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1.
Inhibitors of enzymatic amplification in serum may cause false-negative results for direct detection of hepatitis C virus (HCV) by polymerase chain reaction (PCR). This study was undertaken to demonstrate the importance of the internal control in a PCR assay for detection of HCV-RNA to monitor false-negatives due to inhibitors. HCV-RNA was extracted using RNA extraction kit (SepaGene RV-R, Sanko Junyaku) and a prototype instrument for automated specific capture of HCV-RNA with probes and magnetic bead/fluid separation (Roche Molecular Systems). The extracted HCV-RNA and internal control were detected by an automated PCR machine (Cobas Amplicor, Roche Diagnostic Systems). Addition of hemoglobin (up to 4.5 g/l) to the sera followed by RNA extraction with SepaGene RV-R had no inhibitory effect on the detection of either HCV-RNA or the internal control. In contrast, addition of heparin to the sera showed an inhibitory effect with a dose-dependent manner on the detection of both HCV-RNA and the internal control, with a greater effect at lower copy number of HCV. When HCV-RNA was extracted by the automated system, the inhibitory effect of heparin was successfully eliminated. In the assays of 65 serum samples positive for anti-HCV antibodies, positivity for the internal control indicated efficient amplification and validated 14 negative and two equivocal results for detection of HCV-RNA. Detection of the internal control was negatively correlated with viral copy number in sera suggesting competitive inhibition of high viral copy number on amplification of the internal control. Extraction, co-amplification and detection of the internal control appears useful for estimating effects of inhibitors on amplification in each assay for the detection of HCV-RNA, and for evaluating efficacy of RNA extraction methods.  相似文献   

2.
Presence of air in acute extradural hematomas has been described by several authors. Little attention has been paid to its significance. We report six pediatric patients with air-containing extradural hematomas. A fracture involving air-containing structures in either anterior or middle cranial fossa was detected on computed tomographic scans of all patients. The pertinent literature was reviewed.  相似文献   

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The meniscal impingement syndrome consists of three elements: impaction on the anterior medial femoral condyle by the leading edge of the medial meniscus, articular cartilage damage of at least Outerbridge grade 3, and knee hyperextension of at least 5 degrees. This report reviews this condition in a series of seven knees with an average follow-up of 39 months. The time from the onset of symptoms until surgery averaged 45 months. Treatment consisted of a thorough arthroscopic knee evaluation and debridement of the articular cartilage fragmentation and any impinging synovitis. Postoperative rehabilitation includes extension block bracing, hamstring strengthening, and closed-chain exercise. With this regimen, there was improvement in the Tegner scores and a reduction in postoperative knee hyperextension. Identification of this uncommon condition requires a complete evaluation of the medial femoral condyle in patients with knee hyperextension.  相似文献   

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A woman who was treated for intrinsic urethral sphincteric deficiency with periurethral injection of glutaraldehyde cross-linked collagen had prolapse of the urethral mucosa and recurrence of incontinence. She subsequently required surgical resection and a fascia lata sling. This is the first known occurrence of this postinjection complication.  相似文献   

7.
Autologous fat injection has been widely used during the past 20 years to correct contour anomalies. We have been using suction-assisted lipectomy for the past 13 years, and we have used the aspirated fat to correct contour irregularities on several occasions. We present the case of a 44-year-old woman who, after autologous fat transplantation to correct a contour anomaly (depression secondary to a previous operation) at her left groin, developed a large cyst containing pearl-like fat lobules. The cyst was first noticed 3 months postoperatively and was removed under local anesthesia 5 months postoperatively. Histology confirmed that the lobules were mature fat cells.  相似文献   

8.
Shoulder dystocia is an unpredictable and potentially serious obstetric emergency. By applying a recognized and rehearsed series of manoeuvres, maternal and neonatal morbidity can be minimized.  相似文献   

9.
The most common source of shoulder pain is soft-tissue dysfunction. Although fractures of the humerus, scapula, and clavicle are common throughout life, most problems encountered between the ages of 15 and 60 are related to the ligaments, tendons, and muscles of the shoulder girdle. This article deals mainly with tendinitis and tendinosis as primary entities, and to a lesser extent with tendon problems attributable to a primarily bony or ligamentous dysfunction.  相似文献   

10.
The importance of the labrum and glenohumeral ligament complex for the stability of the shoulder joint has been known since the beginning of this century. Shoulder instability may be classified into two large groups. TUBS, characterized by Traumatic, Unidirectional instability and Bankart lesion which often requires Surgery. The second group AMBRI, is characterized by Atraumatic, Multidirectional, Bilateral instability that often responds to Rehabilitation, but in case of surgery Inferior capsular shift is indicated. The high recurrence rate after primary traumatic anterior dislocation in young adults has caused an ongoing discussion about the indications for primary surgery. Anatomical reconstruction focusing on the pathoanatomy is recommended due to better results and a lower complication rate as compared to non-anatomical reconstruction that alters the normal anatomy and kinematics. The recurrence rate after arthroscopic Bankart repair is still generally higher than after open surgery but may be improved by better patient selection.  相似文献   

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Because xenon NMR is highly sensitive to the local environment, laser-polarized xenon could be a unique probe of living tissues. Realization of clinical and medical science applications beyond lung airspace imaging requires methods of efficient delivery of laser-polarized xenon to tissues, because of the short spin-lattice relaxation times and relatively low concentrations of xenon attainable in the body. Preliminary results from the application of a polarized xenon injection technique for in vivo 129Xe NMR/MRI are extrapolated along with a simple model of xenon transit to show that the peak local concentration of polarized xenon delivered to tissues by injection may exceed that delivered by respiration by severalfold.  相似文献   

13.
The significance of the bolus injection test lies in supplying data for evaluation of the cerebrospinal fluid (CSF) bulk flow. It provides pressure-time (PT) curves by the process of direct recording, while the pressure-volume (PV) and volume-time (VT) plots are subsequently computed...  相似文献   

14.
Currently a myriad of devices are available for immobilization of the injured or postsurgical upper extremity. Some of these devices are straightforward and easily used, but some are more complicated and require more familiarity for their successful application. However, even simple devices have the potential for misapplication and thus prevent their benefit to the patient. This article is the third in a 3-part series. The goals of the series are (1) to present and review several devices on the market used by shoulder surgeons to immobilize the upper extremity, and (2) to discuss proper application and precautions of their use. It is intended that this series will benefit nurses, therapists, and trainers involved in the use of these devices.  相似文献   

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对同轴射流的套筒喷枪和交叉射流的斜插喷枪的流动特性进行了分析和对比。在COREX喷煤模拟燃烧炉内,对两种喷枪进行了喷煤试验。通过比较不同参数条件下喷吹煤粉的利用率,发现套筒式喷枪的效果要优于斜插冷却喷煤管喷枪。其原因是相同流速和流量条件下,同轴射流比交叉射流的行程要长,即煤粉与氧接触的时间长,因此套筒式喷枪有利于煤粉燃烧。  相似文献   

17.
We report on anaphylactoid reactions (urticae at the injection site and along the injection vein) after intravenous injections of pethidine in two patients. Skin tests with pethidine were negative, but intravenous challenge showed urticae. These results indicate that the reactions were due to a non-immunological mechanism resembling pharmacological intolerance. Retrospective analysis revealed a 5.6% incidence of pharmacological intolerance reactions to intravenous pethidine in 519 patients.  相似文献   

18.
We report a unique case of attempted suicide by intramuscular injection of the organophosphate isofenphos which resulted in a muscarinic and nicotinic syndrome lasting 15 days and requiring prolonged mechanical ventilation and hospitalization. The patient, who demonstrated no signs of delayed polyneuropathy on hospital day 25, subsequently died of pneumonia. Toxicological investigations showed isofenphos plasma decay and confirmed the intramuscular route of poisoning. We believe continuous isofenphos absorption resulted in the prolonged intoxication observed in this patient.  相似文献   

19.
In the current decade impingement syndrome is becoming a less precise diagnostic entity. It is splitting into several categories of more exact diagnoses. The concept development, clinical picture, and currently recommended treatment of 1 of these entities, the superior glenoid impingement is reviewed. The complaint may be acute or chronic and may involve 1 or more of 5 structures: (1) superior labrum, (2) rotator cuff tendon, (3) inferior glenohumeral ligament, (4) greater tuberosity, and (5) the bony glenoid. The most commonly seen clinical entity is chronic dorsal shoulder pain in an athlete who throws with a positive relocation test. Treatment consists of strengthening of the cuff and scapular rotators. When there has been excessive inferior ligament stretch this must be augmented by anterior reconstruction.  相似文献   

20.
Ankle arthroscopy has recently allowed the elaboration of less invasive techniques for the treatment of anterior impingement. Its indications, advantages, and drawbacks in this application are discussed. Between 1987 and 1994, 133 patients were treated for ankle impingement. Among them, 58 patients, 37 men and 21 women (mean age, 28.5 years), who had failed a trial of conservative treatment were treated by means of tibiotalar arthroscopy. Twenty-seven were athletes engaged in sports with abnormal stressing of the ankle. According to McDermott's radiological classification, there were 15 stage I cases, 23 stage II, 13 stage III, and 7 stage IV. Preoperative evaluation with a modified version of McGuire's scoring system gave 50 cases rated as "poor" (< 60 points) and 8 cases rated as "fair" (60-67 points). Treatment consisted of removal of adhesions, cartilage shaving, and removal of the bone impingement with powered instruments, curettes, or small osteotomes. Follow-up was from 8 to 62 months (mean, 21.5 months). The postoperative McGuire ratings were 37 good, 13 fair, and 8 poor. There were no major complications. Recurrence of impingement was observed in four cases of stage III and IV. The conclusion is drawn that ankle arthroscopy is a sound method for the treatment of anterior impingement. Even in cases with severe joint cartilage impairment, it plays a therapeutic role as a means of postponing a possible arthrodesis.  相似文献   

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