Experimental and modeling approach to study sorption of dissolved hydrophobic organic contaminants to microbial biofilms

A biofilm reactor was developed to investigate the sorption of polycyclic aromatic hydrocarbons (PAH) as model compounds for hydrophobic organic contaminants (HOC) to intact microbial biofilms at environmentally realistic concentrations. When operated as a differential column batch reactor, the system can be used to study the thermodynamics as well as the kinetics of the exchange of HOC between an aqueous phase and microbial biofilms. Organic carbon normalized partition coefficients (K(oc)) for phenanthrene, fluoranthene and pyrene were at the lower end of those known for other organic sorbents. Intra-biofilm diffusion coefficients (D) were calculated from decrease in solute concentration over time using a model for diffusion through a plane sheet and ranged from 0.23 to 0.45x10(-9)cm(2)s(-1) for the three PAH. These diffusion coefficients are about four orders of magnitude lower than those reported in literature for free aqueous solution. These data and the experimental approach presented here are useful to assess the importance of microbial biofilms for exchange processes of HOC in heterogeneous systems such as water distribution systems, membranes and aquifers, sewer systems or surface soils.     

Measurements and modeling of pesticide persistence in soil at the catchment scale

Degradation of pesticides in soils is both spatially variable and also one of the most sensitive factors determining losses to surface water and groundwater. To date, no general guidance is available on suitable approaches for dealing with spatial variation in pesticide degradation in catchment or regional scale modeling applications. The purpose of the study was therefore to study the influence of various soil physical, chemical and microbiological characteristics on pesticide persistence in the contrasting cultivated soils found in a small (13 km²) agricultural catchment in Sweden and to develop and test a simple model approach that could support catchment scale modeling. Persistence of bentazone, glyphosate and isoproturon was investigated in laboratory incubation experiments. Degradation rate constants were highly variable with coefficients of variation ranging between 42 and 64% for the three herbicides. Multiple linear regression analysis and Mallows Cp statistic were employed to select the best set of independent parameters accounting for the variation in degradation. Soil pH and the proportion of active microorganisms (r) together explained 69% of the variation in the bentazone degradation rate constant; the Freundlich sorption co-efficient (K_f) and soil laccase activity together explained 88% of the variation in degradation rate of glyphosate, while soil pH was a significant predictor (p < 0.05) for isoproturon persistence. However, correlations between many potential predictor variables made clear interpretations of the statistical analysis difficult. Multiplicative models based on two predictors chosen ‘a priori’, one accounting for microbial activity (e.g. microbial respiration, laccase activity or the surrogate variable soil organic carbon, SOC) and one accounting for the effects of sorption on bioavailability, showed promise to support predictions of degradation for large-scale modeling applications, explaining up to 50% of the variation in herbicide persistence.     

Slow and very slow desorption of organic compounds from sediment: influence of sorbate planarity

The kinetics of desorption of in situ chlorobenzenes, PAHs, and PCBs from four different sediments was studied employing Tenax beads as an infinite sink for sorbates. Rate constants for slow desorption were 2.9+/-0.4 x 10(-2) x h(-1), irrespective of the extent of sorbate planarity. Rate constants for very slow desorption were 2.1+/-0.5 x 10(-4) and 6.7+/-1.4 x 10(-4) x h(-1) for planar and non-planar compounds, respectively. Comparison with literature data suggests a priori estimates for rate constants for slow desorption to be 3 x 10(-2) x h(-1), and to be 2 x 10(-4) and 7 x 10(-4) x h(-1) for very slow desorption of planar and non-planar compounds, respectively. The ratio between the fractions in the very slowly desorbing domain and the rapidly desorbing domain was 15-38 for planar compounds which is higher than for non-planar compounds for which the ratio was 2.8-5.2. The ratio between the fractions in the slowly desorbing domain and the rapidly desorbing domain was 1.3-1.8 and independent of the sorbate planarity. The difference in influence of sorbate planarity on the very slowly desorbing domain as compared to the slowly desorbing domain points to different environments for the slowly and the very slowly desorbing fractions.     

Transport of selected bacterial pathogens in agricultural soil and quartz sand

The protection of groundwater supplies from microbial contamination necessitates a solid understanding of the key factors controlling the migration and retention of pathogenic organisms through the subsurface environment. The transport behavior of five waterborne pathogens was examined using laboratory-scale columns packed with clean quartz at two solution ionic strengths (10 mM and 30 mM). Escherichia coli O157:H7 and Yersinia enterocolitica were selected as representative Gram-negative pathogens, Enterococcus faecalis was selected as a representative Gram-positive organism, and two cyanobacteria (Microcystis aeruginosa and Anabaena flos-aquae) were also studied. The five organisms exhibit differing attachment efficiencies to the quartz sand. The surface (zeta) potential of the microorganisms was characterized over a broad range of pH values (2-8) at two ionic strengths (10 mM and 30 mM). These measurements are used to evaluate the observed attachment behavior within the context of the DLVO theory of colloidal stability. To better understand the possible link between bacterial transport in model quartz sand systems and natural soil matrices, additional experiments were conducted with two of the selected organisms using columns packed with loamy sand obtained from an agricultural field. This investigation highlights the need for further characterization of waterborne pathogen surface properties and transport behavior over a broader range of environmentally relevant conditions.     

城市文化遗产保护的交通政策研究——北京旧城实例分析

首先阐述了笔者对城市文化遗产保护的理解,指出城市文化遗产是超越城市本身的人类共同的财富,并且认为保护的目的在于更好的发展;其次分析了交通政策与城市文化遗产保护的关系,指出交通建设是城市更新的主要方式,并且认为历史保护是制定交通政策的重要因素,同时交通政策对历史保护具有重要的作用;最后以自己参与完成的《北京交通发展纲要研究》为基础,对北京旧城交通政策进行了实例分析。     

Transport of two metal oxide nanoparticles in saturated granular porous media: role of water chemistry and particle coating

The growing use of nanosized titanium dioxide (nTiO₂) and zinc oxide (nZnO) in a large number of commercial products raises concerns regarding their release and subsequent mobility in natural aquatic environments. Laboratory-scale sand-packed column experiments were conducted with bare and polymer-coated nTiO₂ and nZnO to improve our understanding of the mobility of these nanoparticles in natural or engineered water saturated granular systems. The nanoparticles are characterized over a range of environmentally relevant water chemistries using multiple complimentary techniques: dynamic light scattering, nanoparticle tracking analysis, transmission electron microscopy, and scanning electron microscopy. Overall, bare (uncoated) nanoparticles exhibit high retention within the water saturated granular matrix at solution ionic strengths (IS) as low as 0.1 mM NaNO₃ for bare nTiO₂ and 0.01 mM NaNO₃ for bare nZnO. Bare nTiO₂ and nZnO also display dynamic (time-dependent) deposition behaviors under selected conditions. In contrast, the polymer-coated nanoparticles are much less likely to aggregate and exhibit significant transport potential at IS as high as 100 mM NaNO₃ or 3 mM CaCl₂. These findings illustrate the importance of considering the extent and type of surface modification when evaluating metal oxide contamination potential in granular aquatic environments.     

面向新世纪的城市交通方式结构选择—南京城市交通发展战略研究实践

本文结合南京城市交通发展战略研究的实践,分析了交通方式结构的主要决定因素和发展规律,并在此基础上简要介绍了南京远景交通方式结构的确定过程。     

Contribution of Miscanthus x giganteus root exudates to the biostimulation of PAH degradation: an in vitro study

Phytoremediation is considered as a promising and cost-effective method to enhance bioremediation of polluted soils. Exudation of plant root secondary metabolites similar to organic pollutants may induce the expression of microbial degradative enzymes and favour cometabolism of xenobiotics. We investigated the contribution of Miscanthus x giganteus root exudates in the biostimulation of PAH-degradation. This perennial grass was chosen because of its capability to grow on polluted soils and its high biomass production for non-food purposes. First, the impact of cometabolism phenomena was evaluated on the selective enrichment of pyrene-degrading bacterial consortia. The identification of each isolated strains following incubation with pyrene only, “pyrene + phenanthrene”, “pyrene + salycilate” or “pyrene + diesel fuel” showed a varying bacterial diversity and pyrene-degrading ability, depending on the co-substrate used. Then, a microplate assay was designed, based on the simultaneous measurement of bacterial consortia growth and degradation activity, in the presence of PAH and total root exudates. Results showed that i) the addition of root exudates was efficient for promoting bacterial growth, ii) but a selective enrichment of PAH-degraders compared to aliphatic ones could be clearly demonstrated, thereby conducing to an enhanced PAH catabolism. The identification of plant secondary metabolites showed the presence of a broad range of flavonoid-derived compounds that could play a role in cometabolic processes. Microplate assays with the two major molecules, quercetin and rutin, suggested a partial involvement of these compounds in biostimulation processes. Further investigations with the other identified secondary metabolites (apigenin, isovitexin, catechin, gallic and caffeic acid) should provide more information on the exudate-PAH cometabolic degradation phenomenon.     

Sorption of perfluorooctane sulfonate and perfluorooctanoate on activated carbons and resin: Kinetic and isotherm study

Perfluorooctane sulfonate (PFOS) and perfluorooctanoate (PFOA) have increasingly attracted global concerns in recent years due to their global distribution, persistence, strong bioaccumulation and potential toxicity. The feasibility of using powder activated carbon (PAC), granular activated carbon (GAC) and anion-exchange resin (AI400) to remove PFOS and PFOA from water was investigated with regard to their sorption kinetics and isotherms. Sorption kinetic results show that the adsorbent size influenced greatly the sorption velocity, and both the GAC and AI400 required over 168 h to achieve the equilibrium, much longer than 4 h for the PAC. Two kinetic models were adopted to describe the experimental data, and the pseudo-second-order model well described the sorption of PFOS and PFOA on the three adsorbents. The sorption isotherms show that the GAC had the lowest sorption capacity both for PFOS and PFOA among the three adsorbents, while the PAC and AI400 possessed the highest sorption capacity of 1.04 mmol g⁻¹ for PFOS and 2.92 mmol g⁻¹ for PFOA according to the Langmuir fitting. Based on the sorption behaviors and the characteristics of the adsorbents and adsorbates, ion exchange and electrostatic interaction as well as hydrophobic interaction were deduced to be involved in the sorption, and some hemi-micelles and micelles possibly formed in the intraparticle pores.     

Transport and deposition of sediment-associated Escherichia coli in natural streams

The association of microorganisms with sediment particles is one of the primary complicating factors in assessing microbial fate in aquatic systems. The literature indicates that the majority of enteric bacteria in aquatic systems are associated with sediments and that these associations influence their survival and transport characteristics. Yet, the nature of these associations has not been fully characterized. In this study, a combination of field experiments and mathematical modeling were used to better understand the processes which control the fate and transport of enteric bacteria in alluvial streams. An experimental procedure, involving the use of a tracer-bacteria, was developed to simulate the transport and deposition of bacteria-laden bed sediments in a small alluvial stream during steady flow conditions. The experimental data and mathematical model were used to determine dispersion coefficients, deposition rates, and partitioning coefficients for sediment-associated bacteria in two natural streams. The results provided evidence that bacterial adsorption can be modeled as an irreversible process in freshwater environments. Net settling velocities of fine sediments and associated bacteria were typically two orders of magnitude lower than those predicted from Stokes equation, due to re-entrainment of settled particles. The information presented in this study will further the development of representative microbial water quality models.     

Nonlinear eddy viscosity modeling and experimental study of jet spreading rates

Indoor airflow pattern is strongly influenced by turbulent shear and turbulent normal stresses that are responsible for entrainment effects and turbulence‐driven secondary motion. Therefore, an accurate prediction of room airflows requires reliable modeling of these turbulent quantities. The most widely used turbulence models include RANS‐based models that provide quick solutions but are known to fail in turbulent free shear and wall‐affected flows. In order to cope with this deficiency, this study presents a nonlinear k‐ε turbulence model and evaluates it along with linear k‐ε models for an indoor isothermal linear diffuser jet flow measured in two model rooms using PIV. The results show that the flow contains a free jet near the inlet region and a wall‐affected region downstream where the jet is pushed toward the ceiling by entrainment through the well‐known Coanda effect. The CFD results show that an accurate prediction of the entrainment process is very important and that the nonlinear eddy viscosity model is able to predict the turbulence‐driven secondary motions. Furthermore, turbulence models that are calibrated for high Reynolds free shear layer flows were not able to reproduce the measured velocity distributions, and it is suggested that the model constants of turbulence models should be adjusted before they are used for room airflow simulations.     

大都市近郊区城市功能转型与交通发展互动关系研究--以上海市宝山区为例

大都市郊区的城市功能转型与交通发展有着密切的关系。该文以上海市宝山区为例,以大量翔实的数据为支撑,实证研究了从1988年到2011年交通发展与城市功能转型的互动演化过程,从城市功能转变、交通系统作用、政府政策行动和应对举措入手,深入分析了宝山区从港口工业城市向“宜居城市”转型的动力机制．为当前我国城市化发展背景下广泛展开的大都市近郊区转型提供借鉴。     

Transport and retention of a bacteriophage and microspheres in saturated, angular porous media: effects of ionic strength and grain size

Eight saturated column experiments were conducted to examine the effects of solution chemistry and grain size on the transport of colloids through crushed silica sand. Two sizes of colloids, 0.025-μm bacteriophage (MS-2) and 1.5-μm carboxylated microspheres, were used as surrogates for the transport of pathogenic viruses and bacteria, respectively. Increasing the Ca²⁺ concentration from 1 to 4.8 mM (along with background monovalent ions) resulted in complete attenuation (>6-log decrease in C/C₀) of MS-2, but caused only a 1-log reduction (C/C₀ = 0.1) in the concentration of the microspheres. Decreasing grain size from medium sand (d₅₀ = 0.70 mm) to fine sand (d₅₀ = 0.34 mm) resulted in substantial decreases in effluent concentrations of both the MS-2 (5-log decrease) and microspheres (>2.5-log decrease). Comparison of observed colloid retention to that predicted by a recently published correlation equation for colloid filtration revealed that the model can considerably underpredict (by 4 orders of magnitude or more) colloid retention by angular sand over distances as short as 20 cm. This indicates that state-of-the-art colloid filtration models are still limited in applicability to natural systems.     

Estimating sludge loadings to land based on trace metal sorption in soil: effect of dissolved organo-metallic complexes

This paper describes the results of research examining the effect of dissolved organo-metallic complexes of copper (Cu) and zinc (Zn) from sewage sludge leachate on sorption by a humic-gley soil A-horizon, and the influence of such complexes on resultant sludge loading estimates. Sorption was described with Linear, Freundlich or Langmuir equations, and compared between a sample of sludge leachate (containing 97.4% of Cu and 63.2% of Zn as dissolved organo-metallic complexes) and a reference solution (which mimicked the leachate, except for a lack of dissolved organic material). This comparison revealed that dissolved organo-metallic complexes significantly depressed Cu and Zn sorption in the study soil. The isotherm equations were then used to estimate sludge-derived Cu and Zn loadings to soil in order to result in an "allowable" output concentration from the soil solution to the surrounding environment. These loadings, together with soil bulk density and "availability" of sludge Cu and Zn, were incorporated in a preliminary model to estimate sludge application rates which are acceptable in terms of off-site movement of these metals through leaching losses. In the absence of dissolved organo-metallic complexes (sorption from the reference solution), levels of Cu and Zn sorption in the study soil indicated a sludge application rate of approximately 3500 kg/ha. However, when Cu and Zn sorption from the sludge leachate with dissolved organo-metallic complexes was considered, calculated loading rates were reduced to approximately 690 kg-sludge/ha. This suggests that for sludge loading estimates based on soil sorption characteristics to be relevant to environmental protection, the sorption depressing effect of dissolved organo-metallic complexes should be quantitatively considered.     

Sorption and biodegradation of sulfonamide antibiotics by activated sludge: experimental assessment using batch data obtained under aerobic conditions

This study investigated the adsorption, desorption, and biodegradation characteristics of sulfonamide antibiotics in the presence of activated sludge with and without being subjected to NaN₃ biocide. Batch experiments were conducted and the relative contributions of adsorption and biodegradation to the observed removal of sulfonamide antibiotics were determined. Three sulfonamide antibiotics including sulfamethoxazole (SMX), sulfadimethoxine (SDM), and sulfamonomethoxine (SMM), which had been detected in the influent and the activated sludge of wastewater treatment plants (WWTP) in Taiwan, were selected for this study. Experimental results showed that the antibiotic compounds were removed via sorption and biodegradation by the activated sludge, though biodegradation was inhibited in the first 12 h possibly due to competitive inhibition of xenobiotic oxidation by readily biodegradable substances. The affinity of sulfonamides to sterilized sludge was in the order of SDM > SMM > SMX. The sulfonamides existed predominantly as anions at the study pH of 6.8, which resulted in a low level of adsorption to the activated sludge. The adsorption/desorption isotherms were of a linear form, as well described by the Freundlich isotherm with the n value approximating unity. The linear distribution coefficients (K_d) were determined from batch equilibrium experiments with values of 28.6 ± 1.9, 55.7 ± 2.2, and 110.0 ± 4.6 mL/g for SMX, SMM, and SDM, respectively. SMX, SMM, and SDM desorb reversibly from the activated sludge leaving behind on the solids 0.9%, 1.6%, and 5.2% of the original sorption dose of 100 μg/L. The sorbed antibiotics can be introduced into the environment if no further treatments were employed to remove them from the biomass.     

The Integration of Air and Rail Technologies: Shanghai's Hongqiao Integrated Transport Hub

This paper examines the different approaches to integrating air and rail technologies and their services at the Shanghai Hongqiao Integrated Transport Hub (“the Hongqiao Hub”) in China. Even though the Hongqiao Hub has the advantage of having both an airport and a railway station located at the same place, its level of air-rail integration remains at a low or moderate level. By analyzing existing air and rail services and potential travel demands, this paper recommends further streamlining the relationships among the Shanghai Airport Authority, the Shanghai Railway Bureau, and airline companies by fostering air-rail integration through the improvement of joint ticketing and code-sharing, direct luggage transfer, the provision of the fast and convenient connection from the Hongqiao International Airport to the Pudong International Airport, and air-rail linkage service area expansion.     

Laboratory tests on the impact of superabsorbent polymers on transformation and sorption of xenobiotics in soil taking 14C-imazalil as an example

Due to water scarcity, the agricultural production in arid areas is dependent on a sustainable irrigation management. In order to optimize irrigation systems, the application of superabsorbent polymers (SAP) as soil amendments, frequently studied within the last years, may be an appropriate measure to enhance the water holding capacity and the plant-available water in poor arable soils. These persistent polymers are also able to reduce heavy metal and salt stress to crops by accumulating those inorganic compounds. However, the impact of SAP on fate and behavior of organic xenobiotics in soil is unknown. Therefore, transformation and sorption of the model substance ¹⁴C-imazalil were monitored without and with SAP amendment in silty sand and sand soil under laboratory conditions.Within the 100-d incubation period, the transformation of ¹⁴C-imazalil was not substantially affected by the SAP amendment even though the microbial activity increased considerably. In the silty sand soil, extractable residues dropped from 90% to 45% without and from 96% to 46% with SAP amendment. Non-extractable residues continuously increased up to 49% and 35% while mineralization reached 6% and 5%, respectively. In the sand soil, characterized by its lower microbial activity and lower organic carbon content, extractable residues merely dropped from 99% to 81% and from 100% to 85% while non-extractable residues increased from 2% to 14% and 1% to 10%, respectively. Mineralization was lower than 2%. The increased microbial activity, usually promoting transformation processes of xenobiotics, was compensated by the enhanced sorption in the amended soils revealed by the increase of soil/water distribution coefficients (K_d) of 26 to 42 L kg^− 1 for the silty sand and 6 to 25 L kg^− 1 for the sand, respectively.     

Mathematical modeling and experimental study on vertical temperature distribution of hybrid ventilation in an atrium building

An extended Gebhart-Block model for large space buildings is proposed in this paper, which is used to predict the vertical temperature distribution of hybrid ventilation. Natural ventilation coupled with air-conditioning is described with a mathematical model. The indoor thermal environment formed by the air-conditioning and the natural ventilation can also be conducted for the potential analysis and optimal use of the natural ventilation. Experiments were carried out in an atrium building in Shanghai Research Institute of Building Sciences. This paper describes aforementioned model, and it is found that the calculation results implying this model agree well with the measurement data.     

Acute health effects of desktop 3D printing (fused deposition modeling) using acrylonitrile butadiene styrene and polylactic acid materials: An experimental exposure study in human volunteers

3D printers are increasingly run at home. Nanoparticle emissions from those printers have been reported, which raises the question whether adverse health effects from ultrafine particles (UFP) can be elicited by 3D printers. We exposed 26 healthy adults in a single‐blinded, randomized, cross‐over design to emissions of a desktop 3D printer using fused deposition modeling (FDM) for 1 hour (high UFP‐emitting acrylonitrile butadiene styrene [ABS] vs low‐emitting polylactic acid [PLA]). Before and after exposures, cytokines (IL‐1β, IL‐6, TNF‐α, INF‐γ) and ECP in nasal secretions, exhaled nitric oxide (FeNO), urinary 8‐isoprostaglandin F_2α (8‐iso PGF_2α), and self‐reported symptoms were assessed. The exposures had no significant differential effect on 8‐iso PGF_2α and nasal biomarkers. However, there was a difference (P < .05) in the time course of FeNO, with higher levels after ABS exposure. Moreover, indisposition and odor nuisance were increased for ABS exposure. These data suggest that 1 hour of exposure to 3D printer emissions had no acute effect on inflammatory markers in nasal secretions and urine. The slight relative increase in FeNO after ABS printing compared to PLA might be due to eosinophilic inflammation from inhaled UFP particles. This possibility should be investigated in further studies using additional biomarkers and longer observation periods.     

非饱和土水汽迁移与相变：两类“锅盖效应”的试验研究

“锅盖效应”定义为：不透水覆盖层下土体含水率大幅提高甚至饱和。最新的理论研究将“锅盖效应”分为两类,第一类由非饱和土内水气冷凝引起,第二类是冻结条件下由气态水迁移引起。为试验验证两类“锅盖效应”,研究其内在机理,利用新研制的非饱和冻土水汽迁移试验仪,对不同初始含水率的试样开展不同温度条件的水汽迁移试验。试验结果表明：冻结和未冻结两种状态均能使钙质砂试样顶部含水率增加,但冻结状态下的增加幅度显著。冻结状态下,含水率峰值位置与冻结锋面大致相同,且初始含水率越大,试样顶部和冻结锋面处的含水率增加越显著,降温速率越小,气态水迁移越显著;初始含水率增加也能使未冻结状态下的钙质砂试样顶部含水率增加,并且温度梯度对气态水迁移有一定的抑制作用,温度梯度越小,抑制作用越明显。试验结果很好地验证了两类“锅盖效应”的理论。