Effects of replacing roughage with soy hulls on feeding behavior and milk production of dairy cows under hot weather conditions

Two total mixed rations (TMR) containing different proportions of roughage neutral detergent fiber (NDF) were fed to lactating cows under Israeli summer conditions, and the effects on feeding behavior and milk production were measured. Forty-two lactating cows were divided into 2 groups fed ad libitum a TMR containing either 18% NDF of roughage origin (control group) or only 12% roughage NDF, in which the corn silage component (16.5% of dry matter [DM]) was replaced with soy hulls (experiment group). This and additional adjustments in TMR were reflected in higher net energy for lactation and in vitro digestibility of the experimental TMR. Cow behavior was investigated at the feeding lane during June 2001; about 11,000 cow visits were analyzed. Feed intake per meal and average meal duration were significantly higher in the experiment group (1.51 kg of DM per meal and 12.1 min per meal, respectively) as compared with the control group (1.22 kg of DM per meal and 9.47 min per meal, respectively). However, number of meals per day recorded in the feeding lane was significantly higher in the control group (21.0 vs. 16.6 meals/d per cow). Distribution of meals and feed intake along the day depended more on management practices, such as milking and feed dispensing times, than on feed composition or weather conditions. These differences between groups were expressed in similar daily eating duration (approximately 200 min), and because the rate of feed consumption was similar for both treatments (approximately 127 g DM/min), the daily average DM intake was also similar (25.0 to 25.7 kg). However, NDF intake was higher in the experiment group. Consequently, the average milk yield was higher in the experimental group, and production of milk fat, 4% fat-corrected milk, and economically corrected milk were significantly higher in the experiment group than in the control group. Data imply that the experimental TMR containing only 12% NDF of roughage origin is more suitable for cows under hot climate conditions compared with the control TMR.     

Method of diet delivery to dairy cows: Effects on nutrient digestion,rumen fermentation,methane emissions from enteric fermentation and stored manure,nitrogen excretion,and milk production

This study was conducted to examine the effect of method of diet delivery to dairy cows on enteric CH₄ emission, milk production, rumen fermentation, nutrient digestion, N excretion, and manure CH₄ production potential. Sixteen lactating cows were used in a crossover design (35-d period) and fed ad libitum twice daily a diet [52:48, forage:concentrate ratio; dry matter (DM) basis] provided as forages and concentrates separately (CF) or as a total mixed ration (TMR). For the CF treatment, concentrates were offered first followed by mixed forages 45 min afterward. Method of diet delivery had no effect on DM intake, but neutral detergent fiber (NDF) intake was greater when the diet was delivered as TMR as compared with CF. Apparent total-tract digestibility of DM, crude protein, and gross energy was slightly (1 percentage unit) lower when the diet was offered as TMR than when offered as CF. In contrast, NDF digestibility was greater when the cows were fed TMR versus CF. Although average daily ruminal pH was not affected by method of diet delivery, daily duration of ruminal pH <5.6 was less when the diet was delivered as TMR as compared with CF (0.9 h/d versus 3.7 h/d). Delivering the diet as TMR increased ruminal total volatile fatty acid and NH₃ concentrations, but had no effect on acetate, propionate, or branched-chain volatile fatty acid molar proportions. Yields of milk, milk fat, or milk protein, and milk production efficiency (kg of milk/kg of DM intake or g of N milk/g of N intake) were not affected by the method of diet delivery. Daily production (g/d), yield (% gross energy intake), and emission intensity (g/kg of energy-corrected milk) of enteric CH₄ averaged 420 g/d, 4.9%, and 9.6 g/kg and were not affected by diet delivery method. Fecal N output was greater when the diet was delivered as TMR versus CF, whereas urinary N excretion (g/d, % N intake) was not affected. Manure volatile solids excretion and maximal CH₄ production potential were not affected by method of diet delivery. Under the conditions of this study, delivering the diet as concentrates and forages separately versus a total mixed ration had no effect on milk production, enteric CH₄ energy losses, urinary N, or maximal manure CH₄ emission potential. However, feeding the diet as total mixed ration compared with feeding concentrates and forages separately attenuated the extent of postprandial decrease in ruminal pH, which has contributed to improving NDF digestibility.     

Digestibility in lactating cows of diets containing whole Pima treated with sodium hydroxide versus Akala or Pima cottonseed

This study examines the effect of NaOH treatment on the nutritive value of Pima cottonseed for lactating cows. Treatment of Pima cottonseed with NaOH increased the extent of dry matter (DM) and neutral detergent fiber in vitro digestibility. Three groups of cows were fed 3 similar total mixed rations (TMR) differing only in the type of cottonseed fed, either whole linted Akala, whole unlinted Pima, or whole Pima treated with 4% NaOH (T-Pima). Dry matter and organic matter (OM) intakes of Akala and Pima TMR were similar and tended to be lower compared with cows fed T-Pima. This was reflected in similar intake of crude protein and lipids by cows fed the 3 TMR. Digestibility of DM, OM, and crude protein were similar in cows fed Pima and T-Pima diets, and slightly higher in cows fed the Akala TMR. Lipid digestibility was highest in cows fed Akala TMR, whereas cellulose and NDF digestibility were lowest in Pima TMR. In the 3 TMR, there was a large gap between the high values of "digestibility of intact seeds" and actual in vivo digestion of DM and OM. Similar yields of milk and milk protein were obtained in cows fed the Akala and Pima TMR, whereas higher yields of milk, milk protein, milk fat, and 4% fat-corrected milk were found in the T-Pima cows. Similar efficiency of DM intake for fat-corrected milk production was achieved by cows fed the 3 TMR.     

Effects of timing of palmitic acid supplementation during early lactation on nutrient digestibility,energy balance,and metabolism of dairy cows

The objective of our study was to evaluate the effects of timing of palmitic acid (C16:0) supplementation during early lactation on nutrient digestibility, energy intake and balance, and metabolic responses of dairy cows. Fifty-two multiparous cows were used in a randomized complete block design experiment. During the fresh (FR) period (1–24 d in milk) cows were assigned to either a control diet containing no supplemental fat (CON) or a C16:0-supplemented diet [PA; 1.5% of diet dry matter (DM)]. During the peak (PK) period (25–67 d in milk) cows were assigned to either a CON diet or a PA diet (1.5% of diet DM) in a 2 × 2 factorial arrangement of treatments considering the diet that they received during the FR period. During the FR period, compared with CON, PA increased DM digestibility by 3.0 percentage units and neutral detergent fiber (NDF) digestibility by 4.4 percentage units, and the increase in these variables was consistent over time. Although PA did not affect 18-carbon fatty acid (FA) digestibility, it decreased 16-carbon FA digestibility by 10.8 percentage units and total FA digestibility by 4.7 percentage units compared with CON. We observed a tendency for an interaction between treatment and time for total FA digestibility and 16-carbon FA digestibility due to the difference in FA digestibility between PA and CON reducing over time. Compared with CON, PA increased digestible energy intake by 3.9 Mcal/d, metabolizable energy intake by 3.5 Mcal/d, and net energy for lactation intake by 2.5 Mcal/d. The PA diet also increased milk energy output, negative energy balance, and plasma nonesterified fatty acid concentration and reduced plasma insulin concentration. We also observed a tendency for an interaction between treatment and time for energy balance due to cows receiving the PA treatment being in a greater negative energy balance over time. During the PK period, PA increased DM digestibility by 2.9 percentage units and NDF digestibility by 3.5 percentage units compared with CON. Although PA decreased 16-carbon FA digestibility by 7.0 percentage units, PA did not affect 18-carbon FA digestibility or total FA digestibility. Feeding PA during the PK period increased energy intake and milk energy output and did not affect energy balance. In conclusion, feeding a C16:0 supplement to early-lactation cows consistently increased DM and NDF digestibilities and energy intake compared with a control diet containing no supplemental fat. Feeding C16:0 markedly increased milk energy output in both the FR and PK periods but increased negative energy balance only in the FR period.     

Effect of fiber content and particle size of alfalfa silage on performance and chewing behavior.

A low NDF drought-stressed 1988 alfalfa silage (32.6% NDF) and a higher fiber 1988 alfalfa silage (46.4% NDF) were fed to lactating cows to evaluate effects on feed intake, fat test, and chewing behavior. Two groups of Holstein cows, 16 primiparous housed in tie stalls and 16 multiparous in free stalls, were assigned to diets based on parity and milk yield. The low NDF silage was fed for 6 wk in a TMR with 21.5% NDF and was compared with a TMR with 31.9% NDF. During an additional 4-wk period, one-half of each dietary group was fed a ration in which one-half of each silage was rechopped to reduce particle size. All rations contained a 1:1 ratio of forages to concentrates (DM basis) and were fed for ad libitum intake. Diets with 21.5% NDF and reduced particle size had no influence on milk fat percentage, 4% FCM yield, or plasma glucose. Cows fed these diets had reduced chewing time, due largely to decreased rumination time. Rumination and total chewing times per unit DMI and FCM also were lowest on these diets. Intake of DM on a BW basis was lowest for cows fed the low NDF rechopped silage diet. Cows fed in tie stalls had more eating bouts than those in free stalls, but total eating times were similar. Sufficient amounts of effective fiber appeared to be present in low NDF and rechopped silage diets to prevent the systemic events leading to milk fat depression but not to prevent a reduction in chewing time.     

The Effect of Silage and Concentrate Type on Intake Behavior, Rumen Function, and Milk Production in Dairy Cows in Early and Late Lactation

The objective of this experiment was to evaluate the effect of feeding total mixed rations (TMR) that differ in structural and nonstructural carbohydrates to dairy cows in early and late lactation on short-term feed intake, dry matter intake (DMI), rumen fermentation variables, and milk yield. A 5 × 5 Latin square experiment with 15 dairy cows was repeated during early and late lactation. The 5 treatments were a TMR with (all on dry matter basis) 55% roughage (a 50:50 mixture of corn silage and grass silage) and 45% concentrate (a 50:50 mixture of concentrate rich in structural carbohydrates and concentrate rich in nonstructural carbohydrates; treatment CON), a TMR with the concentrate mixture and 55% grass silage (RGS) or 55% corn silage (RCS), and a TMR with the roughage mixture and 45% of the concentrate rich in structural carbohydrates (CSC) or the concentrate rich in nonstructural carbohydrates (CNS). Meal criteria, determined using the Gaussian-Gaussian-Weibull method per animal per treatment, showed an interaction between lactation stage and treatment. Feed intake behavior variables were therefore calculated with meal criteria per treatment-lactation stage combination. Differences in feed intake behavior were more pronounced between treatments differing in roughage composition than between treatments differing in concentrate composition, probably related to larger differences in chemical composition and particle size between corn silage and grass silage than between the 2 concentrates. The number of meals was similar between treatments, but eating time was greater in RGS (227 min/d) and lesser in RCS (177 min/d) than the other treatments. Intake rate increased when the amount of grass silage decreased, whereas meal duration decreased simultaneously. These effects were in line with a decreased DMI of the RGS diet vs. the other treatments, probably related to the high neutral detergent fiber (NDF) content. However, this effect was not found in CSC, although NDF content of the TMR, fractional clearance rate of NDF, and fractional degradation rate of NDF was similar between CSC and RGS. Rumen fluid pH was lesser, and molar proportions of acetic acid and of propionic acid were lesser and greater, respectively, in RCS compared with all other diets. Milk production did not differ between treatments. There was no effect of type of concentrate on milk composition, but diet RCS resulted in a lesser milk fat content and greater milk protein content than diet RGS. Lactation stage did affect short-term feed intake behavior and DMI, although different grass silages were fed during early and late lactation. The results indicate that short-term feed intake behavior is related to DMI and therefore may be a helpful tool in optimizing DMI and milk production in high-production dairy cows.     

Effects of including NaOH-treated corn straw as a substitute for wheat hay in the ration of lactating cows on performance,digestibility, and rumen microbial profile

This study measured the effects of including 5% NaOH-treated corn straw (T-CS) as a substitute for 15% wheat hay in the control total mixed ration (TMR) of lactating cows on performance, digestibility, and rumen microbial profile. Two groups of 21 cows each, similar in initial performance, were fed individually 1 of the 2 TMR examined. Voluntary dry matter intake of cows fed the control TMR was 4.3% higher than that of the T-CS cows, but in vivo dry matter and organic matter digestibilities of both groups were similar. Crude protein digestibility was higher in the control cows but digestibility of neutral detergent fiber polysaccharides (cellulose and hemicelluloses) was higher in the T-CS TMR. This was followed by 4.6% reduction in rumination time of the T-CS group. A slightly higher milk yield was observed in the control cows compared with the T-CS group; however, milk fat and milk protein content were higher in cows fed the T-CS TMR. This was reflected in 1.3% increase in energy-corrected milk yield and 5.34% increase in production efficiency (energy-corrected milk yield/intake) of the T-CS cows compared with the control. Welfare of the cows, as assessed by length of daily recumbence time, was improved by feeding the T-CS TMR relative to the control group. As a whole, the rumen bacterial community was significantly modulated in the T-CS group in the experimental period compared with the preexperimental period, whereas the bacterial community of the control group remained unchanged during this period. Out of the 8 bacterial species that were quantified using real-time PCR, a notable decrease in cellulolytic bacteria was observed in the T-CS group, as well as an increase in lactic acid-utilizing bacteria. These results illustrate the effect of T-CS on the composition of rumen microbiota, which may play a role in improving the performance of the lactating cow.     

Effects of alfalfa particle size and specific gravity on chewing activity, digestibility, and performance of Holstein dairy cows

Two experiments were carried out to test the effects of alfalfa particle size and functional specific gravity (FSG) on chewing activity, digestibility, rumen kinetics, and production of lactating dairy cows fed corn silage based rations. In experiment 1, water-holding capacity (WHC), insoluble dry matter, hydration rate, and FSG changes were determined in alfalfa hay (varying in particle size) and corn silage. Reduction of particle size increased bulk density, FSG, and the rate of hydration, and decreased WHC of alfalfa. In experiment 2, 9 midlactation Holstein dairy cows fed total mixed rations containing 3 sizes of alfalfa hay (with geometric mean 7.83, 4.04, and 1.14 mm) were used in a replicated 3 x 3 Latin square design. The diets contained 20, 20, 35, 7, 7.5, 10, 0.3, 0.1, and 0.1% of DM alfalfa, corn silage, barley, soybean meal, beet pulp, wheat bran, dicalcium phosphate, vitamin premix, and salt, respectively. The geometric means (GM) of rations were 3.34, 2.47, and 1.66 mm in long, medium, and fine alfalfa treatments, respectively. Reduction of particle size increased daily NDF intake (kg), but decreased the proportion of physically effective factor (pef) and physically effective NDF (peNDF) in the ingested rations. Reduction of particle size increased the FSG of rations and intake of DM but reduced digestibility of NDF and ash. Reduction of particle size decreased ruminal mean retention time (RMRT), but increased the ruminal particulate passage rate. Milk and FCM yield were not affected by treatments. The rumen pH, total chewing activity, rumination, eating time, and milk fat were reduced as particle size decreased, but milk protein increased. This study showed that reduction of forage particle size increased bulk density, FSG, and hydration rate of alfalfa and was the most influential factor affecting DMI, milk composition, and chewing behavior. Reduction of forage particle size had minimal impact on digestibility and milk production.     

The effects of source and concentration of dietary fiber,starch, and fatty acids on the daily patterns of feed intake,rumination, and rumen pH in dairy cows

The daily patterns of feed intake and rumination influence rumen fermentation, rumen pH, and timing of absorbed nutrients in the dairy cow, but the effects of diet composition on these patterns are not well characterized. Data from 3 previously published experiments were examined to determine the influence of dietary starch, fiber, and fatty acids (FA) on daily patterns of intake, rumination, and rumen pH. Dietary neutral detergent fiber (NDF) and starch were investigated in 2 experiments, each with duplicated 4 × 4 Latin square designs with a 2 × 2 factorial arrangement of treatments in cows fed cows 1×/d at 1200 and 1400 h, respectively. To investigate fiber content and digestibility in the first experiment, brown midrib or isogenic conventional corn silage were fed in low- and high-NDF diets (29 and 38%, respectively). To investigate starch source and concentration in the second experiment, ground high-moisture corn or dry ground corn were fed in low- and high-starch diets (21 and 32%, respectively). Effect of fat concentration and saturation was investigated in the third experiment using a replicated 4 × 4 Latin square design that fed cows 1×/d at 0900 h; treatments included a control diet with no added fat and 2.5% added saturated FA, unsaturated FA, or a mixture of the saturated and unsaturated FA. In the first 2 experiments, intake followed a similar daily pattern regardless of starch and NDF concentration or digestibility. Rumination displayed a treatment by time interaction for both NDF and starch concentration, with high-fiber, low-starch diets causing greater rumination overnight but not midday. High-starch diets decreased total daily rumen pH equally across the day, but did not change the daily pattern. Type of corn silage did not affect the daily patterns of rumination or rumen pH, but pH was reduced throughout the day in brown midrib diets. In the third experiment, no interactions between fatty acid supplement and time of day were observed for intake, rumination, or rumen pH. Within all experiments, rumination fit or tended to fit a 24-h rhythm regardless of diet, with the amplitude of the rumination being reduced in low-starch diets and diets containing saturated FA or a mixture of saturated and unsaturated FA. Overall, intake, rumination, and rumen pH follow a daily pattern that was minimally modified by dietary fiber and starch type and level or fat level and fatty acid profile.     

Effects of a commercial fermentation byproduct or urea on milk production,rumen metabolism,and omasal flow of nutrients in lactating dairy cattle

The objective of this study was to evaluate the effects of a fermentation byproduct on rumen fermentation and microbial yield in high producing lactating dairy cattle. Eight ruminally cannulated multiparous Holstein cows averaging (mean ± standard deviation) 60 ± 10 d in milk and 637 ± 38 kg of body weight were assigned to 1 of 2 treatment sequences in a switchback design. Treatment diets contained (dry matter basis) 44% corn silage, 13% alfalfa silage, 12% ground corn, and 31% premix containing either a control mix of urea and wheat middlings (CON) or a commercial fermentation byproduct meal (Fermenten, Arm and Hammer Animal Nutrition, Princeton, NJ) at 3% diet inclusion rate (EXP). Diets were formulated to be isonitrogenous and isocaloric, with similar levels of neutral detergent fiber and starch. The trial consisted of three 28-d experimental periods, where each period consisted of 21 d of diet adaptation and 7 d of data and sample collection. Omasal nutrient flows were determined using a triple-marker technique and double-labeled ¹⁵N¹⁵N-urea. The EXP diet provided 18 g/d more nonammonia N versus the CON diet, representing 3.0% of total N intake. Energy-corrected milk yield (41.7 and 43.1 kg/d for CON and EXP, respectively), milk fat, and protein yield and content did not differ between treatments. Total dry matter intake was similar between treatments (25.5 and 26.4 kg/d for CON and EXP, respectively). Ammonia N concentration and pool size in the rumen was greater in cows fed the EXP diet. No differences were observed in rumen or total-tract dry matter, organic matter, or neutral detergent fiber digestibility. Ruminal degradation of feed N was 15% lower in cows fed EXP diets, resulting in differences in omasal N flows. Results demonstrated the fermentation byproduct meal had a sparing effect on degradable feed protein, but did not increase microbial N flow from the rumen.     

Influence of fiber degradability of corn silage in diets with lower and higher fiber content on lactational performance,nutrient digestibility,and ruminal characteristics in lactating Holstein cows

The effect of neutral detergent fiber (NDF) degradability of corn silage in diets containing lower and higher NDF concentrations on lactational performance, nutrient digestibility, and ruminal characteristics in lactating Holstein cows was measured. Eight ruminally cannulated Holstein cows averaging 91 ± 4 (standard error) days in milk were used in a replicated 4 × 4 Latin square design with 21-d periods (7-d collection periods). Dietary treatments were formulated to contain either conventional (CON; 48.6% 24-h NDF degradability; NDFD) or brown midrib-3 (BM3; 61.1% 24-h NDFD) corn silage and either lower NDF (LNDF) or higher NDF (HNDF) concentration (32.0 and 35.8% of ration dry matter, DM) by adjusting the dietary forage content (52 and 67% forage, DM basis). The dietary treatments were (1) CON-LNDF, (2) CON-HNDF, (3) BM3-LNDF, and (4) BM3-HNDF. Data were analyzed as a factorial arrangement of diets within a replicated Latin square design with the MIXED procedure of SAS (SAS Institute Inc., Cary, NC) with fixed effects of NDFD, NDF, NDFD × NDF, period(square), and square. Cow within square was the random effect. Time and its interactions with NDFD and NDF were included in the model when appropriate. An interaction between NDFD and NDF content resulted in the HNDF diet decreasing dry matter intake (DMI) with CON corn silage but not with BM3 silage. Cows fed the BM3 corn silage had higher DMI than cows fed the CON corn silage, whereas cows fed the HNDF diet consumed less DM than cows fed the LNDF diet. Cows fed the BM3 diets had greater energy-corrected milk yield, higher milk true protein content, and lower milk urea nitrogen concentration than cows fed CON diets. Additionally, cows fed the BM3 diets had greater total-tract digestibility of organic matter and NDF than cows fed the CON diets. Compared with CON diets, the BMR diets accelerated ruminal NDF turnover. When incorporated into higher NDF diets, corn silage with greater in vitro 24-h NDFD and lower undegradable NDF at 240 h of in vitro fermentation (uNDF240) allowed for greater DMI intake than CON. In contrast, for lower NDF diets, NDFD of corn silage did not affect DMI, which suggests that a threshold level of inclusion of higher NDFD corn silage is necessary to observe enhanced lactational performance. Results suggest that there is a maximum gut fill of dietary uNDF240 and that higher NDFD corn silage can be fed at greater dietary concentrations.     

Effect of palmitic acid-enriched supplements containing stearic or oleic acid on nutrient digestibility and milk production of low- and high-producing dairy cows

We evaluated the effects of fatty acid (FA) supplement blends containing 60% palmitic acid (C16:0) and either 30% stearic acid (C18:0) or 30% oleic acid (cis-9 C18:1) on nutrient digestibility and milk production of low- and high-producing dairy cows. Twenty-four multiparous Holstein cows [118 ± 44 d in milk (DIM)] were divided into 2 blocks by milk production and then randomly assigned to treatment sequence in four 3 × 3 Latin squares within production level, balanced for carryover effects in three consecutive 21-d periods. Cows were blocked by milk yield and assigned to 1 of 2 groups (n = 12 per group): (a) low group (42.5 ± 3.54 kg/d; 147 ± 42 DIM) and (b) high group (55.8 ± 3.04 kg/d; 101 ± 34 DIM). Commercially available fat supplements were combined to provide treatments that consisted of the following: (1) control (CON; diet with no supplemental FA), (2) FA supplement blend containing 60% C16:0 and 30% C18:0 (PA+SA), and (3) FA supplement blend containing 60% C16:0 and 30% cis-9 C18:1 (PA+OA) The FA blends were fed at 1.5% of dry matter (DM) and replaced soyhulls from CON. Preplanned contrasts were (1) overall effect of FA treatments [CON vs. the average of the FA treatments (FAT); 1/2 (PA+SA + PA+OA)], and (2) effect of FA supplement (PA+SA vs. PA+OA). Regardless of production level, overall FAT reduced DMI compared with CON. Also, regardless of level of milk production, PA+OA increased total-tract FA digestibility compared with PA+SA. Treatment by production level interactions were observed for neutral detergent fiber (NDF) digestibility, total FA intake, and the yields of 3.5% fat-corrected milk (FCM), energy-corrected milk (ECM), and milk fat. In low-producing cows, FAT increased DM and NDF digestibility compared with CON. In high-producing cows PA+SA increased DM and NDF digestibility compared with PA+OA. In low-producing cows, PA+SA increased 3.5% FCM, ECM, and milk fat yield compared with PA+OA. However, in high-producing cows PA+OA tended to increase 3.5% FCM compared with PA+SA. In conclusion, low-producing cows responded better to a FA blend containing 60% C16:0 and 30% C18:0, whereas high-producing dairy cows responded more favorably to a FA blend containing 60% C16:0 and 30% cis-9 C18:1. However, further research is required to validate our observations that higher-yielding cows have improved production responses when supplemented with cis-9 C18:1 compared with C18:0.     

Diet supplementation with thyme oil and its main component thymol failed to favorably alter rumen fermentation,improve nutrient utilization,or enhance milk production in dairy cows

Phenolic compounds and essential oils with high content of phenolic compounds have been reported to exert antimicrobial activities in vitro. The objective of this study was to determine the effects of dairy cow diet supplementation with thyme oil and its main component thymol on intake and total-tract apparent digestibility of nutrients, rumen fermentation characteristics, ruminal protozoa, nitrogen excretion, and milk production. For this aim, we used 8 multiparous, ruminally cannulated Holstein cows in a replicated 4 × 4 Latin square design (28 d periods), balanced for residual effects. Cows were fed 1 of the 4 following experimental treatments: total mixed ration (TMR) with no additive (control); TMR + monensin [24 mg/kg of dry matter (DM)]; TMR + thyme oil (50 mg/kg of DM); and TMR + thymol (50 mg/kg of DM). Compared with the control diet, feeding thyme oil or thymol had no effect on DM intake, nutrient total-tract apparent digestibility, total N excretion, ruminal pH, ammonia concentration, total volatile fatty acid (VFA) concentration, or acetate:propionate ratio. Ruminal protozoa density was not modified by thyme oil, but decreased with thymol supplementation. Supplementation with thyme oil or thymol did not affect milk production, milk composition, or efficiency of milk production. Neither thyme oil nor thymol affected efficiency of dietary N use for milk N secretion (N intake/milk N). Supplementation with monensin tended to decrease DM intake (–1.2 kg/d) and milk fat yield. Total-tract apparent digestibility of nutrients did not differ between cows fed monensin and cows fed the control diet. Total VFA concentration was not changed by monensin supplementation compared with control, but adding monensin shifted the VFA profile toward more propionate and less acetate, resulting in a decrease of acetate:propionate ratio. Protozoa density and ammonia concentration were lower in the ruminal content of cows fed monensin compared with that of cows fed the control diet. Total N excretion was not affected by monensin supplementation. Likewise, efficiency of use of dietary N for milk N secretion was unchanged in cows fed monensin. The results of this study contrasted with the claimed in vitro antimicrobial activity of thyme oil and thymol: we observed no positive effects on rumen metabolism (i.e., N and VFA) or milk performance in dairy cows. Under the conditions of this study, including thyme oil or thymol at 50 mg/kg of DM had no benefits for rumen fermentation, nutrient utilization and milk performance in dairy cows.     

Increasing doses of carbohydrases: Effects on rumen fermentation,nutrient digestibility,and performance of mid-lactation cows

The objective of this study was to evaluate the effects of exogenous enzymes on nutrient intake and digestibility, rumen fermentation, and productivity of mid-lactating cows. Experiment 1 was designed to test increasing doses [0, 0.5, 1.0, or 1.5 g/kg of dry matter (DM)] of a combination of 2 enzyme products with xylanase and β-glucanase activities (Ronozyme Wx and Ronozyme VP, respectively; DSM Nutritional Products) on rumen fermentation and total apparent digestibility. Enzyme combinations had a ratio of endo-1,3(4)-β-glucanase to endo-1,4-β-xylanase of 8:2 (wt/wt). For experiment 1, 8 rumen cannulated lactating cows were used into a double 4 × 4 Latin square design experiment with 14 d of diet adaptation and 7 d of sampling. Despite no differences in feed intake, carbohydrases linearly increased neutral detergent fiber digestibility. Treatments marginally affected rumen fermentation, where a linear trend for lower rumen pH and a linear trend for greater isobutyrate concentration were observed with increasing enzyme dose. A trend for lower rumen NH₃-N concentration was observed for cows receiving carbohydrases in comparison with control group. When comparing all enzyme treatments against control group, cows fed enzymes tended to produce more 3.5% fat-corrected milk (FCM), produced more milk fat, and had greater blood glucose concentration. Experiment 2 evaluated 3 doses (0, 0.5, or 0.75 g/kg of DM) of the same combination of enzyme products on performance of cows (n = 36) in a complete randomized block (n = 12) design. Cows received treatments for 9 wk. No interaction effects between treatments and time were observed for all variables assessed in this study. In agreement with experiment 1, no differences were detected for feed intake, but cows fed the enzyme products tended to produce more 3.5% FCM and milk fat compared with control. In addition, cows fed enzymes exhibited greater efficiency of FCM production (FCM ÷ DM intake) compared with control. No differences were detected for intake and productivity when comparing the 2 doses of carbohydrases. In summary, the enzyme products tested in this study may improve feed efficiency due to greater milk fat concentration.     

Effect of dietary roughage level on chewing activity,ruminal pH,and saliva secretion in lactating Holstein cows

Increasing dietary roughage level is a commonly used strategy to prevent subacute ruminal acidosis. We hypothesized that high-roughage diets could promote chewing activity, saliva secretion, and hence more alkaline to buffer rumen pH. To verify the hypothesis, 12 multiparous Holstein cows in mid lactation were randomly allocated to 4 treatments in a triplicated 4 × 4 Latin square experiment with one cow in each treatment surgically fitted with a ruminal cannula. Treatments were diets containing 40, 50, 60, or 70% of roughage on a DM basis. Increasing dietary roughage level decreased DM, CP, OM, starch, and NE_L intake, increased ADF intake, and decreased milk yield linearly. Intake of NDF was quite stable across treatments and ranged from 7.8 to 8.1 kg/d per cow. Daily eating time increased linearly with increased roughage level. The increase in eating time was due to increased eating time per meal but not number of meals per day, which was stable and ranged from 8.3 to 8.5 meals per day across treatments. Increasing dietary roughage level had no effect on ruminating time (min/d), the number of ruminating periods (rumination periods per d), and chewing time per ruminating period (min/ruminating period). Ruminating time per kilogram of NDF intake and total chewing time per kilogram of ADF intake were similar across treatments (57.4 and 183.8 min/kg, respectively). Increasing dietary roughage level linearly increased daily total chewing time; linearly elevated the mean, maximum, and minimum ruminal pH; and linearly decreased total VFA concentration and molar proportion of propionate in ruminal fluid. Saliva secretion during eating was increased, the secretion during rumination was unaffected, but the secretion during resting tended to decrease with increased dietary roughage level. As a result, total saliva secretion was not affected by treatments. In conclusion, the results of the present study did not support the concept that high-roughage diets elevated ruminal pH through increased salivary recycling of buffering substrates.     

Effects of replacing grass silage with barley silage in dairy cow diets

This study examined the effects of gradually replacing grass silage with whole-crop barley silage on feed intake, ruminal and total tract digestibility, and milk yield in lactating dairy cows. Four dairy cows in early lactation, equipped with rumen cannulas, were fed 4 diets over four 21-d periods. The diets consisted of 4 forage mixtures of grass silage and whole-crop barley silage supplemented with 8.9 kg/d of concentrates [dry matter (DM) basis]. The proportion of barley silage in the forage was adjusted to 0, 0.20, 0.40, and 0.60 kg/kg of DM. Ruminal nutrient metabolism was measured on the basis of digesta flow entering the omasal canal. Ammonia concentrations and volatile fatty acid profiles were determined in the rumen fluid. Ruminal digestion and passage kinetics were assessed by the rumen evacuation technique. Replacement of grass silage with barley silage had no effect on DM, digestible organic matter, or neutral detergent fiber (NDF) intake, but starch intake increased, whereas nitrogen and digestible NDF (dNDF) intake decreased. Increases in the proportion of barley silage linearly decreased milk yield, and the molar proportion of acetate in the rumen, and increased that of propionate, butyrate, and valerate. Decreases in milk yield due to inclusion of barley silage were attributed to decreases in diet digestibility and nutrient supply to the animal. Barley silage linearly decreased organic matter digestibility in the total tract and NDF and dNDF digestibility in the rumen and the total tract, and decreased nonammonia N flow entering the omasal canal. No significant differences between diets were noted in the digestion rate of dNDF or passage rate of indigestible NDF from the rumen. Decreases in organic matter and NDF digestibility were attributed to the higher indigestible NDF concentration of barley silage compared with that of grass silage and to the smaller pool size of dNDF in the rumen.     

Meta-analysis of rumination behavior and its relationship with milk and milk fat production,rumen pH,and total-tract digestibility in lactating dairy cows

Time spent ruminating is affected by diet and affects the rumen environment. The objective of the current study was to conduct a meta-regression to characterize the variation in rumination time and its relationship with milk and milk fat yields and variables mechanistically associated with milk fat synthesis, including rumen pH and total-tract digestibility. The analysis included 130 journal articles published between 1986 and 2018 that reported 479 treatment means from lactating Holsteins cows during established lactation. Milk yield averaged 34.3 kg/d (range 14.2–52.1 kg/d), milk fat averaged 3.47% (range 2.20–4.60%), and rumen pH averaged 6.1 (range 5.3–7.0). Rumination observation systems were categorized into 6 groups, but there was little difference in average rumination time among systems. The total time spent ruminating averaged 444 min/d (range 151–638 d) and occurred in 13.8 bouts/d (range 7.8–17.4 bouts/d) that averaged 32.7 min (range 20.0–48.1 min). Bivariate regressions were modeled to include the random effect of study, and correlations were evaluated through the partial R² that excluded variation accounted for by the random effect. Rumination time was quadratically increased with increasing milk fat yield (partial R² = 0.27) and milk fat percent (partial R² = 0.17). Rumination was also increased with increasing milk yield, dry matter intake, and rumen pH, and was quadratically related to dietary neutral detergent fiber (NDF) and total-tract NDF digestibility (partial R² = 0.10–0.27). Similar relationships were observed for rumination per unit of dry matter and NDF intake. The best-fit multivariate model predicting total rumination time included milk yield, milk fat yield, and concentration and accounted for 37% of the variation. Total-tract digestibility was available for 217 treatment means; when included in the model, the partial R² increased to 0.41. Last, principal component analysis was conducted to explore the relationship among variables. The first 2 principal components in the broad analyses explained 36.7% of the 39 variables evaluated, which included rumination bouts and time spent ruminating. In conclusion, rumination time was related to milk fat across a large number of studies, although it explained only a limited amount of the variation in milk fat.     

Physical effectiveness of corn silage fractions stratified with the Penn State Particle Separator for lactating dairy cows

This study evaluated the physical effectiveness of whole-plant corn silage (CS) particles stratified with the Penn State Particle Separator, composed of 19- and 8-mm-diameter sieves and a pan, for lactating dairy cows. Eight Holstein cows (27.6 ± 2.8 kg/d of milk, 611 ± 74 kg body weight; 152 ± 83 d in milk) were assigned to two 4 × 4 Latin squares (22-d periods, 16-d adaptation), where one square was formed with rumen-cannulated cows. Three CS particle fractions were manually isolated using the 8- and 19-mm diameter sieves and re-ensiled in 200-L drums. The 4 experimental diets were (% dry matter): (1) CON (control): 17% forage neutral detergent fiber (NDF) from CS (basal roughage), 31.5% starch, and 31.9% NDF; (2) PSPan: 17% forage NDF from CS + 9% NDF from CS particles <8 mm, 25.9% starch, and 37.9% NDF; (3) PS8: 17% forage NDF from CS + 9% NDF from CS particles 8 to 19 mm, 25.5% starch, and 38.3% NDF; and (4) PS19: 17% forage NDF from CS + 9% NDF from CS particles >19 mm, 24.9% starch, and 38.8% NDF. Cows fed PS8 had greater dry matter intake and energy-corrected milk yield (22.4 and 26.9 kg/d, respectively) than cows fed CON (20.8 and 24.7 kg/d) and PS19 (21.2 and 24.8 kg/d), but no difference was detected between PSPan (21.6 and 25.8 kg/d) and other treatments. Milk fat concentration was greater for PS8 than CON, with intermediate values for PSPan and PS19. Milk fat yield was greater for cows fed PS8 than CON and PS19, and cows fed PSPan secreted more fat than CON cows but were not different from cows fed the other 2 diets. Cows fed CON had a lower meal frequency than cows fed PSPan, shorter meal and rumination times than PS8, and greater meal size and lower rates of rumination and chewing than the other 3 diets. Total chewing per unit of NDF was higher for PS8 than PSPan, although neither treatment differed from CON or PS19. Cows fed PS19 had higher refusal of feed particles >19 mm than cows fed CON and PSPan. The refusal of dietary NDF and undigested NDF in favor of starch were all greater for PS19 than on the other treatments. Cows fed PS19 had a greater proportion of the swallowed bolus and rumen digesta with particles >19 mm than the other 3 diets. Cows fed CON had the lowest ruminal pH and greatest lactate concentration relative to the other 3 diets. Plasma lipopolysaccharide was higher for cows fed CON and PSPan than for cows fed PS8 and PS19, and serum d-lactate tended to be lower on PSPan than for CON and PS8. In summary, the inclusion of CS fractions in a low-forage fiber diet (CON) reduced signs of ruminal acidosis. Compared with CS NDF <8 and >19 mm, CS NDF with 8- to 19-mm length promoted better rumen health and performance of dairy cows. These results highlight the importance of adjusting CS harvest and formulating dairy diets based on the proportion of particles retained between the 8- and 19-mm sieves.     

Novel use of the wild species Cephalaria joppensis for silage preparation and its nutritive value for feeding lactating dairy cows

This study presents a novel method for use of the wild plant species Cephalaria joppensis (CJ) as agricultural forage for ruminants. Domesticated CJ tends to have higher crop mass yield per hectare than a commercial wheat variety (W) but is similar in in vitro dry matter (DM) digestibility. This study was composed of 3 experiments. Experiment 1 aimed to measure effects of ensiling CJ versus W in packed polyethylene-wrapped bales. Three types of ensiled bales were produced for each plant: 1) direct-cut CJ versus W packed solely; 2) direct-cut CJ versus W mixed as sole roughage source together with dietary ingredient and packed in bales to create CJ total mixed ration (CJ-TMR) or W-TMR; 3) CJ silage versus W silage mixed as one-third of dietary roughage source together with two-thirds sorghum (S) silage and additional dietary ingredients and packed in bales to create CJ-S-TMR or W-S-TMR. Data showed that packing and wrapping created anaerobic conditions within the 4 types of TMR bales while reducing pH (4.12 to 4.37). Dry matter loss during ensilage was higher for the 2 types of TMR containing W compared with CJ. Ensilage decreased soluble nitrate content as well as yeast and mold contamination, and the 4 types of TMR bales were characterized by a long outdoor shelf life (3 mo) and high stability under aerobic exposure. Experiment 2 aimed to measure the intake and digestibility by sheep of the 4 types of packed TMR after 90 d of ensiling. Data demonstrated higher voluntary intake of the CJ-TMR compared with the other TMR types. The CJ-TMR was characterized by higher digestibility of DM, crude protein, and neutral detergent fiber components compared with the CJ-S-TMR. Experiment 3 examined intake, digestibility, and milk production by 21 pairs of lactating cows individually fed CJ-S-TMR versus W-S-TMR. Similar intake (21.6 to 22.0 kg/d) and digestibility of DM and crude protein were observed in cows fed the 2 TMR types (68 to 69% and 66 to 68%, respectively). However, neutral detergent fiber and cellulose digestibility were slightly higher in the cows fed W-S-TMR and this was reflected in a small increase in their milk and energy-corrected milk yield (36.5 and 31.4 kg/cow per day, respectively) compared with cows fed CJ-S-TMR (35.5 and 30.4 kg/cow per day, respectively). Results demonstrate that direct-cut CJ used as is, or CJ silage can be included and ensiled in TMR bales for feeding productive ruminants as a substitute for wheat silage.     

Influence of corn silage hybrid type on lactation performance by Holstein dairy cows

The primary objective of this study was to determine lactation performance by dairy cows fed nutridense (ND), dual-purpose (DP), or brown midrib (BM) corn silage hybrids at the same concentration in the diets. A secondary objective was to determine lactation performance by dairy cows fed NutriDense corn silage at a higher concentration in the diet. One hundred twenty-eight Holstein and Holstein × Jersey cows (105 ± 38 d in milk) were stratified by breed and parity and randomly assigned to 16 pens of 8 cows each. Pens were then randomly assigned to 1 of 4 treatments. Three treatment total mixed rations (TMR; DP40, BM40, and ND40) contained 40% of dry matter (DM) from the respective corn silage hybrid and 20% of DM from alfalfa silage. The fourth treatment TMR had ND corn silage as the sole forage at 65% of DM (ND65). A 2-wk covariate adjustment period preceded the treatment period, with all pens receiving a TMR with equal proportions of DP40, BM40, and ND40. Following the covariate period, cows were fed their assigned treatment diets for 11 wk. nutridense corn silage had greater starch and lower neutral detergent fiber (NDF) content than DP or BM, resulting in ND40 having greater energy content (73.2% of total digestible nutrients, TDN) than DP40 or BM40 (71.9 and 71.4% TDN, respectively). Cows fed BM40 had greater milk yield than DP40, whereas ND40 tended to have greater milk yield and had greater protein and lactose yields compared with DP40. No differences in intake, component-corrected milk yields, or feed efficiency were detected between DP40, BM40, and ND40. Milk yield differences may be due to increased starch intake for ND40 and increased digestible NDF intake for BM40 compared with DP40. Intake and milk yield and composition were similar for ND40 compared with BM40, possibly due to counteracting effects of higher starch intake for ND40 and higher digestible NDF intake for BM40. Feeding ND65 reduced intake, and thus milk and component yields, compared with ND40 due to either increased ruminal starch digestibility or increased rumen fill for ND65. Nutridense corn silage was a viable alternative to both DP and BM at 40% of diet DM; however, lactation performance was reduced when nutridense corn silage was fed at 65% of DM.