Performance Analysis of a Continuous Rotating Annular Electrophoresis Column

Abstract

A new continuous electrophoresis column is proposed which will allow for the continuous separation of industrial scale multicomponent mixtures. Computer simulated calculations predict steady-state bed temperature, separation distance, and power requirements. The proposed design compares favorably to previous designs in terms of minimizing peak bed temperature and power consumed.     

Fractional Cleanout in a Continuous-Flow Centrifuge

Abstract

A theoretical equation is derived for the fraction of particles removed from the feed stream of a continuous-flow centrifuge for the case of an arbitrary velocity profile in the centrifuge. A definition of the fractional cleanout is adopted which is somewhat different from that used in earlier work, and it leads to a simpler final equation.     

高效毛细管电泳的进展

自1988年第一台商品化的毛细管电泳仪（CE）问世,距今已有二十多年的时光。在这期间,CE技术无论在理论、仪器还是应用等方面都得到飞速发展,新技术不断涌现,应用领域不断扩大。本文在SCI检索的基础上,重点对1995年以来CE技术在上述领域的最新研究进展进行综述。     

Fluid Flow Characteristics of Forced Flow Electrophoresis

Abstract

Fluid flow in forced flow electrophoresis has not been previously analyzed, even though it presents some interesting aspects. The effectiveness of this method for biological separations is due to a superimposition of an electric field on filtration. A mathematical model is presented, describing fluid flow and mass transfer for dilute solutions at electrical potentials less than the critical one. The calculated solute trajectories in a channel are determined by the ratio of the electrophoretic velocity to the withdrawal velocity through the permeable wall. The stationary layer and the layer in which all the solutes arrive at the permeable membrane at the end of the channel are also calculated. The concentration of the filtrate through the permeable membrane is obtained from the material balance of the solute entering the channel. Increased performance is obtained by means of a double-stage forced flow electrophoresis, where the ratio of final filtered solute concentration to inlet concentration is shown to be the square of the same ratio at the first stage.     

高效毛细管电泳法测定乳氟禾草灵的含量

建立了高效毛细管电泳法测定乳氟禾草灵的含量.使用有效长度为42 cm、内径为50 μm的毛细管,在背景电解质为50 mmol/L硼砂-150 mmol/L磷酸二氢钠(pH值9.5)、检测波长为281 nm、分离电压为15 kV的优化条件下,分析时间小于6 min.乳氟禾草灵质量浓度在10～240 mg/L内线性关系良好,相关系数为0.9997;方法的检测限(S/N为3)和定量限(S/N为10)分别为0.8、2.0 mg/L;加标回收率为98.2%～99.0%,相对标准偏差为0.59%～2.6%.     

毛细管电泳法测定水飞蓟宾的含量

采用高效毛细管电泳法(HPCE)测定水飞蓟宾的含量.以未涂敷熔融石英毛细管柱(50 cm×75μm,有效长度45 cm)作为分离通道,缓冲溶液为10 mmol·L-1硼砂(pH为6.0),分离电压为15 kV,检测波长288 nm,虹吸进样,高度差10 cm,进样10 s,柱温25℃的条件下,水飞蓟宾分析时间在4 mi...     

Facile and Rapid Synthesis of Neopentyl Glycol in a Continuous-Flow Microreactor

Neopentyl glycol (NPG) synthesis by condensation disproportionation of formaldehyde and isobutyraldehyde (IBD) with sodium hydroxide as catalyst was studied in a continuous-flow microreactor under various experimental conditions, such as reaction temperature, residence time, and the molar ratio of reactants. All the conditions were determined by Box-Behnken experimental design and the response surface method based on the preferred range selected by single-factor experiments. For comparison, NPG was synthesized by condensation disproportionation of the same reactants in a batch reactor. The results showed that higher NPG yield and shorter residence time were achieved in a continuous-flow microreactor than in a batch reactor when the other experimental conditions were similar.     

Performance Characteristics of a Laser Initiated Microdetonator

The test results of 320 units of a laser initiated microdetonator are summarized. The commercially fabricated units used a lead styphnate/lead azide/HMX (1 mg/17.5 mg/13.5 mg) explosive train design contained in a miniature aluminum can that was capped with a glass-metal seal window. The test parameters were the laser energy, temperature, laser pulse duration, laser wavelength and nuclear radiation (5 × 10⁶ rad of 1 MeV gamma rays). The performance parameters were the laser energy for ignition and the actuation response time.     

A New Approach to Counteracting Chromatographic Electrophoresis

ABSTRACT

A newly designed apparatus, the J-column, was used to implement the concept of counteracting chromatographic electrophoresis (CACE). The process of separation and focusing was visualized by a model mixture of hemoglobin and ferritin. According to isoelectric focusing analysis, hemoglobin can be purified from the hemolysate mixture by CACE on a J-column.     

A New Approach to Scaling Up Electrophoresis

Abstract

Free Flow Electrophoresis (FFE) has been utilized for the separation of proteins and cells for many years, and has evolved into the most promising method of continuous separation. One of the major drawbacks inherent with FFE, however, is the thermal convection due to Joule heating which occurs whenever current is passed through a conducting solution. To provide efficient heat dissipation, the size of FFE units is restricted, which limits sample throughput. A new type of FFE design, which internally cools the separation unit by passing water through capillary tubes, has been developed and tested. Results of separations of dyes are presented, using a bed 1/4 inch thick which maintains efficient cooling.     

新型环保型阴极电泳漆的研制

通过对丙烯酸环氧树脂接枝聚合物及半封闭甲苯二异氰酸酯的合成反应条件的研究,得到性能优异的无铅环保型阴极电泳漆.     

有机硅烷前处理与电泳涂装性能的研究

介绍了一种有机硅烷钝化液,它可以用于阴极电泳的前处理。对钝化膜的耐碱性及电泳后漆膜的附着力、耐冲击、耐腐蚀等性能进行测试,并与使用传统磷化液前处理的电泳工艺进行比较。结果表明:经有机硅烷前处理后的阴极电泳漆膜的性能符合相关行业要求,证明有机硅烷处理和阴极电泳漆完全配套,可以在汽车及相关行业中推广应用。     

高效毛细管区带电泳技术测定泛酸钙的含量

介绍了以硼砂－ 磷酸盐为缓冲溶液,应用高效毛细管区带电泳技术测定泛酸钙含量的方法。平均回收率为９５ ．６ ％ 。方法简便、快速、准确、重现性好。     

新型智能石墨消解仪土壤消解的优化研究

采用三种不同的消解程序对土壤标准物质GSS-16、GSS-24和GSS-28消解优化比较,结果表明:三种程序对土壤重金属铜、铅、锌都有很好的消解回收,但是第一种程序对重金属镍的回收最好,对土壤标准物质GSS-16、GSS-24和GSS-28的回收率分别为96.0%、100.1%和94.9%。综上,可以采用第一种消解程序消解土壤以达到更佳的消解效果。     

新型管道测径清管器

管道测径清管已成为新建管线验收的重要手段之一,并受到管道业主的高度认可。文章介绍了管道测径清管器的用途,重点论述了目前所使用的常规测径清管器存在的弊端,并有针对性地提出了新型测径清管器的研究方向、具体研究内容。最后阐述了测径清管器的使用流程。     

浅谈液相色谱仪的管理与维护

介绍了高效液相色谱仪的结构组成和发展,阐述了流路泵系统、色谱柱和几种常用检测器的管理与维护,为液相色谱分析工作,提供一些值得借鉴的仪器管理与维护经验。     

高效毛细管电泳法测定刺五加中紫丁香苷的含量

建立刺五加中紫丁香苷的含量的测定方法。采用高效毛细管电泳法,运行缓冲液为15 mmol.L-1磷酸二氢钠-20 mmol.L-1硼砂溶液(pH8.5)。运行电压25 kV;柱温25℃;检测波长254 nm。紫丁香苷在0.012 9～0.258mg.mL-1(r0.999 8)线性关系良好,加样回收率为99.78%,RSD1.09%。方法操作简便,快速,重现性好,适用于刺五加的质量控制。     

高效毛细管电泳法测定苦参中生物碱的含量

为了更好地对苦参中生物碱的含量进行测定,需要选取有效的检测方法,而高效毛细管电泳法近年来发挥着重要的作用。采用此方法使苦参中的生物碱得到了有效的分析,且苦参碱回收率也较高。所以,高效毛细管电泳法是一种非常有效的生物碱测定方法。