Covered storage reduces losses and improves crop utilisation of nitrogen from solid cattle manure

A 2-year study was carried out to examine the effects of solid cattle manure storage method on (1) total carbon (C) and nitrogen (N) losses, (2) first-year and residual manure dry matter (DM) and N disappearance after litterbag placement on grassland, and (3) apparent herbage N recovery (ANR) after a single surface application to a sandy grassland field. About twelve tonnes of fresh (FRE) manure taken from a litter barn were stored per treatment as stockpiled (STO), composted (COM) and covered (COV) heaps for 130?days, and total C and N losses were estimated. Thereafter, patterns of DM and N disappearance from FRE, COM and COV manures were monitored using litterbags with three mesh sizes (45???m, 1?mm and 4?mm). Herbage ANR from these manures was measured at application rates of 200, 400 and 600?kg?N?ha^?1. During the storage period, only about 10?% of the initial N_total was lost from the COV heap, whereas these losses were 31?% from the STO heap and 46?% from the COM heap. The respective C_total losses were 17, 59 and 67?%. After field placement, overall manure DM and N disappearance rates from all mesh sizes of the litterbags were in the order: COV?>?FRE?>?COM (P?<?0.05). Independent of N application rate, total herbage ANR was the highest from COV and the lowest from COM manure over two growing seasons (23 vs. 14?%; P?<?0.05). Including the N losses during storage, an almost three times higher herbage ANR (20 vs. 7?%) of the manure N taken from the barn was observed by using COV versus COM manure. In case of FRE manure this ANR fraction was 17?%. It is concluded that COV storage reduced storage C and N losses to a minimum. After field application, manure stored under this method decomposed faster and more N was available for plant uptake, especially when compared to COM manure.     

Interactions of aerobically decomposed cattle manure and nitrogen fertilizer applied to soil

Inorganic N fertiliser may be applied to soil in addition to cattle manure by smallholder farmers in developing countries: (a) to complement fertilization; (b) to control a possible immobilisation of N by the manure; and (c) to eliminate the risk of yield depression due to lack of plant available N. The aim of this study was to find out if and how much N was immobilised by cattle manure, if and when remineralisation of N will take place and, if added N has an effect on decomposition of cattle manure in soil. A laboratory study was conducted applying inorganic N fertiliser to soil (NH4NO3 equivalent to 30, 60 and 120 kg N ha-1) together with four cattle manures with different C/N ratios (9–18). CO2–C mineralisation and changes of inorganic N in soil were determined over 60 d. Immobilisation of fertiliser N occurred with manure having the lowest C/N ratio but not with the manures having a higher C/N ratios. Maximum immobilization of fertiliser N (23–36%) occurred within 21 d and thereafter N was mineralised. Carbon dioxide evolution decreased in cattle manure-amended soil at increasing rates of N fertiliser, but decomposition was still higher than from the unamended control. None of the manure treated soils had significantly different contents of inorganic N after 2 months of incubation. It was not possible to use the C/N ratio of aerobically decomposed cattle manure as a tool to predict mineralization or immobilization of N. It was concluded that aerobically decomposed solid cattle manures do not contribute to the N supply of crops in the short term but can immobilize fertiliser N applied at the same time.     

Modeling nitrogen leaching from Andosols amended with different composted manures using LEACHM

Andosols, distributed widely around the Pacific basin, have unique soil–water and solute transport properties because of their stepwise water retention curves and high anion-adsorption capacity. The model modification and verification for these properties are crucial for evaluating the potential for improved agricultural management (e.g., using organic matter instead of inorganic fertilizer) to reduce N loss from the soils. Here, we improved an existing biogeochemical model, LEACHM, to predict long-term N leaching from Andosols amended with composted manure, without optimization to fit measured field data. The modified model was verified by observations from a 5.6-year lysimeter experiment with different rates of inorganic N fertilizer plus composted manure (100 + 0, 75 + 25, or 25 + 75%) of two different types (cattle, swine) on lettuce, sorghum (as a catch crop), and Chinese cabbage in rotation. Incorporation of Durner’s bimodal model dramatically improved predictions of drainage water volume and evapotranspiration. The non-linear Langmuir adsorption isotherms for soil NH₄ ⁺ and NO₃ ^? improved model performance in simulating crop N uptake and N leaching loss. The RMSE, R², and index of Agreement were evaluated as satisfactory in all lysimeters. Our model explained reasonably well that improved agricultural management decreased in current available N addition rates by 8.82–35.6% and reduced in the yearly averaged NO₃ leaching by 8.70–41.8%. A modified model relating soil hydraulic properties and N adsorption properties could thus accurately predict N leaching under different long-term N application rates/types, and could be useful for supporting agricultural management decisions in cropped Andosols.     

Evaluation of methods of measurement of nitrogen in poultry and animal manures

Kjeldahl nitrogen (N), total N and forms of inorganic N (ammoniacal (NH₄)-N, nitrate (NO₃)-N and nitrite (NO₂)-N) were measured in a range of animal manures. The manures include fresh samples of poultry manure, sheep manure, horse manure, dairy slurry and pig slurry and composted poultry manure. Kjeldahl N was measured by standard micro-Kjeldahl digestion. For total N measurements, NO₃-N and NO₂-N were recovered during Kjeldahl digestion by pretreatments with various oxidizing and reducing agents. Inorganic forms of N were measured by extraction with 2M KCl solution.Kjeldahl digestion alone allowed measurement only of organic N and NH₄-N. Amongst various modifications to the Kjeldahl, pretreatment with either acidified (H₂SO₄) Zn-CrK(SO₄)₂ or acidified (H₂SO₄) reduced Fe achieved complete recovery of NO₃-N. Nitrite N was only recovered by first oxidising the NO ₂ ^- to NO ₃ ^- with KMnO₄ followed by reduction to NH₄-N with acidified (H₂SO₄) reduced Fe.More than 95% of the total N in fresh animal manure was present as organic N and NH₄-N which were recovered by the standard Kjeldahl digestion. In the case of fresh manures there was no difference between the amount of total N measured by the Kjeldahl digestion and its modified methods. However composting of poultry manure or drying of poultry manure, pig slurry and dairy slurry resulted in an increase in NO₃-N which was not recovered during Kjeldahl digestion alone. Under these conditions the total N could be measured by pretreating the samples with KMnO₄ and reduced Fe prior to Kjeldahl digestion.Drying of animal manures caused a decrease in organic N and NH₄-N, especially in poultry, pig and dairy manures. There was a slight increase in NO₃-N; but most of the decrease in N content with drying was attributed to the volatilization loss of ammonia (NH₃). Amongst various drying methods examined air drying caused maximum loss of N as NH₃ whereas freeze drying caused minimum loss of N. This suggests that fresh animal manures can be freeze dried for analysis of N which causes minimum loss of N.     

Unconventional feeds for small ruminants in dry areas have a minor effect on manure nitrogen flow in the soil–plant system

In dry areas, unconventional feeds are increasingly used for mitigating feed shortages and rangeland degradation. We evaluated how feeding sheep diets containing olive leaves, saltbush leaves and olive cake affects manure quality compared to a barley straw based diet. Soil incubation and plant growth experiments were carried out to measure soil nitrogen (N) mineralization and N uptake by barley plants and to calculate N flow through the feed-animal-soil–plant system. Fresh feces, composts consisting of feces, urine and straw, and ammonium sulfate fertilizer were mixed with soil at rate of 90 mg N kg^?1 soil dry matter. Comparisons were made with non-amended soils (control) and soils amended with fresh olive cake applied at 90 and 22.5 mg N kg^?1 soil dry matter, respectively. The latter treatment enabled investigation of the effect of passage of olive cake through the digestive tract of sheep on N availability and phenol transformation. Applying fresh olive cake and feces, except the saltbush leaf derived feces, resulted in a net N immobilization. All composts resulted in net N mineralization, although not significantly different from the 0N control soil. Barley growing in soils with amendment that caused N immobilization took up less N than barley growing on the 0N treatment. Reduction in N uptake was most pronounced after amendment with fresh-olive cake. Treatments with net mineralization increased barley N uptake over the 0N treatment with 2–16 % of N applied being taken up. Dietary composition had a minor effect on N fertilizer value of either feces or compost, but feces N alone was not an efficient N source.     

Predicting nitrogen leaching with the modified LEACHM model: validation in soils receiving long-term application of animal manure composts

A variety of process-based models have been developed for predicting nitrogen (N) dynamics in agro-ecosystem; however, no reliable models have been validated for N leaching from soils receiving a long-term application of different types of animal manure composts. The Leaching Estimation and Chemistry Model (LEACHM) was recently modified by incorporating the basic structure of Rothamsted Carbon Model for extending its ability to describe soil organic matter decomposition and subsequent N leaching in soils rich in organic matter. We evaluate the applicability of the modified LEACHM in cropped Yellow soils receiving 10-year application of cattle or swine manure compost in addition to chemical fertilizers, where high-frequency field monitoring data of soil water contents, soil N contents and leachate N concentrations were available for the last 3 years. Particular attention was paid to determine all input parameters from independent measurements, parameterization from known soil properties or databases without optimisation to fit the measured field data. The model reasonably predicted temporal changes in the soil NH₄-N and NO₃-N contents, and inorganic N concentrations in the leachate as well as their differences due to different manure compost/chemical fertilizer applications. The simulations of leached N concentration yielded a Willmott index of agreement (IA) of 0.62–0.68, with those for soil moisture, soil nitrate content and crop N uptake all within an acceptable IA range. In view of the good performance without site-specific calibrations, the modified LEACHM appears to be a valuable tool for predicting N leaching from cropped soils receiving long-term manure compost applications.     

Crop yield, nitrogen uptake and nitrate-nitrogen accumulation in soil as affected by 23 annual applications of fertilizer and manure in the rainfed region of Northwestern China

Application of chemical fertilizers and farmyard manure affects crop productivity and improves nutrient cycling within soil–plant systems, but the magnitude varies with soil-climatic conditions. A long-term (1982–2004) field experiment was conducted to investigate the effects of nitrogen (N), phosphorus (P), and potassium (K) fertilizers and farmyard swine manure (M) on seed and straw yield, protein concentration, and N uptake in the seed and straw of 19-year winter wheat (Triticum aestivum L.) and four-year oilseed (three-year canola, Brassica napus L. in 1987, 2000 and 2003; one-year flax, Linum usitatisimum L. in 1991), accumulation of nitrate-N (NO₃-N) in the soil profile (0–210 cm), and N balance sheet on a Huangmian soil (calcaric cambisols, FAO) near Tianshui, Gansu, China. The two main plot treatments were without and with farmyard swine manure (M); sub-plot treatments were control (Ck), N, NP, and NPK.␣The average seed yield decreased in the order MNPK ≥ MNP > MN ≥ NPK ≥ NP > M > N > Ck. The average effect of manure and fertilizers on seed yield was in the order M > N > P > K. The seed yield increase was 20.5% for M, 17.8% for N, 14.2% for P, and 2.9 % for K treatment. Seed yield response to fertilizers was much greater for N and P than for K, and it was much greater for no manure than for manure treatment. The response of straw yield to fertilization treatments was usually similar to that of seed yield. The N fertilizer and manure significantly increased protein concentration and N uptake plant. From the standpoint of increasing crop yield and seed quality, MNPK was the best fertilization strategy. Annual applications of N fertilizer and manure for 23 successive years had a marked effect on NO₃-N accumulation in the 0–210 cm soil profile. Accumulation of NO₃-N in the deeper soil layers with application of N fertilizer and manure is regarded as a potential danger, because of pollution of the soil environment and of groundwater. Application of N fertilizer in combination with P and/or K fertilizers reduced residual soil NO₃-N significantly compared with N fertilizer alone in both no manure and manure plots. The findings suggest that integrated and balanced application of N, P, and K fertilizers and␣manure at proper rates is important for protecting soil and groundwater from potential NO₃-N pollution and for maintaining high crop productivity in the rainfed region of Northwestern China.     

Determination of the mineralization of nitrogen from composted chicken manure as affected by temperature

Incubations at 5, 10 or 20°C of composted chicken manure in a sand, clay or loam soil consistently released nitrogen. A statistical model fitted to the data confirmed that the temperature dependence followed an Arrhenius pattern. The data were used to test the hypothesis that composted manure behaves more like native soil organic matter than fresh residues and this idea is illustrated with the use of a computer simulation model. Extrapolation of the model results in several climates suggests that about 40% of the organic N in composted chicken manure becomes available to crops during the first year and 6–12% during each subsequent year.     

Stoichiometry of animal manure and implications for nutrient cycling and agriculture in sub-Saharan Africa

A number of studies have recommended application of large quantities of manure alone or in combination with inorganic fertilizer in sub-Saharan Africa (SSA). However, yield responses of cereals such as maize are very modest even at manure application rates exceeding 10 t ha^?1 year^?1. We conducted a meta-analysis of data from 64 studies across 14 countries in SSA in order to explore variability in nutrient concentrations, stoichiometry and maize yield responses to animal manure. We observed novel instances of stoichiometry and correlations between organic carbon (C), total nitrogen (N), phosphorus (P) and potassium (K) concentrations, and elemental ratios in manure. In 27% of the manure samples the C:N ratio was greater than 25 indicating that N will be potentially unavailable to crops due to net immobilization. In over 94% of the manure samples, the N:P and C:P ratios were <15 and <200 indicating net P mineralization. Therefore, decomposition rates and crop responses are likely to be N-limited rather than P-limited in the majority of the manure applied. Our analyses also demonstrate that manure application rates and N and P concentrations are less important than C:N and N:P ratios in determining maize yield response to manure. Therefore, emphasis in the future should not be on increasing manure application rates but on approaches that ensure favourable C:N and N:P ratios. Our findings also suggest the need for feeding animals with high quality diet to get better quality manure, higher crop yields and improve household food security in SSA.     

Application of liquid cattle manure and inorganic fertilizers affect dry matter,nitrogen accumulation,and partitioning in maize

Efficient use of N applied in the form of organic and inorganic fertilizers is important in maize (Zea mays L.) production to maximize producer’s economic returns and maintain soil and water quality. A field study was conducted for three consecutive years (2003–2005) in Thessaloniki, Greece to investigate whether liquid cattle manure can be used to replace inorganic fertilizers and also whether inorganic fertilizer can be applied preplant or as a combination of preplant and sidedress and can affect maize growth, development and N use efficiency. The treatments were control (unfertilized), liquid dairy cattle manure (Manure), application of 260 kg N ha⁻¹ year⁻¹ as basal dressing (N-single), application of 130 kg ha⁻¹ year⁻¹ N as basal dressing before sowing and 130 kg N ha⁻¹ when plants were at the eight-leaf stage (V8) (N-split). In 2 out of the 3 years of the study there was a significant positive effect of fertilizer application on maize growth, development, N uptake, and partitioning compared with the control. Dry matter production was increased by an average of 39% during the 2 years in plots fertilized either with manure or inorganic fertilizers than the control plots. Also from the yield components kernel weight per ear and number of kernels per ear were increased by an average of 35% and 32%, respectively in the fertilized plots compared with the control plots. Chlorophyll level was affected as it was increased by an average of 18%, 14%, and 18% at the ten-leaf stage (V10), silking and milk stage, respectively in the fertilization treatments compared with the control. Similar trend was observed in the other parameters that were studied. No differences were found between the manure and the different times of N application which indicates that manure can be used to replace inorganic fertilizer. Applying N either preplant in a single application or in split application (half of N preplant and half as sidedress) did not have any effect on any characteristics that were studied indicating that preplant application can be used as it is more cost effective. The present study indicates that liquid cattle manure can be used to replace inorganic fertilizers and also that there was no difference between preplant and sidedress application of N.     

Manure and fertilizer contributions to soil mineral nitrogen and the yield of forage maize

Fifteen field trials were conducted to evaluate soil mineral N measurement as a means for quantifying the total N supply to forage maize and so to form the basis for fertilizer recommendations on a crop-specific basis. In every trial, 4 rates of cattle manure N (nominally 0, 80, 160, 240 kg N per ha) and 4 rates of ammonium nitrate (0, 50, 100, 150 kg N per ha) were factorially combined. Soil mineral N measurements were made before manure application, at the time of maize drilling, 7-10 weeks after drilling and after harvest. Measurements on control treatments which received no manure or ammonium nitrate showed extensive net mineralisation of soil N (mean 140 kg N per ha) in the 7-10 weeks after drilling followed by a decrease due to crop uptake, and probably net immobilisation, of approximately the same amount by harvest. This net mineralisation was probably the reason why only one trial showed a significant dry-matter yield response to ammonium nitrate. Results indicated that , to be useful for N recommendations, soil mineral N measurements should be taken 7-10 weeks after drilling. Only if the amount of mineral N at this time is less than expected crop N offtake should fertilizer N be applied. A mean of around 64% of the N applied in ammonium nitrate could be accounted for in soil mineral N after harvest of the maize, although this was reduced to 24% in the single trial where a dry-matter response to ammonium nitrate was recorded.     

Net N immobilisation during the biodegradation of mucilage in soil as affected by repeated mineral and organic fertilisation

Repeated fertilisation of crops with manure commonly increases both the amount and the quality of soil organic matter, the mineralisation of which strongly determines the availability of N to plants. In the rhizosphere, the microbial mineralisation/immobilisation of N is enhanced due to rhizodeposition (release of organic compounds from roots). In this work, we studied N transformations during incubation of maize root mucilage in soils that had been previously fertilized with composted pig manure or with ammonium nitrate for 7 years. Our work revealed mucilage was rapidly mineralised (average half-life of 3 days), inducing a rapid N immobilisation of 94 mg N g⁻¹ of mucilage C, followed by a slow remineralisation. Fertilisation with manure induced a persistent stimulation of the soil organic matter mineralisation, leading to an enhanced content of soil inorganic N (+23% in 58 days of incubation). Due to this stimulation of microbial activity in the soil fertilized with manure, the decrease in inorganic N during the biodegradation of mucilage lasted a shorter time as compared to the mineral fertilisation. However, the type of fertilisation did not significantly change the amount of N immobilised.     

Nitrogen leaching and crop availability in manured catch crop systems in Sweden

Results are presented from five years (1990–1995) of a field leaching experiment on a sandy soil in south-west Sweden. The aim was to study N leaching, change in soil organic N and N mineralization in cropping systems with continuous use of liquid manure (two application rates) and catch crops. N leaching from drains, N uptake in crops and mineral N in the soil were measured. Simulation models were used to calculate the N budget and N mineralization in the soil and to make predictions of improved fertilization strategies in relation to manure applications and changing the time for incorporation of catch crops. In treatments without catch crops, a normal and a double application of manure increased average N leaching by 15 and 34%, respectively, compared to treatment with commercial fertilizer. Catch crops reduced N leaching by, on average, 60% in treatments with a normal application of manure and commercial fertilizer, but only by 35% in the treatment with double the normal application rate of manure. Incorporation of catch crops in spring increased simulated net N mineralization during the crop vegetation period, and also during early autumn. In conclusion, manured systems resulted in larger N leaching than those receiving commercial fertilizer, mainly due to larger applications of mineral N in spring. More careful adaptation of commercial N fertilization with respect to the amounts of NH₄-N applied with manure could, according to the simulations, reduce N leaching. Under-sown ryegrass catch crops effectively reduced N leaching in manured systems. Incorporating catch crop residues in late autumn instead of spring might be preferable with respect to N availability in the soil for the next crop, and would not increase N leaching.     

Use ofLeucaena leucocephala (Lam. de Wit) leaves as a nitrogen source for crop production

Field and pot trials were conducted to determine optimum management practices for usingLeucaena leucocephala (Lam. de Wit) leaves as a N source for crop production. Field trials with maize showed no benefit from split application of leucaena leaves or from application of fresh as opposed to dried material. Field trials also failed to show any difference between incorporation as opposed to surface application of leucaena leaves. This may be attributed to the low nitrogen response observed. Pot trials however, showed that soil incorporation of leucaena leaves was more effective than surface application in increasing plant dry weight.Leucaena leaves were not as effective as inorganic N in increasing maize grain yield (field trials) or dry matter production (pot trial). Unlike inorganic N, leucaena leaves had a significant residual effect on the succeeding maize crop. In decomposition studies, buried leucaena leaves decomposed more quickly than surface-applied leaves, and fresh leaves decomposed more rapidly than dried leaves.     

How winter cover crops and tillage intensities affect nitrogen availability in eggplant

This study evaluates the fate of nitrogen (N) content in winter cover crops under different tillage intensities. Field trials were conducted over a 2-year period in a Mediterranean environment adopting a cover crop–eggplant sequence. The treatments were: three cover crops (hairy vetch, oat and oilseed rape); three tillage intensities (residue left on soil surface, shallow green manure and deep green manure). The measurements included: cover crop and eggplant characteristics, N mineralization from cover crops, soil inorganic N and soil CO₂ emission. At cover crop termination, N accumulated in the cover crops was 207, 77 and 77 kg N ha^?1 in hairy vetch, oat and oilseed rape, respectively. Tillage intensity affected biomass decomposition and N mineralization from cover crop residues which were slower when residues were left on soil surface (54 and 71%, respectively) than when incorporated into the soil (66 and 79%, respectively). Hairy vetch showed a greater ability to supply N to eggplant (151 kg N ha^?1), due to the fast decay of its residues, consequently, the N balance index was always high after hairy vetch throughout eggplant cultivation. N mineralized by cover crops was positively correlated with total soil CO₂ emission and soil inorganic N. Placing cover crop residues on soil surface enhances synchronization between N mineralized and eggplant N demand in hairy vetch, while in oat it appears to mitigate the shortage of soil inorganic N for the following vegetable. These findings may also be extended to other summer vegetables which have similar requirements to the eggplant.     

Grazing intensity effects on soil nitrogen mineralization in semi-arid grassland on the Loess Plateau of northern China

Soil nitrogen transformation has been the subject of growing attention in many semi-arid grassland ecosystems. In our study, we employed an intact soil core in situ incubation technique and measured seasonal changes in soil net nitrogen mineralization and nitrification rates. The measurements were taken from the upper 0–10 cm soil layer of a permanent grassland during a growing season in a 8.5-year field experiment on the Loess Plateau, China that had four grazing intensities (0, 2.7, 5.3 and 8.7 sheep ha⁻¹). Our results demonstrate marked seasonal variations in inorganic nitrogen pools, net nitrogen mineralization and net nitrification. The rates of mineralization and nitrification were highest in August and lowest in September. No consistent differences in monthly net nitrogen mineralization and monthly nitrification rates were observed among the different grazing intensities. Sheep grazing stimulated nitrogen transformation, and the most stimulation occurred at a heavy grazing intensity of 8.7 sheep ha⁻¹. The mean soil net nitrification rate was positively correlated with the soil C/N ratio and pH. The mean N mineralization rate was negatively correlated with soil organic carbon, but was positively correlated with the soil C/N ratio. Our study demonstrated net nitrogen mineralization and nitrification rates were strongly linked to grazing intensity, soil temperature and moisture content.     

Recycling of nutrients in Japanese mint - assessment of soil fertility and crop yield

The efficiency of mint-residue, composted alone and amended with starter nutrients, microbial culture and soil suspension (hereafter termed amended compost) was compared with farm yard manure and inorganic fertilizer on the yield of Japanese mint (Mentha arvensis L.) and improvement of soil fertility. Herbage, essential oil yield, nutrient uptake of Japanese mint and soil available nutrients were significantly enhanced due to application of amended compost as compared to nonamended compost, farm yard manure and inorganic fertilizer. Organic fertilized soils maintained significantly higher available nutrients throughout the crop growth period as compared to inorganic fertilized soils. No additional improvement in yields and soil fertility was recorded with combined application of compost and inorganic fertilizer in 1:1 ratio as against addition of compost alone. Advantage of such combinations was recorded in case of farm yard manure. Results of the study suggested possibilities for nutrient recycling through composted mint-residue for supplementing the fertilizers requirement of Japanese mint.     

Influence of different manuring systems with and without biogas digestion on soil organic matter and nitrogen inputs,flows and budgets in organic cropping systems

Nitrogen (N) and carbon (C) cycles are closely linked in organic farming systems. Use of residues for biogas digestion may reduce N-losses and lead to higher farmland productivity. However, digestion is connected to large losses of organic C. It is the purpose of this paper (1) to compare farming systems based on liquid slurry and solid farmyard manure regarding the N, C and organic dry matter (ODM) inputs and flows, (2) to analyse the effect of digestion on soil N, C and ODM inputs and flows within the cropping system, (3) to assess the effects of organic manure management on biological N₂ fixation (BNF), and (4) to assess the effect of biogas digestion on the sustainability of the cropping systems in terms of N and C budgets. The BNF by clover/grass-leys was the most important single N input, followed by the BNF supplied by legume cover cropping. Growth of crops in organic farming systems is very often N limited, and not limited by the soil C inputs. However, balances of N inputs showed that the implemented organic farming systems have the potential to supply high amounts of N to meet crop N demand. The level of plant available N to non-legume main crops was much lower, in comparison to the total N inputs. Reasons were the non-synchronized timing of N mineralization and crop N demand, the high unproductive gaseous N losses and an unfocussed allocation in space and time of the circulating N within the crop rotation (e.g. allocation of immobile manures to legumes or of mobile manures to cover crops). Simultaneously, organic cropping systems very often showed large C surpluses, which may be potentially increased the N shortage due to the immobilization of N. Soil organic matter supply and soil humus balance (a balance sheet calculated from factors describing the cultivation effects on humus increasing and humus depleting crops, and organic manure application) were higher in cropping systems based on liquid slurry than in those based on solid farmyard manure (+19%). Simultaneously, soil N surplus was higher due to lower gaseous N losses (+14%). Biogas digestion of slurry had only a very slight effect on both the soil N and the soil C budget. The effect on the N budget was also slight if the liquid slurry was stored in closed repositories. Digestion of residues like slurry, crop residues and cover crops reduced in a mixed farming system the soil C supply unilaterally (approximately −33%), and increased the amounts of readily available N (approximately +70–75%). The long-term challenge for organic farming systems is to find instruments that reduce N losses to a minimum, to keep the most limiting fraction of N (ammonia-N) within the system, and to enhance the direct manuring effect of the available manures to non-legume main crops.     

Seasonal nitrogen availability from current and past applications of manure

Proper management of manure nitrogen (N) requires the ability to match the rate and extent of manure N availability with crop needs. This includes recognizing the potential importance of N contributions from residual manure N that accumulates with repeated applications. Nitrogen availability relative to barley needs was assessed in plots with 13–16 years continuous histories of contrasting manure-based (solid-bedded beef) and fertilizer-based soil treatments in the Maine Potato Ecosystem Project. Soil and barley samples were collected every 7–14 days during 2003–2005, and once in 2006. Barley dry matter and N content were equivalent between the two systems. In the manure-based system, temporal patterns of N availability were more synchronous with early season crop needs than in the fertilizer-based system, but continued mineralization after harvest was also observed. In 2004–2006, samples were collected from subplots where manure/fertilizer was withheld to estimate the proportion of available N originating from current versus previous manure applications. Apparent N recovery of current years’ applications of manure organic N was 8–11% and less than predicted by a standard decay series model for beef manure (25%), highlighting the need to adjust manure N credits for crops with shorter growing seasons and lower N uptake capacities than corn. The relative contribution of residual manure N to total manure N uptake was greater than predicted from the decay series model, providing support for a residual N effect from repeated manure applications that is not accounted for in standard manure recommendations.     

Differences in Yields,Residue Composition and N Mineralization Dynamics of Bt and Non-Bt Maize

Cultivation of genetically modified crops may have several direct and indirect effects on soil ecosystem processes, such as soil nitrogen (N) transformations. Field studies were initiated in Northeast Missouri in 2002 and 2003 to determine grain and biomass yields and the effects of application of crop residues from five Bt maize hybrids and their respective non-Bt isolines on soil inorganic N under tilled and no-till conditions in a maize-soybean rotation. A separate aerobic incubation study examined soil N mineralization from residue components (leaves, stems, roots) of one Bt maize hybrid and its non-Bt isoline in soils of varying soil textural class. Three Bt maize hybrids produced 13–23% greater grain yields than the non-Bt isolines. Generally no differences in leaf and stem tissues composition and biomass was observed between Bt and non-Bt maize varieties. Additionally, no differences were observed in cumulative N mineralization from Bt and non-Bt maize residues, except for non-Bt maize roots that mineralized 2.7 times more N than Bt maize roots in silt loam soil. Incorporation of Bt residues in the field did not significantly affect soil inorganic N under tilled or no-till conditions. Overall Bt and non-Bt maize residues did not differ in their effect on N dynamics in laboratory and field studies.