Two-wave propagation imaging to evaluate the structure of cancellous bone

The two-wave phenomenon reflects not only bone mass but also the complex bone structure of cancellous bone. We propose a new simple imaging technique based on the two-wave phenomenon for investigating the anisotropic structure of cancellous bone. A cylindrical specimen of cancellous bone was obtained from a bovine femur. The structure (alignment of trabeculae) of the specimen was obtained from 3-D X-ray micro computed tomography imaging. Using a conventional ultrasonic pulse technique, we rotated the receiver around the specimen to investigate the ultrasonic fields after propagation within the specimen. The ultrasonic propagation image clearly showed the effect of the bone structure. We found that the fast wave showed apparent refraction, whereas the slow wave did not. Fast-wave propagation imaging is thus a simple and convenient technique for easy interpretation of the anisotropic structure. This imaging technique has the potential to become a powerful tool to investigate the structure of trabeculae during in vivo measurements.     

Towards optical brain imaging: getting light through a bone

Abstract

Optical imaging and detection in biological samples is severely limited by scattering effects. In particular, optical techniques for measuring conditions beneath the skull and within the bone marrow hold significant promise when it comes to speed, sensitivity and specificity. However, the strong optical scattering due to bone hinders the realization of these methods. In this article, we propose a technique to enhance the transmittance of light through bone. This is achieved by injecting light below the top surface of the bone and utilizing multiple scattering to increase transmittance. This technique suggests that enhancements of 2–6 times may be realized by injection of light 1 mm below the surface of the bone. By enhancing the transmittance of light through bone, we will greatly improve our ability to utilize optical methods to better understand and diagnose conditions within biological media.     

Raman spectroscopic imaging markers for fatigue-related microdamage in bovine bone

Raman spectroscopic markers have been determined for fatigue-related microdamage in bovine bone. Microdamage was induced using a cyclic fatigue loading regime. After loading, the specimens were stained en-bloc with basic fuchsin to facilitate damage visualization and differentiate fatigue-induced damage from cracks generated during subsequent histological sectioning. Bone tissue specimens were examined by light microscopy and hyperspectral near-infrared Raman imaging microscopy. Three regions were defined-tissue with no visible damage, tissue with microcracks, and tissue with diffuse damage. Raman transects, lines of 150-200 Raman spectra, were used for initial tissue surveys. Exploratory factor analysis of the transect Raman spectra has identified spectroscopically distinct chemical microstructures of the bone specimens that correlate with damage. In selected regions of damage, full hyperspectral Raman images were obtained with 1.4-microm spatial resolution. In regions of undamaged tissue, the phosphate nu1 band is found at 957 cm(-1), as expected for the carbonated hydroxyapatic bone mineral. However, in regions of visible microdamage, an additional phosphate nu1 band is observed at 963 cm(-1) and interpreted as a more stoichiometric, less carbonated mineral species. Raman imaging confirms the qualitative relationship between the Raman spectral signature of bone mineral and the type of microdamage in bovine bone. Two tentative explanations for the presence of less carbonated phosphate in damaged regions are proposed.     

Synchrotron imaging reveals bone healing and remodelling strategies in extinct and extant vertebrates

Current understanding of bone healing and remodelling strategies in vertebrates has traditionally relied on morphological observations through the histological analysis of thin sections. However, chemical analysis may also be used in such interpretations, as different elements are known to be absorbed and used by bone for different physiological purposes such as growth and healing. These chemical signatures are beyond the detection limit of most laboratory-based analytical techniques (e.g. scanning electron microscopy). However, synchrotron rapid scanning–X-ray fluorescence (SRS–XRF) is an elemental mapping technique that uniquely combines high sensitivity (ppm), excellent sample resolution (20–100 µm) and the ability to scan large specimens (decimetre scale) approximately 3000 times faster than other mapping techniques. Here, we use SRS–XRF combined with microfocus elemental mapping (2–20 µm) to determine the distribution and concentration of trace elements within pathological and normal bone of both extant and extinct archosaurs (Cathartes aura and Allosaurus fragilis). Results reveal discrete chemical inventories within different bone tissue types and preservation modes. Chemical inventories also revealed detail of histological features not observable in thin section, including fine structures within the interface between pathological and normal bone as well as woven texture within pathological tissue.     

The effects of transducer geometry on artifacts common to diagnostic bone imaging with conventional medical ultrasound

The portability, low cost, and non-ionizing radiation associated with medical ultrasound suggest that it has potential as a superior alternative to X-ray for bone imaging. However, when conventional ultrasound imaging systems are used for bone imaging, clinical acceptance is frequently limited by artifacts derived from reflections occurring away from the main axis of the acoustic beam. In this paper, the physical source of off-axis artifacts and the effect of transducer geometry on these artifacts are investigated in simulation and experimental studies. In agreement with diffraction theory, the sampled linear-array geometry possessed increased off-axis energy compared with single-element piston geometry, and therefore, exhibited greater levels of artifact signal. Simulation and experimental results demonstrated that the linear-array geometry exhibited increased artifact signal when the center frequency increased, when energy off-axis to the main acoustic beam (i.e., grating lobes) was perpendicularly incident upon off-axis surfaces, and when off-axis surfaces were specular rather than diffusive. The simulation model used to simulate specular reflections was validated experimentally and a correlation coefficient of 0.97 between experimental and simulated peak reflection contrast was observed. In ex vivo experiments, the piston geometry yielded 4 and 6.2 dB average contrast improvement compared with the linear array when imaging the spinous process and interlaminar space of an animal spine, respectively. This work indicates that off-axis reflections are a major source of ultrasound image artifacts, particularly in environments comprising specular reflecting (i.e., bone or bone-like) objects. Transducer geometries with reduced sensitivity to off-axis surface reflections, such as a piston transducer geometry, yield significant reductions in image artifact.     

Analysis of micro fracture in human Haversian cortical bone under transverse tension using extended physical imaging

We propose a procedure to investigate local stress intensity factors at the scale of the osteons in human Haversian cortical bone. The method combines a specific experimental setting for a three‐point bending millimetric specimen and a numerical method using the eXtended Finite Element Method (X‐FEM). The interface between the experimental setting and the numerical method is ensured through an imaging technique that analyses the light microscopy observations to import the geometrical heterogeneity of the Haversian microstructures, the boundary conditions and appearing crack discontinuities into the numerical model. The local mechanical elastic Young's moduli are measured by nano‐indentation, and the Poisson ratios are determined by an imaging technique of the stress–strain fields. The model is able to access three scales of measurement: the macro scale of the material level (mm), the micro scale inside the Haversian material for stress–strain fields (10–100µm), and the sub‐micro scale for the crack opening profiles (1–10µm ) and fracture parameters (stress intensity factors). The model is applied to several patients at different aging stages. Copyright © 2009 John Wiley & Sons, Ltd.     

Evaluation of bone matrix and demineralized bone matrix incorporated PLGA matrices for bone repair

The aim of this study was to evaluate the composite matrices prepared using Poly(lactic-co-glycolic acid)- PLGA (85:15) by incorporating human bone matrix (BM) powder or demineralized bone matrix (DBM) powder with the weight ratio of polymer: BM or DBM (75:25) to apply for bone repair. Murine Bone Marrow Stromal Cell (BMSC) attachment was studied with different time points at 30 min, 1 h, 2 h, 4 h, and 6 h for BM/PLGA, DBM/PLGA and PLGA control matrices. All types of matrices were linearly increased the BMSC attachment with the increase of time. Significantly higher number of BMSCs was attached to the both BM/PLGA and DBM/PLGA matrices after 2 h compared to the controls. If BM or DBM is incorporated into biodegradable PLGA matrices and cultured with BMSCs, those composite matrices could be potentially used for bone tissue engineering applications. In addition, particle migration and handling difficulties in DBM powder in clinical applications eliminate using a PLGA matrix. Furthermore, we have observed that DBM/PLGA matrices were structurally stronger compared to the BM/PLGA or control PLGA matrices when they exposed to physiological environment for 72 days.     

Mechanical properties and bone densities of canine trabecular bone

The purposes of this study were (1) to evaluate the mechanical properties of canine epiphyseal cancellous bones from adult canine femoral heads, femoral condyles, tibial plateau, and humeral heads, using indentation and compression tests, and (2) to measure bone densities (apparent density and ash density) of these cancellous bones so as to develop a normal data base of mechanical strength and bone density. The correlations between the two mechanical tests and between these tests and bone densities were also considered. The results showed all of the three mechanical parameters, ultimate load, stiffness, and ultimate strength, measured by the indentation test were higher than those measured by the compression test. Correlation analysis showed that the two sets of mechanical values correlated well (r=0.823–0.952, p<0.01). The apparent density and ash density correlated well with the mechanical parameters determined by the two types of mechanical tests (r=0.737–0.966, p<0.05). © 1998 Chapman & Hall     

Visibility of ghost imaging in a two-arm microscope imaging system

Ghost imaging with a classical thermal source is investigated in a two-arm microscope imaging system. The dependence of the imaging visibility on the aperture of the reference lens is discussed. It is shown that by using large apertures, good visibility as well as enhancing resolution can be obtained. The effects from the distance the object is moved away from the original plane are also studied, and one can obtain good visibility with a well-resolved image by changing the distance.     

Wide field-of-view imaging spectrometer using imaging fiber bundles

A field-of-view-folding approach is proposed to extend the field of view (FOV) of a dispersive imaging spectrometer after introducing several linear arrays of imaging fiber bundles to which to replace the slit. The fiber bundles can flexibly connect fore-optics with a spectrometer to yield an imaging fiber-optic spectrometer (IFOS). The technology of FOV segmenting and folding, which can decrease simultaneously the dimension and spectral distortion of the imaging spectrometer, is described in detail. Because of the sampling function of the fiber bundles, the IFOS is a double-sampling imaging system. We analyze the effect of fiber coupling on the modulation transfer function (MTF) and then develop a cascade MTF model to estimate the imaging performance of the IFOS. A spaceborne IFOS example is presented to describe how the method can be used.     

Ultraviolet and visible imaging and spectrographic imaging instrument

The Ultraviolet and Visible Imaging and Spectrographic Imaging experiment consists of five spectrographic imagers and four imagers. These nine sensors provide spectrographic and imaging capabilities from 110 to 900 nm. The spectrographic imagers share an off-axis design in which selectable slits alternate fields of view (1.00° × 0.10° or 1.00° × 0.05°) and spectral resolutions between 0.5 and 4 nm. Image planes of the spectrographic imager have a programmable spectral dimension with 68, 136, or 272 pixels across each individual spectral band, and a programmable spatial dimension with 5, 10, 20, or 40 pixels across the 1° slit length. A scan mirror sweeps the slit through a second spatial dimension to generate a 1° × 1° spectrographic image once every 5, 10, or 20 s, depending on the scan rate. The four imagers provide narrow-field (1.28° × 1.59°) and wide-field (10.5° × 13.1°) viewing. Each imager has a six-position filter wheel that selects various spectral regimes and neutral densities. The nine sensors ut lize intensified CCD detectors that have an intrascene dynamic range of ~ 10(3) and an interscene dynamic range of ~ 10(5); neutral-density filters provide an additional dynamic range of ~ 10(2-3). The detector uses an automatic gain control that permits the sensors to adjust to scenes of varying intensity. The sensors have common boresights and can operate separately, simultaneously, or synchronously. To be launched aboard the Midcourse Space Experiment spacecraft in the mid-1990's, the ultraviolet and visible imaging and spectrographic imaging instrument will investigate a multitude of celestial, atmospheric, and point sources during its planned 4-yr life.     

Multiaperture imaging

We study the reconstruction of a high-resolution image from multiple low-resolution images by using a nonlinear iterative backprojection algorithm. We exploit diversities in the imaging channels, namely, the number of imagers, magnification, position, rotation, and fill factor, to undo the degradation caused by the optical blur, pixel blur, and additive noise. We quantify the improvements gained by these diversities in the reconstruction process and discuss the trade-off among system parameters. As an example, for a system in which the pixel size is matched to the diffraction-limited optical blur size at a moderate detector noise level, we can reduce the reconstruction root-mean-square error by 570% by using 16 cameras and a large amount of diversity. The algorithm was implemented on a 56 camera array specifically constructed to demonstrate the resolution-enhancement capabilities. Practical issues associated with building and operating this device are presented and analyzed.     

Radar imaging

Radar systems combining coherent signals with frequency and angular diversity offer the possibility of synthesizing images of complex objects with spatial resolution of a few wavelengths. The availability of high-quality microwave sources and components, high-speed digital computers, and efficient signal-processing algorithms allows radar imaging to be implemented in laboratory environments using commercially-available equipment. The paper summarizes fundamental issues by addressing conceptual and practical limits of radar imaging and presents examples obtained from results of measurements in a laboratory environment. Implementation details of sophisticated operational imaging radars are not covered.©1993 John Wiley & Sons Inc     

Intravascular photoacoustic imaging using an IVUS imaging catheter

Catheter-based imaging of atherosclerosis with high resolution, albeit invasive, is extremely important for screening and characterization of vulnerable plaques. Currently, there is a need for an imaging technique capable of providing comprehensive morphological and functional information of plaques. In this paper, we present an intravascular photoacoustic imaging technique to characterize vulnerable plaques by using optical absorption contrast between normal tissue and atherosclerotic lesions. Specifically, we investigate the feasibility of obtaining intravascular photoacoustic (IVPA) images using a high-frequency intravascular ultrasound (IVUS) imaging catheter. Indeed, the combination of IVPA imaging with clinically available IVUS imaging may provide desired functional and morphological assessment of the plaque. The imaging studies were performed with tissue-mimicking arterial vessel phantoms and excised samples of rabbit artery. The results of our study suggest that catheter-based intravascular photoacoustic imaging is possible, and the combination of IVPA with IVUS has the potential to detect and differentiate atherosclerosis based on both the structure and composition of the plaque.