deep fried products. The dietary intake for men and women is estimated using consumption data. 相似文献
14.
Silver-ion high-performance liquid chromatographic separation and identification of conjugated linoleic acid isomers 总被引:38,自引:21,他引:17
Najibullah Sehat Martin P. Yurawecz John A. G. Roach Magdi M. Mossoba John K. G. Kramer Youh Ku 《Lipids》1998,33(2):217-221
This is the first report of the application of silverion impregnated high-performance liquid chromatography (Ag+-HPLC) to the separation of complex mixtures of conjugated linolenic acid (CLA) isomers present in commercial CLA sources and foods and in biological specimens. This method showed a clear separation of CLA isomers into three groups related to their trans,trans, cis,trans or trans,cis, and cis,cis configuration of the conjugated double-bound system. In addition, this method separated within each geometrical isomeric group. Following Ag+-HPLC isolation, gas chromatography (GC)-electron impact mass spectrometry, and GC-direct deposition-Fourier transformed infrared spectroscopy were used to confirm the identity of two major positional isomers in the cis/trans region, i.e., Δ8,10- and Δ11,13-octadecadienoic acid, which had not been chromatographically resolved previously, Furthermore, the potential of this method was demonstrated by showing different Ag+-HPLC profiles exhibiting patterns of isomeric distributions for biological specimens from animals fed a diet containing a commerical CLA preparation, as well as for a commerical cheese product. 相似文献
15.
Conjugated linoleic acid (CLA) is a collective term that describes different isomers of linoleic acid with conjugated double
bonds. Although the main dietary isomer is 9cis,11trans-18∶2, which is present in dairy products and ruminant fat, the biological effects of CLA generally have been studied using
mixtures in which the 9cis,11trans- and the 10trans,12cis-18∶2 were present at similar levels. In the present work, we have studied the impact of each isomer (9cis,11trans- and 10trans,12cis-18∶2) given separately in the diet of rats for 6 wk. The 10trans,12cis-18∶2 decreased the triacylglycerol content of the liver (−32%) and increased the 18∶0 content at the expense of 18∶1n−9,
suggesting an alteration of the Δ9 desaturase activity, as was already demonstrated in vitro. This was not observed when the 9cis,11trans-18∶2 was given in the diet. Moreover, the 10trans,12cis-18∶2 induced an increase in the C22 polyunsaturated fatty acids in the liver lipids. The 10trans,12cis-18∶2 was mainly metabolized into conjugated 16∶2 and 18∶3, which have been identified. The 9cis,11trans isomer was preferentially metabolized into a conjugated 20∶3 isomer. Thus, the 9cis,11trans- and the 10trans,12cis-CLA isomers are metabolized differently and have distinct effects on the metabolism of polyunsaturated fatty acids in rat
liver while altering liver triglyceride levels differentially. 相似文献
16.
Sailas Benjamin Thorsten Hanhoff Torsten Brchers Friedrich Spener 《European Journal of Lipid Science and Technology》2005,107(10):706-715
To investigate the potentials of cis‐9,trans‐11 and trans‐10,cis‐12 conjugated linoleic acid (9‐CLA and 10‐CLA, respectively) isomers and their precursors trans‐11 vaccenic (VA), linoleic (LA) and oleic (OA) acids for interactions with genes, we determined their binding affinities to the ligand binding domains of the human peroxisome proliferator‐activated receptor (PPAR) α, β and γ subtypes by a fluorescent binding assay. Then, we elaborated a transactivation‐chemiluminescent assay using HepG2 cells transfected with full‐length cDNAs encoding human PPARs and retinoic acid (RA) receptors (RXRs) and with the ideal PPAR response element (iPPRE)‐luciferase reporter. Binding affinity of 9‐CLA was two times higher (but weaker than of precursor VA and OA) than that of 10‐CLA for PPARβ, the opposite was observed with PPARβ; binding affinities of CLAs and precursor fatty acids with PPARγ were comparable. Unlike in binding studies, transactivation potentials of 9‐ and 10‐CLAs were comparable in the human system. Comparing the transactivation potentials of CLAs and their precursors in toto, those obtained for PPARα (two‐ to fourfold) in both human and murine systems (the latter was used in this study as reference) were comparable, but for PPARβ and γ, fold inductions obtained in the human system were unique. Inclusion of the coactivator RXR and its natural ligand RA in the system was found unnecessary and would lead to false positive values. Taken together, the human transactivation‐based test system – which was found to be superior to the murine system – comprises only iPPRE and human PPARs for rapid screening of potential CLA and other PPAR agonists. 相似文献
17.
Incorporation of dietary linoleic and conjugated linoleic acids and related effects on eggs of laying hens 总被引:2,自引:0,他引:2
In the present study, laying hens received 29 g per kg diet of a preparation containing either 70% linoleic acid (LA) or approximately
the same amount of conjugated linoleic acid (CLA) in the control and experimental treatments, respectively. The CLA preparation
consisted predominantly of cis-9,trans-11 and trans-10,cis-12 fatty acid isomers as free fatty acids in a ratio of 1∶1. The diets were fed for 8 wk to determine the effect of dietary
CLA on quality characteristics of eggs. In addition, the fatty acid composition of liver and heart was analyzed. Performance
parameters (egg weight, feed efficiency) were not significantly affected by feeding the diets supplemented with CLA. The overall
amount of CLA that was incorporated into yolk was 7.95 g CLA/100 g total fatty acids, or approximately 400 mg CLA/egg. The
transfer efficiency of the cis-9,trans-11 isomer was higher than that of the trans-10,cis-12 isomer; however, the transfer rate of CLA isomers into yolk and tissues was significantly lower than that of linoleic
acid. Dietary CLA increased the concentration of saturated fatty acids in yolk and tissues at the expense of monounsaturated
fatty acids. The proportions of myristic, palmitic, and stearic acids in yolk lipids were also changed by dietary CLA. Additionally,
long-chain polyunsaturated fatty acids (arachidonic acid and docosahexaenoic acid) were decreased without changing the balance
of the n−6/n−3 ratio in egg yolk. The inclusion of CLA in layer diets altered the shape of the yolk and various egg parameters
(albumen height, foam index, and yolk index). The results of this study indicate that CLA induces various changes in lipid
and fatty acid metabolism of laying hens and affects quality characteristics of eggs. 相似文献
18.
Synthesis and nuclear magnetic resonance properties of all geometrical isomers of conjugated linoleic acids 总被引:1,自引:0,他引:1
Pure geometric isomers of conjugated linoleic acid were prepared from castor oil as the primary starting material. Methyl
octadeca-9Z, 11E-dienoate (2) and methyl octadeca-9Z, 11Z-dienoate (4) were obtained by zinc reduction of methyl santalbate (1, methyl octadec-11E-en-9-ynoate) and methyl octadec-11 Z-en-9-ynoate (3), respectively, as the key intermediates. Methyl octadeca-9E, 11E-dienoate (8) and methyl octadeca-9E, 11Z-dienoate (9) were prepared by demesylation of the mesyloxy derivative of methyl ricinelaidate (6, methyl 12-hydroxy-octadec-9 E-enoate). A study of the nuclear magnetic resonance spectral properties was carried out and the shifts of the olefinic carbon
atoms of 18:2(9Z, 11E) (2) and 18:2(9E, 11Z) (9) were readily identified by a combination of incredible natural abundance double quantum transfer experiment, heteronuclear
multiple bond correlation, and 1H−13C correlation spectroscopy correlation techniques. Doubts remain in the absolute identification of the individual olefinic
carbon atoms of the 18:2(9Z, 11Z) (4) and 18:2(9E, 11E) (8), except the fact that the shifts of the “inner” (C-10 and C-11) and “outer” (C-9 and C-12) positioned olefinic carbon atoms
of the conjugated diene system are distinguishable. 相似文献
19.
The relationship between growth and alterations in arachidonic acid (AA) metabolism in human breast (MCF-7) and colon (SW480) cancer cells was studied. Four different fatty acid preparations were evaluated: a mixture of conjugated linoleic acid (CLA) isomers (c9,t11, t10,c12, c11,t13, and minor amounts of other isomers), the pure c9,t11-CLA isomer, the pure t10,c12-CLA isomer, and linoleic acid (LA) (all at a lipid concentration of 16 microg/mL). 14C-AA uptake into the monoglyceride fraction of MCF-7 cells was significantly increased following 24 h incubation with the CLA mixture (P < 0.05) and c9,t11-CLA (P < 0.02). In contrast to the MCF-7 cells, 14C-AA uptake into the triglyceride fraction of the SW480 cells was increased while uptake into the phospholipids was reduced following treatment with the CLA mixture (P < 0.02) and c9,t11-CLA (P < 0.05). Distribution of 14C-AA among phospholipid classes was altered by CLA treatments in both cell lines. The c9,t11-CLA isomer decreased (P < 0.05) uptake of 14C-AA into phosphatidylcholine while increasing (P < 0.05) uptake into phosphatidylethanolamine in both cell lines. Both the CLA mixture and the t10,c12-CLA isomer increased (P < 0.01) uptake of 14C-AA into phosphatidylserine in the SW480 cells but had no effect on this phospholipid in the MCF-7 cells. Release of 14C-AA derivatives was not altered by CLA treatments but was increased (P < 0.05) by LA in the SW480 cell line. The CLA mixture of isomers and c9,t11-CLA isomer inhibited 14C-AA conversion to 14C-prostaglandin E2 (PGE2) by 20-30% (P < 0.05) while increasing 14C-PGF2alpha by 17-44% relative to controls in both cell lines. LA significantly (P < 0.05) increased 14C-PGD2 by 13-19% in both cell lines and increased 14C-PGE2 by 20% in the SW480 cell line only. LA significantly (P < 0.05) increased 5-hydroperoxyeicosatetraenoate by 27% in the MCF-7 cell line. Lipid peroxidation, as determined by increased levels of 8-epi-prostaglandin F2alpha (8-epi-PGF2alpha), was observed following treatment with c9,t11-CLA isomer in both cell lines (P < 0.02) and with t10,c12-CLA isomer in the MCF-7 cell line only (P < 0.05). These data indicate that the growth-promoting effects of LA in the SW480 cell line may be associated with enhanced conversion of AA to PGE2 but that the growth-suppressing effects of CLA isomers in both cell lines may be due to changes in AA distribution among cellular lipids and an altered prostaglandin profile. 相似文献
20.
Effects of conjugated linoleic acid isomers on lipid-metabolizing enzymes in male rats 总被引:7,自引:0,他引:7
Martin JC Grégoire S Siess MH Genty M Chardigny JM Berdeaux O Juanéda P Sébédio JL 《Lipids》2000,35(1):91-98
Male weanling Wistar rats (n=15), weighing 200–220 g, were allocated for 6 wk to diets containing 1% (by weight) of conjugated linoleic acid (CLA), either
as the 9c,11t-isomer, the 10t,12c-isomer, or as a mixture containing 45% of each of these isomers. The five rats of the control group received 1% of oleic
acid instead. Selected enzyme activities were determined in different tissues after cellular subfractionation. None of the
CLA-diet induced a hepatic peroxisome-proliferation response, as evidenced by a lack of change in the activity of some characteristic
enzymes [i.e., acyl-CoA oxidase, CYP4A1, but also carnitine palmitoyltransferase-I (CPT-I)] or enzyme affected by peroxisome-proliferators
(glutathione S-transferase). In addition to the liver, the activity of the rate-limiting β-oxidation enzyme in mitochondria, CPT-I, did
not change either in skeletal muscle or in heart. Conversely, its activity increased more than 30% in the control value in
epididymal adipose tissue of the animals fed the CLA-diets containing the 10t,12c-isomer. Conversely, the activity of phosphatidate phosphohydrolase, a rate-limiting enzyme in glycerolipid neosynthesis,
remained unchanged in adipose tissue. Kinetic studies conducted on hepatic CPT-I and peroxisomal acyl-CoA oxidase with CoA
derivatives predicted a different channeling of CLA isomers through the mitochondrial or the peroxisomal oxidation pathways.
In conclusion, the 10t,12c-CLA isomer seems to be more efficiently utilized by the cells than its 9c,11t homolog, though the Wistar rat species appeared to be poorly responsive to CLA diets for the effects measured. 相似文献