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本文目的是研究磷脂酶A1的固定化条件并将固定化酶用于脱胶过程。以磁性Fe3O4/SiOx-g-P(GMA)复合粒子为载体,考察不同的固定化条件对固定化酶相对酶活的影响,然后将制得的磁性固定化PLA1用于大豆油脱胶中,并对其酶活再生进行有益研究。结果表明,最优固定化条件为:酶液添加量20mL,pH6.0,反应时间5h,此时酶活力最高;最佳脱胶条件为:反应时间6h、反应温度55℃、加酶量0.12g/kg、油相起始pH6.0。在最优条件下进行脱胶试验,测定脱胶油中的磷含量为12.5mg/kg,脱胶效果较好。将再生的磁性固定化磷脂酶A1再用于大豆油脱胶中,延长了磁性固定化磷脂酶A1的利用时间,降低了大豆油酶法脱胶的生产成本。 相似文献
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在溶剂体系(正己烷)与无溶剂体系下对大豆油脱胶工艺进行了比较研究.结果表明:溶剂体系下,对大豆油脱胶效果影响最大的因素是油与溶剂比,其次是加水量,而反应温度、反应时间影响较小.将含溶剂大豆油(大豆油与正己烷质量比4.0∶1)预热至60c℃,添加6%(占油质量)的水,10 000 r/min下均质1 min,搅拌反应30 min后获得的脱胶油磷脂含量可降至136.0 mg/kg(磷含量5.2 mg/kg),脱胶率达99.5%,脱胶油酸值降低,甘一酯基本消失.无溶剂体系下添加酸类脱胶剂时脱胶油磷脂含量可降至58.6 mg/kg.与无溶剂体系相比,溶剂体系下获得的脱胶副产物磷脂色泽更浅,品质更好. 相似文献
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酶处理对菜籽油脱胶及品质的影响 总被引:2,自引:0,他引:2
采用磷脂酶A1(Lecitase ultra)对菜籽油进行脱胶实验,研究该酶对菜籽油脱胶及品质的影响。结果表明,Lecitase ultra在菜籽油脱胶中发挥了重要作用,能使菜籽油磷含量显著降低。通过正交试验得出Lecitase ultra应用于菜籽油脱胶的最佳工艺参数:pH值4.82、酶解温度37℃、加酶量150LU/kg油,油水混合物总含水量为2.69%,酶反应时间为3h,在此条件下脱胶油的磷含量为6.97mg/kg。同时,脱胶后菜籽油的酸价与过氧化值上升,色泽变浅,棕榈酸、油酸的含量下降,亚油酸、亚麻酸、花生一烯酸、芥酸的含量有所上升,其它几种脂肪酸的含量变化不大。 相似文献
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利用PLA1脱除大豆毛油中的磷脂,再用硅胶吸附脱除大豆油中的残磷和残皂。以酶法脱胶后含磷量、硅胶吸附脱皂后的含磷量和含皂量为评价指标,研究酶法脱胶及硅胶吸附脱皂对大豆油脱胶脱皂效果的影响。结果表明:在大豆油pH 5.5~6、去离子水添加量3%、反应时间6~8 h的脱胶条件下,酶法脱胶效果随PLA1添加量的增加而提高,PLA1添加量分别为50、75、100 mg/kg时,脱胶大豆油中含磷量分别从566.36、538.02、562.76 mg/kg降至44.67、18.99、17.01 mg/kg,再添加油质量0.1%的SORBSIL R92硅胶,大豆油含磷量分别降至41.21、16.35、15.42 mg/kg,含皂量分别从37、23、14 mg/kg降至14、8、5 mg/kg。酶法脱胶后大豆油酸值和过氧化值有所升高,3个油样的酸值(KOH)平均升高0.63 mg/g,过氧化值平均升高0.007 g/100 g。硅胶吸附脱皂也造成大豆油酸值和过氧化值有所升高。 相似文献
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利用实验室前期构建的重组大肠杆菌所产磷脂酶A1( p h o s p h o l i p a s e A 1, P L A 1)和磷脂酶C(phospholipase C,PLC)进行大豆油酶法脱胶研究,探讨自主开发重组酶进行酶法脱胶的可行性。以诺维信商品酶Lecitase Ultra™为对照,研究酶法脱胶反应温度、反应pH值、反应时间、搅拌速率、复合磷脂酶添加量工艺参数对大豆油脱胶的影响,并采用正交试验对脱胶工艺条件进行优化。研究结果表明,大豆油复合酶法脱胶的最佳工艺参数为:反应温度45 ℃、反应pH 6.5、反应时间3 h、搅拌速率300 r/min、PLA1和PLC添加量分别为7 940 U/kg和23 130 U/kg。复合磷脂酶对大豆油脱胶的效果与诺维信商品酶Lecitase Ultra™基本一致,大豆油磷含量可降至5 mg/kg以下,能够满足物理精炼的要求,为进一步开发具有知识产权的食品级油脂脱胶用酶制剂产品提供了理论依据。 相似文献
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Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides. 相似文献
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Sophie Guillot Laurence Peytavi Sylvie Bureau Renaud Boulanger Jean-Paul Lepoutre Jean Crouzet Sabine Schorr-Galindo 《Food chemistry》2006
The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties. 相似文献
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Jesus Simal-Gandara Miguel Sarria-Vidal Arjen Koorevaar Rinus Rijk 《Food Additives & Contaminants》2000,17(8):703-711
The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials. 相似文献
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M. S. Garcí a-Falc n J. Simal-G ndara S. T. Carril-Gonz lez-Barros 《Food Additives & Contaminants》2000,17(12):957-964
A simple, rapid and inexpensive method has been developed for the determination of benzo[a 相似文献
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H. J. Van Den Top A. Boenke P. A. Burdaspal J. Bustos H. P. Van Egmond T. Legarda A. Mesego A. Mourino W. E. Paulsch C. Salgado 《Food Additives & Contaminants》2001,18(9):810-824
This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance. 相似文献
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《肉类研究》2014,(2)
<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the 相似文献
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《印刷工业》2014,(11):95-95
According to Printing and Printing Equipment Industries Association of China(PEIAC)'s statistics to the plate manufucturer in China, in 2013, the actual offset plate production has reached 346 million square meters in China. Among them, the CTP production volume was 245 million square meters, up by 11% than that of last year; the total sales of the CTP plate was 239 million square meters, up by 13%. 相似文献
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《印刷工业》2014,(8):103-103
正Held at Guangdong Modern International Exhibition Center,Print China 2015 will cover 7exhibition halls,besides the original Hall No.3,4,5,6,7,the newly built F zone of Hall 3 will be used too.The total area will be140,000 square meters.Hall 3:Offset and large printing equipment,package printing equipment,post press 相似文献
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《印刷工业》2014,(12):102-103
Sponsored by Printing and Printing Equipment Industries Association of China (PEIAC) and organized by Print China magazine, the Seventeenth Beijing International Printing Information Conference (INFOPRINT 2014) was successfully held on 11th Dec. 2014 at Dongguan Exhibition International Hotel. 相似文献