Effect of modified atmospheres on microbiological, color and sensory properties of refrigerated pork

Pork loin samples were stored (4 °C) in nylon polyethylene plastic bags using different modified atmospheres packaging (MAP): vacuum, 100% CO₂ 99% CO₂ + 1% CO, 100% O₂ or 100% CO followed by vacuum. Throughout the storage period Pseudomonas growth was limited in loins packaged in all MAPs evaluated, except for 100% O₂. Psychrotrophs reached 10⁷ CFU g⁻¹ after 20 days of storage except for the loin samples in 100% O₂ MAP that present count above 10⁸ CFU g⁻¹. The 1% CO/99% CO₂ atmosphere was best for preserving the desirable pork loin color and the L* and a* values remained similar to the fresh meat values using this MAP. Pork loins in 99% CO₂/1% CO MAP obtained the highest consumer acceptance scores after 24 h of storage. These samples and those treated with CO and then vacuum packaged received the greatest acceptance scores even after 20 days of storage.     

短波紫外线照射在果蔬和食用菌保鲜中的应用研究

短波紫外线（UV-C）是指波长在200²80nm的紫外线,它能通过杀灭表面微生物、刺激产生非生物胁迫效应等作用改善食品的保鲜品质,而且UV-C处理具有操作简单,投资小,无化学试剂残留等优点,因而成为近年来保鲜领域的研究热点。本文综述了UV-C照射在果蔬和食用菌贮藏保鲜中的研究进展。      

Viscoelasticity, texture and ultrastructure of cut apple as affected by sequential anti-browning and ultraviolet-C light treatments

The aim of this work was to analyze the effect of UV-C radiation (fluence: 11.2 kJ/m²), with or without an anti-browning pretreatment containing 1% (w/v) ascorbic acid plus 0.1% (w/v) calcium chloride, on the linear viscoelastic properties (oscillatory shear and creep/recovery), instrumental texture (TPA), sensory texture and ultrastructure (ESEM, TEM) of cut apple. Changes in structural features and viscoelastic parameters were mainly evidenced after refrigerated storage. All samples showed a viscoelastic solid behavior with the storage modulus (G′) dominating the viscoelastic response. Overall, both dynamic moduli decreased, and instantaneous compliance (J₀), decay compliances (J₁ and J₂) and fluidity significantly increased after treatments and storage at 5 °C, while retardation times were in general constant. Fracture properties proved to be the most highly correlated with sensory texture. The test panel only significantly differentiated stored untreated apple from the other samples regarding fracturability and juiciness. Mechanical spectra and creep parameters showed ability to evidence ultrastructural differences (rupture of membranes, swelling of cells, alteration of cell walls) in the surface of cut apples subjected to the different treatments.     

Effect of storage conditions on nutritional quality and color characteristics of quinoa varieties

To maintain grain quality and prevent loss, effective storage systems are required. The aim of this study was to evaluate the effects of storage duration (0, 60, 120, 180, 240, 300, 360 days) and temperature (4, 10, and 25 °C) on proximate and nutritional components, and color properties of Mint Vanilla and Titicaca quinoa varieties. The results showed that the increase in storage duration and temperature leads to changes in the grain moisture, protein and ash contents, nutritional component and color properties. There was an increase in ash content at 25 °C, moisture content at 10 and 25 °C, and protein content at 4 °C in the stored grains compared with the grains on the initial of storage for Mint vailla variety. Whereas, there was an decrease in ash and protein content for all the temperature at the end of 360 days storage for Titicaca variety. Overall, there was a decrease (except Fe, Zn, Co, Ni, Cd and Pb) in the nutritional component of both quinoa varieties. L1, H° and WI values decreased, and a1, b1 and C1 values increased as a function of storage duration and temperature and showed some temperature-dependent degradation for both quinoa varieties.     

The use of biodosimetry to measure the UV-C dose delivered to a sphere, and implications for the commercial treatment of fruit

Commercialization of UV-C treatment of horticultural produce in order to induce beneficial responses in the produce following treatment requires both accurate dose delivery and a method of treating large quantities of produce efficiently. Furthermore, it has long been assumed that such effects require the entire surface of the horticultural commodities - typically fruit - to be exposed to UV-C. This has invariably been achieved by manually rotating the fruit in a UV-C field whilst reducing the dose delivered at each rotation in direct proportion to the number of rotations. However, the resulting UV-C dose distributions achieved under these circumstances are generally not reported in the literature. In the work described here a polystyrene sphere (dia. 70 mm) was used to simulate fruits such as tomatoes, apples, peaches, etc., that have an approximately spherical form in order to provide a means of measuring the total doses of UV-C accumulated during treatment and comparing such estimates to theoretically-derived ones. This was achieved using dosimetry based on spores of Bacillus subtilis in which spore-impregnated membranes were attached to the surface of the sphere. The fraction of spores surviving exposure was used to estimate dose from a dose-response curve for the spores. Under irradiation conditions leading to a theoretically calculated dose of 10.6 J, spore dosimetry yielded estimates of 9.1, 10.7 and 6.1 J for UV-C delivered in, respectively, one, two or four exposures. In the case of exposure of the sphere during continuous mechanical rotation for the same length of time (80 s) a value of only 3.5 J was obtained. Irradiation conditions resulting in the spores being subject to intermittent exposure to UV-C led to dose estimates below the theoretically derived ones. The circumstances under which spore dosimetry can be used to obtain surface dose distributions are discussed.     

短波紫外线法处理对鲜切果蔬抗氧化系统影响的研究进展

短波紫外线（ultraviolet-C,UV-C）作为一种绿色无污染的非热处理技术,近年来在鲜切果蔬保鲜中的研究越来越多,主要研究热点在UV-C对鲜切果蔬抗氧化系统的影响。鲜切加工、贮藏、运输、销售等过程会引发一系列的不利于果蔬贮藏的生理生化和品质特性改变,导致其食用品质和商用价值下降,带来重大的经济损失。鲜切果蔬抗氧化系统主要由酚类物质、黄酮类物质、维生素类等物质组成非酶抗氧化系统,超氧化物歧化酶、过氧化氢酶、过氧化物酶、抗坏血酸氧化酶等组成酶促抗氧化系统,共同发挥对鲜切果蔬贮藏品质的调节作用。本文介绍了UV-C和鲜切果蔬的特点,综述了UV-C对不同鲜切果蔬中非酶抗氧化系统和酶促抗氧化系统的影响,并对未来研究前景进行了展望,旨在为探究果蔬鲜切伤害条件下,UV-C处理对果蔬抗氧化系统响应机制的影响和鲜切果蔬贮藏品质特性影响的机理,并为UV-C在鲜切果蔬上的进一步推广、应用提供参考。     

Change of polyphenol oxidase activity, color, and browning degree during storage of cloudy apple juice treated by supercritical carbon dioxide

The effects of supercritical carbon dioxide (SCCO₂) treatment of 8, 15, 22, and 30 MPa for 60 min at 55 °C on polyphenol oxidase (PPO) activity, color, and browning degree in cloudy apple juice during storage at 4 °C for 4-weeks were investigated. The SCCO₂ treatment had significant effects on inactivation of PPO and the least residual activity of PPO was 38.50% at 30 MPa. The restoration of PPO residual activity after SCCO₂ treatment was also observed, which was dependent on the pressure level. A greater reduction of lightness L and a minor increase of redness a of cloudy apple juices after SCCO₂ treatment occurred. Moreover, the total color difference (ΔE), which was significantly less than that of untreated sample, was decreased by enhancing the pressure level. The changes of lightness L and browning degree A during storage were well fitted to a first-order kinetic model. The rate constants of k _L and k _A of samples subjected to SCCO₂ treatment reduced from 4.75×10⁻² to 0.42×10⁻² and 37.19×10⁻² to 8.02×10⁻², respectively, when pressure increased from 0 MPa (untreated sample) to 30 MPa.     

Control of microbial growth, browning and lipid oxidation by the spice Aframomum danielli

Aframomum danielli   (family Zingiberaceae) for Listeria monocytogenes LCDC 81861 was 390 ppm. No viable cells were found after a 4 h exposure of Bacillus cereus (initial concentration 10³ cfu/ml) to 500 ppm of A. danielli (aqueous solution) when the concentration of untreated control cells was 3.9×10⁶ cfu/ml. The MICs of A. danielli for some food spoilage yeasts (Candida tropicalis, Hansenula anomala, Torulopsis candida and Kluveromyces thermotolerans) ranged from 100 to 200 ppm. Exposure of McIntosh apple slices at 25°C for 4 h to a crude powder of A. danielli (17000 ppm) and ascorbic acid (5000 ppm) produced comparative increased L* values and decreased a* values (antibrowning effect) as measured by reflectance spectroscopy. When crude antioxidant extract from A. danielli was added to refined soybean oil at 200 ppm, the antioxidant effectiveness (AE) was 70.7%, calculated from:

Effects of salt and ammonium hydroxide on the quality of ground buffalo meat

The objective of this study was to evaluate the effects of ammonium hydroxide (AH) and sodium chloride on the quality of ground buffalo meat patties. Ground buffalo meat was treated with distilled water (control), 0.5% v/w AH, 1.0% v/w AH, 2.0% v/w AH and 1.0% w/w sodium chloride was added for all the samples. Treatment with AH increased (P<0.05) the pH and water holding capacity (WHC) of ground buffalo meat patties during storage relative to their controls. Hunterlab a* (redness) and chroma values increased (P<0.05) and hue decreased (P<0.05) in all AH treated samples in comparison to controls during storage. Ammonium hydroxide significantly (P<0.05) inhibited metmyoglobin formation compared to control after 3rd day of storage. There was a significant (P<0.05) reduction in thiobarbituric acid reactive substances (TBARS) values in all AH treated samples compared to control throughout storage. These results indicate the potential antioxidant and myoglobin redox stabilizing effect of AH in ground buffalo meat patties.     

Effect of substitution of stevioside and sucralose on rheological,spectral, color and microstructural characteristics of mango jam

An attempt was made for manufacturing low calorie mango jam by replacement of sucrose with alternative sweeteners (stevioside and sucralose). Manufacture of mango jam with desired jam-like soft solid characteristics was feasible only with 25% stevioside or sucralose substitution. Herschel Bulkley and Hahn model adequately described the steady state and time dependent rheological behavior of stevioside/sucralose jam, respectively. Consistency index and yield stress values of jam samples decreased with increasing substitution of stevioside or sucralose due to reduction of total soluble solids (TSS). Flow behavior index increased with decreasing TSS values, signifying liquid like behavior of jam. Dynamic rheological tests characterized mango jam as a weak gel. L^∗ (lightness), b^∗ (yellowness) and C^∗ (chroma) values increased with stevioside/sucralose substitution in jam. The spectral features for C–C and C–O stretching indicated its importance in ‘gel’ like structure formation due to hydrophobic interaction in mango jam samples.     

柠檬酸缓冲液对蒸谷米色泽及品质特性的影响

采用不同pH的柠檬酸缓冲液浸泡稻谷,以纯水浸泡的蒸谷米为对照组,考察了不同处理组蒸谷米的色差、加工品质、感官评价、体外消化、热力学特性和质构特性的变化,并进行了相关性分析。结果表明：低pH浸泡液预处理后,蒸谷米色差有显著变化,其中pH 4浸泡液颜色最浅,相对纯水浸泡的蒸谷米,其L^*值增加了5.27;消化结果显示,样品淀粉消化率随pH降低而降低,其中pH 3组淀粉消化率最低,其eGI值降低了11.83%,抗性淀粉增加了29.06%;加工品质结果显示,pH对出米率无显著影响,但随着浸泡液pH降低整精米率有所降低、爆腰率有所上升;感官评价和质构特性结果表明,蒸谷米的食用品质随着pH的降低先上升后下降。综合结果表明,适当低pH浸泡液预处理对蒸谷米的色泽、体外消化特性、感官品质具有一定的改善作用。     

Impact of muscle type and sodium chloride concentration on the quality, sensory, and instrumental color characteristics of solution enhanced whole-muscle beef

Beef biceps femoris (n = 10; muscle sections, n = 20; BF), infraspinatus (n = 10; muscle sections, n = 20; IS), and longissimus (n = 10; muscle sections, n = 20; LM) muscles were utilized to evaluate the effect of enhancement with phosphate and varying levels of sodium chloride in beef muscles differing in composition and palatability. Muscles were untreated or solution enhanced to 112% of raw product weight with sodium tripolyphosphate at 0.4% of product weight (STPP), or STPP and sodium chloride (NaCl) at 0.5%, 1.0% or 1.5% of product weight. There was a quadratic relationship (P = 0.04) for percent free water to decrease and a linear relationship (P < 0.01) for cook yield to increase as the level of NaCl increased. The IS steaks required less (P < 0.05) shear force than either the BF or LM, with the BF and LM having similar (P > 0.05) shear force values. There was a linear relationship (P < 0.01) for shear force values to decrease with increasing salt concentration. Steaks from all four enhancement treatments had lower (P < 0.05) shear force values than untreated steaks. Sensory overall tenderness ratings revealed that the IS and LM were similar (P > 0.05) and superior (P < 0.05) to the BF in tenderness. Steaks enhanced with STPP and 0.5%, 1.0%, or 1.5% NaCl all were rated more tender (P < 0.05) than untreated or STPP-only treated steaks. The BF exhibited the lowest (P < 0.05) L* (lightness) and a* (redness) values, as well as decreased (P < 0.05) vividness. The LM generally exhibited superior color to the other two muscle types. There was a linear relationship (P < 0.01) for L*, a*, b* (yellowness) values, and vividness to decline with increasing salt concentration, but steaks enhanced with STPP and 0.5% NaCl were similar (P > 0.05) in a* values and vividness to untreated steaks. These results suggest that across three different muscles varying in composition and palatability, enhancement with 0.4% STPP and 0.5% NaCl allowed for improvements in palatability while minimizing the color deterioration associated with phosphate/salt enhancement.     

Effect of dielectric microwave heating on the color and antiradical capacity of betanin

The decomposition of betanin under dielectric heating (microwave irradiation, power: 25–200 W (3–24 kJ g⁻¹)) follows first-order kinetics with a rate constant similar to that obtained during conventional conduction heating (half-life < 2 min at 100 °C). Color coordinate analysis indicates that betanin is bleached upon thermal treatment, whereas beetroot juice and spray-dried beetroot powder tend to form colored decomposition products. The antiradical capacity of betanin decreases upon heating, but is still much higher than that of standard antioxidants such as ascorbic acid and trolox. Betalamic acid, a high capacity antiradical, was detected by mass spectrometry and second-derivative absorption spectroscopy in betanin samples submitted to thermal treatment.     

Effect of quality, porosity and density on the compression properties of cork

The compression properties of cork were studied on samples obtained from cork planks of two commercial quality classes (good and poor quality), with densities ranging from 0.12–0.20g?cm^-3 and porosities from 0.5 to 22.0%. The stress-strain curves were characterized by an elastic region up to approximately 5% strain, followed by a large plateau up to 60% strain caused by the progressive buckling of cell walls, and a steep stress increase for higher strains corresponding to cell collapse. The direction of compression was a highly significant factor of variation, with cork showing higher strength for the radial compression. Density influenced compression and cork samples with higher density showed overall larger resistance to compression in the three directions. In the elastic region, an exponential model of Young’s modulus in function of cork density could be adjusted. The effect of porosity on compression was small and the stress-strain curves were similar regardless of the porosity of the samples, although there was a trend toward an overall increase of stress with porosity for higher strains. Porosity was characterised by a high variability in the anatomical features of the lenticular filling material and the presence of collapsed and thick walled lignified cells. The inclusion of a porosity parameter for the modelling of the elastic modulus did not improve the prediction obtained with density-based models. There was no significant difference in the compression properties of cork samples obtained from cork planks of good and poor quality classes.     

Effect of light and sweeteners on color in an amaretto-type liqueur

Studies on the color loss in an amaretto-type liqueur under controlled light conditions showed a clear dependence of the decoloration rate on the light intensity, and complete color stability in the absence of light. The principal sweetener used in the preparation of the liqueur strongly affected the rate of color loss under irradiation, color stability being much greater for the formulations containing sucrose or no added sweetener instead of fructose 42. These differences were more pronounced in experiments conducted with chemically well-defined mixtures that contained either of the 2 azo dyes used in the coloration of the amaretto, tartrazine, and Allura Red, and various alternative sweeteners, in 28% (v/v) ethanol solution: D-fructose and, to a lesser extent, D-glucose, at concentrations of 14% (w/v), were effective in bringing about photodecoloration, while no color loss was detected in the presence of sucrose, or in the absence of any added sugar. The results are interpreted in terms of a redox reaction of reducing sugars with the diarylazo compounds, the function of the light being the conversion of the azo compound from the predominant trans configuration to the cis configuration, which on geometric grounds lends itself better to a concerted, cyclical redox reaction with the reducing sugar.     

Effect of carbon monoxide in modified atmosphere packaging, storage time and endpoint cooking temperature on the internal color of enhanced pork

The objective of this research was to evaluate the effects of gas atmosphere, refrigerated storage time, and endpoint temperature on internal cooked color of injection-enhanced pork chops. Enhanced chops were packaged in 0.36% CO/20.34% CO₂ (CO-MAP), 80% O₂/20% CO₂ (HO-MAP), or PVC-overwrapped (PVC-OW; controls), stored at 4 °C for 0, 12, 19 or 26 days, displayed for 2 days then cooked to six endpoint temperatures (54, 60, 63, 71, 77, and 82 °C). L^*, a^*, and b^* values, hue angle and chroma were determined on the internal cut surface of cooked chops. Chops packaged in CO-MAP had the highest a^* values; a^* value began increasing on day 14. The lowest hue angles occurred in chops cooked to lower endpoint temperatures. Chops in CO-MAP had lower hue angles and higher chroma than those in HO-MAP and PVC-OW. Above 71 °C, hue angle and chroma increased. Overall, CO-MAP packaged chops stored for longer time periods then cooked to lower endpoint temperatures appeared reddest. HO-MAP packaged chops were less red, did not change over time, and appeared more well done at lower endpoint temperatures than those in other gas atmospheres. CO-MAP packaged chops retained redness even after cooking at 82 °C.     

Effects of pretreatments on quality attributes of long-term deep frozen storage of vegetables: a review

As one of the essential parts in human diet, vegetables are important in health keeping and their consumption increases continuously. Due to their highly perishable nature, the shelf life of fresh vegetables is considerably short, due to cellular respiration, microorganism, enzyme reaction, oxidation and so on. Therefore, short- and long-term storages of vegetables are required and various methods and technologies are applied for different commercial goals. For long-term storage, deep frozen storage is one of the most widespread used preservation methods for vegetables, as under temperatures low enough, the rate of most deteriorative reactions and microbial activities are significantly reduced. This review provides a critical comprehensive summary of long-term storage (≥6 months) vegetables under low temperatures (≤??18?°C), and effects of the storage methods on various quality attributes of vegetables, such as texture, colour, contents of ascorbic acid, chlorophyll and carotenoids. Besides, the impacts of common pretreatments prior to freezing on the subsequent frozen storage are also briefly discussed. The current review shows that although some important biochemical attributes are more or less deteriorated and the quality loss of them is inevitable, a substantial portion of quality attributes appear to be stable during long-term deep frozen storage especially the physical parameters. Meanwhile, pretreatments prior to freezing, such as blanching process, also show significant influence on quality preservation in subsequent storage. Therefore long-term deep frozen storage can be applied as an effective storage method under proper conditions of pretreatments and storage.