一种新型包埋染料的核壳纳米颗粒表面增强共振拉曼探针的研制及其在免疫分析中的应用

应用于生物分析的纳米探针主要包括:等离子体共振颗粒探针,量子点、荧光探针和拉曼探针等等.拉曼探针跟其它纳米颗粒探针相比,其重要的优点是它能提供丰富的分子结构信息,从而很大程度上避免了光谱重叠,有可能研制出各种不同的拉曼探针.特别值得注意的是,基于表面增强与共振增强相结合的纳米拉曼探针,能超灵敏地、高特异性地检测生物分子.该文采用了一种新型的合成方法,成功地制备了以纳米金为核,二氧化硅为外壳,且其中包埋有染料的金/二氧化硅核壳纳米颗粒,并把它作为一种表面增强共振拉曼(SERRS)探针用于免疫分析.     

半导体荧光纳米颗粒的制备及其在生物传感器中的应用

该文综述了半导体荧光纳米颗粒及核-壳结构半导体纳米颗粒的制备、性能表征及其在生物传感器上的应用研究情况,共引用文献54篇.     

聚乙烯亚胺修饰的银纳米颗粒的制备研究及在肝素检测中的应用

该文利用分子量为600的高支化聚胺高分子PEI,在室温条件下,采用化学还原法,使用还原剂硼氢化钠,合成了银纳米颗粒。该方法具有操作简单、便捷、可重复性高等特点。对合成的颗粒进行了一系列的表征,包括紫外可见光谱的测量,动态光散射测量和透射电镜表征等。并以该银纳米颗粒为基础,建立了比色法传感器用于肝素的快速检测,并获得了较好的检测结果,检测限为1μg／mL。该检测方法具选择性高、灵敏度好,且检测耗时短,便于人眼直接观测等优点。     

金纳米颗粒制备及其光学特性研究

采用晶种生长法通过改变晶种对生长溶液的体积比例、引入离子合成了棒状和星形金纳米颗粒.首先用NaBH4作为还原剂制得小粒径球形金纳米颗粒作为晶种溶液,生长溶液中引入AgNO3为辅助试剂、溴化十六烷三甲基铵(CTAB)为表面活性剂来引导合成形貌不同的颗粒.对制备的球状、棒状和星状金纳米颗粒进行了紫外-可见光谱测试和表面增强...     

氮化硼纳米片/ 银纳米杂化颗粒填充的环氧树脂复合材料的制备及其性能研究

填充型高导热聚合物复合材料是目前解决电子器件散热问题的重要材料。基于此,该文通过液相剥离和化学还原法制备了氮化硼纳米片/银纳米粒子(BNNSs/AgNPs)杂化粒子,并以此为填料制备了 BNNSs/AgNPs/环氧树脂复合材料。前期研究工作证实通过 BNNSs/AgNPs 杂化粒子的填充,复合材料的导热性能得到了有效提高。然而,复合材料其他方面的综合性能也相当重要。因此,通过热失重、动态热机械性能以及介电性能测试对 BNNSs/AgNPs/环氧树脂复合体系的电学和力学性能进行考察和分析。结果表明,杂化粒子的填充对复合材料热分解温度有所提高,复合物的介电常数随着填料含量的增加而增加,BNNSs/AgNPs/环氧树脂的介电常数相对于 BNNSs/环氧树脂有进一步的提高。复合材料的储能模量和玻璃化转变温度随着填料含量的增加而升高。相对于 BNNSs,BNNSs/AgNPs 杂化粒子使得环氧树脂复合物的玻璃化转变温度进一步提高。BNNSs/AgNPs/环氧树脂复合材料良好的热学、力学和电学性能,能进一步满足聚合物基复合材料在现代电子器件和设备封装领域的要求。     

功能化硅壳磁性纳米颗粒亲和吸附介质靶向分离蛋白质研究

基于溴化氰修饰方法在硅壳磁性纳米颗粒表面修饰功能性生物分子,获得功能化硅壳磁性纳米颗粒亲和吸附介质.以胰蛋白酶-胰蛋白酶抑制剂作为模式亲和对,基于酶和抑制剂之间的特异性结合原理,首先考察了胰蛋白酶抑制剂的修饰对硅壳磁性纳米颗粒粒径和电位的影响及其修饰效率,然后利用胰蛋白酶抑制剂修饰的硅壳磁性纳米颗粒亲和吸附介质对简单模型蛋白质混合溶液以及胰脏组织中的胰蛋白酶进行靶向分离.该研究基于硅壳磁性纳米颗粒的内核磁性及表面生物修饰,为蛋白质分离提供了一种新型亲和吸附介质.     

金纳米颗粒的绿色制备及其在生物传感器中的应用

由于金纳米颗粒（AuNPs）特殊的化学、物理特性。使其在生物催化及传感器领域有了越来越重要的应用。目前用来制备金纳米材料的方法有很多。近年来采用新型的、洁净无毒的、绿色的方法来合成金纳米粒子越来越受到重视。     

纳米颗粒增强尿酸酶生物传感器的研究设想

文章简要介绍了尿酸酶生物传感器研究的必要性及国内外研究现状;阐述了纳米颗粒增强酶生物传感器的初步机理,并对国内外纳米颗粒增强葡萄糖氧化酶生物传感器的研究进展进行评述.本课题组设想并正在进行有关纳米颗粒增强尿酸酶生物传感器方面的探索研究.     

基于微通道的ZnO纳米棒生物荧光检测研究

利用种子法和水热合成技术,分别在常规条件下和阵列式微通道中制备氧化锌(ZnO)纳米棒.采用扫描电子显微镜(SEM)、X射线衍射(XRD)等分析方法表征ZnO纳米棒的表面形貌特点和晶体结构.结果表明:微通道中制备的ZnO纳米棒的比表面积、结晶度和c轴取向性均有较大程度的提高.同时,建立了基于阵列式微通道的ZnO纳米棒生物荧光检测方法,利用ZnO纳米棒可显著增强荧光信号,对异硫氰酸荧光素标记的羊抗牛IgG抗体的检测限为1×10-4 μg/mL.     

基于纳米金、铁氰化镍纳米颗粒共修饰的电流型甲胎蛋白免疫传感器的研究

该文通过铁氰化镍纳米颗粒和纳米金之间的化学作用及强的吸附作用,利用纳米金良好的生物兼容性和强烈的吸附作用固定甲胎蛋白抗体,最后用牛血清白蛋白封闭非特异性吸附位点,从而制备了一种新型的用于检测甲胎蛋白的电流型免疫传感器.在优化条件下,该传感器分别在1.0到10.0ng/mL和10.0到200.0 ng/mL两个浓度范围内对甲胎蛋白展示了良好的线性响应关系,检出限为0.2 ng/mL.     

Preparation of Ag–Au nanoparticle and its application to glucose biosensor

In this paper, Ag–Au nanoparticles are produced in sodium-bis(2-ethylhexyl)-sulfosuccinate (AOT)–cyclohexane reverse micelle system. The properties of the obtained nanoparticles are characterized with transmission electron microscope (TEM) and UV–vis absorption spectrophotometer. Glucose biosensors have been formed with glucose oxidase (GOx) immobilized in Ag–Au sol. GOx are simply mixed with Ag–Au nanoparticles and crosslinked with a polyvinyl butyral (PVB) medium by glutaraldehyde. Then a platinum electrode is coated with the mixture. The effects of the various molar ratios of Ag–Au particles with respect to the current response and the stability of the GOx electrodes are studied. The experimental results indicate the current response of the enzyme electrode containing Ag–Au sol increase from 0.32 to 19 μA cm⁻² in the solution of 10 mM β-d-glucose. In our study, the stability of enzyme electrodes is also enhanced.     

证书撤销机制的分析与研究

数字证书是实现电子政务和电子商务中实体的信任及信任验证的关键元素.CA实际可能会根据不同的情况而导致证书的意外作废或撤销,那么应使要使用证书的用户尽可能获知最新的证书情况,这对于实现PKI系统的可信性至关重要.通过分析国内外通常采用的CRL和OCSP这两种基本的证书撤销、查询方法,总结了它们的优缺点以及在实际应用过程中遇到的难点.最后提出了相应的改进措施,使用户能及时获得最新的证书状况,为电子政务和电子商务提供更可靠的安全性.     

Control mechanism of an organic self-regulating microfluidic system

The control mechanism and fluid dynamic properties of a previously developed organic pH regulation system are analyzed. The system regulates an output fluid stream to a pH of 6.7 with varying input flow rates. A pH sensitive hydrogel post acts as the feedback pH sensor and flow regulator. The control mechanism of the system is studied through numerical modeling of the regulator and the model is validated through experimentation. Analysis of the fluid dynamics at a T-channel junction, in which two buffer streams merge into one, is performed by solving the Navier-Stokes equation with commercial software. Various areas of a star-shaped orifice are occluded by a flexible membrane to throttle the rate that compensating buffer is fed back into the system. The relationship between orifice open area and volume of compensating buffer through the orifice was analyzed numerically. The axial and lateral visualization of the hydrogel post was obtained via optical microscopy. The model of the regulation system successfully predicts experimental results.     

Highly responsive fluorescent sensing of explosives taggant with an organic nanofibril film

Efficient sensing of an explosives taggant, 2,3-dimethyl-2,3-dinitrobutane (DMNB), has been performed with an organic nanofibril film through monitoring the fluorescence quenching of the film upon exposure to the DMNB vapor. The nanofibril film was fabricated from an alkoxycarbonyl-substituted carbazole-cornered conjugate tetracycle molecule, namely ACTC, which possesses a planar, rigid molecular geometry that favors cofacial π–π stacking between the molecules, leading to the formation of nanofibril structures with extended one-dimensional molecular stacking. The nanofibril film thus fabricated demonstrated unprecedented efficiency in detecting DMNB vapor, likely due to the extended one-dimensional molecular packing and the highly porous structure thus formed within the film. The former facilitates the exciton migration along the long-axis of nanofiber, while the latter enhances the adsorption of DMNB vapor and the expedient diffusion of the analyte through the film. The enhanced adsorption and diffusion of DMNB molecules within the matrix of the film also accounts for the improved response time of the film in fluorescence quenching upon exposure to DMNB vapor.     

ZnS掺Mn磷光量子点对金属离子传感机理的探讨

合成了以3-巯基丙酸或L-半胱氨酸修饰的水溶性ZnS掺Mn磷光量子点,磷光发射峰在590 nm,产生于Mn2+的4T1-6A1跃迁.磷光信号稳定,不易受溶解氧的影响.检测了七种常见金属离子对磷光性质的影响,结果表明:对于3-巯基丙酸修饰的ZnS:Mn量子点,Cu2+,Mn2+,Fe3+,Co2+,pb2和Hg2+离子能...     

White organic light-emitting diodes with improved performance using phosphorescent sensitizer and ultrathin fluorescent emitter

White organic light-emitting diodes (WOLEDs) have been fabricated by using a novel phosphor of bis(1,2-dipheny1-1H-benzoimidazole) iridium (acetylacetonate) [(pbi)₂Ir(acac)] as a sensitizer doped into a carbazole polymer of poly(N-vinylcarbazole) (PVK), and a fluorescent dye of 4-(dicyanomethylene)-2-t-butyl-6-(1,1,7,7-tetramethyljulolidyl-9-enyl)-4H-pyran(DCJTB) as an ultrathin red light-emitting layer. By characterizing the UV–Vis absorption spectra, photoluminescence (PL) spectra of (pbi)₂Ir(acac) and DCJTB, and the electroluminescence (EL) properties of the WOLEDs, the effect of ultrathin fluorescent light-emitting layer on the injection and transport characteristics of charge carrier and EL performance of WOLEDs was investigated. The results demonstrated that the device has stable EL spectra and Commissions Internationale de 1’Eclairage (CIE) coordinates in a wide bias range. Both energy transfer and charge trapping play the role on the performance of WOLEDs, and the device luminance was enhanced significantly with a maximum luminance of 9260 cd/m², which is about 70% higher than the codoped device.     

纳米三氧化钨制备技术及研究现状

纳米三氧化钨(WO3)作为一种典型的敏感材料已经广泛运用到了工业及民用生活等领域,日益受到研究者们的广泛关注。不同维度的纳米结构可有望得到性能优异的功能材料,通过结构设计及形貌改善可显著提高纳米WO3的气敏性能,本文综述了近年来不同形貌纳米WO3的制备技术及其在气敏传感器方面的研究现状,并指出了在研究过程中存在的问题。     

Structural,morphological and microhardness characterization in polymer complex of laser dye rhodamine (Rh6G) doped polymethyl methacrylate

Polymer systems have various contents of rhodamine (Rh6G) doped Polymethyl methacrylate (PMMA) prepared by solution cast method. The structural and morphological properties were studied by Differential Scanning calorimetry (DSC), Fourier Transform Infrared spectroscopy (FTIR) Scanning Electron Microscopy (SEM) and X-ray diffraction methods. The induced effect on the strength of rhodamine (Rh6G) doped Polymethyl methacrylate (PMMA) samples were studied by measuring the surface microhardness using the Vicker’s microhardness tester. Significant changes were observed in the Vickers microhardness number (H_v)     

掺杂荧光染料的高亮度有机蓝光电致发光器件

利用蓝色发光材料DPVBi掺杂高荧光染料rubrene做发光层制备了蓝色发光器件。在掺杂浓度为1.5%(wt)左右的情况下,当改变掺杂层的总的厚度时,器件的亮度、效率和色坐标都有明显的改变。当掺杂层DPVBi和rubrene的厚度为40nm,电子传输层Alq3的厚度为20nm,器件所加的电压是13v时,其最大亮度为14000cd/m2,此时的色坐标为(0.24,0.24),为蓝光发射。这种掺杂明显的提高了蓝光器件的发光效率。使最大效率达到2.5cd/A。     

掺杂荧光染料的高亮度有机蓝光电致发光器件

利用蓝色发光材料DPVBi掺杂高荧光染料rubrene做发光层制备了蓝色发光器件.在掺杂浓度为1.5%(wt)左右的情况下,当改变掺杂层的总的厚度时,器件的亮度、效率和色坐标都有明显的改变.当掺杂层DPVBi和rubrene的厚度为40nm,电子传输层Alq3的厚度为20nm,器件所加的电压是13v时,其最大亮度为14000cd/m2,此时的色坐标为(0.24,0.24),为蓝光发射.这种掺杂明显的提高了蓝光器件的发光效率.使最大效率达到2.5cd/A.