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By a sequentially selective extraction procedure, polysaccharides (PS) from cyanobacterium Spirulina (Arthrospira) maxima that can be bio-functionally healthy components were fractionated in four parts including the culture medium (PSCM), the
external layers of the cell (PSEL), the cell wall (PSCW) and storage granules (PSSG). The four fractionated parts of polysaccharides
were characterized by gas-liquid chromatography (GLC) equipped with FID and fused-silica capillary column (15 m×0.53 mm i.
d.). The contents of PSCM, PSEL, PSCW and PSSG were about 2.0%, 2.6%, 10.0% and 52.0% to cell dry matter, respectively. Glucose
was almost the only monosaccharide in PSSG and PSCW and most predominant in PSEL, while in PSCM, xylose, rhamnose and glucose
were the main ones. Two uronic acids represented by glucuronic acid and galacturoic acid, six neutral monosaccharides including
fucose, rhamnose, xylose, mannose, galactose and glucose, and two possible unidentified sugars were found in PSEL and PSCM. These data are valuable for the selective productions
of high-added value sugars from Spirulina.
Foundation item: Scholarship for Ph. D at FPMs, Belgium
Biography of the first author: NIE Zhen-yuan, Engineer in biomedicine, born in 1963, majoring in biotechnology and bioengineering. 相似文献