Escherichia coli O157:H7 in drinking water from private water supplies in the Netherlands

The microbiological quality of drinking water from 144 private water supplies in the Netherlands was tested and additionally the occurrence of Escherichia coli O157 was examined. Faecal indicators were enumerated by using standard membrane filtration methods. The presence of E. coli O157 was determined using a specific enrichment method. Eleven percent of the samples contained faecal indicators whereas E. coli O157:H7 was isolated from 2.7% of the samples that otherwise met the drinking water standards. The E. coli O157 positive water supplies were located on camp-sites in agricultural areas with large grazer densities. Pulsed field gel electrophoresis (PFGE) analysis suggested that cattle might have been the cause of contamination. Our results indicate that compliance with microbiological quality standards obtained in routine monitoring does not always guarantee the absence of pathogens. The presence of pathogens such as E. coli O157 may suggest possible health consequences; however, a risk assessment process should be performed as the monitoring of both faecal indicator parameters and pathogens do not predict the effect of microbial contamination of drinking water on a population.     

Risk factors contributing to microbiological contamination of shallow groundwater in Kampala,Uganda

A study of water quality variation in shallow protected springs in Kampala was undertaken over a 12-month period to assess the causes of microbiological contamination. The microbiological quality of water was assessed using thermotolerant coliforms and faecal streptococci. Sanitary inspections and hazard assessments were undertaken to identify faecal sources (hazards), contaminant pathways and contributory factors. Data were collected on rainfall and population as additional factors potentially exerting an influence on microbiological quality. Initial analysis of the data showed a significant relationship between median level of contamination and rainfall, in particular to short-term rainfall events. Total sanitary risk score showed a significant relationship with median level of contamination, but population density may be a confounding factor. The raw microbiological data were transformed into five water quality targets: <1 and < or =10 cfu 100ml(-1) for faecal streptococci; and <1, < or =10 and < or=50 cfu 100 ml(-1) for thermotolerant coliforms. The presence of individual risk factors as well as variables for rainfall and population density were analysed with respect to failure to meet these water quality targets using contingency tables. Logistic regression models were developed for each of the five water quality targets. The analysis strongly suggested that there is rapid recharge of the springs after rainfall and this leads to microbiological contamination. On-site sanitation was less important than other sources of faecal matter, which was consistent with a low sanitation coverage in the study area. The study suggested that improving sanitary completion and local environmental hygiene was more important than controlling on-site sanitation in improving the quality of these springs.     

Arsenic-contaminated cold-spring water in mountainous areas of Hui County, Northwest China: a new source of arsenic exposure

Although pump-well is the primary drinking water source in rural areas of China, there are still 8.4% of villages reliant on cold-spring. In this study, a survey of arsenic concentration in cold-springs and pump-wells was carried out in Hui County, Northwest China. A total of 352 drinking water samples, including 177 cold-springs and 175 pump-wells, were collected. The maximum arsenic concentrations in cold-springs and pump-wells were 0.482 mg/L and 0.067 mg/L, respectively. We found that 15.8% (28) of total cold-springs and 1.1% (2) of total pump-wells had arsenic concentrations exceeding the maximum allowable concentration of arsenic in drinking water of rural China (0.05 mg/L). Our findings show that 5 cold spring-contaminated villages are located in the mountainous areas of Hui County and 2224 inhabitants may be at risk of high arsenic exposure. This paper indicates that arsenic contamination of cold-springs may be more serious than expected in mountainous areas of Northwest China and extensive surveys and epidemiological studies should be carried out to investigate the potential contaminated areas and affected population.     

Rapid, cultivation-independent assessment of microbial viability in drinking water

Fast and accurate monitoring of chemical and microbiological parameters in drinking water is essential to safeguard the consumer and to improve the understanding of treatment and distribution systems. However, most water utilities and drinking water guidelines still rely solely on time-requiring heterotrophic plate counts (HPC) and plating for faecal indicator bacteria as regular microbiological control parameters. The recent development of relative simple bench-top flow cytometers has made rapid and quantitative analysis of cultivation-independent microbial parameters more feasible than ever before. Here we present a study using a combination of cultivation-independent methods including fluorescence staining (for membrane integrity, membrane potential and esterase activity) combined with flow cytometry and total adenosine tri-phosphate (ATP) measurements, to assess microbial viability in drinking water. We have applied the methods to different drinking water samples including non-chlorinated household tap water, untreated natural spring water, and commercially available bottled water. We conclude that the esterase-positive cell fraction, the total ATP values and the high nucleic acid (HNA) bacterial fraction (from SYBR((R)) Green I staining) were most representative of the active/viable population in all of the water samples. These rapid methods present an alternative way to assess the general microbial quality of drinking water as well as specific events that can occur during treatment and distribution, with equal application possibilities in research and routine analysis.     

EPA policies to protect the health of consumers of drinking water in the United States

In the United States our objective is to protect drinking water at the source,during treatment and during distribution. In 1975 interim regulations for bacteria and turbidity, 10 inorganic chemicals, 6 organic chemicals end radionuclides were promulgated. In 1979 National Seccndary Regulations for substances affecting the aesthetic quality of water were promulgated. In 1979 trihalomethanes were added, The United States is engaged in comprehensive revisions of the National Primary Drinking Water Regulations.The areas of our most significant concern include detection and control of contamination of ground waters by organic chemicals resulting from improper waste disposal practices, a reassessment of microbiological regulations and toxicity of disinfectant by-products and a major effort to deal with corrosion-related contamination of drinking water during distribution. We are evaluating the issue of a granular activated carbon requirement for contaminated surface waters. A program to assure the quality of direct and indirect additives to drinking water has also been initiated. Part of this activity will include determination of the contaminants and by-products associated with the use of various water treatment chemicals and pipe materials.     

Fluorescence monitoring at a recycled water treatment plant and associated dual distribution system – Implications for cross-connection detection

Dual distribution systems are becoming increasingly common in greenfield housing developments in Australia for the redistribution of recycled water to households for non-potable use. Within such schemes there exists the potential for cross-connections between recycled and drinking water systems. Due to the high level of recycled water treatment, these events are unlikely to lead to outbreaks of illness in the community. Nonetheless, they do represent a breach of the recycled water risk management strategy and therefore an elevated level of risk to consumers. Furthermore, cross-connection events have the potential to undermine public confidence in these types of water recycling. A rapid, highly sensitive method of cross-connection detection may therefore provide an additional level of confidence in these schemes. The aim of this research was to determine the potential for using fluorescence spectroscopy as a monitoring tool in water treatment plants and dual distribution systems. Samples from both the water recycling plant and dual distribution system were collected on a weekly basis over 12 weeks. Fluorescence excitation–emission matrix (EEM) spectra and water quality parameters including dissolved organic carbon, UV₂₅₄, pH, conductivity, free chlorine and turbidity were obtained for each sample. The fluorescence EEM spectra of recycled and drinking water were distinctly different and exhibited low variability throughout the course of the sampling program, indicating a degree of stability of the fluorescent components within the organic matter. A ten-fold difference in mean fluorescence intensity was observed for recycled water compared to drinking water, which was greater than the difference observed for the other measured water quality parameters. Probabilistic analysis was used to determine the reliable detection limit of recycled water contamination of drinking water. Accounting for the inherent variability of both recycled water and drinking water, a 45% contamination of recycled water in drinking water could be detected with a signal-to-noise ratio greater than 3 for more than 95% of individual random sample pairs. Greater sensitivity can be assured by averaging numerous samples. In comparison, a 70% contamination of recycled water in drinking water was required for the same detection using conductivity.     

Short- and long-term variations of norovirus concentrations in the Meuse river during a 2-year study period

Faecally impacted surface waters used for drinking water production may encompass risk for norovirus infections. To be able to assess a possible health risk, noroviruses should be quantified and fluctuations identified. In 2001, norovirus concentrations in the river Meuse displayed a seasonal distribution with high peaks during wintertime as determined by RT-PCR on serially diluted RNA. An intensified day-by-day sampling scheme in the winter of 2002/2003 revealed that the winter peak consisted of several peaks of varying duration and magnitude, possibly due to contamination events in the catchment. The highest estimated concentration was 1700 PCR-detectable units per litre (95% CI 250-8000), which if coinciding with failing treatment could lead to significant numbers in drinking water. Adaptive dynamic filtering was shown to adequately predict subsequent sample concentrations. If valid, such analyses could prove to be useful as early warning systems in risk management of water sources.     

Microbiological quality of water from hand-dug wells used for domestic purposes in urban communities in Kumasi,Ghana

Assessment was done on the microbiological quality of water in hand-dug wells in urban communities in Kumasi, Ghana. A total of 256 water samples were taken from eight wells and examined for faecal coliforms, enterococci and helminths. High contamination levels were recorded in the wells, more so in the wet season, with faecal coliforms levels between 6.44 and 10.19 log units and faecal enterococci between 4.23 and 4.85 CFU per 100 ml. Influence on protection and lining of wells on water quality was not pronounced but mechanization reduced contamination significantly by about 3 log units. This study shows a stronger influence of poor sanitation and improper placement of wells on water quality compared to improvements made from lining and protection of wells. In the race to increase access to drinking water in poor urban settlements, quality of groundwater could be a major barrier, if provision of drinking water is not matched with improvements in sanitation and urban planning.     

Assessing the public health risk of microbial intrusion events in distribution systems: conceptual model, available data, and challenges

Low and negative pressure events in drinking water distribution systems have the potential to result in intrusion of pathogenic microorganisms if an external source of contamination is present (e.g., nearby leaking sewer main) and there is a pathway for contaminant entry (e.g., leaks in drinking water main). While the public health risk associated with such events is not well understood, quantitative microbial risk assessment can be used to estimate such risk. A conceptual model is provided and the state of knowledge, current assumptions, and challenges associated with the conceptual model parameters are presented. This review provides a characterization of the causes, magnitudes, durations and frequencies of low/negative pressure events; pathways for pathogen entry; pathogen occurrence in external sources of contamination; volumes of water that may enter through the different pathways; fate and transport of pathogens from the pathways of entry to customer taps; pathogen exposure to populations consuming the drinking water; and risk associated with pathogen exposure.     

The concept of choosing a list of indices and their regulatory values for determination of hygienic requirements and control over the drinking water quality in Ukraine

The paper presents new conceptual approaches to establishing indices for the quality of physiologically wholesome drinking water for the population based on the analysis of the composition of surface and underground sources of water supply in Ukraine. We have proposed ecological regulations for the quality of Ukraine’s surface waters as a basis for the choice of quality values of these indices. These regulations provide effectively for the absence of anthropogenic contamination of natural sources of water supply. We have also considered present-day approaches to regulating maximum admissible concentrations (MAC) of drinking water impurities during its conditioning at the stations of centralized water supply. We have provided qualitative MAC values of water impurities, which may be recommended taking into account technological and economic possibilities.     

浅析潍坊市中心市区城市供水水源地水质情况

针对潍坊市两处主要水源地水质情况进行了连续3年19项指标的监测分析研究,并对其中的感官性状和一般化学指标、有机综合指标、毒理指标、生物指标等关键指标进行了重点分析,根据年均值、最大值、最小值的变化情况,对潍坊市水源水质污染的发展趋势做出了合理总结和评估.结果表明,一些重要的毒理指标正呈现逐渐下滑的趋势,波动幅度也逐渐减小;但从藻类指标来看,两处水源均为高藻水源且近年来显示为不同程度的上升.该结果可应用于潍坊市中心城区饮用水水源水质的风险预控,有助于潍坊市中心城区水源水质的风险管理.     

Fecal bacteria in the rivers of the Seine drainage network (France): sources, fate and modelling

The Seine river watershed (France) is a deeply anthropogenically impacted area, due to the high population density, intense industrial activities and intensive agriculture. The water quality and ecological functioning of the different rivers of the Seine drainage network have been extensively studied during the last fifteen years within the framework of a large French multidisciplinary scientific program (PIREN Seine program). This paper presents a synthesis of the main data gained in the scope of this program concerning the microbiological water contamination of the rivers of the Seine drainage network. The more common indicator of fecal contamination (fecal coliforms) was mainly used; some complementary works used E. coli and intestinal enterococci as alternative fecal indicators. Point sources (outfall of wastewater treatment plants) and non point sources (surface runoff and soil leaching) of fecal pollution to the rivers of the watershed were quantified. Results showed that, at the scale of a large urbanised watershed as the Seine basin, the input of fecal micro-organisms by non-point sources is much lower than the inputs by point sources. However, the local impact of diffuse non-human sources (especially surface runoff of pastured fields) can be of major importance on the microbiological quality of small headwater rivers. Fecal contamination of the main rivers of the Seine watershed (Seine, Marne, Oise rivers) was studied showing high level of microbiological pollution when compared to European guidelines for bathing waters. The strong negative impact of treated wastewater effluents outfall on the microbiological quality of receiving rivers was observed in different areas of the watershed. Once released in rivers, culturable fecal bacteria disappeared relatively rapidly due to mortality (protozoan grazing, lysis) or loss of culturability induced by stress conditions (sunlight effect, nutrient concentration, temperature). Mortality rates of E. coli were studied in different types of rivers within the watershed showing, in summer conditions, no major difference in the mortality rates in small and large rivers. As a result of these studies, a module describing the dynamics of fecal bacteria has been developed and embedded within a hydro-ecological model describing the functioning of the rivers of the whole watershed (the SENEQUE model). Once validated, such a model can be used for testing predictive scenarios and thus can be a very useful tool for the management of microbiological water quality at the scale of the whole basin.     

The quest for safe drinking water: an example from Guinea-Bissau (West Africa)

While humans require water for life, one-sixth of our species lives without access to safe water. In Africa, the situation is particularly acute because of global warming, the progression of the Sahara desert, civil unrest and poor governance, population growth, migration and poverty. In rural areas, the lack of adequate safe water and sanitary infrastructures leaves millions with doubtful water quality, increasing the harshness of daily life. In this paper, a pilot study was conducted during the wet season on Bolama Island (Guinea-Bissau, West Africa), a UNESCO Man and the Biosphere Reserve. Twenty-eight shallow wells, supplying water to most of the population, were sampled for microbiological, physical and chemical water quality characteristics. A ten-parameter water quality index (WQI) adapted to tropical conditions was applied to compare the different wells. About 79% of the wells showed moderate to heavy fecal contamination. From the surveyed parameters, it was found that chemical contamination was less important, although all samples were acidic, with the pH averaging 5.12+/-0.08. The WQI was 43+/-4% (0%-worst; 100%-best quality), showing that the water from the majority of wells was polluted but should be suitable for domestic use after appropriate treatment. At the onset of the wet season, diarrhea represented 11.5% of all medical cases, 92.5% of which were children aged <15. This paper suggests inexpensive steps to reduce the fecal contamination and control the pH in order to increase the potability of the well water and, concomitantly, to raise the living standards of the population in one of the poorest countries of the world.     

Survival of infectious Poliovirus-1 in river water compared to the persistence of somatic coliphages, thermotolerant coliforms and Poliovirus-1 genome

The microbiological quality of water is currently assessed by search for fecal bacteria indicators. There is, however, a body of knowledge demonstrating that bacterial indicators are less resistant to environmental factors than human pathogenic viruses and therefore underestimate the viral risk. As river water is often used as a resource for drinking water production, it is particularly important to obtain a valid estimation of the health hazard, including specific viral risk. This work was conducted to compare the survival of infectious Poliovirus-1 used as a pathogenic virus model to the persistence of, on the one hand, thermotolerant coliforms commonly used as indicators and on the other hand, to somatic coliphages and Poliovirus-1 genome considered as potential indicators. We studied the behavior of infectious Poliovirus-1 and the three (potential) indicators of viral contamination in river water at three different temperatures (4 degrees C,18 degrees C and 25 degrees C). This experiment was performed twice with river water sampled at two different periods, once in winter and once in summer. Our results showed that the survival of thermotolerant coliforms can be 1.5-fold lower than infectious Poliovirus-1. In contrast, under all our experimental conditions, somatic coliphages and Poliovirus-1 genome persisted longer than infectious Poliovirus-1, surviving, respectively, 2-6-fold and about 2-fold longer than infectious Poliovirus-1. According to our results exclusively based on survival capacity, somatic coliphages and viral genome, unlike thermotolerant coliforms appear to be better indicators of viral contamination in river water. Moreover, the disappearance of viral genome is well-correlated to that one of infectious virus irrespective of the conditions tested.     

Survey of users and providers of recycled water: quality concerns and directions for applied research

Users and providers of recycled water in Queensland, Australia, were canvassed to ascertain concerns about recycled water quality and directions for applied research. Some 79% of respondents had concerns about recycled water quality including microbiological components, such as viruses, parasites and bacteria, salinity related components, aggregate components, such as pH and solids, nutrients, organic components, including pesticides and endocrine disruptors, as well as quality variability. Relative differences in the proportion of concern for some quality characteristics were observed between the areas of different population density with those from the low population areas being more concerned than those from medium or high areas. Some users and providers observed changes in recycled water quality during distribution including nutrients, chlorine, solids, colour and odour. Some 52% of providers and 19% of current users propose to expand their usage and 30% of non-users propose to commence doing so in the next 5 years. Recycled water quality characteristics identified for applied research included microbiological components, organic components, nutrients and salinity.     

On-site infiltration of a copper roof runoff: role of clinoptilolite as an artificial barrier material

On-site infiltration may be considered as a promising way of managing rainwater runoffs in urban areas, provided the hydrological and ecological conditions allow infiltration, and provided there is adequate treatment of the contaminants to avoid a risk of soil and groundwater pollution. The aim of this study was to evaluate the feasibility of the application of a new technical infiltration system equipped with clinoptilolite as an artificial barrier material for the treatment of the copper roof runoff of the Academy of Fine Arts in Munich, Germany. During the 2-yr sampling period, 30 rain events were examined. The cover material of the roof and the drainage system was responsible for the high copper concentrations in the roof runoff. The rain height and the rain intensity were of great significance regarding the establishment of the copper runoff rate. The technical infiltration system applied was able to reduce the copper from the roof runoff by a factor up to 96%. The mean measured copper concentration in percolation water was lower than the critical value of 50 microg/l set by the German Federal Soil Protection Act and Ordinance, indicating no risk for soil and groundwater contamination.     

Contribution of treated wastewater to the microbiological quality of Seine River in Paris

Urban part of Seine River serving as drinking water supply in Paris can be heavily contaminated by Cryptosporidium spp. and Giardia duodenalis. In the absence of agricultural practice in this highly urbanized area, we investigated herein the contribution of treated wastewater to the microbiological quality of this river focusing on these two parasites. Other microorganisms such as faecal bacterial indicators, enteroviruses and oocysts of Toxoplasma gondii were assessed concurrently. Raw wastewaters were heavily contaminated by Cryptosporidium and Giardia (oo)cysts, whereas concentrations of both protozoa in treated wastewater were lower. Treated wastewater, flowed into Seine River, had a parasite concentration closed to the one found along the river, in particular at the entry of a drinking water plant (DWP). Even if faecal bacteria were reliable indicators of a reduction in parasite concentrations during the wastewater treatment, they were not correlated to protozoal contamination of wastewater and river water. Oocysts of T. gondii were not found in both raw and treated wastewater, or in Seine River. Parasitic contamination was shown to be constant in the Seine River up to 40 km upstream Paris. Altogether, these results strongly suggest that treated wastewater does not contribute to the main parasitic contamination of the Seine River usually observed in this urbanized area.     

Monitoring of water quality from roof runoff: Interpretation using multivariate analysis

The quality of harvested rainwater used for toilet flushing in a private house in the south-west of France was assessed over a one-year period. Temperature, pH, conductivity, colour, turbidity, anions, cations, alkalinity, total hardness and total organic carbon were screened using standard analytical techniques. Total flora at 22 °C and 36 °C, total coliforms, Escherichia coli and enterococci were analysed. Overall, the collected rainwater had good physicochemical quality but did not meet the requirements for drinking water. The stored rainwater is characterised by low conductivity, hardness and alkalinity compared to mains water. Three widely used bacterial indicators - total coliforms, E. coli and enterococci - were detected in the majority of samples, indicating microbiological contamination of the water. To elucidate factors affecting the rainwater composition, principal component analysis and cluster analysis were applied to the complete data set of 50 observations. Chemical and microbiological parameters fluctuated during the course of the study, with the highest levels of microbiological contamination observed in roof runoffs collected during the summer. E. coli and enterococci occurred simultaneously, and their presence was linked to precipitation. Runoff quality is also unpredictable because it is sensitive to the weather. Cluster analysis differentiated three clusters: ionic composition, parameters linked with the microbiological load and indicators of faecal contamination. In future surveys, parameters from these three groups will be simultaneously monitored to more accurately characterise roof-collected rainwater.     

Predicting runoff-induced pesticide input in agricultural sub-catchment surface waters: linking catchment variables and contamination

An urgent need exists for applicable methods to predict areas at risk of pesticide contamination within agricultural catchments. As such, an attempt was made to predict and validate contamination in nine separate sub-catchments of the Lourens River, South Africa, through use of a geographic information system (GIS)-based runoff model, which incorporates geographical catchment variables and physicochemical characteristics of applied pesticides. We compared the results of the prediction with measured contamination in water and suspended sediment samples collected during runoff conditions in tributaries discharging these sub-catchments. The most common insecticides applied and detected in the catchment over a 3-year sampling period were azinphos-methyl (AZP), chlorpyrifos (CPF) and endosulfan (END). AZP was predominantly found in water samples, while CPF and END were detected at higher levels in the suspended particle samples. We found positive (p<0.002) correlations between the predicted average loss and the concentrations of the three insecticides both in water and suspended sediments (r between 0.87 and 0.94). Two sites in the sub-catchment were identified as posing the greatest risk to the Lourens River mainstream. It is assumed that lack of buffer strips, presence of erosion rills and high slopes are the main variables responsible for the high contamination at these sites. We conclude that this approach to predict runoff-related surface water contamination may serve as a powerful tool for risk assessment and management in South African orchard areas.     

Flow cytometry and adenosine tri-phosphate analysis: alternative possibilities to evaluate major bacteriological changes in drinking water treatment and distribution systems

An ever-growing need exists for rapid, quantitative and meaningful methods to quantify and characterize the effect of different treatment steps on the microbiological processes and events that occur during drinking water treatment and distribution. Here we compared cultivation-independent flow cytometry (FCM) and adenosine tri-phosphate (ATP) analysis with conventional cultivation-based microbiological methods, on water samples from two full-scale treatment and distribution systems. The two systems consist of nearly identical treatment trains, but their raw water quality and pre-treatment differed significantly. All of the drinking water treatment processes affected the microbiological content of the water considerably, but once treated, the finished water remained remarkably stable throughout the distribution system. Both the FCM and ATP data were able to describe the microbiology of the systems accurately, providing meaningful process data when combined with other parameters such as dissolved organic carbon analysis. Importantly, the results highlighted a complimentary value of the two independent methods: while similar trends were mostly observed, variations in ATP-per-cell values between water samples were adequately explained by differences in the FCM fingerprints of the samples. This work demonstrates the value of alternative microbial methods for process/system control, optimization and routine monitoring of the general microbial quality of water during treatment and distribution.