Salmonella contamination of laying-hen flocks in two regions of Algeria

A preliminary epidemiological study of Salmonella contamination in laying-hen flocks was carried out in the regions of Annaba and Eltarf, Algeria, from March to October 2008 and March to November 2009. Our objectives were (i) to estimate the prevalence of infection by Salmonella spp. in seven pooled samples during the hens' laying period (ii) to identify the serotypes and antimicrobial resistance phenotypes of isolates, and (iii) to characterize the factors that may be related to Salmonella contamination in Algerian henhouses. For this purpose, 18 out of 22 operational laying-hen houses were sampled one to three times during these periods: once at the start of laying (pullets aged 22–31 weeks), once in the middle of laying (47–60 week) and once at the end of laying prior to depopulation (70–86 week). The flocks'Salmonella status was assessed by collecting 2754 environmental samples that were analyzed according to the ISO 6579 method. The antibiotic resistance of Salmonella strains was tested as per the guidelines of the Clinical and Laboratory Standards Institute (CLSI). The relationship between each potential risk factor and the Salmonella status of laying-hen flocks was evaluated by calculating the relative risk with 95% confidence intervals. Eight flocks tested positive for Salmonella spp., with a higher prevalence at the end of laying than at either the beginning or middle. Only 19 isolates were recovered from the 2754 samples analyzed and nine different serotypes identified. S. enteritidis (n = 4) was the most prevalent serovar, along with S. Kentucky and S. Hadar (n = 3), followed by S. Heidelberg, S. Manhattan and S. Virchow (n = 2), whereas S. Dublin, S. Typhimurium and S. Albany were found only once. Thirteen isolates were resistant to at least one antimicrobial agent. Of these, six were resistant to at least three different antimicrobial classes. Salmonella serovar Kentucky isolates were resistant to fluoroquinolones with ciprofloxacin MIC ≥ 8 mg/L. Six risk indicators were identified as potentially related to the Salmonella status of layer houses.     

Variation of bacteriologic and serologic Salmonella enterica prevalence between cohorts within finishing swine production farms

The objective of this study was to evaluate the consistency of bacteriologic and serologic Salmonella enterica prevalence in cohorts of finishing pig lots from multiple production farms. A total of 6 lots of finishing pigs from each of 6 finishing production farms were included in this study. For each lot studied, 30 individual fecal samples were collected directly from the rectum immediately before the pigs were transported to the abattoir, and 50 individual meat samples were collected at slaughter. Individual fecal and meat juice samples were processed for detection of Salmonella and antibodies against Salmonella, respectively. All finishing production farms were Salmonella-positive in at least 2 fecal and 4 meat samplings. The overall bacteriologic prevalence was 12.9% (95% C.I. 8.0–17.8%), whereas the serologic prevalence was 35.4% (95% C.I. 24.5–46.4%; P < 0.05). A wide variation in Salmonella prevalence (bacteriologic and serologic) between different finishing pig lots within production farms was observed, preventing the categorization of the production farms as either high or low Salmonella prevalence. This study shows that bacteriologic and serologic estimates of Salmonella prevalence are not consistent among cohorts within the same production farm, suggesting that point estimates of Salmonella prevalence in swine populations are not reliable.     

A systematic review and meta-analysis of the effectiveness of biosecurity and vaccination in reducing Salmonella spp. in broiler chickens

Our objective was to estimate the effectiveness of vaccination and biosecurity on the prevalence of Salmonella spp. in broiler chickens using systematic review and meta-analysis. A comprehensive search of the global primary literature was conducted in: Current Contents (1999–2009), Agricola (1924–2009), MEDLINE (1860–2009), Scopus (1960–2009), CAB (1913–2009), and Centre for Agricultural Bioscience Global Health (1971–2009). The search algorithm was (Salmonell*) AND (chicken* OR chick* OR poultry* OR broiler* OR gallus*). Additional studies were identified by contacting five topic experts and hand-scanning bibliographies of recent review articles and a recently published textbook. Studies were included if they were English language and investigated the effects of vaccination and biosecurity on the prevalence of Salmonella spp. in broiler chickens. All study design types were included. Data extraction and methodological assessment were conducted by two reviewers independently. All meta-analyses were based on random-effects models. For biosecurity, sixteen challenge studies (n = 137 treatment-control comparisons) and one controlled study (n = 2) met the inclusion criteria. Significant heterogeneity (Cochran's Q-statistic, p < 0.001) was observed among biosecurity challenge studies examining hydrogen peroxide or polyhexamethylenebiguanide hydrochloride applied to hatching eggs, making it inappropriate to present a summary effects measure. For vaccination, 19 challenge studies (n = 226) and three controlled studies (n = 10) met the inclusion criteria. Among live Salmonella Typhimurium vaccine challenge studies heterogeneity was not significant (p = 0.138). Vaccination with a live Salmonella Typhimurium reduced the risk of Salmonella cecal colonization in the treated broiler group by 35 out of 1000 broilers when compared to the control group (OR = 0.21; 95% CI = 0.06–0.77) and this effect was significant (p = 0.018). One biosecurity study (n = 2 treatment-control comparisons) and three vaccination studies (n = 10) were conducted in a commercial setting. The two included studies in the vaccination meta-analysis were both conducted at research facilities. The live Salmonella Typhimurium vaccine showed the most promise in reducing the prevalence of Salmonella in broiler ceca. However, the meta-analysis included few studies, and these studies challenged the birds with different serotypes. We recommend that more large-scale randomized, blinded trials be conducted with a live Salmonella Typhimurium vaccine on commercial farms.     

Salmonella in meat from hunted game: A Central European perspective

The occurrence of Salmonella in meat from game hunted in Europe is reviewed, with a focus on Central Europe. Prevalence in fecal samples is different according to region and game species, ranging from < 5 (wild ruminants) to ca. 20% (wild boar). The pathogen is rarely recovered from carcasses or meat cuts of wild ruminants (< 1%). Prevalences on wild boar carcasses have been reported to range from < 1 to ca. 7%, the tonsils presumably constituting a main reservoir on the eviscerated carcass. On meat cuts from game, recent German and EU (EFSA) reports indicate a prevalence of < 1%. A number of Salmonella enterica serovars can be isolated, with highest prevalences of S. Typhimurium and S. Enteritidis. Persistence of these pathogens in wildlife is a less pressing issue for biology and wildlife conservation than for public health experts, which has been recognized in the framework of zoonoses legislation. On one hand, wildlife is exposed to salmonellae originating from farm animals and humans, and on the other hand, game can be a source of Salmonella in farm animals. Breeding game under intensified conditions (farmed game) as well as extensive breeding of farm animals, notably pigs, can create new epidemiological situations. The pathway of Salmonella from intestinal content of the live animal to individual meat cuts can be described in qualitative terms, yet the relative contribution of the various factors remains to be quantified, which complicates proper risk assessment. Control of Salmonella in the game meat chain is in part based on Good Hygiene Practice from killing and evisceration to chilling and skinning, whereas more detailed data are needed in order to allow risk-based meat inspection.     

Biofilm formation,virulence gene and multi-drug resistance in Salmonella Kentucky isolated in Tunisia

Food-borne diseases caused by Salmonella enterica are a significant public health concern around the world. Since 2002, S. enterica serovar Kentucky has shown an increase in several countries with the concurrent emergence of multidrug-resistant isolates. The spread of such strains in the environment poses a major public health problem. A total of 57 Salmonella Kentucky strains isolated from different sources during the period 2005 to 2008 in Tunisia, were characterized by their antimicrobial and mercury resistance profiles; ability to form a biofilm; virulence invA/spvC genes and quorum sensing sdiA gene. A total of 10.6% of the isolates demonstrated multidrug-resistance against 3 to 13 antibiotics with ciprofloxacin resistance occurring in 33% of human isolates. In addition, 37% of the isolates exhibited minimum inhibitory concentrations value to mercuric chloride, ranging from 8 to 32 μg ml^?1 and were considered as resistant strains. The majority of strains tested were able to form a biofilm, especially for environmental and animal derived isolates. Therefore, the biofilm seems to comprise a normal and favorable capability in the life of Salmonella Kentucky in the environment. Interestingly, all the isolates possessed the sdiA gene, 87.7% of isolates possessed the invA gene, and no isolate harbored the spvC gene.The emergence of resistance to ciprofloxacin in human Salmonella Kentucky isolates, added to the presence of invA and sdiA genes, and the production of biofilm could be the decisive factors in the dissemination of S. Kentucky strains on a large scale.     

Radio-sensitivity of pathogens in inoculated prepared foods of animal origin

The effectiveness of irradiation for inactivating Staphylococcus aureus, Listeria ivanovii, Salmonella Typhimurium, and Escherichia coli in the prepared foods of animal origin was investigated. Commercially available seasoned and cooked beef, fried egg, and ham were purchased, radiation-sterilized, and inoculated at 10⁶–10⁷ CFU/g with each of the four pathogens and stored at three storage conditions at 10 °C, 20 °C, and 30 °C. D₁₀-values of S. aureus, L. ivanovii, Salmonella Typhimurium, and E. coli were 0.34±0.01, 0.24±0.02, 0.24±0.01, and 0.27±0.01 kGy, respectively. No viable cells were detected at 3 kGy of irradiation. Salmonella mutagenicity assay (Ames test) indicated that the 10 kGy irradiated samples were statistically similar to non-irradiated control samples in the Salmonella mutagenicity assay (Ames test). These studies demonstrate that irradiation can be used as an additional safety tool to produce microbiologically safe and wholesome prepared foods of animal origin.     

Inactivation of Salmonella Typhimurium and Staphylococcus aureus by pulsed electric fields in liquid whole egg

In this study, the lethal effectiveness of pulsed electric fields (PEF) on the inactivation of Salmonella enterica subs. enterica ser. Typhimurium and Staphylococcus aureus in liquid whole egg (LWE) has been investigated. Maximum inactivation levels of 4 and 3 Log₁₀ cycles of the population of Salmonella Typhimurium and S. aureus were achieved with treatments of 45 kV/cm, 30 μs and 419 kJ/kg, and 40 kV/cm for 15 μs and 166 kJ/kg, respectively. The non-linear kinetics of inactivation observed for both microorganisms at all the investigated electric field strengths were described by mathematical equations based on the Weibull distribution. The developed equations enabled to compare the microbial resistance to PEF and to establish the most suitable treatment conditions to achieve a determined level of microbial inactivation. PEF treatments varying from 30 kV/cm, 67 µs and 393 kJ/kg to 45 kV/cm, 19 µs and 285 kJ/kg allow to reduce 3 Log₁₀ cycles the population of the microorganism of concern in PEF food processing of LWE, Salmonella Typhimurium.Industrial relevanceThe data presented in this investigation in terms of electric field strength, specific energy and treatment time result of relevance to evaluate the possibilities of PEF technology to pasteurize LWE with this technology. The models developed in this study can be applied to engineering design, and for the evaluation and optimization of the PEF technology as a new technique to obtain Salmonella free LWE.Based on our results it is not recommended to apply treatments of energy levels higher than 250 kJ/kg, since PEF lethality hardly increased but markedly augmented the energetic costs. For these energy values, PEF technology by itself is not sufficient (3 Log10 cycles in the best case scenario) to assure the safe security of LWE. Therefore, intelligent combinations of PEF with other preservation technologies have to be developed in order to use pulsed electric fields as an alternative to heat pasteurization of LWE.     

Growth of Salmonella serovars in hens’ egg albumen as affected by storage prior to inoculation

Different Salmonella enterica serovars, including Enteritidis, were tested for growth at 20°C in separated albumen upon inoculation with 39 cfu ml⁻¹. The albumen was fresh or stored for up to 3 weeks prior to inoculation (p.i.) either in the shell egg or separated from the yolk. The serovar Enteritidis did not behave differently than the other serovars indicating that the association between human S. Enteritidis infections and eggs is not due to its growth behaviour in albumen. A pronounced growth occurred more frequently and up to a one-log unit higher level in fresh albumen than in albumen stored p.i. This was at least partly explained by a pH effect. Since growth in the separated albumen was similar when the albumen had been stored p.i. in the absence or presence of yolk, we have no indication that nutrients or factors negating the inhibitory properties of the albumen leak out from the yolk during storage. Growth of Salmonella inoculated at a level of 8 cfu in the albumen of fresh and stored whole shell eggs was studied to simulate a more natural situation. In this case, growth also occurred more frequently when inoculated in the albumen of fresh eggs compared to eggs stored p.i. It can be concluded from our study that cooling practices are recommended shortly after lay to prevent Salmonella from growing in eggs.     

Application of bacteriophages during depuration reduces the load of Salmonella Typhimurium in cockles

As bivalve molluscs are filter feeder, often consumed raw or lightly cooked and are frequently cultivated in contaminated waters, they are implicated in food-borne disease transmission to human. The present study investigated the potential application of bacteriophage (or phage) phSE-2, phage phSE-5 and phage cocktail phSE-2/phSE-5 to decrease the concentration of Salmonella enterica serovar Typhimurium (Salmonella Typhimurium) during the depuration of natural and artificially contaminated cockles (Cerastoderma edule). Cockles were artificially infected with 10⁵ and 10⁶ colony-forming units (CFU)/mL of S. Typhimurium in static seawater and infected group were treated with phages at four different MOI values: 0.1, 1, 10 and 100. Depuration in static seawater at multiplicity of infection (MOI) of 0.1 with single phage suspensions of phSE-2 and phSE-5 provided the best results, as it decreased by ~ 1.3 and 1.7 log CFU/g, respectively, the concentration of Salmonella spp. after a 4 h treatment. At a MOI of 0.1, the rate of inactivation with single phage suspensions was higher when compared with the results obtained using the phage cocktail. However, in naturally contaminated cockles treated in static seawater with single phage suspensions and phage cocktail phSE-2/phSE-5, similar decreases in cultivable bacteria concentration (~ 0.7–0.9 log CFU/g) were achieved after 6 h of treatment. When artificially contaminated cockles were depurated with phage phSE-5 in a recirculated seawater system (mimicking industrial depuration conditions), a 0.9 and 2.0 log CFU/g reduction of Salmonella spp. was reached after 4 and 6 h treatment. Once the depuration process was performed without phage, a 6 h treatment was needed to obtain a 1.1 log CFU/g reduction of Salmonella spp. Results indicated that combining phage biocontrol with depuration procedures enhance bivalve microbial safety for human consumption by improving decontamination efficiency, proving that this technology can be transposed to the bivalves industry. Moreover, this approach also displays the advantage of reducing the time required for depuration and consequently its associated costs.     

Inactivation kinetics of Listeria monocytogenes,Salmonella enterica serovar Typhimurium,and Campylobacter jejuni in ready-to-eat sliced ham using UV-C irradiation

To investigate the applicability of UV-C irradiation on the inactivation of foodborne pathogenic bacteria in ready-to-eat sliced ham, UV-C treatment was evaluated. Irradiation dose required for 90% reduction of the populations of Listeria monocytogenes, Salmonella enterica serovar Typhimurium, and Campylobacter jejuni were determined to be 2.48, 2.39, and 2.18 J/m². Ready-to-eat sliced hams were inoculated with the pathogens and irradiated with UV-C light of 1000, 2000, 4000, 6000, and 8000 J/m². Microbiological data indicated that foodborne pathogen populations significantly (p < 0.05) decreased with increasing UV-C irradiation. In particular, UV-C irradiation at 8000 J/m² reduced the populations of L. monocytogenes, S. Typhimurium, and C. jejuni in the ham by 2.74, 2.02, and 1.72 log CFU/g. The results indicate that UV-C irradiation can be used as a microbial inactivation method for ready-to-eat sliced ham, and inactivation kinetics of the foodborne pathogens fit the Weibull model better than the first-order kinetics model.     

Response surface methodology to investigate the effect of high pressure processing on Salmonella inactivation on dry-cured ham

The lethal effect of high pressure on microorganisms is influenced by a number of factors in relation to the technological variables, microorganisms and food matrix, which have to be considered when designing high pressure processes. The present work aimed to develop and validate a model of the inactivation of Salmonella enterica on dry-cured ham by high pressure processing (HPP), as a function of the technological parameters: intensity, length and fluid temperature. Dry-cured ham inoculated with S. enterica was treated at different HPP conditions (at 347 to 852 MPa; for 2.3 to 15.75 min; at 7.6 to 24.4 °C) following a central composite design. Bacterial inactivation was assessed in terms of logarithmic reductions of Salmonella counts on selective media. According to the best fitting and most significant polynomial equation, pressure and time were the most important factors determining the inactivation extent. Temperature showed significant influence through its interaction with both pressure and holding time. The model was validated with results obtained from further experiments within the range of the experimental domain. The accuracy factor and bias factor were within the proposed acceptable values indicating the suitability of the model for predictive purposes, for instance to predict the process criteria to meet the lethality safety standards. The results of this work may help food processors to select optimum processing conditions of HPP to ensure the microbiological safety of pressure-treated foods.     

Fecal prevalence and diversity of Salmonella species in lactating dairy cattle in four states

Salmonella is one of the most serious foodborne pathogenic bacteria in the United States, causing an estimated 1.3 million human illnesses each year. Dairy cows can be reservoirs of foodborne pathogenic bacteria, including Salmonella spp.; it is estimated that from 27 to 31% of dairy herds across the United States are colonized by Salmonella. The present study was designed to examine the occurrence of Salmonella spp. on dairies and to examine the serotypic diversity of Salmonella isolates on sampled dairies from across the United States. Fecal samples (n = 60 per dairy) were collected from 4 dairies in each of 4 states for a total of 960 fecal samples representing a total population of 13,200 dairy cattle. In the present study, 93 of 960 samples (9.96%) collected were culture-positive for Salmonella enterica. At least one Salmonella fecal-shedding cow was found in 9 of the 16 herds (56%) and the within-herd prevalence varied in our study from 0% in 7 herds to a maximum of 37% in 2 herds, with a mean prevalence among Salmonella-positive herds of 17%. Seventeen different serotypes were isolated, representing 7 different Salmonella serogroups. There were 2 or more different serogroups and serotypes present on 7 of the 9 Salmonella-positive farms. Serotypes Montevideo and Muenster were the most frequent and widespread. From our data, it appears that subclinical colonization with Salmonella enterica is relatively common on dairy farms and is represented by diverse serotypes on US dairy farms.     

Survival of Salmonella Enteritidis PT 30 on inoculated almond kernels in hot water treatments

Almonds are blanched by exposure to hot water or steam-injected water to remove the pellicle (skin) from the kernel. This study evaluated the survival of Salmonella Enteritidis PT 30, Salmonella Senftenberg 775W and Enterococcus faecalis on whole raw almond kernels exposed to hot water. Whole, inoculated (7 to 9 log CFU/g) Nonpareil almonds (40 g) were submerged in 25 L of water maintained at 60, 70, 80 and 88 °C. Almonds were heated for up to 12 min, drained for 2 s, and transferred to 80 mL of cold (4 °C) tryptic soy broth. Almonds in broth were stomached at high speed for 2 min, serially diluted, plated onto tryptic soy and bismuth sulfite agars (Salmonella) or bile esculin agar (Enterococcus) and incubated at 37 °C for 24 and 48 h, respectively. D values of 2.6, 1.2, 0.75 and 0.39 min were calculated for exposure of S. Enteritidis PT 30 to water at 60, 70, 80 and 88 °C, respectively; the calculated z value was 35 C°. D values determined for Salmonella Senftenberg 775W and E. faecalis at 88 °C were 0.37 and 0.36 min, respectively. Neither Salmonella serovar could be recovered by enrichment of 1-g samples after almonds inoculated at 5 log CFU/g were heated at 88 °C for 2 min. These data will be useful to validate almond industry blanching processes.     

A meta-analytical estimation of the detection limits of methods for Salmonella in food

The validation of microbial detection methods for foods does not typically state a limit of detection value. Therefore performances of rapid and reference methods for Salmonella detection in foods were compared retrospectively using data from 49 published studies. A total of 576 values for the limit of detection (LOD₅₀) were calculated. The major scientific variables in these independent validation studies were food matrices, Salmonella serovars, and method types (reference, cultural and immunological and nucleic acid based). The basic design feature of the original studies was comparison of the performances of new methods with those of cultural reference methods. A major experimental design variable was the number of laboratories per study. There were 29 single laboratory studies with 20 replicates per level of Salmonella spiked into the food matrix. The 20 multi-laboratory (N ≥ 10) studies had 5–6 replicates per level of Salmonella. The LOD₅₀ values of the dataset had a mean value of 0.021 MPN g^? 1 (standard deviation range (0.013–0.036) and the distribution of their logarithms was symmetrical about the logarithm of the mean if the outlier values were discounted. Eighty-nine percent of the values ranged from 0.01 to 0.04 MPN g^? 1. On this basis about 11% of the values were deemed outliers. About three-quarters of them were > 0.04 MPN g^? 1. The distribution derived in this study can be used as a bench mark against which to evaluate LOD₅₀ data generated in future studies of detection methods for Salmonella in foods and potentially offers a possible way to streamline method validation study experimental design.     

Development of a fimY-based loop-mediated isothermal amplification assay for detection of Salmonella in food

In this study, we developed a rapid and sensitive fimY-based loop-mediated isothermal amplification (LAMP) assay on a real-time turbidimeter platform for the detection of Salmonella in food. Since turbidity of the reaction mixture would increase in correlation with the DNA yield, real-time monitoring of the LAMP reaction was achieved by real-time turbidimeter. Time threshold values which indicate positive results for 81 Salmonella strains of different serotypes ranged from 36 to 40 min. For the 20 non-Salmonella strains, turbidity did not increase in the reaction mixture. When testing 10-fold serial dilutions of Salmonella Typhimurium-ATCC 14128 DNA by LAMP, the time threshold ranged from 36 to 52 min on the real-time turbidimeter. The detection limit was 13 cells per reaction in pure culture, up to 10-fold more sensitive than that of PCR. When applied in deli food samples, the LAMP assay was able to detect Salmonella even though the sample was contaminated with very low concentration after 3 h enrichment culture. Increase in turbidity was observed on real-time turbidimeter. Additionally, the LAMP results detected by naked-eye or turbidity consistently matched with each other. Results from this study showed that the fimY-based LAMP assay is an effective method for the rapid detection of Salmonella.     

Antimicrobial resistance of Salmonella isolated from food animals: A review

Salmonella enterica is recognized as one of the most common causes of bacterial foodborne illness worldwide. The majority of Salmonella infections are attributed to consumption of contaminated food of animal origin such as eggs, chicken, pork, etc. Severe Salmonella infections often require antimicrobial therapy to aid in the elimination of the infection. A potential problem that has been developing for many decades is the development of antimicrobial resistance. There has been an increasing concern over the past 30 years regarding the worldwide emergence of multidrug-resistant phenotypes among Salmonella serotypes such as S. Typhimurium, S. Enteritidis and S. Newport. A special concern is the emergence of resistance to quinolones, fluoroquinolones or extended-spectrum cephalosporins such as ceftiofur and ceftriaxone. Recently, the occurrence of Salmonella isolates resistant to these antibiotics has increased. Therefore, continuous monitoring of its prevalence and resistance in the food supply is necessary because of the public health implications of a potential spread of resistant microorganisms. Furthermore, a holistic animal management approach such as stringent control of antimicrobial agents in the livestock industry, early clinical and microbiological diagnosis, appropriate treatment, and implementation of strict sanitary standards in the food industry are also needed to significantly reduce the overall burden of salmonellosis on human health.     

Integrated surveillance of Salmonella along the food chain using existing data and resources in British Columbia,Canada

Integrated surveillance of pathogens along the food chain and the multidisciplinary investigation of food hazards are considered international best practices. Integrated surveillance of Salmonella was initiated in British Columbia (BC), Canada in 2006. The objectives of this paper were 1) to describe the BC integrated surveillance experience, 2) to present findings from the integrated surveillance of Salmonella, and 3) to identify the components that enabled the program. Data about BC animal, food and human Salmonella isolates from 2006 to 2010 (n = 5003) were centrally collated and analysed. Among chickens, chicken meat and humans, the most common serotypes identified were S. Enteritidis, S. Typhimurium and S. Heidelberg. An increase in S. Enteritidis in all three sectors in 2007–9 led to a multi-disciplinary and multi-sectoral investigation. An evaluation of the integrated surveillance program helped identify four critical program elements: dedicated people, cross-sectoral sharing and integration of data, multi-disciplinary analysis and interpretation of findings, and collaborative multi-sectoral response. Ongoing challenges include lack of resources and infrastructure to sustain the program.     

High pressure treatments on the inactivation of Salmonella Enteritidis and the characteristics of beef carpaccio

The effect of high pressure (HP) on Salmonella enterica subsp. enterica serovar Enteritidis in beef carpaccio stored under temperature abuse conditions (8 °C) during 30 days was investigated. After treatment, reductions of S. Enteritidis were 3.68 and 5.94 log cfu/g in samples pressurized at 450 MPa for 5 and 10 min, respectively, whereas the pathogen was only detected after enrichment of samples treated at 450 MPa for 15 min. During storage, counts of S. Enteritidis decreased 0.26 log cfu/g in non-pressurized carpaccio, 1.33 log cfu/g in carpaccio treated at 450 MPa for 5 min and were only detected after enrichment in carpaccio pressurized at 450 MPa for 10 or 15 min. Color (L*, a* and b*) varied with pressurization and storage, with higher changes in carpaccio treated at longer pressurization times. Shear resistance was slightly lower in treated samples just after pressurization, but increased at the end of the storage period. Maximum force was less affected by treatment.     

Antibiotic resistance and susceptibility to some food preservative measures of spoilage and pathogenic micro-organisms from spices

Antibiogram of 84 strains of Bacillus cereus, 26 strains of Clostridium perfringens, four strains of Staphylococcus aureus, 51 strains of Enterobacteriaceae, two strains of each of Salmonella and Shigella; isolated from spices, were studied against 20 different antibiotics that are commonly used against foodborne diseases, mainly gastroenteritis. All the tested strains of B. cereus, Cl. perfringens, Staph. aureus, Escherichia coli, Salmonella and Shigella were found resistant to at least 3, 4, 7, 6, 10 and 9 antibiotics, respectively. In brain–heart infusion broth supplemented with glucose, the D_100°C-values for B. cereus were 3.5–5.9 min, and the z-values were 17–18°C. The D_100°C-values for Cl. perfringens in fluid thioglycolate medium were higher (10.0–19.8 min) than those of B. cereus. The minimum inhibitory concentrations (MICs) of sodium chloride were 45–80 mg ml⁻¹. While the MIC of benzoic acid for Cl. perfringens, tested on perfringens agar (pH 7.3) plates by incubating anaerobially at 35°C for 24 h, was 1.9–2.2 mg ml⁻¹, for others, tested on nutrient agar (pH 6.8) plates by incubating at 35°C for 18 h in static aerobic condition, it was much less. Similarly, the MIC of sorbic acid for all the tested isolates, excepting Cl. perfringens, was 0.6–1.1 mg ml⁻¹. Of the eight isolates of Cl. perfringens, only three were inhibited at 2.0 mg sorbic acid ml⁻¹, while others were resistant. Sixty percent and 75% of the respective strains of B. cereus and Cl. perfringens were resistant to 5000 IU Nisaplin ml⁻¹, whereas the MIC values of Staph. aureus were between 3000 and 5000 IU ml⁻¹. While studying combined effect of selected hurdles on the growth of enterotoxigenic Cl. perfringens 16-C2, the judicious combination considered was low acid (pH 6.0), 30 mg sodium chloride ml⁻¹ and 1.25 mg benzoic acid ml⁻¹.     

Comparison of antimicrobial resistance patterns and phage types of Salmonella Typhimurium isolated from pigs,pork and humans in Belgium between 2001 and 2006

Infections with non-typhoid Salmonella represent a major problem in industrialized countries.The emergence and spread of antimicrobial-resistant pathogens, among them Salmonella, has become a serious health hazard worldwide. One of the most commonly isolated non-typhoid Salmonella serovars in pigs, pork and humans is Salmonella Typhimurium. In this study the comparison of the incidences of resistance to nine antimicrobials, resistance patterns and phage types between S. Typhimurium isolated from pigs (n = 581), pork (n = 255) and humans (n = 1870) in Belgium in the period 2001 to 2006 was performed.Resistance to the antimicrobials ampicillin, chloramphenicol, streptomycin, sulfonamides and tetracycline was frequently observed and varied between 23.5% and 83.1%. Resistance ranged from 15.6% to 20.7% for the combination trimethoprim–sulfonamides and from 3.4% to 5.8% for nalidixic acid. Resistance to the critical important antimicrobials cephalosporins and fluoroquinolones was found sporadically (≤ 1.2%). Resistance to the different antimicrobials was observed to be similar in S. Typhimurium isolates from the various origins. Twenty-seven antimicrobial resistance patterns representing in total 75.2%, 89.0% and 89.6% of the isolates from pigs, pork and humans respectively were found to be common among the three groups and 73 combinations antimicrobial resistance pattern/phage type were found to be common among pork and human isolates, representing 70.1% of the pork isolates and 51.0% of the human isolates. The high percentage of isolates that have a common resistance pattern, and in a less pronounced way a common combination phage type/resistance pattern, are in agreement with the hypothesis of transfer of antimicrobial resistant Salmonella from pigs via the consumption of pork to humans as one of the possible pathways. The most prevalent combination in Belgium within both the pork isolates (7.4%) and the human isolates (13.2%) was S. Typhimurium DT104 resistant to ampicillin, chloramphenicol, streptomycine, sulfonamides and tetracycline.