采用微囊化技术研制新型高效双歧杆菌活菌制剂

采用流化床技术与喷咀结合的微型包囊法,将三种双歧杆菌、高效活菌保护剂和双歧促生因子一起作为核心物质,包封于无毒囊材中制成耐酸肠溶微囊（或微球）。微囊在人工模拟胃液（ｐＨ１．５～２．０）中处理２ｈ,其中的双歧杆菌活菌数仍保持在９０％ 以上;在人工肠液中处理１５ｍ ｉｎ,微囊全部崩解释放出活性菌,在３７℃下保存３ 个月（相当于常温下一年）,其中的活菌数仍大于１０９ 个／ｇ。用上述耐酸肠溶微胶囊制成的新型高效微生态活菌制剂和功能食品在经口服时,其中的活性双歧杆菌能顺利通过人体胃和小肠,到达大肠释放,从而有效地解决了胃酸、消化酶和抗生素等对活性双歧杆菌引起的灭活问题和产品常温保存期太短的问题。     

采用微囊化技术研制新型高效双歧杆菌活菌制剂

采用流化床技术与喷咀结合的微型包囊法,将三种双歧杆菌、高效活菌保护剂和双歧促生因子一起作为核心物质,包封于无毒囊材中制成耐酸肠溶微囊（或微球）。微囊在人工模拟胃液（ｐＨ１．５～２．０）中处理２ｈ,其中的双歧杆菌活菌数仍保持在９０％ 以上;在人工肠液中处理１５ｍ ｉｎ,微囊全部崩解释放出活性菌,在３７℃下保存３ 个月（相当于常温下一年）,其中的活菌数仍大于１０９ 个／ｇ。用上述耐酸肠溶微胶囊制成的新型高效微生态活菌制剂和功能食品在经口服时,其中的活性双歧杆菌能顺利通过人体胃和小肠,到达大肠释放,从而有效地解决了胃酸、消化酶和抗生素等对活性双歧杆菌引起的灭活问题和产品常温保存期太短的问题。      

两歧双歧杆菌微胶囊化及其性质研究

以明胶、果胶、海藻酸钠、氯化钙和壳聚糖为壁材,采用乳化法双层包埋制备双歧杆菌微胶囊。制备的微胶囊粒径在10～30μm。检测结果表明,微胶囊内活菌数可达到109 CFU/g以上,菌体包埋率可达到82.24%。经模拟胃酸、胆汁酸处理后活菌数仍在108 CFU/g以上,对酸有很高的耐受力;经人工肠液处理15min,微胶囊几乎全部崩解,肠溶释放率可达到95.81%。通过经典的加速实验证明微胶囊的活菌贮藏稳定性较好,室温下贮藏1年其活菌数仍可以保持在108CFU/g以上。     

免疫初乳与双歧杆菌复合微胶囊的加工特性

采用空气悬浮微胶囊化方法制成免疫与微生态双活性的免疫初乳与双歧杆菌复合微胶囊,对其加工特性进行研究,包括复合微胶囊的溶解性、耐酸性、耐胆汁盐性及稳定性。结果表明:研制成的微胶囊在人工胃液中2h不崩解,而在人工肠液中40min时崩解完全;人工胃液处理2h后,活菌存活率达87%,IgG的活性仍可达到28,表观回收率达90.5%;在高胆汁盐溶液中处理3h后,活菌数仍可以达到67.7%,IgG活性为27,仅下降了1个滴度;在室温下贮存10个月,双歧杆菌的存活率仍可达31.7%,活菌数仍在108CFU/mL以上;IgG活性没有发生变化,仍为28。     

采用微型包囊技术研制活性双歧联菌微囊微生态制剂

针对双歧杆菌活菌制剂在口服时,不耐受强胃酸,活性菌在有氧条件下容易失活,常温下保存期短等存在问题,将微型包囊技术应用于微生态制剂中,并已研制成活性双歧联菌株耐酸肠溶微囊微生态制剂, 获得较理想的效果.本文介绍了双歧联菌株混合培养工艺条件的优化研究, 活性双歧杆菌冻干保护剂和冻干条件的确定, 微型包囊复合囊材配方的优化研究, 最佳微型包囊方法的确定及微囊化条件的研究, 含活性菌微囊的耐酸肠溶试验, 含活性菌微囊的保存稳定性试验, 50升发酵罐中试结果,以及活性双歧联菌株微囊微生态制剂的研制等.     

采用微型包囊技术研制活性双歧联菌微囊微生态制剂

针对双歧杆菌活菌制剂在口服时，不耐受强胃酸，活性菌在有氧条件下容易失活，常温下保存期短等存在问题，将微型包囊技术应用于微生态制剂中，并已研制成活性双歧联菌株耐酸肠溶微囊微生态制剂，获得较理想的效果。本文介绍了双歧联菌株混合培养工艺条件的优化研究，活性双歧杆菌冻干保护剂和冻干条件的确定，微型包囊复合囊材配方的优化研究，最佳微型包囊方法的确定及微囊化条件的研究，含活性菌微囊的耐酸肠溶试验，含活性菌微囊的保存稳定性试验，50升发酵罐中试结果，以及活性双歧联菌株微囊微生态制剂的研制等。     

冻干乳酸双歧杆菌微胶囊的制备及存活性能研究

为了提高双歧杆菌在贮存和消费过程中的菌体存活率,利用冷冻干燥与微囊化结合的方法,将双歧杆菌和高效活菌保护剂一起作为核心物质,采用乳化法制备双歧杆菌微胶囊。通过正交试验,对双歧杆菌冻干过程中加入的保护剂种类及其最佳配比进行了研究。并探讨了双歧杆菌在模拟胃肠道环境以及高渗透压等极端环境条件下的存活能力。结果表明：加入10%菊糖、6%大豆蛋白、12%海藻糖、10%甘露醇可显著提高微囊化双歧杆菌冻干活菌数,使其存活率达到69.2%。较之冻干菌粉,双歧杆菌微囊在模拟胃液、肠液、高胆汁盐以及高渗透压溶液中均具有较好的耐受性。     

微囊化双歧杆菌贮藏过程活菌数的变化

微囊化双歧杆菌是一种新型高效微生态制剂。本研究了在微囊化双歧杆菌制品保藏过程中水分、原始菌数、保护剂、温度等因素对双歧杆菌活菌总数的影响。     

双歧杆菌混凝胶微囊制备及其胃肠释放特性研究

为克服双歧杆菌不耐酸、不耐氧的缺点,本试验建立制备双歧杆菌微胶囊的方法。以海藻酸钠和氯化钙形成海藻酸钙水凝胶,再与壳聚糖复合形成聚电解质膜,通过单因素及Box-Behnken响应面试验优化制备双歧杆菌混凝胶微囊工艺,使用扫描电镜观察胶囊结构,并采用模拟胃液及肠液的独立影响实验和模拟连续消化道实验考察胶囊中双歧杆菌的释放特性及存活率,探究其稳定性。结果表明:离心菌体与1.5%海藻酸钠均匀混合,注射到1.2%氯化钙溶液中,再与0.5%壳聚糖固定化35 min最佳。此条件下胶囊形状完整,扫描电镜图显示微胶囊结构致密、具有较好的包埋性,胃肠释放实验证明胶囊具有肠溶胃不溶和缓释的载体特性,胶囊中的双歧杆菌在肠液中的存活率平均值为72.3%,经模拟消化道处理后的存活率为49.8%。此结果为双歧杆菌微胶囊化提供参考。     

长双歧杆菌BBMN68微胶囊的制备及其应用性评价

长双歧杆菌BBMN68分离自广西巴马长寿老人粪便,已证明其具有双歧杆菌普遍的生理功能。为解决其不耐酸、不耐氧的缺点,本实验采用微胶囊包埋技术,以海藻酸钠、乳清蛋白以及VE为壁材,在氮气体系中采用挤压法包埋该双歧杆菌活菌体制备湿微胶囊和冻干微胶囊。结果表明：海藻酸钠、乳清蛋白和VE质量分数分别为2%、3%和10%条件下制备的湿微胶囊在酸奶中保存21 d仍能保持106 CFU/g以上的活菌数,耐胃酸实验2.0 h时活菌数仍高达3.16×107 CFU/g,肠溶实验中微胶囊在2.0 h实现完全崩解;海藻酸钠和乳清蛋白质量分数为2%和3%,氮气环境下制备的冻干微胶囊通过恒温加速实验测定可在室温条件下贮藏长达75 d。通过微胶囊包埋技术较好地解决了长双歧杆菌BBMN68不耐酸、不耐氧的缺点,同时也提供一种新型有效的补充肠道益生菌的形式。     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the     

Chinese CTP Market

According to Printing and Printing Equipment Industries Association of China（PEIAC）＇s statistics to the plate manufucturer in China, in 2013, the actual offset plate production has reached 346 million square meters in China. Among them, the CTP production volume was 245 million square meters, up by 11% than that of last year; the total sales of the CTP plate was 239 million square meters, up by 13%.     

Preparatory Work of Print China 2015 is Going Smoothly

正Held at Guangdong Modern International Exhibition Center,Print China 2015 will cover 7exhibition halls,besides the original Hall No.3,4,5,6,7,the newly built F zone of Hall 3 will be used too.The total area will be140,000 square meters.Hall 3:Offset and large printing equipment,package printing equipment,post press