共查询到20条相似文献,搜索用时 15 毫秒
1.
Nan-Qi Ren Bing-Feng Liu Guo-Xiang Zheng De-Feng Xing Xin Zhao Wan-Qian Guo Jie Ding 《International Journal of Hydrogen Energy》2009,34(18):7579-7584
In order to obtain the high H2 yield, photo-hydrogen production by Rhodopseudomonas faecalis strain RLD-53 in fed-batch culture using acetate as the sole carbon was studied. In repeated fed-batch culture, biomass increased rapidly from 0.05 to 0.65 g/l within 120 h, with the specific maximal growth rate estimated 0.68 × 10−3 g/h. After 120 h, biomass increased slowly and after each time feeding biomass increased slightly. The specific cumulative H2 volumes in each phase were 2791.3, 1161.7, 1445.5 and 840.6 ml H2/l-culture, respectively. The average H2 yield was 3.17 mol H2/mol acetate based on the whole process while the average substrate conversion efficiency reached 79.3%. Specific maximum H2 production rate and H2 content was 37.2 ml H2/l/h and 95.5%, respectively. The results demonstrated the repeated fed-batch mode obtained higher efficiency for hydrogen production, feeding acetate concentration and control of pH were important to fed-batch culture hydrogen production. 相似文献
2.
Interest in renewable and clean energies such as hydrogen has increased because of the high level of polluting emissions, increasing costs associated with petroleum and the escalating problems of global climate change. In the presence of a light source, a microbial photosynthetic process provides a system for the conversion of some organic compounds into biomass and hydrogen. Using Rhodopseudomonas palustris as a cell-factory, hydrogen photo-evolution was investigated in a photobioreactor (PBR) irradiated either from one or two opposite sides. Irradiating the photobioreactor from only one side, in the presence of malic acid, a reactor hydrogen production of 2.786 l(H2) PBR−1 was achieved. When the PBR was irradiated from two opposite sides, hydrogen photo-evolution increased to 3.162 l(H2) PBR−1. Experiments were carried out using inoculum from either the retardation or the exponential growth phases. Using the latter, the highest hydrogen photo-evolution rate based on the bacteriochlorophyll (Bchl) concentration was achieved (3295 μl(H2) mg (Bchl−1 h−1). The hydrogen to biomass ratio (rg) was 1.91 l g−1 in the medium containing malic acid and 1.07 l g−1 in that containing acetic acid. It was found that the hydrogen production rate was higher with malic than with acetic acid. Although photobiological hydrogen production cannot furnish alone the greater and greater world requirements of clean renewable energy, it is desirable that photobiological hydrogen technology will grow, in the near future, because photobioreactors for bio-hydrogen production can be positioned in fringe areas without competition with agricultural lands. 相似文献
3.
Wenming Zong Ruisong Yu Peng Zhang Meizhen Fan Zhihua Zhou 《Biomass & bioenergy》2009,33(10):1458-1463
A two-step process of sequential anaerobic (dark) and photo-heterotrophic fermentation was employed to produce hydrogen from cassava and food waste. In dark fermentation, the average yield of hydrogen was approximately 199 ml H2 g−1 cassava and 220 ml H2 g−1 food waste. In subsequent photo-fermentation, the average yield of hydrogen from the effluent of dark fermentation was approximately 611 ml H2 g−1 cassava and 451 ml H2 g−1 food waste. The total hydrogen yield in the two-step process was estimated as 810 ml H2 g−1 cassava and 671 ml H2 g−1 food waste. Meanwhile, the COD decreased greatly with a removal efficiency of 84.3% in cassava batch and 80.2% in food waste batch. These results demonstrate that cassava and food waste could be ideal substrates for bio-hydrogen production. And a two-step process combining dark fermentation and photo-fermentation was highly improving both bio-hydrogen production and removal of substrates and fatty acids. 相似文献
4.
Maneewan Suwansaard Wanna Choorit Jill H. Zeilstra-Ryalls Poonsuk Prasertsan 《International Journal of Hydrogen Energy》2009,34(17):7523
We are developing a process to produce biohydrogen from palm oil mill effluent. Part of this process will involve photohydrogen production from volatile fatty acids under low light conditions. We sought to isolate suitable bacteria for this purpose from Songkhla Lake in Southern Thailand. Enrichment for phototrophic bacteria from 34 samples was conducted providing acetate as a major carbon source and applying culturing conditions of anaerobic-low light (3000 lux) at 30 °C. Among the independent isolates from these enrichments 19 evolved hydrogen with productivities between 4 and 326 ml l−1 d−1. Isolate TN1 was the most efficient producer at a rate of 1.85 mol H2 mol acetate−1 with a light conversion efficiency of 1.07%. The maximum hydrogen production rate for TN1 was determined to be 43 ml l−1 h−1. Environmentally desirable features of photohydrogen production by TN1 included the absence of pH change in the cultures and no detectable residual CO2. 相似文献
5.
Lívian Ribeiro Vasconcelos de Sá Magali Christe Cammarota Tatiane Corrêa de Oliveira Edna Maria Morais Oliveira Andrea Matos Viridiana Santana Ferreira-Leitão 《International Journal of Hydrogen Energy》2013
Biofuels production in Brazil is a traditional activity, and it has becoming more important each year. Within this context, biohydrogen production could exploit residual streams from first generation ethanol (ethanol 1G), second generation ethanol (ethanol 2G) and biodiesel production. Therefore hexoses, pentoses and glycerin were tested as substrates for hydrogen production. Firstly, the effects of different inoculum pretreatments (acid, alkaline and heat) on bacterial communities' performance were evaluated through the levels of Clostridium hydrogenase expression. The heat pretreated inoculum provided the highest yield of H2 (4.62 mol H2/mol sucrose) and also the highest level of hydrogenase expression, 64 times higher when compared with untreated inoculum after 72 h. Then C5 and C6 sugars and also glycerin were tested for H2 production (35 °C and pH 5.5), which resulted in promising yields of H2: sucrose (4.24 mol H2/mol sucrose), glucose (2.19 mol H2/mol glucose), fructose (2.09 mol H2/mol fructose), xylose (1.88 mol H2/mol xylose) and glycerin (0.80 mol H2/mol glycerin). 相似文献
6.
Since hydrogen is a renewable energy source, biohydrogen has been researched in recent years. However, there is little data on hydrogen fermentation by a leachate from a waste landfill as inoculum. We investigated hydrogen production using a leachate from an industrial waste landfill in Kanagawa prefecture. The results showed no methane gas production and the leachate was a suitable inoculum for hydrogen fermentation. The maximum H2 yield was 2.67 mol of H2 per mol of carbohydrate added, obtained at 30 °C and initial pH 7. The acetate and butyrate production was significant when the H2 yield was higher. The oxidation–reduction potential analysis of the culture suggested that hydrogen-producing bacteria in the leachate were facultatively anaerobic. Scanning electron microscope observations revealed hydrogen-producing bacteria comprised bacilli of about 2 μm in length. 相似文献
7.
Yuanyuan Wang Yanlin Zhang Liang Meng Jianbo Wang Wenqian Zhang 《Biomass & bioenergy》2009,33(9):1131-1138
A two-phase anaerobic process to produce hydrogen and methane from swine manure was investigated, using pretreated sludge with heat, acid and alkali treatment as inoculum. The relative order of pretreatment methods of H2 productivity effectiveness and CH4 productivity effectiveness produced by the residua of the first phase was heat treatment > alkali treatment > acid treatment. When the inoculum sludge was heat-treated at 80°C for 30 min, the H2 and CH4 production rate was the highest of 36.6, 201.7 ml (g TS)added−1. There were significant correlations between biogas production and accumulation of acetic acid and butyric acids. When propionic acid and total VFA concentrations reached about 2850 mg L−1 and 10.0 g L−1, respectively, the average H2 production rate and H2 content decreased from 7.6 ml d−1(g VS)added−1 and 55.3% to 1.4 ml d−1(g VS)added−1 and 43.2%, respectively. The activity of methanogenic bacteria was inhibited to a significant extent when the total VFA concentration was above 10.0 g L−1, but this inhibitory effect weakened when the VFA concentration fell to 6200–8500 mg L−1. Correspondingly, average CH4 production rate increased from 4.0 ml d−1(g TS)added−1 to 12.5 ml d−1(g TS)added−1. Propionic acid was degraded rapidly only when acetic and butyric acid concentrations dropped to 2500 mg L−1 and 1000 mg L−1, respectively. 相似文献
8.
P. Bakonyi N. Nemestóthy É. Lövitusz K. Bélafi-Bakó 《International Journal of Hydrogen Energy》2011,36(21):13949-13954
Escherichia coli is attractive for biotechnological hydrogen production. Compared to other biohydrogen producing bacteria e.g. Clostridium species, E. coli is able to tolerate oxygen, fast growing and well-characterized in physiological and biochemical terms. According to the well known metabolic pathways of E. coli, the hydrogen production from different substrates is dependent on the membrane-boundary formate-hydrogen lyase (FHL) enzyme complex. The efficiency and economic success of hydrogen fermentation are influenced by the applied operational conditions. In this work the optimal conditions (composition of broth, inoculum size, stirring speed) for biohydogen fermentation using E. coli (XL1-BLUE) were investigated by experimental design. We found that among the several variables only formate compound plays a key role in hydrogen formation and the optimal conditions for biohydrogen production were identified as follows: 30 mM formate, 5 g/l yeast extract, 10 g/l tryptone, 3.33 g/l NaCl, 0.05 g dry cell weight/l initial cell density and 220 rpm stirring rate, where productivity and yield were 426 ml H2 l−1 d−1 and 0.41 mol H2/mol formate, respectively. 相似文献
9.
Julien Masset Serge Hiligsmann Christopher Hamilton Laurent Beckers Fabrice Franck Philippe Thonart 《International Journal of Hydrogen Energy》2010
This paper reports investigations carried out to determine the optimum culture conditions for the production of hydrogen with a recently isolated strain Clostridium butyricum CWBI1009. The production rates and yields were investigated at 30 °C in a 2.3 L bioreactor operated in batch and sequenced-batch mode using glucose and starch as substrates. In order to study the precise effect of a stable pH on hydrogen production, and the metabolite pathway involved, cultures were conducted with pH controlled at different levels ranging from 4.7 to 7.3 (maximum range of 0.15 pH unit around the pH level). For glucose the maximum yield (1.7 mol H2 mol−1 glucose) was measured when the pH was maintained at 5.2. The acetate and butyrate yields were 0.35 mol acetate mol−1 glucose and 0.6 mol butyrate mol−1 glucose. For starch a maximum yield of 2.0 mol H2 mol−1 hexose, and a maximum production rate of 15 mol H2 mol−1 hexose h−1 were obtained at pH 5.6 when the acetate and butyrate yields were 0.47 mol acetate mol−1 hexose and 0.67 mol butyrate mol−1 hexose. 相似文献
10.
Inoculum pre-treatment is a crucial aspect of hydrogen fermentation processes to establish the required microbial community for hydrogen production. This paper models and optimizes two hybrid techniques of inoculum pre-treatment for fermentative hydrogen production: 1pH and Autoclave (PHA); 2pH and heat shock (PHS) using Response Surface Methodology (RSM). Coefficients of determination (R2) of 0.93 and 0.90 were obtained for PHA and PHS respectively and the optimized pre-treatment conditions gave hydrogen yields up to 1.35 mol H2/mol glucose and 0.75 mol H2/mol glucose, thus a 37.75% and 15.38% improvement on model predictions for PHA and PHS respectively. 相似文献
11.
Sandra I. Maintinguer Bruna S. Fernandes Iolanda C.S. Duarte Nora Kátia Saavedra M. Angela T. Adorno M. Bernadete A. Varesche 《International Journal of Hydrogen Energy》2011,36(21):13508-13517
Hydrogen production was obtained from low concentrations of xylose metabolized by heat treated inoculum obtained from the slaughterhouse wastewater treatment UASB reactor installed in Brazil. The molecular biological analysis Clostridium and Klebsiella species, recognized as H2 and volatile acid producers, in addition to Burkholderia species and uncultivated bacteria. The assays were carried out in batch reactors: (1) 630.0 mg xylose/L, (2) 1341.0 mg xylose/L, (3) 1848.0 mg xylose/L and (4) 3588.0 mg xylose/L. The following yields were obtained: 3% (0.2 mol H2/mol xylose), 8% (0.5 mol H2/mol xylose), 10% (0.6 mol H2/mol xylose) and 14% (0.8 mol H2/mol xylose), respectively. The end products obtained were acetic acid, butyric acid, methanol and ethanol in all of the anaerobic reactors. The concentrations of xylose did not inhibit microbial growth and hydrogen production. This suggested that low concentrations of xylose should be added to wastewater to produce hydrogen. 相似文献
12.
Qin Sun Wei Xiao Dan Xi Jiping Shi Xing Yan Zhihua Zhou 《International Journal of Hydrogen Energy》2010
Statistically based experimental designs were applied to optimize the fermentation process parameters for hydrogen (H2) production by co-culture of Clostridium acidisoli and Rhodobacter sphaeroides with sucrose as substrate. An initial screening using the Plackett–Burman design identified three factors that significantly influenced H2 yield: sucrose concentration, initial pH, and inoculum ratio. These factors were considered to have simultaneous and interdependent effects. A central composite design and response surface analysis were adopted to further investigate the mutual interactions among the factors and to identify the values that maximized H2 production. The optimal substrate concentration, initial pH, and inoculum ratio of C. acidisoli to R. sphaeroides were 11.43 g/L sucrose, 7.13, and 0.83, respectively. Using these optimal culture conditions, substrate conversion efficiency was determined as 10.16 mol H2/mol sucrose (5.08 mol H2/mol hexose), which was near the expected value of 10.70 mol H2/mol sucrose (5.35 mol H2/mol hexose). 相似文献
13.
Poonsuk Prasertsan Sompong O-Thong Nils-Kre Birkeland 《International Journal of Hydrogen Energy》2009,34(17):7448
The optimum values of hydraulic retention time (HRT) and organic loading rate (OLR) of an anaerobic sequencing batch reactor (ASBR) for biohydrogen production from palm oil mill effluent (POME) under thermophilic conditions (60 °C) were investigated in order to achieve the maximum process stability. Microbial community structure dynamics in the ASBR was studied by denaturing gradient gel electrophoresis (DGGE) aiming at improved insight into the hydrogen fermentation microorganisms. The optimum values of 2-d HRT with an OLR of 60 gCOD l−1 d−1 gave a maximum hydrogen yield of 0.27 l H2 g COD−1 with a volumetric hydrogen production rate of 9.1 l H2 l−1 d−1 (16.9 mmol l−1 h−1). The hydrogen content, total carbohydrate consumption, COD (chemical oxygen demand) removal and suspended solids removal were 55 ± 3.5%, 92 ± 3%, 57 ± 2.5% and 78 ± 2%, respectively. Acetic acid and butyric acid were the major soluble end-products. The microbial community structure was strongly dependent on the HRT and OLR. DGGE profiling illustrated that Thermoanaerobacterium spp., such as Thermoanaerobacterium thermosaccharolyticum and Thermoanaerobacterium bryantii, were dominant and probably played an important role in hydrogen production under the optimum conditions. The shift in the microbial community from a dominance of T. thermosaccharolyticum to a community where also Caloramator proteoclasticus constituted a major component occurred at suboptimal HRT (1 d) and OLR (80 gCOD l−1 d−1) conditions. The results showed that the hydrogen production performance was closely correlated with the bacterial community structure. This is the first report of a successful ASBR operation achieving a high hydrogen production rate from real wastewater (POME). 相似文献
14.
In present work our objective was to find out the potential substrates for fermentative hydrogen production using microbial culture of Bacillus firmus NMBL-03 isolated from municipal sludge. A wide variety of substrates (glucose, xylose, arabinose, lactose, sucrose, and starch) and carbohydrate rich waste products (bagasse hydrolysate, molasses, potato peel and cyanobacterial mass) have been used for dark fermentative hydrogen production. All studies were done at optimized physico-chemical conditions. Among all substrates glucose, arabinose, lactose, starch, molasses and bagasse hydrolysate were found to be the favorable substrates for hydrogen production. The highest VHPR i.e. 177.5 ± 7.07 ml/L.h (7.95 ± 0.29 mmol H2/L.h) and maximum H2 production (22.58 ± 2.65 mmol H2/L) was achieved using starch as the substrate. The maximum yield 1.29 ± 0.11 mol H2/mol reducing sugar was obtained from bagasse hydrolysate as substrate. Butyrate and acetate were detected as the end product in all the cases while lactate was also detected from glucose and cyanobacterial hydrolysate. Since considerable amount of H2 also evolved when cyanobacterial mass was used, therefore this microbe can be exploited for hydrogen production through a three stage integrated system. Residual carbohydrate containing biomass left after cyanobacterial H2 production can be utilized in dark fermentative H2 production. Spent media obtained after dark fermentative H2 production contain considerable amount of volatile fatty acids that can be potential substrate for photo fermentative H2 production. 相似文献
15.
Rathnasiri G. Puhulwella Laurent Beckers Frank Delvigne Alina S. Grigorescu Philippe Thonart Serge Hiligsmann 《International Journal of Hydrogen Energy》2014
This study investigates the mesophilic biohydrogen production from glucose using a strictly anaerobic strain, Clostridium butyricum CWBI1009, immobilized in a trickling bed sequenced batch reactor (TBSBR) packed with a Lantec HD Q-PAC® packing material (132 ft2/ft3 specific surface). The reactor was operated for 62 days. The main parameters measured here were hydrogen composition, hydrogen production rate and soluble metabolic products. pH, temperature, recirculation flow rate and inlet glucose concentration at 10 g/L were the controlled parameters. The maximum specific hydrogen production rate and the hydrogen yield found from this study were 146 mmol H2/L.d and 1.67 mol H2/mol glucose. The maximum hydrogen composition was 83%. Following a thermal treatment, the culture was active without adding fresh inoculum in the subsequent feeding and both the hydrogen yield and the hydrogen production rate were improved. For all sequences, the soluble metabolites were dominated by the presence of butyric and acetic acids compared to other volatile fatty acids. The results from the standard biohydrogen production (BHP) test which was conducted using samples from TBSBR as inoculum confirmed that the culture generated more biogas and hydrogen compared to the pure strain of C. butyricum CWBI1009. The effect of biofilm activity was studied by completely removing (100%) the mixed liquid and by adding fresh medium with glucose. For three subsequent sequences, similar results were recorded as in the previous sequences with 40% removal of spent medium. The TBSBR biofilm density varied from top to bottom in the packing bed and the highest biofilm density was found at the bottom plates. Moreover, no clogging was evidenced in this packing material, which is characterized by a relatively high specific surface area. Following a PCA test, contaminants of the Bacillus genus were isolated and a standard BHP test was conducted, resulting in no hydrogen production. 相似文献
16.
Sopee Panin Worajit Setthapun Alicia Amelia Elizabeth Sinsuw Hathaithip Sintuya Chen-Yeon Chu 《International Journal of Hydrogen Energy》2021,46(27):14073-14082
This study conducted the utilization of vegetable residues by an enriched microflora inoculum to produce biohydrogen via anaerobic batch reactor. Dark fermentation processes were carried out with 3 kinds of vegetable residue substrates including broccoli (Brassica oleracea var. italica.), onion (Alium cepa Linn.), and sweet potato (Ipomoea batatas (L.) Lam). Vegetable wastes were pretreated into 2 forms, i.e. mashed and powdered vegetable, prior to the fermentation. The substrate used for the biohydrogen production were vegetable residues and inoculum at the vegetable residues/inoculum ratio of 1:1 (based on TS). The digestion processes were performed under 120 rpm speed of shaking bottle in the incubator with control temperature of 35?C. In this work, the maximum hydrogen production was achieved by anaerobic digestion at mashed onion with bioreactor inoculum that produced total hydrogen of 424.1 mL H2 with hydrogen yield and hydrogen concentration of 151.67 mL H2/g VSadded and 43.54%, respectively. In addition, the hydrogen production continues took only 7 days for the vegetables blended with the bioreactor inoculum. Finally, it was found that the high potential of degradation of vegetable wastes an enriched microflora in dark fermentation also showed alternative solution to eliminate agricultural wastes to produce green energy. 相似文献
17.
E.P. Leaño S. Babel 《Renewable Energy》2012,39(1):339-346
Batch production of biohydrogen from cassava wastewater pretreated with (i) sonication, (ii) OPTIMASH BG® (enzyme), and (iii) α-amylase (enzyme) were investigated using anaerobic seed sludge subjected to heat pretreatment at 105 °C for 90 min. Hydrogen yield at pH 7.0 for cassava wastewater pretreated with sonication for 45 min using anaerobic seed sludge was 0.913 mol H2/g COD. Results from pretreatment with OPTIMASH BG® at 0.20% and pH 7 showed a hydrogen yield of 4.24 mol H2/g COD. Superior results were obtained when the wastewater was pretreated with α-amylase at 0.20% at pH 7 with a hydrogen yield of 5.02 mol H2/g COD. In all cases, no methane production was observed when using heat-treated sludge as seed inoculum. Percentage COD removal was found to be highest (60%) using α-amylase as pretreatment followed by OPTIMASH BG® at 54% and sonication (40% reduction rate). Results further suggested that cassava wastewater is one of the potential sources of renewable biomass to produce hydrogen. 相似文献
18.
Acid hydrolysis of corn stover for biohydrogen production using Thermoanaerobacterium thermosaccharolyticum W16 总被引:1,自引:0,他引:1
Guangli Cao Nanqi Ren Aijie Wang Duu-Jong Lee Wanqian Guo Bingfeng Liu Yujie Feng Qingliang Zhao 《International Journal of Hydrogen Energy》2009,34(17):7182-7188
Lignocellulosic biomass, if properly hydrolyzed, can be an ideal feedstock for fermentative hydrogen production. This work considered the pretreatment of corn stover (CS) using a dilute acid hydrolysis process and studied its fermentability for hydrogen production by the strain Thermoanaerobacterium thermosaccharolyticum W16. The effects of sulfuric acid concentration and reaction time in the hydrolysis stage of the process were determined based on a 22 central composite experimental design with respect to maximum hydrogen productivity. The optimal hydrolysis conditions to yield the maximum quantity of hydrogen by W16 were 1.69% sulfuric acid and 117 min reaction time. At these conditions, the hydrogen yield was shown to be 3305 ml H2 L−1 medium, which corresponds to 2.24 mol H2 mol−1 sugar. The present results indicate the potential of using T. thermosaccharolyticum W16 for high-yield conversion of CS hemicellulose into bio-H2 integrated with acid hydrolysis. 相似文献
19.
Arunsri FangkumAlissara Reungsang 《International Journal of Hydrogen Energy》2011,36(14):8687-8696
Pre-heated elephant dung was used as inoculum to produce hydrogen from sugarcane bagasse (SCB) hydrolysate. SCB was hydrolyzed by H2SO4 or NaOH at various concentrations (0.25-5% volume) and reaction time of 60 min at 121 °C, 1.5 kg/cm2 in the autoclave. The optimal condition for the pretreatment was obtained when SCB was hydrolyzed by H2SO4 at 1% volume which yielded 11.28 g/L of total sugar (1.46 g glucose/L; 9.10 g xylose/L; 0.72 g arabinose/L). The maximum hydrogen yield of 0.84 mol H2/mol total sugar and the hydrogen production rate of 109.55 mL H2/L day were obtained at the initial pH 6.5 and initial total sugar concentration 10 g/L. Hydrogen-producing bacterium (Clostridium pasteurianum) and non hydrogen-producing bacterium (Flavobacterium sp.) were dominating species in the elephant dung and in hydrogen fermentation broth. Sporolactobacillus sp. was found to be responsible for a low hydrogen yield obtained. 相似文献
20.
《International Journal of Hydrogen Energy》2020,45(8):5202-5215
Biohydrogen is considered as fuel of future owing to its distinctive attribute for clean energy generation, waste management and high energy content. Suitable feedstock play important role for achieving high rate hydrogen production via dark fermentation process. In this regard, different organic wastes such as cane molasses, distillery effluent and starchy wastewater were examined as potential substrates for biohydrogen production by Enterobacter cloacae IIT-BT 08. Groundnut deoiled cake (GDOC) was considered as additional nutritional supplement to enhance biohydrogen yields. The maximum hydrogen yield of 12.2 mol H2 kg−1 CODremoved was obtained using cane molasses and GDOC as co-substrates. To further ensure reliability of the process, bench (50 L) and pilot scale (10000 L) bioreactors were customized and operated. The pilot scale study achieved 76.2 m3 hydrogen with a COD removal and energy conversion efficiency of 18.1 kg m−3 and 37.9%, respectively. This study provides an extensive strategy in moving from lab to pilot scale biohydrogen production thereby, providing further opportunity for commercial exploitation. 相似文献