共查询到20条相似文献，搜索用时 15 毫秒
1.
Chang Sup Kim Yoo Seong Choi Wooree Ko Jeong Hyun Seo Jieun Lee Hyung Joon Cha 《Advanced functional materials》2011,21(21):4100-4100
2.
Chang Sup Kim Yoo Seong Choi Wooree Ko Jeong Hyun Seo Jieun Lee Hyung Joon Cha 《Advanced functional materials》2011,21(21):4101-4108
The efficient immobilization of antibodies onto solid surfaces is vital for the sensitivity and specificity of various immunoassays and immunosensors. A novel linker protein, BC‐MAP, is designed and produced in Escherichia coli by genetically fusing mussel adhesive protein (MAP) with two domains (B and C) of protein A (antibody‐binding protein) for efficient antibody immobilization on diverse surfaces. Through direct surface‐coating analyses, it is found that BC‐MAP successfully coats diverse surfaces including glass, polymers, and metals, but the BC domain alone does not. Importantly, antibodies are efficiently immobilized on BC‐MAP‐coated surfaces, and the immobilized antibodies interact selectively with their corresponding antigen. Quartz‐crystal‐microbalance analyses show that BC‐MAP has excellent antibody‐binding ability compared to that of BC protein on gold surfaces. These results demonstrate that the MAP domain, with uniquely strong underwater adhesive properties, plays a role in the direct and efficient coating of BC‐MAP molecules onto diverse surfaces that lack additional surface treatment, and the BC domain of BC‐MAP contributes to the selective and oriented immobilization of antibodies on BC‐MAP‐coated surfaces. Thus, the BC‐MAP fusion protein could be a valuable novel linker material for the facile and efficient immobilization of antibodies onto diverse solid supports. 相似文献
3.
Anderson TH Yu J Estrada A Hammer MU Waite JH Israelachvili JN 《Advanced functional materials》2010,20(23):4196-4205
Mussels use a variety of 3, 4-dihydroxyphenyl-l-alanine (DOPA) rich proteins specifically tailored to adhering to wet surfaces. Synthetic polypeptide analogues of adhesive mussel foot proteins (specifically mfp-3) are used to study the role of DOPA in adhesion. The mussel-inspired peptide is a random copolymer of DOPA and N(5) -(2-hydroxyethyl)-l-glutamine synthesized with DOPA concentrations of 0-27 mol% and molecular weights of 5.9-7.1 kDa. Thin films (3-5 nm thick) of the mussel-inspired peptide are used in the surface forces apparatus (SFA) to measure the force-distance profiles and adhesion and cohesion energies of the films in an acetate buffer. The adhesion energies of the mussel-inspired peptide films to mica and TiO(2) surfaces increase with DOPA concentration. The adhesion energy to mica is 0.09 μJ m(-2) mol(DOPA) (-1) and does not depend on contact time or load. The adhesion energy to TiO(2) is 0.29 μJ m(-2) mol(DOPA) (-1) for short contact times and increases to 0.51 μJ m(-2) mol(DOPA) (-1) for contact times >60 min in a way suggestive of a phase transition within the film. Oxidation of DOPA to the quinone form, either by addition of periodate or by increasing the pH, increases the thickness and reduces the cohesion of the films. Adding thiol containing polymers between the oxidized films recovers some of the cohesion strength. Comparison of the mussel-inspired peptide films to previous studies on mfp-3 thin films show that the strong adhesion and cohesion in mfp-3 films can be attributed to DOPA groups favorably oriented within or at the interface of these films. 相似文献
4.
Mussel‐Inspired Adhesive and Conductive Hydrogel with Long‐Lasting Moisture and Extreme Temperature Tolerance 
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下载免费PDF全文 Lu Han Kezhi Liu Menghao Wang Kefeng Wang Liming Fang Haiting Chen Jie Zhou Xiong Lu 《Advanced functional materials》2018,28(3)
Conductive hydrogels are a promising class of materials to design bioelectronics for new technological interfaces with human body, which are required to work for a long‐term or under extreme environment. Traditional hydrogels are limited in short‐term usage under room temperature, as it is difficult to retain water under cold or hot environment. Inspired by the antifreezing/antiheating behaviors from nature, and based on mussel chemistry, an adhesive and conductive hydrogel is developed with long‐lasting moisture lock‐in capability and extreme temperature tolerance, which is formed in a binary‐solvent system composed of water and glycerol. Polydopamine (PDA)‐decorated carbon nanotubes (CNTs) are incorporated into the hydrogel, which assign conductivity to the hydrogel and serve as nanoreinforcements to enhance the mechanical properties of the hydrogel. The catechol groups on PDA and viscous glycerol endow the hydrogel with high tissue adhesiveness. Particularly, the hydrogel is thermal tolerant to maintain all the properties under extreme wide tempreature spectrum (?20 or 60 °C) or stored for a long term. In summary, this mussel‐inspired hydrogel is a promising material for self‐adhesive bioelectronics to detect biosignals in cold or hot environments, and also as a dressing to protect skin from injuries related to frostbites or burns. 相似文献
5.
Light‐Emitting Diodes: Micropatterned Down‐Converting Coating for White Bio‐Hybrid Light‐Emitting Diodes (Adv. Funct. Mater. 1/2017) 下载免费PDF全文
下载免费PDF全文 Lukas Niklaus Samira Tansaz Haider Dakhil Katharina T. Weber Marlene Pröschel Martina Lang Monika Kostrzewa Pedro B. Coto Rainer Detsch Uwe Sonnewald Andreas Wierschem Aldo R. Boccaccini Rubén D. Costa 《Advanced functional materials》2017,27(1)
6.
Lukas Niklaus Samira Tansaz Haider Dakhil Katharina T. Weber Marlene Pröschel Martina Lang Monika Kostrzewa Pedro B. Coto Rainer Detsch Uwe Sonnewald Andreas Wierschem Aldo R. Boccaccini Rubén D. Costa 《Advanced functional materials》2017,27(1)
White hybrid light‐emitting diodes (WHLEDs) are considered as a solid approach toward environmentally sustainable lighting sources that meet the “Green Photonics” requirements. Here, WHLEDs with protein‐based down‐converting coatings, i.e., Bio‐WHLEDs, are demonstrated and exhibit worthy white color quality, luminous efficiency, and stability values. The coatings feature a multilayered cascade‐like architecture with thicknesses of 1–3 mm. This limits the efficiency due to the low optical transmittance. Thus, submillimeter coatings, where the location of the proteins is well‐defined, are highly desired. It is in this context where the thrust of this work sets in. Here, a straightforward way to design microstructured single‐layer coatings, in which the proteins are placed at our command by using 3D printing, is presented. Based on comprehensive spectroscopic and rheological investigations, the optimization of the matrix and the plotting to prepare different micropatterns, i.e., lines, open‐grids, and closed‐grids, is rationalized. The latter are applied to prepare Bio‐WHLEDs with ≈5‐fold enhancement of the luminous efficiency compared to the reference devices with a cascade‐like coating, without losing stability and color quality. As such, this work shows a new route to exploit proteins for optoelectronics, setting a new avenue of research into the emerging field of Bio‐WHLEDs. 相似文献
7.
Yuhan Wang Xinxin Li Debin Zheng Yumiao Chen Zhenghao Zhang Zhimou Yang 《Advanced functional materials》2021,31(45):2102505
Here, a novel strategy to selectively degrade membrane proteins of programmed cell death-ligand 1 (PD-L1) in cancer cells is reported. 4T1 cells show high levels of extracellular alkaline phosphatase (ALP) and membrane proteins of PD-L1. Therefore, peptide derivatives capable of responding to ALP and binding to PD-L1 are designed. Enzyme-instructed self-assembly (EISA) by ALP and surface-induced self-assembly by PD-L1 of Comp. 3 leads to the selective formation of nanomaterials around PD-L1 on the cell membrane, which is similar to attaching a hydrophobic label to the surface of PD-L1 to simulate its partial denaturation state. When taken up by cells, PD-L1 could be further degraded by the proteasome pathway in the cytoplasm. This process does not occur in the normal cell line, LO2 cells, which express relatively low levels of ALP. In addition to concentration and time dependence, the selective knock-out of PD-L1 by the strategy is reversible by simply removing Comp. 3 . In vivo studies reveal that Comp. 3 inhibits tumor growth in a tumor-bearing mouse model. The study offers a novel platform for the precise degradation of membrane proteins using EISA, which might ultimately lead to the development of novel nanomedicines to treat diseases. 相似文献
8.
Aurlie Guyomard Emmanuelle D Thierry Jouenne Jean‐Jacques Malandain Guy Muller Karine Glinel 《Advanced functional materials》2008,18(5):758-765
A non‐water‐soluble natural antibacterial peptide, gramicidin A, has been successfully incorporated into polyelectrolyte assemblies to elaborate biocidal thin films. For this, we used a double strategy, the first step of which consists of complexing the peptide by a non‐denaturing anionic amphiphilic polysaccharide, namely a hydrophobically modified carboxymethylpullulan. We demonstrate that the use of this amphiphilic anionic derivative allows to efficiently solubilize the peptide in aqueous solution, without denaturation. The amount of peptide solubilized by the amphiphilic polysaccharide was optimized by systematically varying the hydrophobicity and the molar mass of the CMP derivative. In a second step, the negatively charged complex was layer‐by‐layer assembled with cationic poly(L‐lysine) to form biofunctionalized thin films. The amount of peptide incorporated in the multilayers was controlled by changing the number of deposited complex layers, and was quantified by UV spectroscopy. The antibacterial activity of the resulting biofunctionalized films was evidenced against a gram‐positive bacterium, E. faecalis. We demonstrated that the biocidal activity resulted from a double mechanism: contact between bacteria and the film surface, and release of the peptide into the solution surrounding the film. We also showed that the peptide was not completely removed from the film after rinsing, which insured preservation of the biocidal activity of the film surface. 相似文献
9.
Wenfei Chen Zhaofei Guo Yining Zhu Nan Qiao Zhirong Zhang Xun Sun 《Advanced functional materials》2020,30(1)
Photothermal therapy (PTT) is a promising cancer treatment, but it has so far proven successful only with relatively small subcutaneous tumors in animal models. Treating larger tumors (≈200 mm3) is challenging because most PTT materials do not efficiently reach the hypoxic, avascular center of tumors, and the immunosuppressive tumor microenvironment prevents T cells from fighting against residual tumor cells, thereby allowing recurrence and metastasis. Here, the widely used PTT material polydopamine is coated on the surface of the facultative anaerobe Salmonella VNP20009, which can penetrate deep into larger tumors. The coated bacteria are intravenously injected followed by near‐infrared laser irradiation at the tumor site, combined with a local inoculation of phospholipid‐based phase separation gel containing the anti‐programmed cell death‐1 peptide AUNP‐12. The gel releases AUNP‐12 sustainably during 42 days, maintaining the tumor microenvironment as immunopermissive. Using a mouse model of melanoma, this triple combination of biotherapy, PTT, and sustainable programmed cell death‐1 (PD‐1) blockade shows high efficiency on eliciting robust antitumor immune responses and eliminating relatively large tumors in 50% of animals within 80 days. Thus, the results shed new light on a previously unrecognized immunological facet of bacteria‐mediated therapy, and this innovative triple therapy may be a powerful cancer immunotherapy tool. 相似文献
10.
Christopher M. Madl Lily M. Katz Sarah C. Heilshorn 《Advanced functional materials》2016,26(21):3612-3620
Covalently‐crosslinked hydrogels are commonly used as 3D matrices for cell culture and transplantation. However, the crosslinking chemistries used to prepare these gels generally cross‐react with functional groups present on the cell surface, potentially leading to cytotoxicity and other undesired effects. Bio‐orthogonal chemistries have been developed that do not react with biologically relevant functional groups, thereby preventing these undesirable side reactions. However, previously developed biomaterials using these chemistries still possess less than ideal properties for cell encapsulation, such as slow gelation kinetics and limited tuning of matrix mechanics and biochemistry. Here, engineered elastin‐like proteins (ELPs) are developed that crosslink via strain‐promoted azide‐alkyne cycloaddition (SPAAC) or Staudinger ligation. The SPAAC‐crosslinked materials form gels within seconds and complete gelation within minutes. These hydrogels support the encapsulation and phenotypic maintenance of human mesenchymal stem cells, human umbilical vein endothelial cells, and murine neural progenitor cells. SPAAC‐ELP gels exhibit independent tuning of stiffness and cell adhesion, with significantly improved cell viability and spreading observed in materials containing a fibronectin‐derived arginine‐glycine‐aspartic acid (RGD) domain. The crosslinking chemistry used permits further material functionalization, even in the presence of cells and serum. These hydrogels are anticipated to be useful in a wide range of applications, including therapeutic cell delivery and bioprinting. 相似文献
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12.
Naroa Serna María Virtudes Céspedes Laura Sánchez‐García Ugutz Unzueta Rita Sala Alejandro Sánchez‐Chardi Francisco Cortés Neus Ferrer‐Miralles Ramón Mangues Esther Vázquez Antonio Villaverde 《Advanced functional materials》2017,27(32)
Protein materials are gaining interest in nanomedicine because of the unique combination of regulatable function and structure. A main application of protein nanoparticles is as vehicles for cell‐targeted drug delivery in the form of nanoconjugates, in which a conventional or innovative drug is associated to a carrier protein. Here, a new nanomedical approach based on self‐assembling protein nanoparticles is developed in which a chemically homogeneous protein material acts, simultaneously, as vehicle and drug. For that, three proapoptotic peptidic factors are engineered to self‐assemble as protein‐only, fully stable nanoparticles that escape renal clearance, for the multivalent display of a CXCR4 ligand and the intracellular delivery into CXCR4+ colorectal cancer models. These materials, produced and purified in a single step from bacterial cells, show an excellent biodistribution upon systemic administration and local antitumoral effects. The design and generation of intrinsically therapeutic protein‐based materials offer unexpected opportunities in targeted drug delivery based on fully biocompatible, tailor‐made constructs. 相似文献
13.
Michael D. Bartlett Andrew B. Croll Alfred J. Crosby 《Advanced functional materials》2012,22(23):4985-4992
The gecko has inspired numerous synthetic adhesive structures, yet under shear loading conditions, general design criteria remains underdeveloped. To provide guidance for bio‐inspired adhesives under shear, a simple scaling theory is used to investigate the relevant geometric and material parameters. The total compliance of an elastic attachment feature is described over many orders of magnitude in aspect ratio through a single continuous function using the superposition of multiple deformation modes such as bending, shear deformation, and tensile elongation. This allows for force capacity predictions of common geometric control parameters such as thickness, aspect ratio, and contact area. This superposition principal is extended to develop criteria for patterned interfaces under shear loading. Importantly, the adhesive patterns under shear are controlled through the compliance in the direction of loading. These predictions are confirmed experimentally using macroscopic building blocks over an extensive range of aspect ratio and contact area. Over 25 simple and complex patterns with various contact geometries are examined, and the effect of geometry and material properties on the shear adhesion behavior is discussed. Furthermore, all of these various attachment features are described with a single scaling parameter, offering control over orders of magnitude in adhesive force capacity for a variety of applications. 相似文献
14.
Jie Luo Miriam Hhn Sren Reinhard Dominik M. Loy Philipp Michael Klein Ernst Wagner 《Advanced functional materials》2019,29(25)
Targeted delivery remains the major limitation in the development of small interfering RNA (siRNA) therapeutics. The successful siRNA multistep delivery requires precise carriers of substantial complexity. To achieve this, a monodisperse carrier is presented, synthesized by solid‐phase supported chemistry. The sequence‐defined assembly contains two oleic acids attached to a cationizable oligoaminoamide backbone in T‐shape configuration, and a terminal azide functionality for coupling to the atherosclerotic plaque‐specific peptide‐1 (AP‐1) as the cell targeting ligand for interleukin‐4 receptor (IL‐4R) which is overexpressed in a variety of solid cancers. For combined cytosolic delivery with siRNA, different apoptotic peptides (KLK, BAK, and BAD) are covalently conjugated via bioreversible disulfide linkage to the 5′‐end of the siRNA sense strand. siRNA‐KLK conjugates provide the highest antitumoral potency. The optimized targeted carrier is complexed with dual antitumoral siEG5‐KLK conjugates. The functionality of each subdomain is individually confirmed. The lipo‐oligomer confers stable assembly of siRNA conjugates into spherical 150–250 nm sized nanoparticles. Click‐shielding with dibenzocyclootyne‐PEG‐AP‐1 (DBCO‐PEG‐AP‐1) mediates an IL‐4R‐specific cell targeting and gene silencing in tumor cells. Most importantly, formulation of the siEG5‐KLK conjugate displays enhanced apoptotic tumor cell killing due to the combined effect of mitotic arrest by EG5 gene silencing and mitochondrial membrane disruption by KLK. 相似文献
15.
Yue Zhao Zuhao Li Shanliang Song Kerong Yang Hou Liu Zhe Yang Jincheng Wang Bai Yang Quan Lin 《Advanced functional materials》2019,29(31)
Recently, artificial intelligence research has driven the development of stretchable and flexible electronic systems. Conductive hydrogels are a class of soft electronic materials that have emerging applications in wearable and implantable biomedical devices. However, current conductive hydrogels possess fundamental limitations in terms of their antibacterial performance and a mechanical mismatch with human tissues, which severely limits their applications in biological interfaces. Here, inspired by animal skin, a conductive hydrogel is fabricated from a supramolecular assembly of polydopamine decorated silver nanoparticles (PDA@Ag NPs), polyaniline, and polyvinyl alcohol, namely PDA@Ag NPs/CPHs. The resultant hydrogel has many desirable features, such as tunable mechanical and electrochemical properties, eye‐catching processability, good self‐healing ability as well as repeatable adhesiveness. Remarkably, PDA@Ag NPs/CPHs exhibit broad antibacterial activity against Gram‐negative and Gram‐positive bacteria. The potential application of this versatile hydrogel is demonstrated by monitoring large‐scale movements of the human body in real time. In addition, PDA@Ag NPs/CPHs have a significant therapeutic effect on diabetic foot wounds by promoting angiogenesis, accelerating collagen deposition, inhibiting bacterial growth, and controlling wound infection. To the best of the authors' knowledge, this is the first time that conductive hydrogels with antibacterial ability are developed for use as epidermal sensors and diabetic foot wound dressing. 相似文献
16.
Chan Wang Yiran Hu Ying Liu Yizhu Shan Xuecheng Qu Jiangtao Xue Tianyiyi He Sijing Cheng Hong Zhou Weixin Liu Zi Hao Guo Wei Hua Zhuo Liu Zhou Li Chengkuo Lee 《Advanced functional materials》2023,33(38):2303696
The reliable function in vivo of self-powered implantable bioelectric devices (iBEDs) requires biocompatible, seamless, effective interactions with biological tissues. Herein, an implantable tissue-adhesive piezoelectric soft sensor (TPSS), in which the piezoelectric sensor converts biomechanical signals into electrical signals, and the adhesive hydrogel (AH) strengthens this conversion by seamlessly adhering the sensor on the wet and curvilinear surface, is proposed. The optimized AH exhibits strong adhesion to various organic or inorganic surfaces, including six commonly used engineering materials and three biological tissues. As a pressure sensor, TPSS proves good in vitro electrical performance with a high output of 8.3 V, long-term stability of over 6000 cycles, and high energy power density of 186.9 µW m−2. In a large animal experiment, TPSS seamlessly adheres to the right-side internal carotid artery of a Yorkshire pig to monitor blood pressure during a surgical operation. Compared to commercial sensors that work by inserting into tissues, TPSS does not cause any damage and can be peeled off after service. The integration of adhesive hydrogel and self-powered pressure sensors enables biocompatible, seamless, and more efficient interactions between the biological system and iBEDs, which also contributes to next-generation implantable bioelectronics with features of battery-free, intelligent, and accurate. 相似文献
17.
Michael C. Wilson Qin Lu Kaitlin R. Nachtrieb Jackson S. Fuller Chloe M. Skogg Elizabeth A. Yates Matthew D. Thum Christopher R. So 《Advanced functional materials》2024,34(3):2308790
Barnacles convert hydrophilic proteins into an insoluble, yet aqueous, material that functions as a permanent underwater adhesive. Here, it is demonstrated that a common hydrophilic protein, bovine serum albumin, can be chemically triggered underwater to aggregate into a similar aqueous adhesive that mimics the formation of the natural adhesive. The combined action of multiple chemical denaturants initiates rapid gelation followed by further curing over time in artificial seawater. The adhesive strengths of this waterborne adhesive measured by lap shear are comparable to many bioinspired adhesives that use organic solvents and a high fraction of hydrophobic components. This approach establishes a bioinspired adhesive that can be deployed at practical scales in marine environments, produced sustainably, and sourced from low-cost materials. 相似文献
18.
Xiaohua Yu Andrew Khalil Phuong Ngoc Dang Eben Alsberg William L. Murphy 《Advanced functional materials》2014,24(20):3082-3093
There is an increasing need to control the type, quantity, and timing of growth factors released during tissue healing. Sophisticated delivery systems offering the ability to deliver multiple growth factors with independently tunable kinetics are highly desirable. Here, a multilayered, mineral coated microparticle (MCMs) platform that can serve as an adaptable dual growth factor delivery system is developed. Bone morphogenetic protein‐2 (BMP‐2) and vascular endothelial growth factor (VEGF) are bound to the mineral coatings with high binding efficiencies of up to 80%. BMP‐2 is firstly bound onto a 1st mineral coating layer; then VEGF is bound onto a 2nd mineral coating layer. The release of BMP‐2 is sustained over a period of 50 days while the release of VEGF is a typical two‐phase release with rapid release in the first 14 days and more sustained release for the following 36 days. Notably, the release behaviors of both growth factors can be independently tailored by changing the intrinsic properties of the mineral coatings. Furthermore, the release of BMP‐2 can be tuned by changing the thickness of the 2nd layer. This injectable microparticle based delivery platform with tunable growth factor release has immense potential for applications in tissue engineering and regenerative medicine. 相似文献
19.
Wen Li Wei Bing Sa Huang Jinsong Ren Xiaogang Qu 《Advanced functional materials》2015,25(24):3775-3784
Inspired by the load‐bearing biostructures in nature, a multifunctional shell for encapsulating cell using the polyphenol–metal complexes is fabricated. The artificial shell is formed by cross‐linking of tannic acid and iron ion on cell surface. It can protect cells from unfriendly environments, including UV light irradiation and reactive oxygen damage. With the hybrid property of polyphenol and metal liands, the shell provides a versatile platform for cell surface engineering. The magnetic nanoparticles, DNA molecules, as well as the magnetic resonance imaging agents are easily incorporated into the shell. More interestingly, unlike the traditional passive coatings, here the shell can be controllably disassembled under external stimuli. The dynamic coating is used as a reversible element to regulate cell division and surface modification. The cell viability and protein expression experiments further confirm that the shell formation and degradation processes are biocompatible. This multifunctional coating strategy is applicable to multiple living cell types, including yeast cells, Escherichia coli bacteria, and mammalian cells. Therefore, this platform would be useful for living cell based fundamental research and biological applications. 相似文献