新型分离技术在中药行业的应用

分析了中药行业的现状和传统中药提取方式的弊端，探讨了新型分离技术--超临界流体萃取技术和膜技术在中草药提取方面的应用前景及存在的问题。     

现代分离技术在挥发油提取中的应用研究

综述了几种现代分离技术在挥发油提取中的应用现状，并和传统提取方法作了简要对比。主要介绍了酶法、破碎法和超临界流体萃取、微胶囊-双水相萃取，并展望了挥发油提取新技术的发展前景。     

现代中药提取分离法

中药制剂现代化很大程度依赖于现代化，其基本原则是继承和发展中药传统配方．以有效的中药复方为对象，运用先进提取分离技术以获取绝大部分有效部位，培养费疗效，减少服用剂量，克服传统中药制剂起效慢、服用量大的弊端。本文对现在用于中药分离提取的几种典型方法进行对比介绍。     

天然香料提取分离技术研究进展

简单介绍了我国天然香料分布情况及其应用领域。综述了高速逆流色谱分离（HSCCC）、超声萃取、超临界二氧化碳、生物催化、分子蒸馏、树脂吸附等技术应用于天然香料提取分离的原理、特点以及在天然香料提取方面的应用。最后对天然香料的提取技术进行展望并提出今后天然香料的发展趋势。     

超临界流体技术在分离工程领域中的应用

文章阐述了超临界流体分离技术的原理、流程与特点.综述了该技术在分离工程领域的应用方向、存在的问题及未来的发展前景.指出了该技术兼具精馏和萃取两种分离作用效果,在天然资源成份的分离提取、脱除有害成份或杂质、清洗、色谱分析等诸多领域部有着其独特的开发应用优势.     

超临界流体萃取技术在精细化工中的应用

本文概述了超临界流体萃取过程及其在精细化工中的若干应用以及分离精制方面新进展。     

绿色分离技术及其在精细化工中的应用

介绍了超临界流体萃取、膜分离、树脂吸附、微波萃取等分离技术的原理、特点以及在精细化工方面的应用.     

天然有机化合物提取与分离技术的研究和应用进展

对天然有机化合物的提取和分离技术进行了综述.对天然有机化合物的提取方法如超临界流体萃取、超声波提取、半仿生提取、酶法提取、微波辅助提取、固相萃取、固相微萃取进行了论述,并对大孔树脂吸附分离、高速逆流色谱分离、膜分离、高速离心分离、分子蒸馏、双水相萃取在天然有机化合物分离上的应用进行了详细论述.     

基于设计思维的研究型教学在化工分离技术课程中的应用

化工分离技术是化学工程与工艺专业的核心课程,多学科交叉、综合性强,理论性与实践性兼重。本文介绍了化工分离技术课程超临界萃取章节教学目标、教学内容与教学方法的改革探索。在绿色化工背景下,基于设计思维的研究型教学理念,利用化工专业软件Aspen,以项目教学的形式完成教学目标并进行教学评价,从而培养学生的文化自信与探索精神,真正发挥信息化教学与研究型教学在新时代化工教育转型中的作用。     

超临界流体及其在萃取中的应用

综述了超临界流体、超临界流体萃取技术及其在工业生产中的应用等,着重介绍了超临界流体萃取的原理和萃取装置,并对超临界流体萃取的发展新趋势作了分析。     

Selection and optimisation of macroporous resin for separation of stilbene glycoside from Polygonum multiflorum Thunb.

BACKGROUND: 2,3,5,4′‐Tetrahydroxystilbene‐2‐O‐β‐glucopyranoside (SG; a stilbene glycoside) is a major bioactive component of Polygonum multiflorum Thunb. (PM), a popular traditional Chinese herb. In this study a method was developed for the separation of SG from PM on macroporous resins. These were selected and optimised using the linear correlation coefficients of the Langmuir and Freundlich isotherms and a pseudo‐first‐order model to explain the kinetic results. Then dynamic adsorption and desorption experiments were carried out on a column packed with the chosen resin to optimise the separation process. RESULTS: HPD100 resin gave the best performance in the separation of SG from PM. The optimal parameters were a sample concentration in solution of 3.5 g mL^?1, a pH of 4.8, a flow rate of 1.5 mL min^?1 and an elution solvent of 40% (v/v) ethanol. Using this scaled‐up column, a product with a final SG content of 819 mg g^?1 and a recovery yield of 74.7% was achieved. In addition, pure acicular crystals of SG could be obtained from this product. CONCLUSION The results showed that HPD100 was the most efficient resin for the separation of SG, which may provide a scientific basis for larger‐scale stilbene glycoside production from PM and other plant extracts. Copyright © 2008 Society of Chemical Industry     

不同采摘期何首乌的红外光谱动态分析及验证

建立不同采摘期何首乌红外光谱动态分析方法。采用红外光谱对不同采摘期何首乌的药材以及醇提物进行测定,并用高效液相色谱法对结果进行验证。随着采摘时间的增加,何首乌的药材及醇提物的红外光谱图相关系数差异逐渐增大,在18个月时达到差异最大,随后差异又减小,液相结果也显示随着时间延长蒽醌类成分的含量也是先逐渐增多,然后再减少,在18个月时达到峰值。所建立的红外光谱方法对何首乌动态分析快速,简便,准确,可用于对不同采摘期何首乌的快速鉴别。     

高速逆流色谱在农药杂质分离中的应用

[方法]利用高速逆流色谱,选用不同的溶剂体系,分别对氟虫腈、氟环唑、咪草烟、氟吗啉原药中的特定杂质进行了分离提纯.[结果]在对应的溶剂体系下,分离得到的杂质纯度均超过90％.达到了杂质定性及定量分析的要求.[结论]HSCCC是一种高效的杂质分离方法.     

Combination of supercritical fluid extraction with high-speed countercurrent chromatography for extraction and isolation of ethyl p-methoxycinnamate and ethyl cinnamate from Kaempferia galanga L.

Supercritical fluid extraction (SFE) with high-speed countercurrent chromatography (HSCCC) was successfully used for the extraction and isolation of ethyl p-methoxycinnamate (EPMC) and ethyl cinnamate (EC) from Kaempferia galanga L. The SFE parameters including extraction temperature, extraction pressure and entrainer volume were optimized by central composite design (CCD). Then the crude extract was separated by HSCCC with a two-phase solvent system composed of n-hexane:ethyl acetate:methanol:water (7:3:8:2, v/v/v/v) in one-step within 60 min. As a result, 13 mg of EPMC and 2 mg of EC were isolated from 100 mg of crude extract with purities of 98.4% and 98.1%, as determined by HPLC. The structural identification was carried out by UV, MS and NMR spectra.     

高速逆流色谱法分离β-谷甾醇和菜油甾醇的研究

利用高速逆流色谱法 ,并选用V(庚烷 )∶V(乙腈 )∶V(乙酸乙酯 ) =5∶5∶1溶剂体系 ,成功地分离了混合植物甾醇标准品中的 β 谷甾醇和菜油甾醇。经过HPLC检测 ,一次性分离可得到质量分数为 97%的 β 谷甾醇和质量分数为 91%的菜油甾醇产品 ,收率分别可达 5 6 %和 5 0 %。该方法简便、重现性好 ,可作为高纯度 β 谷甾醇和菜油甾醇标准品的制备分离方法     

大孔吸附树脂精制鱼腥草黄酮类化合物

Flavonoids are one main kind of effective components in Houttuynia cordata Thunb., which display a wide range of pharmacological activity. In this study supercritical fluid extraction （SFE） with carbon dioxide was first used as preparat ion step to remove the volatile components, which are also active components, from Hout-tuynia cordata Thunb. Then ultrasound-assisted extraction was used to obtain the crude flavonoids and the macro-porous resin adsorption technology was further employed to purify the flavonoids. Nine kinds of macroporous resins with different properties were tested through static adsorption, and one macroporous resin labeled as D101 was selected. The effect of several factors, such as the ratio of column height to diameter, initial concentration and pH, on both flavonoids yield and content were explored by dynamic adsorption to obtain reasonable conditions of adsorption and desorption. The experimental results show that the content of fiavonoids can be above 60% with fia- vonoids recovery of 93.3 % under the optimum conditions of purification. HPLC analysis of the final flavonoids product shows it contains quercitrin, hypefin, rutin and quercetin.     

Separation and Purification of Three High-Purity Isoflavonoids from Belamcanda chinensis (L.) DC. by Supercritical Fluid Extraction and High-Speed Counter-Current Chromatography

Supercritical fluid extraction (SFE) was used to extract three isoflavonoids including irigenin, irisfloretin and dichtomitin from Belamcanda chinensis (L.) DC. The parameters including pressure, temperature, sample particle size, and flow rate of CO₂ were optimized with an orthogonal test. Under the optimized conditions of 15 MPa, 55°C, a sample particle size of 20–40 mesh and CO₂ flow rate of 40 L h^?1. The process was then scaled up by 10 times using a preparative SFE system. The yield of the crude extract from SFE was 4.1%, which contained irigenin, irisfloretin, and dichtomitin 0.71%, 0.49%, and 0.05%, respectively. To compare the extraction methods, Soxhlet Extraction (SE) was performed. The results indicated that SFE was better than SE. Irigenin, irisfloretin, and dichtomitin in the SFE extract were then separated and purified by high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of petroleum ether–ethyl acetate–methanol–water (2:4:3:3, v/v). From 5.0 g of dry crude extract, 27.8 mg irigenin, 16.4 mg irisfloretin, and 2.1 mg dichtomitin were obtained at purities of 97.1%, 96.4%, and 98.0%, respectively, as determined by HPLC-PDA. These results well indicate that SFE and HSCCC are very powerful techniques for the extraction and purification of irigenin, irisfloretin, and dichtomitin from B. chinensis.     

Separation and Purification of Gardecin from Gardenia jasminoides Ellis by High-Speed Countercurrent Chromatography

In the present study, a high-speed counter-current chromatography (HSCCC) was investigated for separation and purification of gardecin on a preparative scale. Hexane–ethyl acetate–methanol–water (0.3:1:0.3:1, v/v) was selected as the optimum solvent system to purify gardecin from a fraction obtained from HPD100 column chromatography fractionation of gardenia. After HSCCC isolation, 20.1 mg of gardecin with purity of 97.4% was obtained from 322 g of dry gardenia fruits. Chemical structure identification of this pigment was carried out by MS, ¹H NMR, and ¹³C NMR. Additionally, antioxidant activity of gardecin in comparison with crocin-1 was investigated. The present results demonstrated that gardecin could be efficiently obtained using HSCCC from this herb and this compound features strong antioxidant activity.     

Supercritical fluid extraction of polymer additives for use in biocompatibility testing

Biocompatibility is the ability of a material to perform with an appropriate host response in a specific application. This response is poorly understood and so there exist inconsistencies in test results both between different laboratories and within the same laboratory. Standard reference materials and test protocols are essential. Analytical-scale supercritical fluid extraction (SFE) has been investigated as a potential standard test protocol for obtaining additives from polymer matrices. These extracts have been analysed for in-vitro cytotoxicity using standard cell culture techniques whose clinical relevance has already been established.