进化免疫球蛋白结合分子LD5的原核表达、纯化及结合活性

目的对新型进化免疫球蛋白(Ig)结合分子LD5进行原核表达和纯化,并鉴定其结合活性。方法将LD5基因片段克隆入原核表达载体pET32a(+),转化大肠杆菌后,IPTG诱导表达。以8mol/L尿素裂解菌体后,上清经Ni2+-NTA柱纯化,并对纯化蛋白进行SDS-PAGE及Western blot分析。以ELISA和Dot blot对LD5蛋白结合IgG、IgM活性进行鉴定。结果LD5分子在大肠杆菌中获得成功表达,表达的融合蛋白相对分子质量为59000。Western blot表明纯化的LD5蛋白能特异结合IgG。ELISA结果显示,LD5在结合IgM上较SpA具有明显的优势。结论LD5分子在大肠杆菌中已成功表达,纯化的LD5蛋白保留了天然Ig结合分子的结合活性。     

分子印迹及结合技术去除水中污染物的研究进展

分子印迹技术具有高选择性、亲和性及稳定性等特点,在多污染物共存的体系中,为优先选择去除目标污染物提供了一条有效途径.本文介绍了分子印迹技术基本原理、分子印迹技术在水污染控制方面的研究进展,并根据已有的研究成果,提出了分子印迹技术在水处理中的发展方向和研究趋势,主要包括该技术去除污染物的动力学、热力学及去除机制研究、制备技术的优化、与其他技术的结合、应用于实际废水处理工程等.     

蛋白质分子印迹的应用研究进展

分子印迹技术由于其优异性能而被应用于多个领域中,在大多数情况下,其目标对象为小分子,对于生物大分子的印迹研究仍属于少数,但已取得了初步的进展,证明了分子印迹技术在生物大分子的检测和富集方面具有良好发展态势.本文主要总结了分子印迹在蛋白质印迹中的研究进展,分别介绍分子印迹对藻蓝蛋白、人血清白蛋白和肿瘤细胞标志蛋白的应用,...     

分子印迹技术最新研究进展

随着分子印迹技术的不断发展,越来越多的新型分子印迹聚合物出现在人们的视野中。重点介绍了几种新型分子印迹聚合物,并且展望了分子印迹技术的未来。     

海洋细菌代谢产物及应用研究进展

由于海洋环境特殊,海洋生物产生了与陆地生物不同的代谢系统和防御体系,分泌出多种结构新颖、活性特异的次级代谢产物,并且许多类型在陆地生物中难以发现。国内外研究人员已从海洋细菌中分离得到酶、铁载体、毒素、大环内酯、多糖和糖苷等多种产物,其中不少具有显著的抗细菌、抗病毒、抗真菌等活性,有望开发成新型药物或保健品。概述了海洋细菌代谢产物的主要类型及产生菌,并对其潜在应用做了简单介绍。     

细菌内毒素检查法在肺炎球菌结合疫苗研制中的应用及验证

目的应用细菌内毒素检查法(动态浊度法)检测13价肺炎球菌结合疫苗中细菌内毒素含量,并对该方法进行验证。方法用《中国药典》三部(2010版)载录的动态浊度法检查疫苗原辅材料、疫苗过程产物及疫苗细菌内毒素含量,并进行肺炎球菌结合疫苗原液和成品的细菌内毒素含量检测,验证方法的可行性及准确性。结果 13价肺炎球菌结合疫苗生产使用试剂、相关原辅材料和磷酸铝佐剂对细菌内毒素检查无影响;该方法线性、专属性、准确性、精密度、耐用性良好,检定范围初步定为0.08~10 EU/ml,定量限度暂定为0.03 EU/ml;可准确测定疫苗原液和成品中细菌内毒素含量,重复性和准确性良好。结论动态浊度法检测13价肺炎球菌结合疫苗中细菌内毒素含量,结果准确、稳定,符合方法验证的要求,可为该疫苗的质量控制提供稳定可靠的检测方法。     

卵黄免疫球蛋白IgY的研究及应用

随着生物化学和免疫学检测技术的飞速发展，实验用抗体的制备和纯化已成为一个非常重要的问题。目前在各种检测实验中，主要是应用哺乳动物的血清抗体，由于哺乳动物抗体本身的一些特点，使其对实验产生内源性干扰，影响检测的敏感性［1］。Williams等1962年研究发现，当用某种抗原免疫产蛋母鸡时，鸡可产生相应的血清抗体并通过产蛋的方式将抗体垂直遗传给后代而储存于蛋黄中［2］。由于卵黄中仅有IgG类抗体，故称其为卵黄免疫球蛋白lgG（YolKIgG简称为IgY）［3］。至今尚未证实蛋黄中有IgA和lgM的存在，只有少量存在于蛋清中［4］。…     

细菌Ⅴ型分泌系统的研究进展

革兰阴性(Gram-negative,G-)菌的分泌系统至少有9种,依次命名为Ⅰ～Ⅸ型分泌系统,其中V型分泌系统(type V secretion system,T5SS)亦被称为自主转运分泌系统(autotransporters,AT),广泛存在于G-菌中.该系统分泌的蛋白占G-菌外泌蛋白的比例最高.该系统只含1或2...     

静注人免疫球蛋白在治疗阿尔茨海默病中的应用

阿尔茨海默病(Alzheimer disease,AD)是最常见的神经退行性疾病。AD已经造成了严重的社会负担,而且目前仍无有效的方法可以治疗。近年来,使用静注人免疫球蛋白(intravenous immunoglobulin,IVIg)治疗AD被认为是一种有潜力的疗法。本文对AD、Aβ级联假说、IVIg治疗AD的作用机制以及临床试验等作一综述,并对未来IVIg治疗AD的研究进行了展望。     

分子印迹技术的原理及应用研究进展

分子印迹技术是20世纪70年代发展起来的一门特异性识别技术,进入21世纪以来在分离提纯、临床医学、痕量检测、酶催化等方面展现了不俗的表现,简要介绍分子印迹技术的基本原理、分类、特性以及应用。     

Reduced models of proteins and their applications

Reduced computer modeling of proteins now has a history of about 30 years. In spite of the enormous increase in computing abilities, reduced models are still very important tools for theoretical studies of protein structure, dynamics and thermodynamics. Very simple, highly idealized lattice (and recently also off-lattice) models could be studied in great detail, providing valuable insight into the most general factors governing structure stability, folding kinetics and interactions responsible for characteristic two-state behavior near the folding temperature. More complex models now enable modeling of real proteins on the level of low to moderate resolution, allowing us to address more detailed questions. Ab initio protein structure predictions, still being far from a routine task, have become feasible. When supported by evolutionary information from multiple sequence alignments and potential local and/or global structural similarity to known structures, reduced modeling opens up new areas of comparative modeling, thereby complementing contemporary structural genomics.     

Wollastonite raw materials and their applications (a review)

Natural and artificial wollastonite is used in the production of ceramics, devitrified glass (sitall), and as a polymer filler. Wollastonite additives decrease the firing temperature of ceramics, improve their strength, decrease the firing and drying shrinkages and the temperature of formation of the vitreous phase. Due to the high dielectric parameters, wollastonite ceramics are used in electrical and radio engineering. The use of wollastonite in the ceramics industry has good prospects.Translated from Steklo i Keramika, No. 9, pp. 13–16, September, 1995.     

Multiphasic calcium orthophosphate (CaPO4) bioceramics and their biomedical applications

Due to the chemical similarity to the inorganic constituents of calcified tissues of mammals, biologically relevant calcium orthophosphates (CaPO₄) have been applied as artificial bioceramics suitable for reconstruction of various types of bone defects. Since none of the known individual types of CaPO₄ appears to be able to mimic both the composition and the properties of natural bones, various attempts have been sought to overcome this problem and a multiphasic (polyphasic) concept is one of the reasonable solutions. In general, this approach is determined by advantageous formulations consisting of homogeneous blends of two (biphasic), three (triphasic) or more (multiphasic) individual CaPO₄ phases possessing diverse solubility and, therefore, bioresorbability, while the optimum ratios among the phases depend on the definite applications. Therefore, all currently known multiphasic CaPO₄ formulations are sparingly soluble in water and, thus, after being implanted they are gradually resorbed inside the body, releasing calcium and orthophosphate ions into the biological medium and, hence, seeding a new bone formation. They have already demonstrated a proven biocompatibility, osteoconductivity, safety and predictability in vitro, in vivo, as well as in clinical trials. More recently, in vitro and in vivo studies have shown that some of them might possess osteoinductive properties. Hence, in tissue engineering, multiphasic CaPO₄ bioceramics represent promising formulations to construct various scaffolds capable of carrying and/or modulating the behavior of cells. This review summarizes the available information on biphasic, triphasic and multiphasic CaPO₄ bioceramics including their biomedical applications. New formulations have been proposed as well.     

New modifications of poly(vinyl alcohol)s and their applications

Poly(vinyl alcohol) (PV-OH), prepared from poly(viny) acetate), is used widely in many industries. Various grades have been produced, with different degree of polymerization and degree of hydrolysis. Recently, novel modified (PV-OH)s with anion, cation, silanol or hydrophobic groups have been studied and developed. They have new properties in addition to those of ordinary PV-OH and have new applications. The methods of modification and the characteristics and some applications of the modified polymers are described.     

IgG-Fc融合蛋白的研究进展

IgG-Fc融合蛋白是将生物活性蛋白与IgG的铰链区和Fc片段进行基因重组而表达的融合蛋白。IgG-Fc融合蛋白不仅可发挥所融合蛋白的生物学活性,还赋予其类似抗体的特性,包括延长血浆半衰期以及Fc片段特有的一系列效应功能,并已广泛应用于临床治疗、生物医学研究等领域。合理优化IgG-Fc融合蛋白是未来发展趋势,选择合适的IgG亚类作为融合载体,采用Fc片段单个或多个氨基酸序列修饰、糖基化改造等,提高融合蛋白的血浆半衰期、优化融合蛋白的效应功能,最终达到最佳免疫治疗效果。本文对IgG-Fc融合蛋白的功能、相应优化策略作一综述,并介绍了其临床研究现状。     

聚离子液体及其在天然产物分离中的应用

天然产物资源丰富,其高效利用离不开先进的分离技术。兼具离子液体高选择性及聚合物安全、易回收等特性的聚离子液体为天然产物的分离提供了新途径。在介绍聚阳离子型、聚阴离子型和聚两性型离子液体制备技术的基础上,综述了使用聚离子液体的固相萃取、固相微萃取、分子印迹固相萃取、液液萃取及毛细管电泳技术在黄酮类、生物碱类、酚类、蛋白质类等天然产物分离中的研究进展,分析了聚离子液体分离作用机理及其具有良好的分离效率、稳定性和回收再利用性能的原因,特别强调了刺激响应聚离子液体在天然产物分离中的优势,并从聚离子液体设计和定制出发对聚离子液体在分离领域所存在的挑战和潜在的应用进行了展望。     

碱金属醇盐和碱金属酚盐的合成及其在橡胶中的应用

综述了碱金属醇、酚盐几种不同的合成方法及其在液体聚丁二烯橡胶、丁苯橡胶和有机合成等领域的应用。     

Rare earth (RE) doped phosphors and their emerging applications: A review

The evolution of luminescent materials has witnessed rapid advancement in research and development. Solid inorganic light-emitting materials or phosphors are the optoelectronic material of the 21st century because of their power-efficient potential over various illumination sources, eco-friendliness and resourceful display perspectives. The inorganic phosphors have been extensively explored to meet the demand of low voltage stimulated lighting sources owing to increased global energy consumption. Due to environmental friendliness, advantages long lifetime, lower energy consumption, reliability and high luminous efficiency, modern white light-emitting diodes (WLEDs) have replaced less effective incandescent and mercury-enclosing conventional fluorescent lighting sources. This review highlights the developments in preparation, luminescence and potential perceptions of rare-earth activated phosphors for solid-state lighting technologies. The role of RE ions as an activator as well as a sensitizer in doped materials and possible transitions within their energy levels are reviewed in detail. The paper reviews the substantial influence of host lattices such as aluminate, oxide, phosphate, silicate, sulfide, etc on the optical transitions of doped RE ions. Studies on the advancement into the design of novel phosphors are very crucial as they will provide an opportunity to boom prospects in the course of promising applications. The sustainable energy facilities include clean technologies providing a cheaper lighting source which can produce significant indirect economic benefits via limiting the deforestation and use of scrubbing technology to mitigate air pollution.     

Preparation and characterization of immobilized ion exchange polyurethanes (IEPU) and their applications for continuous electrodeionization (CEDI)

Immobilized ion-exchange polyurethanes (IEPU) were prepared as ion conducting spacers in continuous electrodeionization (CEDI) for the treatment of a synthetic primary coolant. Ion exchange resins were immobilized by using allophanate/biuret cross-linking in preparation of polyurethane. Synthesized IEPU was characterized in terms of mechanical strength, ion exchange capacity (TEC), electrical conductivity and the porous plug model, which schematically represents the transport pattern through the IEPU. CEDI was carried out in a laboratory scale with an effective area of 20 cm². The CEDI operation with a layered bed configuration showed the main removal mechanism of cobalt ion was dependent on the active surface area between ion conducting materials. The performance of the CEDI operation showed over 98% removal of cobalt ions, suggesting the feasibility of IEPU as ion conducting spacers in a CEDI system.