低双乙酰啤酒酵母的选育

将APV啤酒酵母菌株在不同的双乙酰含量梯度培养基内培养，挑出抗双乙酰的变异菌株，经菌种分离、筛选、发酵及双乙酰驯养等步骤选育出一株7^#菌，11℃低温发酵，双乙酰峰值0．36mg／L，成品啤酒双乙酰含量0．06mg／L，真正发酵度66．1％。     

啤酒酵母融合株QSB—Ⅺ6的生产性试验

ａｃｃｈａｒｏｍｙｃｅｓＣｅｒｅｖｉｓｉａｅＱＳＢ－Ⅺ６菌株系酵母菌种ＬＱ１６与ＱＳＢ７的灭活原生质体融合株。ＱＳＢ－Ⅺ６菌株应用于车间生产中，成品酒的各项理化指标均已达到国家优质酒标准，其中双乙酰值平均在０．０６ｍｇ／ｌ以下，真正发酵度平均为７１．８５８％。该融合株凝聚性强，酒液口味独特，清淡爽口。经多次重复试验证明，该菌株各项优良性状稳定，非常适合于规模化生产。     

黑曲霉(Aspergilus niger)F_(27)固体曲提高啤酒糖化利用率及对啤酒质量影响的研究

黑曲霉Ｆ２７是华中农业大学生科院发酵工程室选育的一株能产较高的β—葡聚糖酶及酸性蛋白酶的菌株。本研究利用黑曲霉Ｆ２７固体曲作为复合酶制剂用于啤酒糖化，从而提高原料糖化利用率，改善麦汁组成，提高啤酒质量。结果表明，以二级麦芽为原料，利用该固体曲，原料利用率提高５．１４％。１０°Ｂｘ麦汁的α－氨基氮从１６３ｍｇ／Ｌ提高到２０ｍｇ／Ｌ，还原糖从７．６ｇ／１００ｍｌ提高到８．１ｇ／１００ｍｌ，麦汁发酵情况正常，成品酒经农业部食品检验测试中心分析及国家和湖北省评酒委员品评，其理化指标全部符合有关国家标准，风味上与正常工艺生产的啤酒无差异     

高发酵度啤酒酵母生产试验研究

高发酵度啤酒酵母生产试验研究王佐民，索晓光，胡晓东酿酒，１９９６（２）：２２～２４采用生物技术，选育分离出１株高发酵度啤酒酵母菌株ＳＬ－８。该菌株具有双乙酰还原能力强、发酵度高等特点。将该酵母菌株用于生产试验，其成品酒实际发酵度在７５％以上，适合较高...     

黑曲霉（Aspergillus niger）F27固体曲提高啤酒糖化利用率及对啤酒质量影响

黑曲霉Ｆ２７是华中农业大学生科院发酵工程室选育一株有产较高的β－葡聚糖酶及酸性蛋白酶的菌株，本研究利用黑曲霉Ｆ２７固体曲作为复合酶制剂用于啤酒糖化，从而提高原料糖化利用率，改善麦汁组成，提高啤酒质量。结果表明，以二级麦芽为原料，利用该固体曲，原料利用率提高５．１４％，１０＾Ｂｘ麦汁的α－氨基氮从１６３ｍｇ／Ｌ提高到２０ｍｇ／Ｌ，还原糖从７．６ｇ／１００ｍｌ提高到８．１ｇ／１００ｍｌ，麦汁发酵情况正     

紫外诱变及苯黄隆抗性处理选育低双乙酰啤酒酵母

将APV菌株经紫外线诱变后筛出苯黄隆抗性菌株,经实验室菌种分离、筛选、发酵及双乙酰驯养等步骤,筛选育出一株2#菌株,发酵双乙酰峰值为0.36mg/L,成品啤酒双乙酰含量为0.07 mg/L,真正发酵度为65.8%.     

国内期刊文摘

发泡酒酿造用贫氮酵母性质的研究陈阿扣，顾国贤，陆健陈阿扣，顾国贤，陆健酿酒，２００２，（２）：５１－５４．以α－氨基氮利用率高、双乙酰峰值低为筛子，筛选到贫氮酵母菌株。贫氮酵母在贫氮麦汁中能利用少部分的脯氨酸和相当部分的麦芽三糖，发酵后的啤酒中总氮量符合淡爽啤酒的要求，是一株中等凝聚性的酵母。１１P发泡酒贫氮麦汁发酵结果为：发酵度６４．０％，双乙酰０．０８４mg／L，发泡酒中含氮量２９７mg／L。（津京）葡萄籽中的天然抗氧化剂及其保健功能林亲录，施兆鹏，秦丹食品与发酵工业，２００２（４）：７５－７８…     

啤酒酵母融合株QSB—Ⅺ6中试研究

将新选育的酵母融合株ＳａｃｈａｒｏｍｙｃｅｓｃｅｒｅｖｉｓｉａｅＱＳＢ－Ⅺ６,同本厂生产中使用的酵母菌种ＳａｃｈａｒｏｍｙｃｅｓｃｅｒｅｖｉｓｉａｅＬＱ１６在２３０Ｌ发酵罐中做对比性发酵试验。试验酒的各项理化指标均已达到国家优质酒标准,其中双乙酰值在００８ｍｇ／ｌ以下,真正发酵度平均为７０２０％,酒体泡沫丰富,清亮透明。口味纯正,风味独特,为使该菌种能更好地应用于生产,奠定了基础。     

黑曲霉液体发酵纤维素酶的研究

在黑曲霉ＤＭ—１液体深层发酵所产纤维素酶系中，β－葡萄糖苷酶活性特别高。系统研究了ＤＭ—１菌株的摇瓶产酶条件及２５Ｌ发酵罐发酵工艺。２５Ｌ发酵罐试验结果表明，在通风量０．４～１．０ｖｖｍ、搅拌转速２５０～５００ｒ／ｍ、发酵温度３１℃及控制发酵液ｐＨ在４．０左右的条件下，发酵１０４小时，其β－葡萄糖苷酶活和ＣＭＣ分别为３３０和２４１ｍｇ葡萄糖／ｍｌ。发酵滤液经硫铵盐析沉淀、过滤或离心及干燥等过程得固体纤维素干酶粉。其中β－葡萄糖苷酶活为１３５００ｍｇ葡萄糖／ｇ，平均收率８０．２％。     

二种重组α-乙酰乳酸脱羧酶在啤酒生产中降低双乙酰的作用

利用已构建的含有短芽胞杆菌（Ｂａｃｉｌｌｕｓｂｒｅｖｉｓ）和产气肠杆菌（Ｅｎｔｅｒｏｂａｃｔｅｒａｅｒｏｇｅｎｅｓ）、α -乙酰乳酸脱羧酶（α -ａｃｅｔｏｌａｃｔａｔｅｄｅｃａｒｂｏｘｙｌａｓｅ,α -ＡＬＤＣ）基因的工程菌株 ,并使它们分别在大肠杆菌中高效表达 ,获得重组α -ＡＬＤＣ。在实验室 ,用２Ｌ体积的麦芽汁进行啤酒生产试验 ,添加２种重组α -ＡＬＤＣ后 ,使啤酒中的双乙酰含量快速下降到或始终保持在０．１ｍｇ／Ｌ以下。证明得到的重组α -ＡＬＤＣ能有效地降低啤酒中双乙酰含量。这２种重组α -ＡＬＤＣ可进一…     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the     

Chinese CTP Market

According to Printing and Printing Equipment Industries Association of China（PEIAC）＇s statistics to the plate manufucturer in China, in 2013, the actual offset plate production has reached 346 million square meters in China. Among them, the CTP production volume was 245 million square meters, up by 11% than that of last year; the total sales of the CTP plate was 239 million square meters, up by 13%.     

Preparatory Work of Print China 2015 is Going Smoothly

正Held at Guangdong Modern International Exhibition Center,Print China 2015 will cover 7exhibition halls,besides the original Hall No.3,4,5,6,7,the newly built F zone of Hall 3 will be used too.The total area will be140,000 square meters.Hall 3:Offset and large printing equipment,package printing equipment,post press