冠状动脉结扎制备大鼠心肌梗死模型及血流动力学检测

目的 探讨大鼠心肌梗死模型制备及血流动力学检测方法.方法 60只雄性SD大鼠随机分为模型组、假手术组,分别行左冠状动脉前降支结扎术和假手术,术后2周行血流动力学检测及心脏组织病理学检查.结果 模型组大鼠左室收缩压(LVSP)、左室压最大上升和下降速率(±dp/dtmax)明显低于假手术组(P＜0.05或0.01),左室舒张末压(LVEDP)则高于假手术组(P＜0.01),HE及Masson染色检查显示模型组心肌均出现梗死,造模成功率100%;假手术组大鼠心肌无梗死形成.结论 左冠状动脉前降支结扎术制备心肌梗死模型简单易行.     

丹酚酸B调节胆固醇代谢的研究

[目的]研究丹酚酸B对血液胆固醇代谢的调节作用.[方法]选取SD大白鼠,建立高脂模型,进行丹酚酸B干预试验,测定大鼠血液及粪便胆固醇与胆汁酸含量.[结果]丹酚酸B干预的大鼠血液中胆固醇含量显著低于高脂模型组大鼠,粪便中胆固醇及胆汁酸含量显著高于高脂模型组.[结论]丹酚酸B主要通过促进粪便胆固醇与胆汁酸的排泄降低血液中胆固醇.     

黄连解毒汤对大鼠慢性脑灌注不足损伤的保护作用

目的:观察黄连解毒汤对大鼠慢性脑灌注不足损伤的保护作用.方法:将24只雄性SD大鼠随机分为假手术组、模型组及黄连解毒汤治疗组(治疗组)(均n＝8).采用双侧颈总动脉永久性阻断法制备模型,术后灌胃给予黄连解毒汤(4.0 g生药/kg体重).连续治疗60 d后,用Morris水迷宫测试大鼠学习记忆能力;用HE染色法检查脑白质病理改变并定量检测胼胝体神经纤维脱失变化.结果:与假手术组比较,模型组大鼠隐匿逃避潜伏期(escape latency,EL)显著延长(P<0.01),穿越平台次数明显减少(P<0.01);与模型组比较,治疗组大鼠EL明显缩短(P<0.01),穿越平台次数显著增加(P<0.05).模型组大鼠可见脑白质损伤改变,胼胝体白质囊泡面积占测量总面积的百分比值显著高于假手术组(p<0.01).与模型组比较,治疗组脑白质损伤明显减轻,胼胝体白质囊泡面积占测量总面积的百分比值显著降低(p<0.01).结论:黄连解毒汤能够改善慢性脑灌注不足大鼠的脑白质损伤与学习记忆能力.     

人参三七提取物抗疲劳作用的研究

[目的]研究人参三七提取物缓解体力疲劳的作用,为进一步开发利用人参三七提供试验依据.[方法]将小鼠分为4批,每批48只,随机分为4组,分别为对照组和50、100、300 mg/kg 3个给药组,各组小鼠经口灌胃给药30 d后,分别测定其负重游泳时间及血清尿素氮、血乳酸、肝糖原含量.[结果]100和300 mg/kg 剂量组人参三七提取物小鼠负重游泳时间显著长于对照组(P<0.01),血清中尿素氮含量显著低于对照组(P<0.01;P<0.05),小鼠肝糖原水平明显高于对照组(P<0.01;P<0.05);小鼠游泳后血乳酸含量显著低于对照组(P<0.01;P<0.05).[结论]人参三七提取物具有缓解体力疲劳的作用.     

老年急性心肌梗死的临床分析

目的:探讨老年性急性心肌梗死的治疗措施及临床特征;方法:选择2009年2月至2011年2月我院收治的老年性急性心肌梗死患者61例为观察组,选择同期我院收治的老年性急性心肌梗死患者61例为对照组,对两组的临床特征及疗效进行比较;结果:两组首发症状的胸痛、胸闷和心悸相比差异有显著性(P<0.05),两组梗塞部位的非Q波相比差异有显著性 (P<0.05),两组治疗效果相比差异有显著性(P<0.05);结论:应针对患者的不同情况进行个体化治疗,提高患者生活质量.     

HO/CO在糖尿病肾病大鼠肾脏组织中的表达及其意义

目的:观察糖尿病肾病模型大鼠血浆一氧化碳(CO)的变化和血红素氧合酶-1(HO-1)、血红素氧合酶-2(HO-2)在肾脏组织的表达,探讨血红素氧合酶/一氧化碳(HO/CO)系统在糖尿病肾病发生和发展中的作用.方法:将24只SD大鼠随机分为对照组和模型组,按Anderson等方法建立糖尿病肾病模型.8周后收集尿液测定24 h的尿蛋白(Upro)和尿肌酐(Ucr);大鼠断头取血测定CO、尿素氮(BUN)和肌酐(Scr),计算肌酐清除值(Ccr);剖取肾脏组织,采用免疫组织化学法检测肾脏组织HO-1和HO-2的表达.结果:与对照组比较,模型组大鼠Upro、BUN和Scr明显升高(P<0.01),Ccr降低(P<0.01);模型组大鼠血浆CO与对照组比较明显降低(P<0.01),肾脏组织HO-1和HO-2的表达强度显著减弱(P<0.01).结论:HO/CO的调节失常参与了糖尿病肾病的发生和发展.     

阳离子A对内毒素损伤大鼠肝脏中HSP70表达的影响

[目的]运用免疫组织化学技术研究阳离子A(CA)对HSP70在内毒素(ET)损伤大鼠肝脏中表达的影响.[方法]48只SD大鼠随机分为2组:对照组和CA+ET组.每组各24只.对照组大鼠分别经尾静脉注射ET 5 mr/kg(BW),CA+ET组尾静脉注射1 g/kg(BW)CA溶液,之后2 min内尾静脉注射ET 5 mg/kg BW.2组分别在3、4、8、12 h采集肝脏作为检测样本,应用HE染色和免疫组织化学染色技术进行检测.[结果]肝细胞病理变化在ET攻击后3和4 h时明显增多.4 h达到峰值,而在8和12 h时逐渐下降.CA+ET保护组肝细胞的细胞病变较ET组同时间段有所减轻;2组HSP70的积分光密度在3、8、12 h差异极显著(P<0.01),4 h差异显著(P<0.05),且对照组HSP70积分先密度随时间的延长而下降,而CA+ET组HSP70的积分光密度在3、4、8 h这3个时间点呈下降趋势,在12 h小幅度上升.[结论]CA能显著上调HSP70在肝脏的表达,从而对由ET诱导的肝损伤产生保护效应.     

红茶提取物对猪肉品质及生化指标的影响

[目的]研究红茶提取物对猪肉品质及生化指标的影响.[方法]选60头50 kg左右的杜长大肥育猪,随机分为时照组和试验组,分别饲喂基础日粮和基础日粮+2 g/kg红茶提取物.至猪体重达100 kg左右时,测猪肉的各项指标.[结果]试验组猪的屠宰性能提高,屠宰率显著提高(P<0.05);猪肉品质得到显著改善,肉色显著改善(P<0.05),肌肉丰度极显著提高(P<0.01);生化指标得到改善,肌苷酸显著提高(P<0.05),胆固醇显著下降(P<0.05),铁、锌含量显著提高(P<0.05).[结论]饲料中添加2 g/kg红茶提取物可改善猪肉品质.     

CCB对大鼠坐骨神经嵌压性损伤的保护作用及其机制初探

目的:探讨:①急性周围神经嵌压性损伤早期c-fos基因的表达及其对神经病理及神经传导功能的影响;②Ca2+通道阻滞剂(CCB,氟桂利嗪)对此的干预效应及机制.方法:制作坐骨神经嵌压性损害的大鼠动物模型,予模型大鼠腹腔分别注射氟桂利嗪1mg/Kg、2mg/Kg,或生理盐水5ml/Kg.在嵌压后0、5min、15min、30min、1h、2h和4周时取大鼠坐骨神经、背根神经节(DRG),采用RT-PCR、病理学、电生理学等方法测定各时点DRG c-fos mRNA水平和第4周时大鼠背根神经节病理变化、坐骨神经传导速度(NCV)和足趾间距,并与对照组比较.结果:①生理盐水组、氟桂利嗪低剂量(FⅠ)和氟桂利嗪高剂量(FⅡ)组坐骨神经c-fos mRNA表达于损伤30min时开始增加、1h达高峰后开始下降、2h时仍高于对照组(P<0.01);其中生理盐水组>FⅠ组>FⅡ组(P<0.05/P<0.01).②损伤4周时,光镜下DRG病理变化严重程度顺序为:生理盐水组>FⅠ组>FⅡ组,对照组未见异常.③损伤4周时坐骨神经NCV,生理盐水组和FⅠ组显著慢于FⅡ组和对照组(P<0.01),FⅡ组与对照组间差异无统计学意义(P>0.05).④损伤4周时大鼠足趾间距,生理盐水组均明显小于其他3个组(P<0.01);FⅡ组、FⅠ组与对照组间相互差异均无统计学意义(P>0.05).结论:周围神经嵌压性损害后发生神经病损和神经功能障碍,这可能与c-fos基因表达上调有关;早期应用CCB(氟桂利嗪)可减轻神经损伤、促进神经功能恢复,这可能与CCB阻断了Ca2+内流过程使早期神经细胞c-fos基因的表达下调有关.     

野生蒲公英联合高山红景天对糖尿病模型小鼠的降血糖作用研究

[目的]探讨长白山野生蒲公英(Taraxacum mongolicum)联合高山红景天(Rhodiola sachalinensis)对糖尿病小鼠的降血糖影响.[方法]选取30只昆明种小鼠,其中24只造成糖尿病模型,分为5组,分别为正常对照组、四氧嘧啶模型组、高山红景天组、长白山野生蒲公英组和联合剂量组.采用自制的水煎液进行灌胃给药,1次/d,连续5 d,测定糖尿病模型小鼠的血糖值.[结果]给药5 d后,各试验组小鼠血糖浓度均明显降低,与四氧嘧啶模型组相比存在极显著差异(P<0.01).[结论]高山红景天、长白山野生蒲公英、高山红景天与蒲公英联合对糖尿病小鼠都有明显的降血糖作用.     

转炉炉壳热应力分析

根据实际转炉建立实体模型。以有限元为手段,考虑了炉衬和炉壳材料的物性参数随温度变化的特点和炉衬与炉壳之间膨胀间隙,计算了转炉炉壳在温度载荷和炉衬膨胀压力同时作用下的热应力。所得结果可为转炉炉壳设计提供依据。     

焦炭热性能指标意义浅析

通过对焦炭在高炉内的历程及其变化规律的剖析,阐述焦炭热性能指标的意义,形象地把焦炭在高炉内的损耗分为三部分(M1,M2,M3)。指出M2对焦比及焦炭价格有一定的指导作用。     

Results of treatment of cancer of the cardia

Cancer of the cardia is traditionally discussed with cardiac cancer of the lower portion of the esophagus and upper gastric cancer invading the esophagus, and the specific characteristics of cancer of the cardia have never been clearly defined. We reviewed the outcome of 172 patients with adenocarcinoma of the cardia who had undergone radical surgery between 1949 and 1994 in the Division of Gastrointestinal Surgery of the Cancer Institute Hospital. The centers of the tumors were located within 2 cm above and below the boundary between the esophagus and the stomach, and their longitudinal diameter was less than 8 cm. We divided the patients into an early period (1949-1979; n = 79) and a late period (1980-1994; n = 93), and focused on the historical transition. There were no differences in patient gender or histology between the two periods. However, the late period was associated with fewer cases of esophageal invasion and shorter longitudinal diameter, as the age of the population advanced. The number of advanced cancers such as the localized and infiltrative type had decreased, and early cancer and early clinical stage had become more common, but despite the fact that the number of early cancers had increased, extended dissection, such as thoracic and paraaortic lymph node dissection, was performed more frequently. By clinical stage, the long-term outcome markedly improved in Stage I patient in the late period, and tended to improve in Stage II and III patients. This appears to have been attributable to the prevention of micrometastasis by extended dissection, although the number of early cancers is another major potential cause. There were no differences in the outcome of Stage IV patients between the two periods, and further advances in multimodality therapy must be awaited. The range of resection is basically proximal gastrectomy, and if there are adequate indications, the prognosis is favorable. In view of the status of lymphatic flow and lymph node metastasis, and long-term results, lateroaortic lymph node dissection is important. Since the number of early cancer patients has been increasing, if intraabdominal recurrence is prevented, intrathoracic lymph node dissection will contribute greatly to the outcome of such patients.     

The effect of amount of possession of the trait of hostility on accuracy of perception of hostility in others.

Some theories of personality hold that an individual's perception of others is influenced by his own personality characteristics; more specifically, that a trait of low social value will be projected more into others, and that insight into the possession of these characteristics reduces this distortion. These 2 hypotheses were tested by having a group of college students rate each other (including themselves) on degree of friendliness or hostility. Agreement with group rating about self yielded the insight score. In general, the hypotheses were supported; the "friendly" Ss were most accurate in their perception of others, and insight was positively associated with accurate perception. From Psyc Abstracts 36:04:4HL16M. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Comparison of rates of infection of two methods of emergency ventricular drainage

The rates of infection of two methods of external ventricular drainage in use at Atkinson Morley's Hospital--namely, (a) percutaneous drainage with Rickham reservoirs and (b) tunnelled ventriculostomies--were compared in this retrospective review. Percutaneous drainage of CSF with Rickham reservoirs was associated with a 27% rate of infection as identified by positive microbiological cultures; tunnelled ventriculostomy catheters had a 10% infection rate. The difference in the infection rate between the two methods was statistically significant (P < 0.015). Other variables examined, including the age and sex of the patients and the reasons for ventricular drainage, were not associated with an increased rate of infection. Most infections from either method were caused by a coagulase negative staphylococcus. The average duration of ventricular drainage before identification of positive cultures was 5.7 days for Rickham reservoirs and 6.0 days for ventriculostomies.     

浅析《野草》的版画特征

鲁迅先生一生热爱美术作品,对版画中木刻兴趣尤甚.<野草>作为其"写心之作",亦呈现出一些与木刻版画一致的审美特征:一是具有木刻版画的色彩特征,多对比、多冷色,有阴郁的美感;二是具有木刻版画的力度特征,环境描写、人物刻画处处刀锋毕现,遒劲有力,有一种"力之美".     

四辊破碎机车刀架的改造

针对四辊破碎机车刀架车削中存在的几个问题,详细介绍了电动车刀架的结构和工作原理,建立主要设计参数的数学表达式,并进行适当的分析。改造后,这种电动车刀架运行平稳、可靠、方便、省力,取得了预期的效果。     

Effect of antibiotics of chemotaxis of human leukocytes

The effect of 20 different antibiotics on chemotaxis by human neutrophils was studied. Human leukocytes incubated with chloramphenicol, rifampin, sodium fusidate, and tetracyclines in vitro showed markedly depressed migration. The mechanisms by which these antibiotics affect leukotaxis are discussed.