Purealin blocks the sliding movement of sea urchin flagellar axonemes by selective inhibition of half the ATPase activity of axonemal dyneins

Ciliary and flagellar movements are explained by active sliding between the outer doublet microtubules of an axoneme via their inner and outer dynein arms. Purealin, a novel bioactive principle of a sea sponge Psammaplysilla purea, blocked the motility of Triton-demembranated sea urchin sperm flagella within 5 min at concentrations above 20 microM. In a similar concentration range, purealin blocked the sliding movement of the flagellar axonemes in vitro within a few minutes judging from the turbidity measurements. The ATPase activity of axonemes was partially inhibited by purealin in a concentration-dependent manner. The maximum inhibition reached approximately 50% at concentrations above 20 microM, indicating that half the axonemal ATPase activity is sensitive to purealin. Similar results were observed on the ATPase activity of outer-arm-depleted axonemes and that of a mixture of 21S dynein and salt-extracted axonemes. On the other hand, ATPase activity of isolated 21S dynein was not inhibited by purealin. The inhibitory action of purealin on the axonemal ATPases was reversed by dilution of purealin. The effect of purealin on the double-reciprocal plot of the ATPase activity as a function of ATP concentrations showed that the inhibition was not a competitive type. In accord with this finding, purealin did not affect the vanadate-mediated UV photocleavage of axonemal dyneins. These results suggest that purealin binds reversibly to a site other than the catalytic ATP-binding site and inhibits half the ATPase activity of axonemes. Taken together, our results suggest that purealin-sensitive ATPase activity of the dynein arms plays an essential role in generating the sliding movement of flagellar axonemes.     

The chromatin structure of well-spread demembranated human sperm nuclei revealed by atomic force microscopy

The fundamental structure formed when genomic DNA is packaged by protamine in the human sperm nucleus still remains essentially unresolved. It is known that the binding of protamine, a small arginine-rich protein, to DNA generates a large dense, hydrophobic complex making the sperm chromatin structure difficult to study microscopically. To visualize the internal nuclear structures, isolated human sperm nuclei were swollen extensively in saline buffer using only a reducing agent. The nuclei were swollen during deposition onto coverglass and then imaged in the atomic force microscope (AFM). The two main results obtained from imaging individual well-spread nuclei indicate that native human sperm chromatin is: (1) particulate, consisting primarily of large nodular structures averaging 98 nm in diameter, and (2) also composed of smaller, nucleosome-like particles observed to form linear chains near the nuclear periphery. These two types of chromatin particles imaged by AFM are remarkably similar to other AFM measurements made on native and reconstituted sperm and somatic chromatin.     

Comparative study of the specific stiffness and yield of powder compact and porous materials during bend testing

Comparative analysis of the mechanical behavior of compact and porous beams operating under a bending regime is described. Experimental procedures are suggested for determining the different normalized parameters that characterize the mechanical behavior of porous and compact beams. The effect of pore space structure characterized by parameters m (the ratio of pore-former material and powder particle sizes) and ? (pore volume fraction) on the characteristics studied in this work for highly porous materials prepared by introducing a pore-former is analyzed.     

The fine structure of the sperm of a holothurian and an ophiuroid

The fine structure of the mature sperm of the holothurian, Cucumaria miniata, and the ophiuroid, Ophiopholis aculeata, is described with particular reference to their acrosomal and centriolar satellite complexes, and compared to the sperm of other echinoderms. In Cucumaria, the acrosome is in the form of a diffuse acrosomal vesicle. It is unusual in that it apparently lacks an acrosomal membrane. A membrane separating the acrosomal vesicle from the periacrosomal material may not be equivalent to a typical inner acrosomal membrane. In Ophiopholis, the acrosome is dense, with some internal substructure, and is enclosed by a complete acrosomal membrane. In both species, the acrosome is partially surrounded by an amorphous periacrosomal mass. There is a notable absence of a subacrosomal depression and associated structures as found in other echinoderm sperm. The centriolar satellite complex (CSC) is essentially identical in both species. A reconstruction of the CSC is presented. The CSC consists of nine satellites radiating angularly from the distal centriole, each bifurcating at a dense node before inserting on a marginal ring containing circumferential microtubules. The ring is probably a cytoskeletal element. Immediately below the satellites are nine Y-shaped connectives, connecting each of the axonemal alpha doublets to the flagellar membrane.     

Dry sliding wear response of some bearing alloys as influenced by the nature of microconstituents and sliding conditions

An attempt has been made in this study to examine the dry sliding wear response of a leaded-tin bronze, an aluminum bronze, and a conventional zinc-based alloy under varying applied pressure and speed conditions. Different characteristics of the microconstituents of the alloys have been correlated with that of their wear behavior. The study clearly indicates that the influence of the microstructural features greatly changes with the sliding conditions. It also has been observed that in order to attain good wear characteristics, a material should comprise an optimum level of lubricating, load bearing and ductile microconstituents, and, above all, thermal stability. Room temperature properties in fact play rather a secondary role in this context.     

Dry sliding wear response of some bearing alloys as influenced by the nature of microconstituents and sliding conditions

An attempt has been made in this study to examine the dry sliding wear response of a leaded-tin bronze, an aluminum bronze, and a conventional zinc-based alloy under varying applied pressure and speed conditions. Different characteristics of the microconstituents of the alloys have been correlated with that of their wear behavior. The study clearly indicates that the influence of the microstructural features greatly changes with the sliding conditions. It also has been observed that in order to attain good wear characteristics, a material should comprise an optimum level of lubricating, load bearing and ductile microconstituents, and, above all, thermal stability. Room temperature properties in fact play rather a secondary role in this context.     

The inter-relationship between grain boundary sliding and cavitation during creep of polycrystalline copper

High-temperature creep tests were conducted on polycrystalline copper of commercial purity in order to investigate the inter-relationship between the extent and the rate of grain boundary sliding (GBS) and the development of internal cavitation. An image processing technique was used to provide quantitative information on the size and shape of the cavities formed over a range of stresses and at temperatures from 673 to 873 K. The results demonstrate that cavity development is inhomogeneous within any specimen, such that there is an increase in the level of cavitation in regions of higher local strain. It is shown by quantitative measurements that the total cavitated area increases at the faster sliding rates, but the number density of cavities decreases at the highest sliding rates because of cavity interlinkage. When interlinkage is taken into account, the results confirm that the overall level of cavitation is related to the occurrence of GBS. On leave from the Department of Physics, University of Cape Coast, Cape Coast, Ghana This article is based on a presentation made at the “High Temperature Fracture Mechanisms in Advanced Materials” sympsosium as a part of the 1994 Fall meeting of TMS, October 2-6, 1994, in Rosemont, Illinois, under the auspices of the ASM/SMD Flow and Fracture Committee.     

Crack initiation and propagation during high-temperature fatigue of oxide dispersion-strengthened superalloys

The mechanisms of crack initiation and propagation have been investigated in two oxide dispersion-strengthened (ODS) Ni-base superalloys under conditions of symmetric low-cycle fatigue (LCF) and creep-fatigue. The behavior of both ODS alloys is compared with that of conventional alloys of otherwise similar composition. While the improvement in fatigue resistance previously reported for ODS metals and alloys is confirmed by the present study for temperatures below about0.6T _m, the potential advantage of dispersion strengthening is not being exploited by the current generation of ODS superalloys at higher temperatures; crack initiation is found to occur prematurely due to the presence of recrystallization defects in the form of fine grains. The mechanism of crack initiation at fine grains is creep-type cavitation on boundaries transverse to the applied stress. Experimental results indicating the influence of temperature, loading frequency, and waveshape on the crack initiation rate are presented and discussed in detail. A qualitative correlation between waveshape and creep-fatigue life is suggested based on the macroscopic inelastic strain rate which is determined by the waveform and limits in turn the rate at which cavity growth can be accommodated. Formerly with Max-Planck-Institut für Metallforschung     

Repair of mitral valve prolapse by resection and sliding plasty

There have been many techniques applied to the repair of mitral valve prolapse, and the method used in a particular case is usually selected according to the position and extent of the lesion. To simplify and standardize the technique of mitral valve repair, we have adopted the resection, sliding plasty and ring annuloplasty methods since December 1992. Of 10 consecutive surgical cases, 2 involved prolapse of the anterior leaflet, 1 the posteromedial commissural, and 7 the posterior leaflet. One patient with posterior leaflet prolapse required valve replacement due to dehiscence of the plastied site on the 3rd postoperative day, and one died because of sepsis. However, the remaining patients were doing well without mitral regurgitation at a mean of 20 months (range: 8-32) after the operation. The advantages of these techniques include easy adjustment of the height of the leaflet and a good chance of long-term durability, since the affected lesion is resected.     

Topographic analysis of coherence and propagation of EEG activity during sleep and wakefulness

Overnight sleep EEG recorded from 21 derivations was studied in 8 healthy subjects. The vector autoregressive model was fitted to all 21 channels simultaneously. Ordinary, multiple and partial coherences and directed transfer functions were estimated for sleep stages and wakefulness. Ordinary coherences give rather trivial information that coherence decreases with distance. Partial coherences revealed specific structure that was well repeatable for the subjects studied. Differences in coherence patterns between sleep stages were found by means of statistical tests. An increase of coherence was found for sleep stages 2, 3 and 4. Directed transfer function made possible the identification of the main centers from which EEG activity is spreading during sleep and wakefulness. During sleep the influence of subcortical structures was manifested by propagation of activity from the fronto-central region. The range of this interaction was highest in sleep stages 3 and 4. An EEG analysis, based on the approach of treating time series as a realization of one process and on the simultaneous (not pair-wise) evaluation of signals offers new possibilities in the investigation of synchronization and functional relations in the brain.     

连铸粘结漏钢的结晶器温度变化及其传播行为

以国内某钢厂板坯连铸粘结漏钢的实测样本为基础,重点调查了铸坯粘结时结晶器热电偶温度变化,分析了粘结传播行为,讨论了现行热电偶布置合理性和漏钢预报模型的设计.研究结果表明:粘结时单个热电偶温度在时间上表现先升后降变化规律,且具有一定温度变化速率和持续时间,多行多列相邻热电偶温度变化在空间上表现粘结传播的"时滞性",具有典型性,同列热电偶温度变化表现"温度倒置"现象,但不具典型性;粘结纵向传播速度小于拉速,与拉速呈线性正相关,横向传播速度与拉速比为 0.91 ~ 1.91,存在较大不稳定性;从纵横向检测快慢和稳定性综合考虑,以纵向检测为主、横向检测为辅设计漏钢预报模型更为合适;现行热电偶布置较为合理.     

Effect of sliding and rolling friction on the energy-force parameters during hot rolling in four-high stands

A procedure for the calculation of the main-drive power for a hot-rolling wide-strip mill is developed. It takes into account that 85–99% of the lengths of the deformation zones in the working stands of such mills are occupied by stick zones, in which a strip undergoes static friction stresses (which do useful work only in the backward slip zone). This procedure also takes into account the rolling friction energy losses, which account for 83–93% of the power consumed for the rotation of idle backup rolls or 29–68% of the total energy consumed by the main mill drive. The average power calculation error for this procedure is 5%, and the maximum error is 10%, which is three to five times smaller than the errors of well-known calculation procedures. Our procedure has a high potential for revealing the reserves of decreasing the contact stresses in rolls and the saving of electric power via the redistribution of the reductions and tensions between stands and an increase in the temperature of the semi-finished rolled products.     

Interface sliding,migration, and cracking during fatigue deformation of a superplastic aluminum-zinc eutectoid alloy

A superplastic aluminum-zinc eutectoid alloy was fatigue tested at 100 °C and 200 °C at different constant plastic strain amplitudes and strain rates. During fatigue deformation, the average peak stress increased with increasing strain rate and grain size and decreasing temperature. It was almost independent of the plastic strain amplitude. To detect interfacial sliding, interphase boundary migration, and intergranular cracking, selected areas on surfaces were examined before fatigue deformation and re-examined after fatigue deformation. Interface sliding, which was almost reversible, occurred on (Al)/(Al) and (Zn)/(Zn) grain boundaries and on (Al)/(Zn) interphase boundaries. Grains appeared to slide in groups. Cracks followed grain and interphase boundaries. Along an intergranular crack, most interfaces were (Zn)/(Zn) grain boundaries and (Al)/ (Zn) interphase boundaries. Grains deformed to accommodate interfacial sliding. The absence of slip lines suggested that diffusional creep made a significant contribution to deformation in grains of the zinc-rich phase. Deformation of the aluminum-rich phase involved the glide and climb of dislocations. J. W. BOWDEN, formerly Graduate Student, Department of Metallurgy and Materials Science, University of Toronto.     

Crack propagation of steels during low cycle thermal-mechanical and isothermal fatigue at elevated temperatures

The surface and through crack propagation of three high temperature steels in low cycle thermal-mechanical and isothermal fatigue at elevated temperatures was investigated. The rate of crack propagation obtained was correlated with the range of cyclicJ-integral, ΔJf. It was found that there is a linear relationship on alog-log plot regardless of materials, test conditions, and crack configurations. Furthermore, fatigue life predicted by integrating the equation of crack propagation was compared with experimental results.     

Expression and possible role of muscle-type carnitine palmitoyltransferase I during sperm development in the rat

Because we had found whole testis from adult rats to be much richer in the messenger RNA for the muscle (M) than for the liver (L) form of mitochondrial carnitine palmitoyltransferase I (CPT I), we sought to determine which cell type(s) accounts for this expression pattern and how it might relate to reproductive function. Studies with immature (14-day-old) and adult animals included 1) Northern blot analysis of testis mRNA; 2) in situ hybridization with slices of testis; 3) enzyme assays for CPT I, CPT II, and carnitine acetyltransferase (CAT) in testicular germ cells and nongerm cells, together with measurement of the malonyl-coenzyme A (CoA) sensitivity and affinity for carnitine of CPT I; 4) labeling of testicular CPT I with [3H]etomoxir, a covalent inhibitor of the enzyme; and 5) the response of testicular and nontesticular CPT I to dietary etomoxir. The data established the following: 1) L-CPT I was the sole isoform detected in immature testis. 2) Expression of the M-CPT I gene was associated only with meiotic and postmeiotic germ cells. 3) Adult testis contains a mixture of the L- and M-CPT I enzymes, the L and M form dominating in extratubular cells and spermatids, respectively. Mature epididymal spermatozoa appear to be devoid of CPT I activity while possessing abundant levels of CPT II and CAT. 4) Five days of dietary etomoxir treatment at a dose that resulted in essentially complete inhibition of CPT I in liver, heart, skeletal muscle, and kidney was totally without effect on either the L- or M-type enzyme in the testis of mature rats. The data point to an important role for transient expression of M-CPT I, coupled with sustained activity of CAT, in the maturation and/or function of rat sperm. They also suggest that, at least in the case of one CPT I inhibitor (etomoxir), the testis is unusually resistant to the agent when given orally.     

Distinct roles for the actin and microtubule cytoskeletons in the morphogenesis of epidermal hairs during wing development in Drosophila

We have found that the actin and microtubule cytoskeletons have overlapping, but distinct roles in the morphogenesis of epidermal hairs during Drosophila wing development. The function of both the actin and microtubule cytoskeletons appears to be required for the growth of wing hairs, as treatment of cultured pupal wings with either cytochalasin D or vinblastine was able to slow prehair extension. At higher doses a complete blockage of hair development was seen. The microtubule cytoskeleton is also required for localizing prehair initiation to the distalmost part of the cell. Disruption of the microtubule cytoskeleton resulted in the development of multiple prehairs along the apical cell periphery. The multiple hair cells were a phenocopy of mutations in the inturned group of tissue polarity genes, which are downstream targets of the frizzled signaling/signal transduction pathway. The actin cytoskeleton also plays a role in maintaining prehair integrity during prehair development as treatment of pupal wings with cytochalasin D, which inhibits actin polymerization, led to branched prehairs. This is a phenocopy of mutations in crinkled, and suggests mutations that cause branched hairs will be in genes that encode products that interact with the actin cytoskeleton.