Photoreceptor cells in the Xenopus retina

This review summarizes our present state of knowledge about spectrally different photoreceptor cell types in the Xenopus retina. The classification of the photoreceptors was based on morphology, combined with immunolabelling with various anti-visual pigment antibodies and other molecular probes on semithin sections and retinal wholemounts. The majority of photoreceptors is represented by rods. Altogether 97-98% of the total rod population consists of the principal ("red") rods that are selectively labeled by N-terminal specific anti-bovine rhodopsin monoclonal antibodies (mAbs) and are maximally sensitive to green light. The other, rare, blue-sensitive rod type ("green rod") is thinner, not stained by these antibodies but binds C-terminal specific anti-rhodopsin mAbs. The major representatives of the cones are red-sensitive and consist of a morphologically heterogeneous group comprising both (principal and accessory) members of double cones, as well as large single cones. Outer segments in this group are selectively labeled by mAb COS-1, specific to the L/M group of cone visual pigments. Another, relatively rare cone type is similar in size, but slightly smaller than the large single cone and is not labeled by mAb COS-1. This cone type is assumed to have a blue-sensitive cone visual pigment. The third, least abundant, and immunocytochemically distinct cone type is a small single (miniature) cone, which binds mAb OS-2 relatively strongly, and anti-rhodopsin mAbs 4B4 and 1D4 weakly. By exclusion, this small single cone may be identical with the UV-sensitive cone. Further studies are needed, however, to identify the color sensitivity of the latter two cone types.     

The complexity of the visual cells and visual pathways of the sturgeon

The visual cells in the retinae of the sturgeon were studied by scanning electron microscopy and transmission electron microscopy. Our investigations revealed the presence of rods, two types of single cones, one type of double cone (two nonidentical cone components adhered together), and one type of twin cone (two identical cone components adhered together). In some of the cones, large glycogen bodies were present in the inner segments and all cones contained oil droplets. Such cone morphology was very similar to that described in the retinae of higher vertebrates, for example the chicken. DiI tracing of retinofugal pathways following uniocular injection demonstrated their bilateral localization and extensive termination in the diencephalon and mesencephalon of both sides. Fibers also crossed over from one side to another through commissures, including the posterior commissure. The complexity of the pathway surpassed that of the teleosts and further indicated the evolutionary importance of this fish.     

Morphogenesis of the different types of photoreceptors of the chicken (Gallus domesticus) retina and the effect of amblyopia in neonatal chicken

Despite the great variety in chicken photoreceptors, existing morphogenetic studies only deal with two types: rods and cones. We have therefore examined by scanning electron microscopy the first appearance and maturation of different retinal photoreceptors in 36 chicken embryos (Gallus domesticus), aged 5-19 days prehatching. On day 5 of incubation, chicken retinae were only composed of proliferating ventricular cells devoid of photoreceptors. On day 8, outer mitotic cells were separated from inner differentiating photoreceptors, by the transient layer of Chievitz. Ball-like protrusions appeared at the ventricular surface, representing the first signs of photoreceptor inner segment formation. From day 10 onward, double cones, single cones, and rods could be clearly distinguished, and occasional cilia were detected at their tip. On day 12, inner segments had increased in length and diameter, and frequently carried a cilium representing the beginning of outer segment formation. On day 14, most photoreceptors displayed a distinct outer segment. On day 19, photoreceptors had essentially assumed adult morphology. Based on the shape of their outer segments, two subtypes of cones and three subtypes of double cones could be distinguished. Throughout development, we observed microvilli close to maturing photoreceptors, either originating from their lateral sides, from their tip, or from Müller cells. Microvillus density peaked between day 12 and 14, indicating an important role in photoreceptor morphogenesis. Unilateral occlusion of the eyes of posthatching chicken reduced the proportion of double cones to single cones in the retina, indicating dependence of retinal morphogenesis upon functional activity of visual cells.     

Mesopic state: cellular mechanisms involved in pre- and post-synaptic mixing of rod and cone signals

At least twice daily our retinas move between a light adapted, cone-dominated (photopic) state and a dark-adapted, color-blind and highly light-sensitive rod-dominated (scotopic) state. In between is a rather ill-defined transitional state called the mesopic state in which retinal circuits express both rod and cone signals. The mesopic state is characterized by its dynamic and fluid nature: the rod and cone signals flowing through retinal networks are continually changing. Consequently, in the mesopic state the retinal output to the brain contained in the firing patterns of the ganglion cells consists of information derived from both rod and cone signals. Morphology, physiology, and psychophysics all contributed to an understanding that the two systems are not independent but interact extensively via both pooling and mutual inhibition. This review lays down a rationale for such rod-cone interactions in the vertebrate retinas. It suggests that the important functional role of rod-cone interactions is that they shorten the duration of the mesopic state. As a result, the retina is maintained in either in the (rod-dominated) high sensitivity photon counting mode or in the second mode, which emphasizes temporal transients and spatial resolution (the cone-dominated photopic state). Experimental evidence for pre- and postsynaptic mixing of rod and cone signals in the retina of the clawed frog, Xenopus, is shown together with the preeminent neuromodulatory role of both light and dopamine in controlling interactions between rod and cone signals. Dopamine is shown to be both necessary and sufficient to mediate light adaptation in the amphibian retina.     

旋风车削锥形连接钎杆锥体的改进

本文详尽介绍了锥形连接钎杆锥体加工的一种新方式—旋风车削。此种旋风车削是针对锥体加工的要求而对旋风车床进行改造后形成的不同于通常旋风车削方式的新加工方式。其特点是加工时工件不旋转,相较于其它的锥体加工方式这种旋风车削具有更高的效率和很好的安全性。     

Maximum diameter of the rod-shaped specimen for transmission electron microtomography without the "missing wedge"

In our recent study, the complete rotation of a rod-shaped specimen during transmission electron microscopy (TEM) has been successfully carried out, yielding a truly quantitative three-dimensional (3D) structure of a ZrO(2)/polymer nano-composite. This result allows the further development of transmission electron microtomography (TEMT) for materials science. The diameter of the rod-shaped specimen was about 150 nm, which may not be statistically large enough to evaluate structural parameters, e.g., volume fraction of Zr nano-particles. Thus, it is preferable to image rods with larger diameters in 3D. In this study, several rod-shaped specimens whose diameters ranged from 150 to 530 nm were subjected to the "distortion-free TEMT". The maximum diameters, l, observable under 200 and 300 kV-TEMTs were, respectively, 460-470 and 600-670 nm (corresponding the maximum relative diameters, l/lambda (lambda: mean free path), were ca. 2.2 and 2.7-3.0).     

Distribution of GABA and glycine receptors on bipolar and ganglion cells in the mammalian retina

The amino acids GABA and glycine mediate synaptic transmission via specific neurotransmitter receptors. Molecular cloning studies have shown that there is a great diversity of GABA and glycine receptors. In the present article, the distribution of GABA and glycine receptors on identified bipolar and ganglion cell types in the mammalian retina is reviewed. Immunofluorescence obtained with antibodies against GABA and glycine receptors is punctate. Electron microscopy shows that the puncta represent a cluster of receptors at synaptic sites. Bipolar cell types were identified with immunohistochemical markers. Double immunofluorescence with subunit-specific antibodies was used to analyze the distribution of receptor clusters on bipolar axon terminals. The OFF cone bipolar cells seem to be dominated by glycinergic input, whereas the ON cone bipolar and rod bipolar cells are dominated by GABAergic input. Ganglion cells were intracellularly injected with Neurobiotin, visualized with Streptavidin coupled to FITC, and subsequently stained with subunit specific antibodies. The distribution and density of receptor clusters containing the alpha1 subunit of the GABA(A) receptor and the alpha1 subunit of the glycine receptor, respectively, were analyzed on midget and parasol cells in the marmoset (a New World monkey). Both GABA(A) and glycine receptors are distributed uniformly along the dendrites of ON and OFF types of parasol and midget ganglion cells, indicating that functional differences between these subtypes of ganglion cells are not determined by GABA or glycinergic input.     

直母线锥盘金属带式无级变速器带的轴向偏移分析

分析了直母线锥盘金属带式无级变速器带的轴向偏移。阐述了传统控制方法的弊端：传统曲母线锥盘与金属片产生角点接触，在传动比i=1附近传动比变化不连续；在传动比i=1时，预置带的轴向偏移量只能使带的最大偏移量减小到未调整时的50％～60％。提出复合母线锥盘，锥盘母线与金属片侧边共轭，既完全消除了金属带的轴向偏移，又与直母线锥盘具有相同的接触强度，提高了无级变速器的传动能力，扩大了无级变速器的应用范围。     

Transfer Solid Lubrication of Aluminum Sliding Contacts

The effects of transfer from solid lubricant sticks of unfilled, glass-filled, and bronze-filled PTFE on the room-temperature wear and friction of trailing primary contacts of aluminum (6061 T6) rods in repetitive intermittent contacts were investigated in a ring-on-rod configuration. The materials of the ring countersurfaces upon which the solid lubricants transferred and against which the trailing aluminum rods wore included steel, aluminum, copper, and an oxide dispersion-strengthened copper alloy. This sliding of the unlubricated copper ring countersurfaces against the aluminum led to the roughening of the copper as large (> 1 mm) aluminum particles embedded themselves upon the countersurface, with consequent transitions in the aluminum wear rate and the coefficient of friction to values exceeding 6 × 10^{? 3} mm³/Nm and 0.6, respectively, after an incubation period of several initial contacts of lower wear rate and friction. The other ring countersurface materials resulted in similarly high aluminum rod wear rate and coefficient of friction, more nearly from the onset of sliding. The application of unfilled PTFE solid lubricant transfer reduced the aluminum's gouging of the copper countersurfaces and correspondingly reduced the aluminum rod wear rate and the coefficient of friction against the copper, as well as against all other countersurface materials, towards 2 × 10^?3 mm³/Nm and 0.3 or less, respectively. Glass- and bronze-filled PTFE transfer lubricants provided reductions in the wear rate of the aluminum rod comparable to or in some cases better than the unfilled PTFE, though the unfilled PTFE transfer lubricant in several cases provided better friction reduction.     

A possible explanation for the hydrodynamic transition in high speed impact

The velocity at which the mode of penetration of a rod striking a thick target changes from gross plastic deformation to a jetting type of flow has been called the hydrodynamic transition velocity. It is proposed that this velocity depends on the relative rates of rod erosion and plastic wave propagation. A simple theory predicts the hydrodynamic transition velocity for right cylindrical rods striking normally a thick target in terms of the empirical dynamic strength of the rod and target and the dynamic work hardening rate or dynamic large strain tangent modulus of the rod material.     

Mean free paths for inelastic electron scattering in silicon and poly(styrene) nanospheres

The mean free paths for inelastic electron scattering, lambda(in), in silicon [Si] and poly(styrene) [PS] have been measured using off-axis electron holography in a field-emission transmission electron microscope (FEG TEM). The holographic imaging method determines both quantitative wave phase information as well as elastic energy-filtered wave amplitude information. Using the energy-filtered amplitude data, two-dimensional t/lambda(in) images are reconstructed. The present work uses spherical nanoparticles as samples, so the sample thickness at any point in a two-dimensional image can be calculated knowing the center and radius of the projected nanosphere. The thickness contribution to t/lambda(in) is removed to obtain quantitative lambda(in) values. This work finds values of lambda(i)Si = 53.8 +/- 5.5 and 88.6 +/- 6.9 nm, and lambda(PS) = 78.1 +/- 3.4 and 113.0 +/- 5.9 nm for 120 and 200 keV incident electron energies, respectively.     

Instability and self-contact phenomena in the writhing of clamped rods

We use the Cosserat rod theory to present a unified picture of jump phenomena, associated with looping, snap-through, pop-out, etc., in twisted clamped rods undergoing large deflections. Both contact-free rods and rods with isolated points of self-contact are considered. Taking proper account of the symmetries of the problem we find that an arbitrary contact-free solution is fully characterised by four parameters; each point contact adds another two. A shooting method is used for solving the boundary value problem. An intricate bifurcation picture emerges with a strong interplay between planar and spatial rod configurations. We find new jump phenomena by treating the ratio of torsional to bending stiffness of the rod as a bifurcation parameter. Load-deflection curves are computed and compared with results from carefully conducted experiments on contact-free as well as self-contacting metal-alloy rods.     

Electron microscopy of melamine-embedded frog retina: evidence for the overall crystalline organization of photoreceptor outer segments

Melamine-formaldehyde, a widely used technical aminoplast, was recently introduced as a water-soluble embedding-medium for electron microscopy (Bachhuber & Frösch, 1983). When processed, using this new technique, the membranes and associated cytoplasmic structures of rod and cone discs show a high degree of structural organization. The membranes are symmetrical and composed of arrays of a 2–2·5 nm particle with a very regular spacing of 3 nm. A network of delicate filaments of less than 2 nm is superimposed on to this framework of membranes, running perpendicularly between the discs and across the disc membranes. The outer segment as a whole shows a crystal-like organization.     

A source of a reference spherical wave based on a single mode optical fiber with a narrowed exit aperture

A new type of a reference spherical wave source (SWS) based on a single mode optical fiber with a narrowed down up to the submicrometer size exit aperture is proposed. It is intended for the precision point diffraction interferometers as a source of a reference wave. Systematic experimental errors which influence the measurement accuracy of the quality of the wave fronts generated by the SWSs are considered. Experimental data on wave front deformations are given. The combined root-mean-square (rms) wave deformation for a couple of the SWSs measured in a numerical aperture of NA=0.27 reaches the value of rms=0.36 nm that corresponds to rms=0.25 nm of a single SWS or about lambda2500 for the red He-Ne laser.     

三醋酸甘油酯自动喷雾系统的应用

AF2/KDF2型滤棒成型机组的增塑剂上胶系统,采用毛刷旋转甩涂三醋酸甘油酯控制滤棒硬度,存在三醋酸甘油酯喷涂不均匀、丝束与三醋酸甘油酯浪费严重等问题,为提高滤棒质量,进行了基于雾化技术的三醋酸甘油酯自动喷雾系统的改造.改造后的系统靠管腔内、外形成的压力差,由喷嘴喷出雾化良好的甘油,均匀附着在丝束上,克服了原上胶方式中控制过程的非线性和时滞性缺点,测试结果表明,提高了咀棒质量,降低了废棒率,也相应降低了甘油用量.     

Ultrastructural aspects of peptidergic modulation in the peripheral nervous system of Helix pomatia

The ultrastructural characteristics of peptidergic peripheral contacts in the snail, Helix pomatia, were investigated, with special attention to the innervation of the heart, buccal mass, and salivary gland by Mytilus inhibitory peptide-immunoreactive neurons. Following the application of correlative light- and electron-microscopic pre-embedding immunocytochemistry, the peripheral tissues reveal a rich innervation by Mytilus inhibitory peptide-immunoreactive elements. These neurons establish three types of neuromuscular contacts in the heart and buccal mass: (1) close (16-20 nm) unspecialized membrane contacts; (2) contacts with a relative wide (40-100 nm) intersynaptic cleft; and (3) labeled varicosties located freely in the extracellular space, far (0. 5-several microm) from the muscle cells. In the salivary gland, the immunoractive profiles contact both the muscular and glandular elements with close (type 1) and wider (type 2) membrane attachments. The great majority of Mytilus inhibitory peptide-immunoreactive profiles contain an ultrastructurally uniform population of large (120-150 nm) electron dense granules. The ultrastructural features of the innervation by Mytilus inhibitory peptide-immunoreactive elements are compared with those established by immunogold labelled FMRFamide-containing profiles in the heart and salivary gland. These latter display similarities in forming the different kinds of intercellular contacts, and differences in the morphological variability of the content of granules in the immunolabeled profiles. The results suggest diverse, non-synaptic modulatory roles of neuropeptides in the peripheral nervous system of Helix pomatia, including localized membrane effects and neurohormonal-like remote global controls, that may also be of significance in orchestrating the effects of neuropeptides released at the same time on different targets.     

Transmission Electron Microscopy of Boundary-Lubricated Bearing Surfaces. Part I: Mineral Oil Lubricant

Transmission electron microscopy (TEM) was performed on the near-surface material (depth <500 nm) of tapered roller bearing inner rings (cones) that were tested at two levels of boundary-lubricated conditions in mineral oil with no additives. Site-specific thinning of cross section cone surface sections for TEM analyses was conducted using the focused ion beam (FIB) milling technique. High-resolution structural and compositional characterization of near-surface material and surface layers was performed on an untested cone as well as cones tested at Λ～1.1 and 0.3. This approach revealed near-surface microstructural distortion and grain size gradients that were attributed to surface finishing operations during manufacture. The characteristics of oxide surface layers and micro-cracks on the tested bearing surfaces were evaluated and found to depend on lubrication conditions.     

Inducing superconductivity at a nanoscale: photodoping with a near-field scanning optical microscope

The local modification of an insulating GdBa2Cu3O6.5 thin film, made superconducting by illumination with a near-field scanning optical microscope (NSOM), is reported. A 100-nm aperture NSOM probe acts as a sub-wavelength light source of wavelength lambda(exc) = 480-650 nm, locally generating photocarriers in an otherwise insulating GdBa2-Cu3O6.5 thin film. Of the photogenerated electron-hole pairs, electrons are trapped in the crystallographic lattice, defining an electrostatic confining potential to enable the holes to move. Reflectance measurements at lambda = 1.55 microm at room temperature show that photocarriers can be induced and constrained to move on a approximately 200 nm scale for all investigated lambda(exc). Photogenerated wires present a superconducting critical temperature Tc= 12 K with a critical current density Jc = 10(4) A cm(-2). Exploiting the flexibility provided by photodoping through a NSOM probe, a junction was written by photodoping a wire with a narrow (approximately 50 nm) under-illuminated gap. The strong magnetic field modulation of the critical current provides a clear signature of the existence of a Josephson effect in the junction.     

Comparative ultrastructure and cytochemistry of the avian pineal organ

The breeding of birds is expected to solve problems of nourishment for the growing human population. The function of the pineal organ synchronizing sexual activity and environmental light periods is important for successful reproduction. Comparative morphology of the avian pineal completes data furnished by experiments on some frequently used laboratory animals about the functional organization of the organ. According to comparative histological data, the pineal of vertebrates is originally a double organ (the "third" and the "fourth eye"). One of them often lies extracranially, perceiving direct solar radiation, and the other, located intracranially, is supposed to measure diffuse brightness of the environment. Birds have only a single pineal, presumably originating from the intracranial pineal of lower vertebrates. Developing from the epithalamus, the avian pineal organ histologically seems not to be a simple gland ("pineal gland") but a complex part of the brain composed of various pinealocytes and neurons that are embedded in an ependymal/glial network. In contrast to organs of "directional view" that develop large photoreceptor outer segments (retina, parietal pineal eye of reptiles) in order to decode two-dimensional images of the environment, the "densitometer"-like pineal organ seems to increase their photoreceptor membrane content by multiplying the number of photoreceptor perikarya and developing follicle-like foldings of its wall during evolution ("folded retina"). Photoreceptor membranes of avian pinealocytes can be stained by antibodies against various photoreceptor-specific compounds, among others, opsins, including pineal opsins. Photoreceptors immunoreacting with antibodies to chicken pinopsin were also found in the reptilian pineal organ. Similar to cones and rods representing the first neurons of the retina in the lateral eye, pinealocytes of birds possess an axonal effector process which terminates on the vascular surface of the organ as a neurohormonal ending, or forms ribbon-containing synapses on pineal neurons. Serotonin is detectable immunocytochemically on the granular vesicles accumulated in neurohormonal terminals. Pinealocytic perikarya and axon terminals also bind immunocytochemically recognizable excitatory amino acids. Peripheral autonomic fibers entering the pineal organ through its meningeal cover terminate near blood vessels. Being vasomotor fibers, they presumably regulate the blood supply of the pineal tissue according to the different levels of light-dependent pineal cell activity.