共查询到18条相似文献,搜索用时 78 毫秒
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综述了基于激发态分子内质子转移(ESIPT)荧光探针的相关研究,并对ESIPT机理和ESIPT荧光团的2-(2'-羟苯基)苯并杂环类(咪唑、(噁)唑、噻唑)及其衍生物等的主要结构进行了系统介绍.重点介绍了双发光机制荧光探针在检测方面的应用进展. 相似文献
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次氯酸是一种来源于线粒体的活性氧,在各种生理和病理过程中起着重要的作用。但是,当细胞中的HOCl浓度超过正常值时范围,它会导致机体损伤和一系列疾病。因此,近年来开发设计了一系列能实时识别和监测线粒体中的次氯酸水平的荧光探针,这有助于更好地了解生物体健康状况和HOCl起到的生理作用和病理过程。主要介绍了近几年HOCl荧光探针的应用和发展,根据靶向线粒的基团类别,分别介绍了三苯基膦类荧光探针,半花菁类荧光探针,氟硼吡咯类荧光探针。 相似文献
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本研究基于分子内质子转移机制(ESIPT),制备了一种具有选择性识别Cys的反应型荧光探针(探针1)。在乙腈-磷酸盐缓冲溶液(3:7,体积比,pH 7.4)中,探针1的荧光强度与Cys的浓度在10~100μmol/L范围内呈现良好的线性关系(y=4.127x+42.84, R2=0.9978),检测限为36.7 nmol/L。该探针对Cys的响应具有较大的Stokes位移(160 nm)、高灵敏度和选择性。此外,探针1还具有良好的细胞膜通透性和生物相容性,可用于A549细胞中Cys的荧光成像,在生物分析中具有潜在的应用价值。 相似文献
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以香豆素为荧光团,4-溴丁酰基为识别基团,设计合成了一种比率型肼荧光探针COCB。其结构通过1HNMR、13CNMR和HRMS确证。肼对探针COCB中溴代丁酰基的选择性脱保护使分子内电荷转移(ICT)过程恢复;COCB在 420 nm 处蓝色荧光衰减,而在 480 nm 处青色荧光增强,实现了对肼的比率检测。COCB对肼表现出高选择性、高灵敏度和强抗干扰能力,并能在较宽的线性范围(0~250 μmol/L)和pH范围(6~11)内检测肼,检出限低至0.15μmol/L。此外,COCB合成简便,细胞毒性较低,已成功用于实际水样、土壤以及活细胞中肼的检测。 相似文献
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Yunjian Cao Xiangrui Yu Chaofan Sun Jingang Cui 《International journal of molecular sciences》2022,23(4)
Recently, a new fluorescent probe AE-Phoswas reported to detect the activity of alkaline phosphatases (ALP) in different living cell lines. Here, we present an in-depth computational analysis of the mechanism and source of the fluorescence of the AE-Phos probe. There is an intermediate product (AE-OH-Phos) in the experiment as well as a different configuration of products that may emit fluorescence. It is essential to investigate the origin of fluorescence and the detection mechanism of the probe, which could help us eliminate the interference of other substances (including an intermediate product and possible isomers) on fluorescence during the experiment. According to the change of geometric parameters and Infrared spectra, we deduce that the dual intramolecular hydrogen bonds of salicylaldehyde azine (SA) were enhanced at the excited state, while AE-OH-Phos was attenuated. Considering the complex ESIPT behavior of the dual proton-type probe, the potential energy surfaces were further discussed. It can be concluded that the single proton transfer structure of SA (SA-SPT) is the most stable form. Both the concerted double proton transfer process and stepwise single proton transfer process of SA were forbidden. The fluorescence for SA was 438 nm, while that of SA-SPT was 521 nm, which agrees with the experimentally measured fluorescence wavelength (536 nm). The conclusion that single proton transfer occurs in SA is once again verified. In addition, the distribution of electron-hole and relative index was analyzed to investigate the intrinsic mechanism for the fluorescence quenching of the probe and the intermediate product. The identification of the origin of fluorescence sheds light on the design and use of dual-proton type fluorescent probes in the future. 相似文献
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水溶性萘酰亚胺氢离子荧光分子探针的合成及性能 总被引:1,自引:0,他引:1
4 溴 1,8 萘酐与羟乙氧基乙胺反应合成了中间体N 羟乙氧基乙基 4 溴 1,8 萘酰亚胺。用该中间体分别与哌嗪、甲基哌嗪和羟乙基哌嗪反应 ,合成了 4 哌嗪基、4 (甲基哌嗪 )基和 4 (羟乙基哌嗪 )基 1,8 萘酰亚胺衍生物 (NP - 1、NP - 2和NP - 3)。这 3种化合物具有较好的水溶性 ,其水溶液的荧光强度随溶液由碱性到酸性变化 ,荧光强度增加在 5 0倍以上。NP - 1、NP - 2和NP - 3的pK′a值分别为 8 5、7 6和 6 7。 相似文献
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Yang Ding Chenchen Xu Zheng Li Wenjing Qin Xisi Han Xu Han Prof. Chengwu Zhang Prof. Changmin Yu Prof. Xuchun Wang Prof. Lin Li Prof. Wei Huang 《Chembiochem : a European journal of chemical biology》2019,20(6):831-837
A fast-response fluorogenic probe—compound D1 —for monitoring hypochlorite (ClO−), based on specific ClO− cleavage of a C=N bond and producing results observable to the naked eye, has been developed. The response of the probe to ClO− increases linearly, and the fluorescence intensity was heightened by a factor of about 25. D1 responses to ClO−, with high selectivity and sensitivity, were observable by naked eye within 10 s. D1 can not only detect levels of hypochlorite in vitro, such as in urine, but is also capable of monitoring hypochlorite content under extremely cold conditions, as low as −78 °C. Meanwhile, its good biocompatibility permitted the use of D1 to detect intracellular ClO− by confocal microscopy. Moreover, D1 was successfully applied to monitor exogenous and endogenous ClO− in zebrafish through fluorescence imaging. 相似文献
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Thomas Pesnot Dr. Monica M. Palcic Prof. Gerd K. Wagner Dr. 《Chembiochem : a European journal of chemical biology》2010,11(10):1392-1398
Glycosyltransferases (GTs) are a large class of carbohydrate‐active enzymes that are involved, in both pro‐ and eukaryotic organisms, in numerous important biological processes, from cellular adhesion to carcinogenesis. GTs have enormous potential as molecular targets for chemical biology and drug discovery. For the full realisation of this potential, operationally simple and generally applicable GT bioassays, especially for inhibitor screening, are indispensable tools. In order to facilitate the development of GT high‐throughput screening assays for the identification of GT inhibitors, we have developed novel, fluorescent derivatives of UDP‐galactose (UDP‐Gal) that are recognised as donor analogues by several different retaining galactosyltransferases (GalTs). We demonstrate for one of these derivatives that fluorescence emission is quenched upon specific binding to individual GalTs, and that this effect can be used as the read‐out in ligand‐displacement experiments. The novel fluorophore acts as an excellent sensor for several different enzymes and is suitable for the development of a new type of GalT bioassay, whose modular nature and operational simplicity will significantly facilitate inhibitor screening. Importantly, the structural differences between the natural donor UDP‐Gal and the new fluorescent derivatives are minimal, and the general assay principle described herein may therefore also be applicable to other GalTs and/or proteins that use nucleotides or nucleotide conjugates as their cofactor. 相似文献
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以8-羟基喹啉衍生物与2,4-二羟基苯甲醛为原料,合成了一种新型的离子荧光探针HQHD。该化合物结构通过红外、氢谱、碳谱以及高分辨质谱进行确认。当以V(乙腈)∶V(水)=1∶9缓冲溶液为溶剂时,荧光探针的性能研究表明,在16种常见金属离子和NH~+_4中,HQHD对Co~(2+)具有较好的专一识别能力,p H在6.98~8.6范围内对Co~(2+)的识别能力最强。当Co~(2+)与其他离子形成共存离子溶液时,并不影响HQHD对Co~(2+)的检测效果,说明配体HQHD具有较强的抗干扰能力。当Co~(2+)在0.5×10~(-5)~5×10~(-5)mol/L的浓度范围内,体系的荧光强度ΔF与Co~(2+)的浓度呈现良好的线性关系,线性拟合系数为0.982 6,检测限为9.77×10~(-8)mol/L。Job's plot曲线表明,Co~(2+)与配体比为1∶1。 相似文献
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以4-二乙胺基水杨醛、三氟乙酰乙酸乙酯以及2-噻吩甲酰肼为原料合成了新型含氟香豆素类硫代酰腙型荧光探针化合物,经1HNMR、13CNMR、19FNMR、HR-MS对化合物结构进行了确认。通过荧光光谱法研究了化合物在乙腈溶液中对金属离子的识别性能。在pH 4.5~9.5的范围内,Hg^2+的加入使得化合物在464 nm处的荧光发射峰发生猝灭,其他金属离子的存在未对该现象造成明显干扰。Hg^2+浓度越高,猝灭效果越明显。因此,化合物对Hg^2+具有良好的荧光选择性和抗干扰性。Job′s曲线表明,化合物与Hg^2+的配合比为1∶1。 相似文献
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荧光传感器提供了方便,快捷,廉价的分析检测重金属离子的方法,并且有很高的灵敏度和选择性.它在环境科学,分析化学以及生命科学等领域有着广泛的应用前景.综述了分子内电荷转移(intramolecular charge transfer)荧光传感器的最新研究进展,并展望了该领域的发展趋势. 相似文献
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Shuai Xia Dr. Yibin Zhang Dr. Mingxi Fang Logan Mikesell Tessa E. Steenwinkel Shulin Wan Tyler Phillips Prof. Rudy L. Luck Prof. Thomas Werner Prof. Haiying Liu 《Chembiochem : a European journal of chemical biology》2019,20(15):1986-1994
We report a near-infrared fluorescent probe A for the ratiometric detection of cysteine based on FRET from a coumarin donor to a near-infrared rhodamine acceptor. Upon addition of cysteine, the coumarin fluorescence increased dramatically up to 18-fold and the fluorescence of the rhodamine acceptor decreased moderately by 45 % under excitation of the coumarin unit. Probe A has been used to detect cysteine concentration changes in live cells ratiometrically and to visualize fluctuations in cysteine concentrations induced by oxidation stress through treatment with hydrogen peroxide or lipopolysaccharide (LPS). Finally, probe A was successfully applied for the in vivo imaging of Drosophila melanogaster larvae to measure cysteine concentration changes. 相似文献