The effect of ionising radiation on the colour of leg and breast of poultry meat

The effect of irradiation (0 and 5 kGy) of chicken, goose and turkey breast and leg muscles and subsequent storage at 4°C was studied in relation to colour changes. The colour of the outside surface was measured on the breast on each day of storage for up to 7 days post irradiation and for breast and leg and day 7. The colour of a freshly cut interior surface of both breast and leg was measured after 7 days storage. L* values of control and irradiated chicken, goose and turkey breast muscles changed little during storage post irradiation. The a* values for unirradiated goose breast were significantly higher than irradiated goose breast but declined to values similar to irradiated goose breast after 7 days of storage. The b* values for irradiated turkey breast were significantly higher than unirradiated turkey breast at all times post irradiation treatment. Analysis of variance was performed on the day 7 CIELAB values of breast muscle for the effects of species, surface and irradiation and their interactions. After 7 days storage a* values of poultry breast were higher on the freshly cut surface due to irradiation in all species, with decreases in hue angle due to irradiation. The a* values of leg of all species at 7 day post irradiation was significantly higher in the irradiated treatment than the controls. The results for the turkey leg indicate that this effect may be mainly due to higher a* values of the freshly cut surface. The possible role of carboxy form of the haem pigments as the irradiated pigment form is discussed.     

The use of discriminant analysis for the interpretation of the reflectance spectra of irradiated porcine M. longissimus dorsi

Slices of porcine M. longissimus dorsi were packed in overwrap packs and subjected to irradiation (0 and 5 kGy) and then stored for 7 days at 4°C. Reflectance spectra were measured on the outside surface and a freshly cut surface at 7 days post irradiation. The reflectance spectra were transformed to reflex attenuance, k/s and first and second difference spectra and subjected to discriminant analysis. Using discriminant analysis it was possible to establish a calibration equation to discriminate between the spectra of irradiated and unirradiated pork for both the outside and the inside surface. When the calibration model was used to predict the classification of new samples a 100% correct grouping was obtained for the freshly cut surface, however, for the outside surface the classification ranged from 87 to 100% correct depending on the mathematical transformation of the reflectance spectra. This shows the potential of colour measurements as a possible rapid initial screening test for the identification of irradiated pork. Evaluation of the first difference spectra to identify peak positions showed significant differences in peak positions between irradiated and unirradiated pork. The position of the peaks in the irradiated sample is discussed as lending support to the hypothesis of the carboxyhaem form as the irradiated pigment.     

Effect of residual oxygen on the colour, odour and taste of carbon dioxide-packaged beef, lamb and pork during short term storage at chill temperatures

Samples of boneless pork, lamb, beef (high and normal pH) were packaged in '100%' carbon dioxide atmospheres in foil laminate pouches. These pouches were fitted with a septum and a gas sampling port that allowed the introduction of air and removal of gas samples for analysis from the sealed packs. After sealing, measured volumes of air were introduced into test packs that had been gassed at a carbon dioxide volume to meat weight ratio of either 1 litre/kg or 2 litres/kg, to give initial atmospheres containing approximately 0·1, 0·2 and 1·0% oxygen. After 24 and 168 h storage at -1·5 ± 0·5°C, test packs were removed and compared with similarly treated control packs without added oxygen with respect to meat odour, taste and colour. No significant differences between the test and control packs in respect to odour or taste were evident with any meat type. The tendency to develop browning in response to the presence of residual oxygen within packs, increased in the order: pork, normal pH beef, normal pH lamb, high pH beef. Beef and lamb developed noticeable browning in packs containing more than 0·15% total oxygen while pork was able to tolerate 1% oxygen without obvious detrimental effects. For all meat types, the colour stability was greater in packs gassed to the higher gas volume to meat weight ratio.     

A comparison of modified atmosphere and vacuum skin packing for the storage of red meats

The performances of two commercially available packaging systems for prolonging the shelf-life of fresh meat were compared at 1°C and during simulated retail display. Beef and pork loin steaks in modified atmosphere packs (MAP), containing 75% O2 and 25% CO2, developed an initial bright red (beef) or pink (pork) colour, which gradually changed to brownish-red or -pink after 12 days; similar samples in vacuum skin packs (VSP) remained purple-red throughout the storage period. Off-odours developed more rapidly in MAP (8–12 days), possibly due to more extensive growth of Brochothrix thermosphacta and the effects of aerobic conditions on the metabolites of lactic acid bacteria, which predominated in both types of packs. Evidence of rancidity, using thiobarbituric acid assay, was demonstrated in MAP beef after approximately 8 days, but not in VSP. Drip losses in MAP increased after 6 days' storage, but remained generally low in VSP. Physical texture (shear force values of cooked samples) of beef was unaffected by packaging method, but pork in VSP was significantly more tender ( P < 0.001) than in MAP.     

Colour stability of six beef muscles stored in a modified atmosphere mother pack system with oxygen scavengers

Summary The effect of O₂ scavengers on the colour stability of beef in retail overwrap trays within a modified atmosphere mother pack (CO₂/N₂) was assessed. Steaks from six muscles, namely Longissimus dorsi, Psoas major, Semimembranosus, Gluteus medius, Semitendinosus and Biceps femoris were examined. After storage for 2, 4 or 6 weeks mother packs were opened and steak colour was monitored during 96 h of retail display. Redness of all muscles stored with O₂ scavengers was superior to that of steaks stored without O₂ scavengers at all storage times. Hue angle results indicated some metmyoglobin formation in all muscles during storage. Comparisons were made between steaks stored with O₂ scavengers and fresh steaks. Shelf-life values were calculated using the reflectance difference method (R₆₃₀–R₅₈₀). O₂ scavengers did not affect weight loss from the stored steaks.     

Evaluation of animal age and muscle type on beef log juice colour and residual GOT activity when cooked to target end-point temperature of 79.4 °C†

The effectiveness of the pink-juice test to determine 79.4 °C end-point temperature (EPT) of cooked beef logs was appraised. Logs made from five cuts of meat from three animal age groups were fabricated in triplicate to simulate products received at ports of entry and then cooked to EPTs of 78.0–81.8 °C. CIELAB L*, a*, b*, C and h ° values of internal surfaces of freshly sliced meat and pressed juices from samples were determined. Sensory ratings were made by six trained panelists to determine intensities of residual red color of the juices. Animal age and cut of meat had little effect on the CIELAB color values. Residual red color was apparent in all samples by both sensory and a* values analysis. Sensory ratings did not vary significantly (P < 0.05) by animal age or cut of meat. Average values of 3.2 (intensity range, 1–9) corresponded to an EPT slightly greater than 80 °C; absence of red color in the juices would therefore indicate an EPT in excess of the target temp of 79.4 °C. Residual glutamic-oxaloacetic transaminase activity (GOT) activity, used as a collateral test for EPT, ranged from 482.5 to 1641.8 SFU ml⁻¹ of juice and differed significantly by cut of meat (P < 0.05), but not by animal age. © 1999 Society of Chemical Industry     

Display, Packaging, and Meat Block Location Effects on Color and Lipid Oxidation of Frozen Lean Ground Beef

Ground beef chuck (15% fat) was packaged (500g) in vacuum bags, Saran Wrap^TM overwrapped with aluminum foil or polyvinyl chloride (PVC), exposed to retail display light (4°C) for 24 hr and then held frozen (?18°C) for 52 wk. Instrumental and visual color of PVC-packaged beef was affected most by display and by frozen storage. During frozen storage, as oxygen permeability of packaging material increased, TBA, visual brownness, and metmyoglobin increased while visual redness, acceptability, a* value, red color contributed by oxyrnyoglobin, and deoxymyoglobin decreased. Location on the meat block (exterior vs interior) affected (p<0.05) visual red and brown color, lightness, and acceptability, L*, a*, and b*, and TBA values.     

冷却猪肉新鲜度的色差快速分析评价方法

为了得到冷却猪肉新鲜度的变化规律,本研究以冷却猪肉里脊部分为研究对象,进行球蛋白沉淀实验、感官评价、pH值、水分含量、肉浸液以及肌肉表面颜色值测定。结果表明：球蛋白沉淀实验、pH值能够较好地反应肉品新鲜度的变化,色差分析符合一定的规律：肌肉表面色差测定中,20℃条件下,a*变化与贮藏时间显著相关(P＜0.05),L*变化显著(P＜0.05);4℃条件下,b*、C变化与贮藏时间极显著相关(P＜0.01),L*值与pH值极显著相关(P＜0.01);对于肉浸液,L*、b*、C、ΔE*均发生显著性变化(P＜0.05),20℃条件下L*、b*、C、ΔE*与贮藏时间和pH值显著相关(P＜0.05),而4℃条件下,L*、C、ΔE*与贮藏时间显著相关(P＜0.05),L*、a*、b*、C、ΔE*值与pH值极显著相关(P＜0.01);冷链过程中,当L*高于48.73、a*高于4.72、b*高于9.18时可认定冷却肉为新鲜肉。     

Influence of different gas compositions on the short-term storage stability of mother-packaged retail-ready lamb packs

Longissmus dorsi muscles were removed from Suffolk cross-breed lambs (aged 4-9 months) and cut into steaks. Lamb steaks were over-wrapped on trays and placed in vacuum pack bags. Bags were divided into 3 groups and flushed with gas mixtures containing 100:0, 90:10 or 80:20/CO(2):N(2). Mother packed lamb bags were stored for 4 days (T2) and 7 days (T3), respectively, in darkness at 4 °C, prior to retail display. The effect of aerobic packaging alone on lamb meat quality was used as the control (T1). Under retail display, all over-wrapped trays were held under refrigerated conditions (4 °C, 616 lx) for up to 8 days. Steaks were assessed for microbial growth, oxidative and colour stability as well as pH every 2 days. Mother-packing in 100:0/CO(2):N(2) was the most effective way of extending the storage life of retail ready lamb prior to display, particularly over longer storage periods. TVCs for T3 lamb meat using all gas compositions remained below 2.0×10(6) CFUs/g meat up until day 6 compared to day 4 in both T1 and T2 lamb. Lipid oxidation in lamb mother-packed for 7 days occurred at a faster comparative rate than discolouration and microbial growth and was the major determinant of shelf-life. However, under simulated retail display in aerobic packages, TBARS values did not increase significantly. There was no significant difference between Hunter 'a' values for T3 lamb meat and the control, but T3 meat mother-packed in 100:0/CO(2):N(2) had higher 'a' values than those of the control and T3 meat packed in other gas compositions. Lamb steaks in T3 previously mother-packed in 100:0/CO(2):N(2) were also significantly (p<0.05) higher than those of T2 on day 0. T3 meat also maintained initial colour values over those of the control.     

Physical characteristics of lamb primals packaged under vacuum or modified atmospheres

Lamb primals (shoulders) were packaged under vacuum, 80% O(2)/20% CO(2), 50% CO(2)/50% N(2) or 100% CO(2) and stored at 5 or 0 °C. Pack contents were examined at 7 day intervals to determine the composition of the pack atmosphere, drip loss, colour (muscle and fat) and pH (surface and internal). The composition of the gas atmospheres changed very little during storage. The only significant differences between developed head space compositions above primals stored at the two different temperatures (5 and 0 °C) were noted in packs stored for 28 days under 80% O(2)/20% CO(2). Low levels of drip loss (<0.5%) were noted in all packs stored under the modified gas atmospheres. In contrast, significantly higher levels of drip loss (0.5-1.1%) were noted in vacuum packaged lamb stored at 5 and 0 °C. Acceptable muscle colour was observed 2 hr after opening of all packs. The only significant differences between atmospheres for lean muscle colour were noted after 28 days storage. Fat colour did not generally change during storage in any of the atmospheres, apart from a slight bleaching effect at 7 days. There were no significant differences between the surface or internal pH values noted after storage under any of the atmosphere/temperature combinations. In general, higher pH values were observed at the surface of the meat than in the interior. This pattern was noted before and after storage.     

A second mutant allele (V199I) at the PRKAG3 (RN) locus-II. Effect on colour characteristics of pork loin

Three alleles at the PRKAG3 (RN) locus that influence the glycogen content of pork were found to be segregating in Hampshire×Landrace crossbred pigs, RN(-), rn(+) as well as second mutant allele V 199I (here denoted rn*). The effect of these three alleles on ultimate pH, pigment content, internal reflectance (FOP), surface colour measured by tristimulus colorimetry (L*, a*, b*) and fractions of deoxymyoglobin (Mb), oxymyoglobin (MbO(2)) and metmyoglobin (MetMb) of pork loin was studied. Moreover, the effect of sex, entire male versus female pigs, on these traits was also analysed. The three PRKAG3 alleles affected ultimate pH, internal reflectance, colour and distribution of myoglobin derivatives of pork loin, while the pigment content was not influenced. Ultimate pH values of loins from the three genotypes were found to be in the order RN(-)/- genotypes rn(+)/rn(+) genotype=rn(+)/rn* genotype=rn*/rn* genotype. The RN(-) allele was dominant resulting in higher redness (a* value) and yellowness (b* value), while the rn* allele tended to result in lower redness and yellowness compared with the rn* allele. The RN(-) allele was dominant over the rn* allele in lightness (L* value) giving a lighter colour. Surface colour differences were mainly explained by differences in the distribution of the myoglobin derivatives. Finally, surface lightness was higher and pigment content, redness and fraction of MbO(2) lower in loin from entire males compared with females.     

Continued inhibitory effect of carbon dioxide packaging on Listeria monocytogenes and other microorganisms on normal pH beef during abusive retail display

Beef steaks of normal pH (5.3–5.5) were inoculated with Listeria monocytogenes , individually packaged in saturated carbon dioxide atmosphere packaging (SCAP) for <3 h or 5 or 8 weeks or in vacuum packaging (VP) for <3 h, and stored at −1.5°C. After each storage period, 27 individually packaged steaks were removed from their storage packs, overwrapped and placed on retail display under conditions simulating gross temperature abuse (12.25°C). Other steaks were removed from their storage packs and rinsed to remove L. monocytogenes cells, which were re-inoculated onto freshly cut beef steaks to simulate cross-contamination. These crosscontaminated steaks were overwrapped and also subjected to abusive display. Meat pH did not change significantly during storage or retail display. During the retail display of steaks previously stored in SCAP, the lag phase of aerobic bacteria and lactic acid bacteria was longer after prolonged storage compared to short (<3 h) exposure to carbon dioxide. For the same samples, L. monocytogenes failed to grow during retail display or grew only slightly after a prolonged lag phase (>75 h), even after only brief exposure time (<3 h) to carbon dioxide. In contrast, with cross-contaminated steaks, when the inocula had been exposed to SCAP or VP for a short time (<3 h) the L. monocytogenes lag phase was shorter (<20 h). Inocula from steaks stored in SCAP for 5 or 8 weeks did not grow on the cross-contaminated steaks. It is concluded that exposure of both the beef substrate and the L. monocytogenes inoculum to carbon dioxide during prolonged chilled storage does not increase the risk of growth of L. monocytogenes when that meat is subsequently placed on retail display, nor is there a large risk of growth of L. monocytogenes where cross-contamination from SCAP stored raw beef to fresh raw beef occurs prior to retail display.     

Efficacy of cetylpyridinium chloride against Listeria monocytogenes and its influence on color and texture of cooked roast beef

Sliced (cut) and exterior (intact) surfaces of restructured cooked roast beef were inoculated with Listeria monocytogenes, treated with cetylpyridinium chloride (CPC; immersion in 500 ml of 1% solution for 1 min), individually vacuum packaged, and stored for 42 days at 0 or 4 degrees C. Noninoculated samples were similarly treated, packaged, and stored to determine effects on quality (color and firmness) and on naturally occurring bacterial populations, including aerobic plate counts and lactic acid bacteria. Immediately after CPC treatment, regardless of inoculation level, L. monocytogenes populations were reduced (P = 0.05) by about 2 log CFU/cm2 on sliced surfaces and by about 4 log CFU/cm2 on exterior surfaces. Throughout 42 days of refrigerated storage (at both 0 and 4 degrees C), L. monocytogenes populations on CPC-treated samples remained lower (P = 0.05) than those of nontreated samples for both surface types. After 42 days of storage at both 0 and 4 degrees C, aerobic plate count and lactic acid bacteria populations of treated samples were 1 to 1.5 log CFU/cm2 lower (P = 0.05) than those of nontreated samples for both surface types. CPC treatment resulted in negligible effects (P > 0.05) on the color (L*, a*, and b* values) of exterior and sliced roast beef surfaces during storage. For both sliced and exterior surfaces, CPC-treated samples were generally less firm than nontreated samples. CPC treatment effectively reduced L. monocytogenes populations on roast beef surfaces and resulted in relatively minor impacts on color and texture attributes. CPC treatment, especially when applied to products prior to slicing, may serve as an effective antimicrobial intervention for ready-to-eat meat products.     

Effects of vitamin E supplementation on fattening performance, non-carcass components and retail cut percentages, and meat quality traits of Awassi lambs

This experiment was carried out to evaluate the effects of vitamin E supplementation on growth, non-carcass components and retail cut percentages, and meat quality traits of Awassi male lambs at approximately 8 months of age. The lambs were divided into two groups as control (CG, n=12) and experimental (VG, n=12) at the beginning of the fattening period. The CG and VG lambs were fed with a concentrate and grass hay close to ad-libitum by biweekly adjustment of the amount offered. In addition, the VG received a supplement of 45 mg vitamin E per lamb per day during a 75-day fattening period. Inital weight, final weight, daily weight gain and feed conversion efficiency were 31.8±1.40 kg, 45.5±1.37 kg, 183±13 g and 7.6 for CG, 32.5±1.45 kg, 46.7±1.42 kg, 189±15 g and 7.0 for VG, respectively. Vitamin E supplementation did not have a statistically significant effect on animal performance traits, non-carcass components and retail cut percentages, but produced an 8.1% improvement in feed conversion efficiency. After slaughter, carcasses were chilled at 4?°C for 24 h. Then, the carcasses were dissected into wholesale cuts, and m. longissimus dorsi (LD) muscles excised. The samples of muscle were subjected to moisture, protein, ether extract and ash analyses. Samples were cooked for shear test and cooking yield measurements. There were no significant differences between CG and VG lamb groups in chemical composition of meat samples from the LD muscles. Though the influence of vitamin E supplementation on color parameters (L*, a*, b*) was not statistically significant, the mean a* (redness) values decreased on days 2 and 4 and increased on days 7 and 12 of the storage period. However, the a* values of muscles from the VG were higher than those grouping CG. L* and a* values in LD muscle from vitamin E-treated lamb groups were also preserved for a period of 12 days of maturation. In this study, drip loss was relatively preserved by vitamin E supplementation to the diet of animals. The results showed that vitamin E supplementation to the diet of Awassi male lambs at an inclusion rate over the amount of nutritional recommendations relatively reduced lipid oxidation, drip loss and tended to maintain meat redness.     

Retail Shelf-Life of Pork Dipped in Organic Acid before Modified Atmosphere or Vacuum Packaging

Modified atmosphere packaging (MAP) is increasingly popular for meat, but raw, chilled pork in vacuum or anoxic environments has a purple color. The retail shelf‐life of pork chops dipped in 500 ppm ascorbic acid, 250 ppm citric acid, or no acid dip and stored at 1 °C before simulated retail display in MAP with gas exchange or air‐permeable packaging after vacuum pouch storage was determined. The 80% N₂:20% CO₂ in MAP was exchanged with 80% O₂:20% CO₂, and chops were removed from vacuum packages and overwrapped with permeable film (VP‐PVC) on the 7th day before simulated retail display at 4 °C. Shelf‐life traits were determined at 1, 7, 8, 10, 12, and 14 d postpackaging. The pH values changed with time, but returned to post‐dipped, prepackaged levels at the end of simulated retail storage. Weight loss of chops increased (P < 0.05) in VP‐PVC compared with MAP. The a* values increased (P < 0.05) and L* and b* values decreased during simulated retail display, with higher L*, a*, and b* color values for chops in MAP than VP‐PVC. Log numbers of psychrotrophic microorganisms were higher (P < 0.05) on VP‐PVC samples than for chops in MAP on days 12 and 14. Psychrotrophic counts on ascorbic acid‐treated samples were decreased compared with citric acid or no dipping on pork during simulated retail display. Pork chops in MAP with gas exchange had lighter and redder color, increased weight retention, decreased psychrotrophic counts, and increased lipid oxidation compared with conventional vacuum and overwrap packaging systems.     

Impact of introducing specifications on the tenderness of retail meat

Over a 3-year period (1997-1999), the shear force of 4371 retail beef, lamb and pork midloin samples collected from 363 retail outlets were tested using a MIRINZ tenderometer. Information about aging time, processor and retail chain was recorded. Consumers (n=2313) were also surveyed on their perception of the tenderness of beef and lamb midloin samples with known shear force. The results validated that shear force, as measured by the MIRINZ tenderometer, could be used to create instrumental tenderness categories which reflected consumer perceptions of tenderness. Over the 3-year sampling period, the shear force of beef and lamb decreased by 21.9 and 17.2%, respectively, and there was a consistent decrease in the number of 'tough' samples. The improvement in tenderness coincided with the introduction of a Quality Mark program in 1997 for beef and lamb and 3 years of implementation by auditing. The Quality Mark program sets specifications for the quality of retail meat in New Zealand and guidelines to achieve these specifications. In comparison to retail beef and lamb, the shear force of retail pork decreased marginally by 7.9%. Furthermore, the decrease in the number of 'tough' pork samples was not consistent over the testing period. Analysis of these data showed that for all three meats a considerable improvement in tenderness can be achieved by adopting a minimum post-slaughter aging time and optimizing the processing conditions.     

Color Characteristics of Irradiated Vacuum-Packaged Pork, Beef, and Turkey

Changes in color of irradiated meat were observed to be species-dependent. Irradiated pork and turkey became redder due to irradiation but irradiated beef a* values decreased and yellowness increased with dose and storage time. The extent of color change was irradiation dose-dependent and was not related to myoglobin concentration. Visual evaluation indicated pork and turkey increased in red ness whereas beef decreased in redness as dose levels increased. Reflectance spectra showed that irradiation induced an oxymyoglobin-like pigment in pork and that both oxymyoglobin and metmyoglobin developed in beef as a result of irradiation.     

The display of retail packs of ground beef after their storage in master packages under various atmospheres

Batches of coarsely ground beef trimmings were each divided into four portions. One portion from each batch was vacuum packaged, then stored at -1·5°C. The other portions were finely ground and distributed in retail packs. The retail packs were master packaged under atmospheres of N(2), CO(2) or O(2) + CO(2) (2:1, v/v), then stored at 2°C. The product was assessed after storage times of up to 32 days. For each assessment, a vacuum pack and a master pack of each type, each containing products from the same batch, were withdrawn from storage. The vacuum packaged product was finely ground and distributed into retail packs. The newly prepared retail packs and those from the master packs were displayed in a retail cabinet in which air temperatures averaged 4 ± 2°C. The appearance of the displayed product from each storage packaging was assessed twice daily until it was judged to be unacceptable. At the beginning and end of the display, the product in each pack was assessed for discoloration and off-odours, the chemical states of the muscle pigment at the exposed surface were estimated, and the surface microflora was characterized. The appearance of the product displayed after storage in a vacuum pack, for times up to 32 days, became unacceptable within 48 h. A product stored in any of the master packs for 1 day appeared unacceptable after 6 h of display. The display life of products stored under N(2) or CO(2) was similar to that of the vacuum packaged products when storage times were between 2 and 24 days but the display life was shorter when the storage times were 28 or 32 days. The display life of products stored under O(2) + CO(2) was similar to that of the vacuum packaged product when storage times were 2, 4 or 6 days, but the appearance of products stored under O(2) + CO(2) for 8 days or longer was unacceptable when master packs were opened. Apart from those latter packs, a product was not discoloured when storage packs were opened. However, all products were discoloured, and the metmyoglobin fractions of the surface pigments had increased, when the product was withdrawn from display. The products in all storage packagings developed flora dominated by lactic acid bacteria. The spoiage flora on products stored in vacuum pack or under O(2) + CO(2) did not attain the maximum numbers of 10(7)/g during either storage or display. Those maximum numbers were attained on products stored under N(2) and CO(2) after 16 and 28 days storage respectively. Some products stored under N(2) for 16 days or longer developed moderate or strong off-odours during display that were ascribable to microbial action. Other products developed only slight, non-microbial off-odours during display. Retail-ready packs or ground beef master-packaged under an oxygen-depleted atmosphere could then have a useful storage life of about 30 days in commercial circumstances.     

Cholesterol oxidation products in irradiated raw meat with different packaging and storage time

The effect of irradiation and packaging conditions on the formation of cholesterol oxidation products (COPs) as well as lipid oxidation products was determined in raw turkey leg, beef, and pork loin meat during 7 days of storage. Ground turkey leg, beef, and pork loin muscles were prepared as patties. The patties were individually packaged either in oxygen-permeable or impermeable bags, irradiated at 0 or 4.5 kGy using a Linear Accelerator, and stored at 4°C. The COPs such as 7-hydroxycholesterol, 7β-hydroxycholesterol, and 7-ketocholesterol were detected in fresh raw meats at 0 day at the level of 10.9 to 49.2 μg/g lipid. After 7 days of storage, other COPs such as epoxides, 20-hyroxycholesterol, and choletanetriol were formed in mainly aerobically packaged and irradiated raw meats. Packaging effect was more crucial on the cholesterol and lipid oxidation than irradiation. In aerobically packaged and irradiated meats, turkey leg muscles had higher COPs value than beef or pork did. COPs and thiobarbituric acid reactive substances (TBARS) values had a strongly positive correlation in turkey leg and pork. But, cholesterol oxidation in beef proceeded in irradiated and aerobically stored samples despite of its low level of TBARS value.     

Effects of fibre orientation, myoglobin redox form, and postmortem storage on NIR tissue oximeter measurements of beef longissimus muscle

To determine near-infrared tissue oximeter responses to muscle fibre orientation, display time, and surface colour differences of beef longissimus lumborum steaks, beef loins were cut into steaks either perpendicular or parallel to the muscle fibre orientation. Surface colour differences were created by packaging steaks in vacuum (VAC), 80% O₂ and 20% CO₂ modified atmosphere packaging (HiOx MAP), polyvinyl chloride film overwrap (PVC), and HiOx MAP converted to PVC (HiOx-PVC) after 2 days. Changes in surface colour and subsurface pigments during display (0, 2, 4, 10, and 15 days at 2 °C) were characterized by using a reflectance-spectrophotometer and a near-infrared tissue oximeter, respectively. Fibre orientation, storage, and packaging affected (P < 0.05) colour, total pigment, deoxymyoglobin, and oxymyoglobin content. Tissue oximetry measurements appear to have potential for real-time monitoring of myoglobin redox forms and oxygen status of packaged meat, but fibre orientation needs to be controlled.