Deer diet affects ribotype diversity of Escherichia coli for bacterial source tracking

Ribotyping is one of a number of genotypic methods for bacterial source tracking. This method requires a host origin database of one bacterial species be established in order to identify environmental isolates. Researchers establishing these databases have observed considerable ribotype diversity within a specific bacterial species. One source of this diversity may be diet. We determined the effect of diet on ribotype diversity for Escherichia coli in penned and wild deer (Odocoileus virginianus) in a 13-ha forested watershed. A total of 298 E. coli isolates was obtained, 100 from penned deer, 100 from wild deer, and 98 from the stream in the watershed to which all deer had access. The wild deer had significantly more ribotypes (35) than the penned deer (11 ribotypes, p = 0.05). This result suggests that diet affected ribotype diversity, and that a host origin database for bacterial source tracking should contain bacterial isolates from wild rather than from captive animals. Also, 42 of 98 (42.9%) environmental isolates matched penned and wild deer ribotypes. If bacterial source tracking determines that fecal contamination is predominantly from wildlife, then it may be unnecessary to monitor these watersheds because control over wildlife is difficult.     

The development of a spatially explicit model to estimate radiocaesium body burdens in raccoons (Procyon lotor) for ecological risk assessment

A spatially explicit model of raccoon (Procyon lotor) distribution for the U.S. Department of Energy's (DOE) Savannah River Site (SRS) in west-central South Carolina was developed using data from a raccoon radio-telemetry study and visualized within a Geographic Information System (GIS). An inductive approach was employed to develop three sub-models using the ecological requirements of raccoons studied in the following habitats: (1) man-made reservoirs, (2) bottomland hardwood/riverine systems, and (3) isolated wetland systems. Logistic regression was used to derive probabilistic resource selection functions using habitat compositional data and landscape metrics. The final distribution model provides a spatially explicit probability (likelihood of being in an area) surface for male raccoons. The model is a stand-alone tool consisting of algorithms independent of the specific GIS data layers to which they were derived. The model was then used to predict contaminant burdens in raccoons inhabiting a riverine system contaminated with radiocaesium (137Cs). The predicted 137Cs burdens were less than if one would assume homogeneous use of the contaminated areas. This modelling effort provides a template for DOE managed lands and other large government facilities to establish a framework for site-specific ecological assessments that use wildlife species as endpoints.     

Presence of Enterococcus faecalis in broiler litter and wild bird feces for bacterial source tracking

When Enterococcus faecalis is isolated from fresh feces, its host range appears to be limited to humans and birds. Although E. faecalis is found in human sewage, the extent to which the bacterium is found in broiler litter and in the feces of wild birds is unclear. These results have implications for bacterial source tracking. We determined if media designed for the isolation of fecal enterococci affected this host range, and if E. faecalis was routinely found in broiler litter and in the feces of wild birds. Of five different isolation media, none affected the isolation of E. faecalis. Enterococcus faecalis was routinely found in fresh broiler feces (522 of 1092 isolates; 48%), but rarely in broiler litter (12 of 1452 isolates; <2%). Therefore, broiler litter selects against this bacterium, and broiler litter is an unlikely environmental source of this bacterium. The presence of E. faecalis in eight wild bird species was highly variable. Unless the fecal loading rate from migratory or resident wild birds is high, water samples collected during baseflow conditions with high numbers of E. faecalis may indicate human fecal contamination.     

Locating illicit connections in storm water sewers using fiber-optic distributed temperature sensing

A newly developed technique using distributed temperature sensing (DTS) has been developed to find illicit household sewage connections to storm water systems in the Netherlands. DTS allows for the accurate measurement of temperature along a fiber-optic cable, with high spatial (2 m) and temporal (30 s) resolution. We inserted a fiber-optic cable of 1300 m in two storm water drains. At certain locations, significant temperature differences with an intermittent character were measured, indicating inflow of water that was not storm water. In all cases, we found that foul water from households or companies entered the storm water system through an illicit sewage connection. The method of using temperature differences for illicit connection detection in storm water networks is discussed. The technique of using fiber-optic cables for distributed temperature sensing is explained in detail. The DTS method is a reliable, inexpensive and practically feasible method to detect illicit connections to storm water systems, which does not require access to private property.     

Illicit discharge detection and elimination: Low cost options for source identification and trackdown in stormwater systems

Permit regulations in the U.S. for Municipal Separate Storm Sewer Systems (MS4) require the MS4 to develop a program that detects and eliminates illicit discharges (e.g., improper wastewater connections) into the storm sewer system. Municipalities are interested in cost-effective methods to meet the permit requirements of this federal mandate. Our demonstration project with municipalities in Western New York State evaluated low cost options for illicit discharge trackdown. First, a visual reconnaissance was used to document flowing stormwater outfalls in dry weather. Subsequently, a sampling program was conducted, in combination with decision-making tools, to identify possible sources of illicit discharges. Colorimetric techniques were tested for a number of chemical parameters and the Coliscan Easygel® system was tested for E. coli analysis. Results from these various cost-effective analytical techniques were compared with analysis by standard methods. The E. coli test, in particular, had good precision and was useful in trackdown.     

Seasonal variation in accurate identification of Escherichia coli within a constructed wetland receiving tertiary-treated municipal effluent

As the reuse of municipal wastewater escalates worldwide as a means to extend increasingly limited water supplies, accurate monitoring of water quality parameters, including Escherichia coli (E. coli), increases in importance. Chromogenic media are often used for detection of E. coli in environmental samples, but the presence of unique levels of organic and inorganic compounds alters reclaimed water chemistry, potentially hindering E. coli detection using enzyme-based chromogenic technology. Over seven months, we monitored E. coli levels using m-Coli Blue 24((R)) broth in a constructed wetland filled with tertiary-treated municipal effluent. No E. coli were isolated in the wetland source waters, but E. coli, total coliforms, and heterotrophic bacteria increased dramatically within the wetland on all sampling dates, most probably due to fecal inputs from resident wildlife populations. Confirmatory testing of isolates presumptive for E. coli revealed a 41% rate of false-positive identification using m-Coli Blue 24((R)) broth over seven months. Seasonal differences were evident, as false-positive rates averaged 35% in summer, but rose sharply to 75% in the late fall and winter. Corrected E. coli levels were significantly correlated with electrical conductivity, indicating that water chemistry may be controlling bacterial survival within the wetland. This is the first study to report that accuracy of chromogenic media for microbial enumeration in reclaimed water may show strong seasonal differences, and highlights the importance of validation of microbiological results from chromogenic media for accurate analysis of reclaimed water quality.     

Influence of housing characteristics on bacterial and fungal communities in homes of asthmatic children

Variations in home characteristics, such as moisture and occupancy, affect indoor microbial ecology as well as human exposure to microorganisms. Our objective was to determine how indoor bacterial and fungal community structure and diversity are associated with the broader home environment and its occupants. Next‐generation DNA sequencing was used to describe fungal and bacterial communities in house dust sampled from 198 homes of asthmatic children in southern New England. Housing characteristics included number of people/children, level of urbanization, single/multifamily home, reported mold, reported water leaks, air conditioning (AC) use, and presence of pets. Both fungal and bacterial community structures were non‐random and demonstrated species segregation (C‐score, P < 0.00001). Increased microbial richness was associated with the presence of pets, water leaks, longer AC use, suburban (vs. urban) homes, and dust composition measures (P < 0.05). The most significant differences in community composition were observed for AC use and occupancy (people, children, and pets) characteristics. Occupant density measures were associated with beneficial bacterial taxa, including Lactobacillus johnsonii as measured by qPCR. A more complete knowledge of indoor microbial communities is useful for linking housing characteristics to human health outcomes. Microbial assemblies in house dust result, in part, from the building's physical and occupant characteristics.     

Gross solids from combined sewers in dry weather and storms,elucidating production,storage and social factors

Variation in rates of sanitary hygiene products, toilet tissue and faeces occurring in sewers are presented for dry and wet weather from three steep upstream urban catchments with different economic, age and ethnic profiles. Results show, for example, that total daily solids per capita from the low income and ageing populations are almost twice that from high income or ethnic populations. Relative differences are verified through independent questionnaires. The relationship between solids stored in sewers prior to storms, antecedent dry weather period and the proportion of roof to total catchment area is quantified. A full solids’ flush occurs when storm flows exceed three times the peak dry weather flow. The data presented will assist urban drainage designers in managing pollution caused by the discharge of sewage solids.     

Spatial and temporal variability of ribotyping results at a small watershed in South Carolina

The utility of library-based ribotyping methods for a very small study area was evaluated through comparison of local results to libraries with differing spatial and temporal scales. Ribotyping of Escherichia coli isolates was used to evaluate sources of fecal pollution at a coastal golf course in Beaufort County, South Carolina. Thirty-five E. coli isolates were obtained from water samples from a detention pond for testing against several local and regional libraries of known-source isolate patterns. A library of 92 E. coli ribotype patterns was created from wildlife feces obtained on the site. Additional libraries were available for comparison, including a library from Morgan Island, a small, geographically isolated area (including a monkey colony), and a library from ongoing statewide assessments. Seventeen (49%) of the unknown E. coli isolates matched isolates from raccoon and deer scat from the local library. Two isolates (6%) were matched with monkey sources from Morgan Island, and 13 (37%) were matched to raccoon, deer, and cows from the statewide assessment. Evaluation of repeated ribotyping analyses at the study area revealed evidence of temporal variability of potential sources in the local library. Only one of the isolates from the second year of fecal samples successfully matched with a fecal isolate from the previous year. The results from this study suggest that source identification results were variable both spatially and temporally, and that local, temporally specific libraries are most appropriate for library-based MST studies in small watersheds. Results also suggest that it will be difficult to employ adequate sample sizes to satisfactorily address unknown pattern variability.     

Semi-quantitative evaluation of fecal contamination potential by human and ruminant sources using multiple lines of evidence

Protocols for microbial source tracking of fecal contamination generally are able to identify when a source of contamination is present, but thus far have been unable to evaluate what portion of fecal-indicator bacteria (FIB) came from various sources. A mathematical approach to estimate relative amounts of FIB, such as Escherichia coli, from various sources based on the concentration and distribution of microbial source tracking markers in feces was developed. The approach was tested using dilute fecal suspensions, then applied as part of an analytical suite to a contaminated headwater stream in the Rocky Mountains (Upper Fountain Creek, Colorado). In one single-source fecal suspension, a source that was not present could not be excluded because of incomplete marker specificity; however, human and ruminant sources were detected whenever they were present. In the mixed-feces suspension (pet and human), the minority contributor (human) was detected at a concentration low enough to preclude human contamination as the dominant source of E. coli to the sample. Without the semi-quantitative approach described, simple detects of human-associated marker in stream samples would have provided inaccurate evidence that human contamination was a major source of E. coli to the stream. In samples from Upper Fountain Creek the pattern of E. coli, general and host-associated microbial source tracking markers, nutrients, and wastewater-associated chemical detections—augmented with local observations and land-use patterns—indicated that, contrary to expectations, birds rather than humans or ruminants were the predominant source of fecal contamination to Upper Fountain Creek. This new approach to E. coli allocation, validated by a controlled study and tested by application in a relatively simple setting, represents a widely applicable step forward in the field of microbial source tracking of fecal contamination.     

市政雨、污水管道合槽施工技术的应用

结合某工程实例介绍了市政雨、污水管道合槽施工技术的应用,并与先开挖污水管线、再开挖雨水管线的传统做法进行了比较,对施工进度、质量、成本等指标进行综合比较后表明,在适当条件下,采用合槽施工技术更具合理性.此外,详述了合槽施工的适用条件和施工难点,为工程施工提供了借鉴.     

North Tyneside Bathing Waters' Scheme

The North Tyneside bathing waters' scheme reduces the frequency and quality of storm sewage which is discharged inshore from existing interceptor sewers along the coast.
Flow-simulation modelling is applied to the existing system to determine the frequency and volume of interceptor sewer overflow against a range of storm intensity and return periods. The models are extended to an evaluation of new systems of storm-sewage management.
An optimized solution is described in which new storm sewers are utilized for the storage of all storm-sewage overflow discharges prior to screening and pumping of the storm sewage to sea via an outfall.     

雨污分流改造保留合流管为雨水管的工程实例

在将城市排水合流管改造为雨、污分流管道的过程中，如条件允许可将合流管局部或整体保留用做雨水管，再另外新建污水管道。结合苏州市中心城区完善污水管网工程，介绍了在该市吴县新村内的应用实例。实践表明，保留的合流管能有效地排放正常年份的降雨，甚至是台风暴雨时产生的地面径流。该措施不仅能节约工程投资、缩短工期，还能减少因地面开挖对居民生活造成的不利影响。     

Genotype diversity of Escherichia coli isolates in natural waters determined by PFGE and ERIC-PCR

Most library-dependent bacterial source tracking studies using Escherichia coli (E. coli) have focused on strain diversity of isolates obtained from known human and animal faecal sources for library development. In contrast, this study evaluated the genotype variation of E. coli isolated from natural surface water using pulsed field gel electrophoresis (PFGE) and enterobacterial repetitive intergenic consensus sequence polymerase chain reaction (ERIC-PCR) to better understand these naturally occurring populations. A total of 650 water samples were collected over a nine month period from eleven sampling stations from Lake Waco and Belton Lake in Central Texas. Of the 650 water samples collected, 412 were positive for E. coli, yielding a total of 631 E. coli isolates (1-12 isolates collected per sample). PFGE and ERIC-PCR patterns were successfully generated for 555 isolates and were compared using the curve-based Pearson's product-moment correlation coefficient. The 555 E. coli isolates represented 461 PFGE genotypes, with 84% (386/461) of the genotypes being represented by individual isolates. The remaining 75 genotypes were represented by 2-5 isolates each. Using ERIC-PCR, the 555 E. coli isolates represented 175 genotypes, with 63% (109/175) of the genotypes being represented by individual isolates. In contrast to the PFGE results, two ERIC-PCR genotypes represented 37% of the E. coli isolates, (83 and 124 isolates, respectively), and were found throughout the watersheds both spatially and temporally. Based on the PFGE genotype diversity of water isolates, there is little evidence that a small number of environmentally-adapted E. coli represent dominant populations in the studied waterbodies. However, with the lower discriminatory power technique ERIC-PCR, an opposing conclusion might have been drawn. These results emphasize the importance of considering the resolving power of the source tracking technique being used when assessing strain diversity and geographical stability.     

引导自然的自发秩序——以杭州江洋畈生态 公园内各野生动物种群的环境适应情况为例

都市“再野化”景观的营造,为城市自然栖息地的 存续提供庇护,也为市民近距离观察、欣赏自然全貌提供可 能。以杭州江洋畈生态公园为例,真实还原城市公园的“再野 化”景观设计举措对城市野生动物生存产生的深远影响。基于 园内陆生野生动物的生境分布情况及行为特点,采用样线法和 样方法相结合的方式,对栖息于此的野生动物进行持续观察研 究,发现以下生态设计策略对“引导自然自发秩序”起到至关 重要的作用,有效地促进了当地生态系统的健康演替：1)生境 岛的留存及栈道的设置有利于减少游客对栖息地的干扰;2)降 低灯光亮度、采用LED光源和减少照明时长能有效保护附近鸟 类和昆虫;3)提高果树、蜜源植物和寄主植物的比例能有效 提高鸟类和昆虫的丰富性;4)减少灭虫化学药剂的使用、保 留林间朽木能够显著提升昆虫的多样性,同时为小兽栖息提供 庇护。从园内动物的物种多样性、分布情况及特殊习性的形成 3个方面入手,分析景观设计对野生动物栖息产生的影响,以 及为未来的相关设计提供建议和启发。     

上海市旧住宅小区雨污分流改造效果评价

以上海市两个典型旧住宅区为研究对象,通过对已实施和未实施分流改造小区雨水管道的混接率和旱流水质监测结果的对比,评价了实施小区内部雨、污水管道清理和分流改造措施的效果,为提高城区面源污染控制决策水平提供了依据.     

利用DEM划分雨水汇水面积的方法研究

针对传统的雨水汇水面积划分方法对地形考虑粗略、自动化程度低和人为因素影响大的特点,以城市路网和雨水管线共同组成的排水路线为研究对象,结合数字高程模型(DEM)数据,通过填洼、流向分析及修正、汇水面积划分等方法获得各段排水路线的汇水面积.分析过程突出了地形对汇流的影响,划分准确且自动化程度高.在此基础上可进一步获取各段排水路线上游的汇水面积以及汇水面积特征宽度等重要参数.研究结果可为雨水管网的规划设计及优化分析提供有价值的参考.     

Novel application of a statistical technique, Random Forests, in a bacterial source tracking study

In this study, data from bacterial source tracking (BST) analysis using antibiotic resistance profiles were examined using two statistical techniques, Random Forests (RF) and discriminant analysis (DA) to determine sources of fecal contamination of a Texas water body. Cow Trap and Cedar Lakes are potential oyster harvesting waters located in Brazoria County, Texas, that have been listed as impaired for bacteria on the 2004 Texas 303(d) list. Unknown source Escherichia coli were isolated from water samples collected in the study area during two sampling events. Isolates were confirmed as E. coli using carbon source utilization profiles and then analyzed via ARA, following the Kirby-Bauer disk diffusion method. Zone diameters from ARA profiles were analyzed with both DA and RF. Using a two-way classification (human vs nonhuman), both DA and RF categorized over 90% of the 299 unknown source isolates as a nonhuman source. The average rates of correct classification (ARCCs) for the library of 1172 isolates using DA and RF were 74.6% and 82.3%, respectively. ARCCs from RF ranged from 7.7 to 12.0% higher than those from DA. Rates of correct classification (RCCs) for individual sources classified with RF ranged from 23.2 to 0.2% higher than those of DA, with a mean difference of 9.0%. Additional evidence for the outperformance of DA by RF was found in the comparison of training and test set ARCCs and examination of specific disputed isolates; RF produced higher ARCCs (ranging from 8 to 13% higher) than DA for all 1000 trials (excluding the two-way classification, in which RF outperformed DA 999 out of 1000 times). This is of practical significance for analysis of bacterial source tracking data. Overall, based on both DA and RF results, migratory birds were found to be the source of the largest portion of the unknown E. coli isolates. This study is the first known published application of Random Forests in the field of BST.     

Towards the determination of an optimal scale for stormwater quality management: Micropollutants in a small residential catchment

Stormwater and atmospheric deposits were collected on a small residential urban catchment (0.8 ha) near Paris in order to determine the levels of certain micropollutants (using a preliminary scan of 69 contaminants, followed by a more detailed quantification of PAHs, PCBs, alkylphenols and metals). Atmospheric inputs accounted for only 10%–38% of the stormwater contamination (except for PCBs), thus indicating substantial release within the catchment. On this small upstream catchment however, stormwater contamination is significantly lower than that observed downstream in storm sewers on larger adjacent urban catchments with similar land uses. These results likely stem from cross-contamination activity during transfers inside the sewer system and underscore the advantages of runoff management strategies at the source for controlling stormwater pollutant loads. Moreover, it has been shown that both contamination levels and contaminant speciation evolve with the scale of the catchment, in correlation with a large fraction of dissolved contaminants in upstream runoff, which differs from what has been traditionally assumed for stormwater. Consequently, the choice of treatment device/protocol must be adapted to the management scale as well as to the targeted type of contaminant.     

Potential of tipping flush gate for sedimentation management in open stormwater sewer

This paper presents the design of a tipping flush gate and its potential use in servicing open storm sewers in terms of sedimentation management. The tipping flush gate was installed in a section of open concrete storm sewer located in a commercial area in Taman Pekaka, Nibong Tebal, Penang, Malaysia. Monitoring of the gate operation and performance was done from 14^th November 2012 to 15^th March 2013 covering the beginning and end of the wet season. The sediment profile inside the sewer was measured after each operation of the gate or rainfall event. Results showed that the gate was effective in reducing naturally accumulated sediment in the chosen sewer section. However, proper litter management is needed if the gate is to be implemented in open storm sewer systems. A design guideline for the usage of a tipping flush gate for open storm sewers is also presented in this paper.