Storage and display characteristics of electrically stimulated, hot-boned and nonstimulated, cold-boned beef

One side, randomly selected from each of 33 carcasses from forage-fed steers, was electrically stimulated within 30 min of exsanguination and hot-bonded (ESHB). Opposite sides were not electrically stimulated and were cold-boned (NESCB) following a 24 h chill (0°C). From 17 ESHB sides, portions of the longissimus dorsi (LD) and semimembranosus (SM) muscles were removed and immediately vacuum packaged. LD and SM muscles were removed from the remaining 16 ESHB sides, wrapped in polyvinyl chloride film, chilled (0°C) for 22 h and then vacuum packaged. LD and SM muscles from NESCB sides (n = 33) were removed and the vacuum packed at 24 h post mortem. Whole muscle characteristics were evaluated initially and after storage periods of 7, 14 or 21 days and steaks were displayesd for 5 years thereafter. Neither hot versus cold-boning nor storage for 21 days affected retail display characteristics of LD and SM steaks (P > 0·05). As retail display time increased, retail lean color, fat color and overall appearance scores decreased.     

Effects of Electrical Stimulation, Hot Boning and Chilling on Bull Semimembranosus Muscle

Taste panel, Warner-Bratzler shear force (WBS) and cooking loss characteristics of semimembranosus (SM) muscles from thirty electrically stimulated and hot-boned (ESHB) or control young bull sides were evaluated. At 2 hr postmortem, muscles were excised from electrically stimulated sides and chilled in a tray or in a cardboard box. All control sides and ESHB muscles were chilled at 5–7°C until 24 hr postmortem; then 2–4°C for 24 hr. At 48 hr postmortem, steaks were cut, vacuum packaged and aged (2–4°C) for 4 days. Beef flavor was more intense for control steaks than for steaks from either ESHB chilling treatment. No other differences were noted.     

Effects of Electrical Stimulation, Aging, and Blade Tenderization on Hot-Boned Beef Psoas major and Triceps brachii Muscles

Forty-six steer carcasses were used to evaluate shear force values (SFV) for triceps brachii (TB) and psoas major (PM) muscles from sides assigned to three treatments: (1) chilled at 2–4°C for 48 hr (C); (2) hot boned 2 hr postmortem (HB); and (3) electrically stimulated 1 hr postmortem and hot boned 2 hr postmortem (ESHB). Some steaks were cut and frozen immediately after muscle excision or after 6 days of aging, and some were blade tenderized. HB and ESHB steaks had equal or smaller SFV relative to C after aging the TB and PM muscles; however, this was not true when TB steaks were cut after muscle excision. Electrical stimulation or blade tenderization did not improve HB.     

EARLY POSTMORTEM SKELETAL ALTERATIONS EFFECT ON SARCOMERE LENGTH, MYOFIBRILLAR FRAGMENTATION, AND MUSCLE TENDERNESS OF BEEF FROM LIGHT-WEIGHT, BRANGUS HEIFERS

Approximately 2 h after exsanguination, two skeletal cuts (TC) were made on randomly selected sides from 13 light-weight, Brangus heifers; companion sides served as untreated controls (C). After a 24-h chill period, four muscles (Longissimus muscle from the IMPS#112A ribeye roll, LT, and from the IMPS#180 strip loin, LL; Biceps femoris, BF; Semitendinosus, ST; and Semimembranosus, SM) were excised from each side, vacuum-packaged, and aged for 7 days. Five 2.54-cm thick steaks were cut from each of the muscles to determine the effectiveness of the TC treatment on sarcomere length (SL), myofibrillar fragmentation index (MFI) and cooked beef tenderness. The LT and LL steaks from TC-carcasses had longer (P < 0.05) sarcomeres, and the TC treatment decreased (P < 0.05) Warner-Bratzler shear (WBS) force values for the LT and LL by 35.8 and 33.2%, respectively. Moreover, SL of the muscles from the round were also increased (P < 0.05) by the TC procedure, but WBS force values were not different (P > 0.05) from C. Muscles from the TC sides had similar (P > 0.05) MFI values to those from control sides. This study demonstrated that the TC procedure, when applied 2 h postmortem, can effectively improve cooked beef tenderness of the Longissimus muscle .     

Effects of Certain Electrical Stimulation Parameters on Quality and Palatability of Beef

Grain-finished steers and heifers (n=390) were slaughtered in a commercial plant and assigned to one of five groups: (a) electrical stimulation (ES) immediately after exsanguination, (b) ES immediately before evisceration, (c) ES immediately after evisceration, (d) ES immediately after splitting, and (e) untreated (not ES). ES consisted of either 150 or 550 volts for either 1 or 2 mm. Electrically stimulated carcasses had more youthful, brighter colored lean and less “heat-ring” than untreated (not ES) carcasses. Steaks from electrically stimulated carcasses had (P<0.05) lower shear force values, were more tender, had less panel-detectable connective tissue and higher overall palatability ratings than did steaks from untreated (not ES) carcasses. Steaks from carcasses receiving 550-volt ES usually had more youthful, brighter colored lean and lower shear force values, were more tender, had less panel-detectable connective tissue and higher overall palatability ratings than steaks from carcasses that received 150-volt electrical stimulation. Quality and palatability traits of carcasses electrically stimulated for 1 mm were not different from those of carcasses receiving 2 mm of electrical stimulation. Electrical stimulation at different stages in the slaughter-dressing sequence did not evoke differential responses in quality or palatability factors.     

Singular and combined effects of electrical stimulation, post-mortem ageing and blade tenderisation on the palatability attributes of beef from young bulls

Thirty Santa Gertrudis bulls (approximately 15-18 months old) were slaughtered, dressed and split into siides. The right side of each carcass was electrically stimulated (ES) with seventeen impulses (1·8s impulse duration; 1·8s interval between impulses) of 550 V (AC) and 5 A while the left side served as a non-stimulated control (not-ES). At 24h post morten, USDA quality and yield grade data were obtained from each side. On the second day post mortem, all sides were fabricated and strip loins, top sirloin butts and ribeyes were obtained from each side for post-mortem ageing and blade tenderisation studies. Steaks were removed after a post-mortem ageing period of 4 or 18 days and before (not-BT) or after blade tenderisation (BT) for sensory panel evaluations or shear force determinations. ES sides had more youthful lean maturity (P < 0·0001), higher marbling (P < 0.·002), higher USDA quality grades (P < 0·0.0001) and finer-textured lean (P < 0·002) than did not-stimulated (not-ES) sides. ES significantly improved (P < 0·05) palatability traits in two of twenty-four comparisons; BT significantly improved palatability traits in twelve of twenty-four comparisons and 18-day post-mortem ageing significantly improved palatability traits in seven of twelve comparisons. No significant reductions (P < 0·05) in shear force values were observed for steaks from ES versus not-ES sides while significant reductions (P < 0·05) were observed for steaks from BT versus non-BT cuts (four of six comparisons) and for steaks from cuts aged for 18 versus 4 days (ten of twelve comparisons). BT and 18-day post-mortem ageing were more effective for increasing palatability or for decreasing shear force requirements than was ES; however, ES greatly improved lean colour of meat from bulls.     

Effects of Beef Carcass Electrical Stimulation, Hot Boning, and Aging on Unfrozen and Frozen Longissimus dorsi and Semimembranosus Steaks

Ninety-six sides from 48 beef carcasses were used to study the effects of hot boning (HB), electrical stimulation (ES), a combination of electrical stimulation and hot boning (ESHB), and steak storage treatments on longissimus dorsi and semimembranosus steaks. Steak storage treatments were: unfrozen (aged until 6 days postmortem) or frozen (ES, HB, and ESHB frozen 24 hr and controls 48 hr postmortem). ES did not improve taste panel ratings or consistently lower shear force values when compared with control counterparts. Storing carcasses at 5°C for the first 24 hr postmortem and freezing ES steaks at 24 hr versus 48 hr postmortem for the control possibly diluted the effectiveness of ES. However, ES did eliminate any toughening due to HB.     

EFFECTS OF SOLUTION ENHANCEMENT ON PALATABILITY AND SHELF-LIFE CHARACTERISTICS OF LAMB RETAIL CUTS

The low demand for lamb in the U.S.A. requires the sheep industry to focus on the development of a means to simultaneously protect fresh lamb consumption from deteriorating and entice new consumer purchases based on palatability characteristics. Thus, the shelf-life and palatability attributes of lamb enhanced with salt, phosphate and rosemary were compared with traditional, fresh, nonenhanced lamb cuts. Wholesale loins and legs (n  =  30/subprimal) were either injected to 112.5% of raw weight with the enhancement treatment (ENH) or not (Ctrl), and both ENH and Ctrl cuts were vacuum-packaged and stored 24 h before fabrication into 2.54-cm-thick chops/steaks. While color scores decreased (P  <  0.05) for both loin chops and leg steaks over the 7-day display period, sensory panelists preferred (P  <  0.05) ENH lamb over Ctrl chops and steaks. Results indicate that enhancement can improve the palatability attributes of lamb without detrimental effects on product shelf life.

PRACTICAL APPLICATIONS

Information presented in the current experiment can be used as a tool to define the sensory taste and color quality characteristics of solution-enhanced lamb retail cuts. More specifically, this information could be used to support future studies in the areas of color stability/packaging applications and a means to identify a threshold for solution enhancement of lamb muscles. With an industry that relies on the identification of new cuts for the retail market, and consumers placing tremendous emphasis on muscle color at the time of purchase, a thorough understanding of color stability for solution-enhanced lamb is necessary. Lastly, this information can be used to further enhance the potential marketability of such muscles from the lamb carcass that tend to receive severe discounts as a result of a lack in consumer preference for lamb products in the retail setting.     

Palatability characteristics of loin steaks from Charolais crossbred bulls and steers

Loins from 1/2-blood or 3/4- to 7/8-blood Charolais bulls (n = 60) and steers (n = 64) were obtained at 24 h post mortem from electrically stimulated sides and, at 12 days post mortem, steaks were removed and frozen. Shear force values were higher (P < 0·001) and sensory panel evaluations of tenderness and overall palatability were lower (P < 0·001) for steaks from bulls than from steers. No differences (P > 0·05) in connective tissue amount or off-flavour were detected for steaks from bulls versus steers. Significant correlations between carcass secondary sex characteristics and sensory panel evaluations of tenderness were noted, but the coefficients were not of a magnitude to be considered predictive of tenderness.     

Effects of Beef Carcass Electrical Stimulation and Hot Boning on Display Color of Unfrozen Vacuum Packaged Steaks

Ninety-six beef sides from 48 carcasses were used to determine effects of control (C, chilled 48 hr at 5°C), electrical stimulation (ES, 45 min postmortem, 400 volts for 2 min, pulsed), and hot boning (HB, 2 hr postmortem), and combination (ESHB) treatments on muscle color of longissimus (LD) and semimembranosus (SM), vacuum packaged steaks. HB muscles frequently were visually brighter purplish-red than other treatments. Compared to ESHB, ES LD was not different, but ES SM was duller purplish-red in color. Reflectance indicators of reduced myoglobin and metmyoglobin were essentially the same across treatments in both muscles. Vacuum packaged fresh beef steaks from all treatments were acceptable in color at 0, 3, 7, and 14 days of display. Vacuum packaging appears suitable for steaks from any of these carcass treatments but is especially useful for steaks from hot boned cuts.     

ACCELERATED RESTRUCTURING OF BEEF

Restructured steaks manufactured by two comminution methods from prerigor electrically stimulated (PES) beef, nonstimulated counterparts (NES) and postrigor non-electrically stimulated (C) samples were compared. After frozen storage for 0,14, and 42 days, samples were subjectively evaluated for appearance and palatability traits. Objective measurements included Hunter Color, TBA, Kramer, and Instron values. Electrical stimulation reduced peak force but did not consistently improve sensory panel scores. Comminution method did not consistently affect any traits. Results suggested that subjective appearance was affected more by storage time than other treatments and that prerigor beef may be effectively utilized in restructured beef without affecting palatability traits.     

Palatability of bison semimembranosus and effects of marination

The present study was conducted to investigate the palatability of bison semimembranosus muscle (SM) and the effects of injection with sodium chloride and sodium tripolyphosphate on cooking yield, colour, shear force and consumer acceptability. Twenty paired SM were obtained from 10 intact male bison (aged 24-30 months); each of these muscles was divided longitudinally into two sections. One section was injected to 110% of its original weight to contain 0.5% sodium chloride and 0.3% sodium tripolyphosphate, while the other was kept as a non-injected control. HunterLab a* (redness) and b* (yellowness) values did not differ (P>0.05) between injection treatments; however, injected steaks had lower L* values (darker) compared to controls. Control samples from bison SM were very lean and high in protein but not very tender. Marination by injection was able to significantly reduce shear force values of SM; that is, injected steaks/roasts had significantly (P<0.001) lower shear force values (63.9 N) compared to control samples (102.3 N). Cooking yields for the steaks/roasts from the injected sections were significantly (P<0.001) higher compared to those from control non-injected sections when cooked to either 71 or 77?°C. Bison samples cooked by moist-heat had significantly (P<0.001) lower cooking losses and shear force values compared to those cooked by dry-heat. As expected, steaks/roasts were more tender and had higher cooking yields when cooked to a medium level of doneness (71?°C) compared to an internal temperature of 77?°C (well done). A panel of 80 consumers preferred injected steaks cooked to 77?°C endpoint over other combinations, followed by non-injected steaks cooked to 71?°C, whereas injected steaks cooked to 71?°C and non-injected steaks cooked to 77?°C were equally but least preferred. Hence, injection seems to be protecting against moisture loss at high end-point cooking temperatures.     

Effects of chilling methods and hot-boning on quality parameters of M. longissimus lumborum from Bos indicus Nelore steer

To study the effects of chilling methods and hot-boning on quality parameters of M. longissimus lumborum (LL) of Nelore steers, sixteen left-carcass sides were electrically stimulated and the LL muscles were hot-boned and chilled at − 20 °C (HBVFC) or 0 °C (HBO). Eight control left-carcass sides were cold-boned (AT). All muscles were vacuum-packaged and aged at 0 °C for 14 d. Shear force and tenderness of the AT-treated muscles were not different from HBO-treated muscles. The shear force values of the HBVFC muscles were higher after 7 and 14 d post-mortem (pm) compared to those of the AT muscles, but there was no difference from the HBO muscles. Aging did not reduce the shear force values of the HBVFC muscles. The purge losses of the HBVFC muscles were higher than those of the HBO and AT muscles. The HBVFC muscles were less tender than the HBO and AT muscles at 14 d pm.     

RELATIONSHIP OF USDA MARBLING GROUPS TO PALATABILITY OF COOKED BEEF

As marbling increased from“practically devoid” to“moderately abundant”, loin steaks were more palatable (P<0.05) about 2/3 of the time, round steaks were more palatable (P<0.05) about 1/8 of the time, and loin steaks were more likely to be assigned high (≥6.00) panel ratings and to have low (≤3.63 kg) shear values. However, increases in marbling from“slight” to“moderately abundant” (A maturity) and from“small” to“moderately abundant” (A+B maturity) had little or no efect on percentage incidence of loin or round steaks with panel ratings ≤2.99 or ≥4.00, or with shear values ≥6.35 kg or ≤4.99 kg. Differences in marbling explained about 33% (loin) and 7% (top round) of the variation in overall palatability ratings in A, B, C, and A+B maturity carcasses.     

EFFECT OF INDIVIDUAL MUSCLES AND CONNECTIVE TISSUE LEVELS ON THE SENSORY AND SHEAR PROPERTIES OF RESTRUCTURED BEEF STEAKS

Restructured steaks were made from slices of forequarter muscles known to be different in tenderness. Additional steaks were made from pieces that were sorted to contain either epimysium, intramuscular connective tissue sheets or no visible connective tissue. Texture panels and shear force values could discriminate between steaks from some of the different muscles. High correlations ( P < 0.05) existed between connective tissue amounts and the sensory evaluations of residual connective tissue toughness and instrumental shear force values.     

Effects of sodium chloride, phosphate type and concentration, and pump rate on beef biceps femoris quality and sensory characteristics

Beef biceps femoris muscles (n=45) were used to evaluate the effect of enhancement with solutions comprising 2.0% sodium chloride and either sodium hexametaphosphate (SHMP), sodium tripolyphosphate (STPP), or tetrasodium pyrophosphate (TSPP) at either 0.2% or 0.4% of product weight. All solutions were injected into muscle samples at either 112% (12% pump) or 118% (18% pump) of raw product weight. Muscles treated with all three phosphate types had decreased (P<0.05) free water compared to untreated muscles (CNT), and while TSPP-treated muscles were able to bind greater (P<0.05) additional water than CNT, STPP- and SHMP-treated muscles did not differ (P>0.05) from CNT. Disregarding phosphate type, steaks with 0.4% phosphate inclusion bound more (P<0.05) water than those with 0.2% phosphate inclusion. Steaks treated with STPP or TSPP had decreased (P<0.05) cooking losses than CNT, while SHMP-treated steaks did not differ (P>0.05) from CNT. Steaks injected at 18% pump had greater (P<0.05) percent moisture, and did not differ (P>0.05) in free water, water binding, or cooking losses from steaks injected at 12% pump. Although there were no differences (P>0.05) in Warner-Bratzler shear force in this study, steaks with SHMP, STPP, and TSPP all were rated more tender, and juicier (P<0.05) by sensory panelists than CNT steaks or steaks enhanced only with sodium chloride. Regardless of phosphate type, steaks enhanced with 0.4% phosphate and those steaks at 18% pump received improved (P<0.05) sensory tenderness ratings compared to 0.2% phosphate and 12% pump, respectively. These results suggest that enhancing biceps femoris muscles with STPP or TSPP can improve water retention, yield, and palatability characteristics. Additionally, enhancement with a phosphate/salt solution at an 18% pump rate, compared to a 12% pump rate, can allow for improved sensory tenderness perceptions without decreasing product yields.     

Quality, palatability and weight loss of pork as affected by electrical stimulation

Forty-five swine (n = 15, stress-susceptible pigs, fasted for 18 h; n = 15, normal pigs, fasted for 48, 60 or 72h; n = 15, normal pigs, fasted for 18 h) were slaughtered. One side of each pig was electrically stimulated (ES)-550 V, 2-6 A, seventeen impulses, 1·8 s on and 1·8 s off per impulse-and the opposite side was not stimulated (NS). Ham and loin temperatures were monitored at 12 and 24 hours post mortem. Carcass quality-marbling, loin eye colour, loin eye firmness, subcutaneous fat firmness and belly firmness-was evaluated at 24 hours post mortem. Sides were fabricated into four lean cuts at 48 hours; cuts were placed on open metal racks in a 2°C cooler and weight loss was measured after 24, 48 and 72 hours storage. At 120 hours post mortem, loin chops were cut, frozen (-24°C for 45 days) and subsequently used to determine thaw loss, cooking loss, Warner-Bratzler shear (WBS) force and palatability. Ham temperatures (12 h) were significantly (P < 0·05) lower for the ES sides from the stress-susceptible, short-fast (SSF) group but not for normal, long-fast (NLF) or normal, short-fast (NSF) groups. ES produced lighter coloured, softer loin eyes in the SSF group but darker coloured, firmer loin eyes and firmer subcutaneous fat in the NLF group. No significant differences (P > 0·05) were detected for thaw loss, cooking loss, palatability ratings or WBS values between ES and NS in the SSF group but chops from ES sides of NLF animals had significantly (P < 0·05) higher ratings for muscle fibre tenderness, overall tenderness and overall palatability. Weight losses of the lean cuts from the ES sides were higher (P < 0·05) than those from NS sides in only three of sixty comparisons of cuts from the three groups of swine. These data suggest that ES had very minimal effects on quality, palatability or weight loss of pork.     

Rapid chilling has no detrimental effect on the tenderness of low-voltage electrically stimulated M. longissimus in Chinese bulls

This study investigated the effect of pre-rigor rapid chilling (RC) on the rate of pH and temperature decline, shear force values and ultrastructure of M. longissimus from beef carcasses exposed to electrical stimulation (ES). Chinese bull carcasses were electrically stimulated, and the alternate sides of the carcasses either were chilled conventionally (CC, 0-4°C, air speed 0.5 m/s for 24 h) or they underwent RC (-14±1°C, air speed 3 m/s for 2 h and then cooled under CC conditions until 24 h post-mortem). The results showed that RC increased the rate of temperature decline (P<0.05) and decreased the rate of pH decline (P<0.05). There were no significant differences in the shear force value and sarcomere length of M. longissimus between the two treatments (P>0.05). The results of this study indicate that RC has no detrimental impact on the tenderness of beef carcasses exposed to ES.     

Solution Enhancement and Post-enhancement Storage Effects on the Quality, Sensory, and Retail Display Characteristics of Beef Triceps Brachii Muscles

Beef triceps brachii muscles (6 d postmortem; n= 15; muscle sections n= 45) were sectioned into 3rds and allocated to 1 of 3 treatments. The treatments were untreated (CNT), or injected at a 12% pump rate with either tap water‐only (H₂O) or a solution comprising tetrasodium pyrophosphate and sodium chloride (TSPP/ NaCl) at 0.4% and 1.0% target final product weight concentrations, respectively. Each muscle (comprising all 3 treatments) was then allocated to 2, 14, or 28 d of vacuum‐packaged 1°C storage. Purge losses during storage were greatest (P < 0.05) for H₂O muscles and least (P < 0.05) for TSPP/NaCl muscles. Purge losses also increased (P < 0.05) from 2 d to 14 d of storage. Steaks enhanced with TSPP/NaCl had less (P < 0.05) free water and lower (P < 0.05) cooking losses than either CNT or H₂O steaks. Storage duration did not affect (P > 0.05) Warner‐Bratzler shear force (WBS) or sensory tenderness, but juiciness decreased (P < 0.05) with increased storage duration. While storage duration did not impact (P > 0.05) instrumental color characteristics, aerobic plate counts generally increased during storage. The TSPP/NaCl steaks had lower (P < 0.05) WBS values and improved (P < 0.05) sensory tenderness and juiciness characteristics compared with CNT or H₂O steaks. While CNT steaks had greater (P < 0.05) L* values (lightness) than TSPP/NaCl steaks, TSPP/NaCl steaks had similar (P > 0.05) oxymyoglobin proportions (630/580 nm) and a* values (redness) as CNT steaks. These results suggest enhancement with TSPP/NaCl can improve triceps brachii yield and palatability characteristics. Increased post‐enhancement storage did not impact or worsened palatability while increasing purge losses, suggesting general deleterious effects of increased postmortem storage for this muscle.     

Effects of cooking beef muscles from frozen or thawed states on cooking traits and palatability

Biceps femoris and longissimus lumborum steaks were cooked from either a frozen or thawed state on an electric belt grill (TBG-60 MagiKitch'n Inc., Quakertown, PA) at 93?°C to the endpoint temperature of 70?°C. Cooking loss, cooking time, Warner-Bratzler shear force (WBSF), and color (Illuminant A, L(?), a(?), b(?)) were evaluated. Trained panelists (n=6) evaluated palatability attributes on an eight-point scale for myofibrillar tenderness, juiciness, flavor, overall tenderness, and connective tissue amount (1=extremely tough, dry, bland, tough, and abundant; 8=extremely tender, juicy, intense, tender, and none). L(?), a(?), WBSF, juiciness, flavor, connective tissue amount, and overall tenderness did not differ (P>0.05) between steaks cooked from frozen and thawed states. However, thawed steaks cooked faster and had lower cooking losses than frozen steaks. The biceps femoris had higher WBSF values than the longissimus lumborum and was rated less tender by trained panelists. L(?), a(?), or b(?) values did not differ (P>0.05) between muscles. The biceps femoris needed more time to cook and had greater cooking losses than longissimus lumborum. Dimensional changes of steaks cooked to either 60 or 70?°C were studied. The width of biceps femoris steaks decreased 12.2%, and width of longissimus lumborum decreased 6.3%. Longissimus lumborum steaks became shorter (P<0.01) than biceps femoris steaks. Thickness of biceps femoris steaks decreased 25.6%, and thickness of longissimus lumborum steaks decreased 23.3%. Muscle type had a more pronounced effect on dimensional changes of steaks than endpoint temperature.